################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AccIII AclI AcyI Aor51HI AscI AspI BbeI BlpI Bpu1102I BsaHI BseAI BsiEI BspEI BspHI BstBI CelII CpoI EagI EclXI Eco47III Eco52I EcoT22I FseI Hin1I KasI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 1508 GGTACC G^GTACC AflII 5097 CTTAAG C^TTAAG AgeI 1511 ACCGGT A^CCGGT AhdI 907 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 438 GTGCAC G^TGCAC ApaLI 438 GTGCAC G^TGCAC Asp718I 1508 GGTACC G^GTACC AspEI 907 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 919 TGCGCA TGC^GCA AvrII 1969 CCTAGG C^CTAGG BamHI 1631 GGATCC G^GATCC BbvCI 2816 CCTCAGC CCTCAGC(-5/-2) BcgI 1628 GCAGGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4782 TGATCA T^GATCA BfrI 5097 CTTAAG C^TTAAG BlnI 1969 CCTAGG C^CTAGG BsaBI 3750 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 1752 CGTACG C^GTACG BspDI 2843 ATCGAT AT^CGAT BspLU11I 3566 ACATGT A^CATGT BssHII 1480 GCGCGC G^CGCGC Bst1107I 2742 GTATAC GTA^TAC BstEII 1596 GGTCACC G^GT[ACGT]ACC BstPI 1596 GGTCACC G^GT[ACGT]ACC BstXI 4579 CCAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2742 GTATAC GTA^TAC ClaI 2843 ATCGAT AT^CGAT DrdI 546 GACCTATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 907 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1596 GGTCACC G^GT[ACGT]ACC FbaI 4782 TGATCA T^GATCA FspI 919 TGCGCA TGC^GCA HpaI 5062 GTTAAC GTT^AAC KpnI 1508 GGTACC GGTAC^C MamI 3750 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 5071 CCATGG C^CATGG PflMI 1352 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1511 ACCGGT A^CCGGT PshAI 2160 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 866 GAGCTC GAGCT^C SspI 3156 AATATT AAT^ATT SwaI 4673 ATTTAAAT ATTT^AAAT Van91I 1352 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 758 CCACAGCATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 2742 GTATAC GT^[AC][GT]AC 5055 GTAGAC AocI 381 CCTGAGG CC^T[ACGT]AGG 2657 CCTAAGG ApaI 120 GGGCCC GGGCC^C 1443 GGGCCC AseI 889 ATTAAT AT^TAAT 3331 ATTAAT AsnI 889 ATTAAT AT^TAAT 3331 ATTAAT Asp700I 144 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 975 GAACACATTC BalI 732 TGGCCA TGG^CCA 4860 TGGCCA BciVI 3188 GGATAC GTATCC(6/5) 1612 GTATCC BglII 356 AGATCT A^GATCT 4952 AGATCT BsaI 1959 GAGACC GGTCTC(1/5) 3661 GGTCTC BsaWI 1082 TCCGGT [AT]^CCGG[AT] 1511 ACCGGT BseRI 1773 GAGGAG GAGGAG(10/8) 3211 GAGGAG BsmI 452 GCATTC GAATGC(1/-1) 4422 GCATTC BstAPI 3926 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4219 GCAGCTGGTGC Bsu36I 381 CCTGAGG CC^T[ACGT]AGG 2657 CCTAAGG Eco81I 381 CCTGAGG CC^T[ACGT]AGG 2657 CCTAAGG EcoRI 1099 GAATTC G^AATTC 4989 GAATTC EcoRV 181 GATATC GAT^ATC 2551 GATATC HgaI 3971 GACGC GACGC(5/10) 4728 GACGC HincII 4725 GTTGAC GT[CT]^[GA]AC 5062 GTTAAC HindII 4725 GTTGAC GT[CT]^[GA]AC 5062 GTTAAC HindIII 631 AAGCTT A^AGCTT 4103 AAGCTT KspI 855 CCGCGG CCGC^GG 1291 CCGCGG MluNI 732 TGGCCA TGG^CCA 4860 TGGCCA MscI 732 TGGCCA TGG^CCA 4860 TGGCCA PshBI 889 ATTAAT AT^TAAT 3331 ATTAAT SacII 855 CCGCGG CCGC^GG 1291 CCGCGG SbfI 1104 CCTGCAGG CCTGCA^GG 1304 CCTGCAGG SmaI 797 CCCGGG CCC^GGG 1504 CCCGGG SmlI 715 CTCAAG C^T[CT][GA]AG 5097 CTTAAG SpeI 3181 ACTAGT A^CTAGT 4172 ACTAGT Sse8387I 1104 CCTGCAGG CCTGCA^GG 1304 CCTGCAGG XmaCI 797 CCCGGG C^CCGGG 1504 CCCGGG XmaI 797 CCCGGG C^CCGGG 1504 CCCGGG XmnI 144 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 975 GAACACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII