##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        Aor51HI     
AscI        AspI        BbeI        BlpI        Bpu1102I    
BsaHI       BseAI       BsiEI       BspEI       BspHI       
BstBI       CelII       CpoI        EagI        EclXI       
Eco47III    Eco52I      EcoT22I     FseI        Hin1I       
KasI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PmeI        Psp1406I    PvuI        
RcaI        RsrII       SalI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SphI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1508  GGTACC               G^GTACC
AflII        5097  CTTAAG               C^TTAAG
AgeI         1511  ACCGGT               A^CCGGT
AhdI          907  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        438  GTGCAC               G^TGCAC
ApaLI         438  GTGCAC               G^TGCAC
Asp718I      1508  GGTACC               G^GTACC
AspEI         907  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         919  TGCGCA               TGC^GCA
AvrII        1969  CCTAGG               C^CTAGG
BamHI        1631  GGATCC               G^GATCC
BbvCI        2816  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1628  GCAGGATCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         4782  TGATCA               T^GATCA
BfrI         5097  CTTAAG               C^TTAAG
BlnI         1969  CCTAGG               C^CTAGG
BsaBI        3750  GATACATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI        1752  CGTACG               C^GTACG
BspDI        2843  ATCGAT               AT^CGAT
BspLU11I     3566  ACATGT               A^CATGT
BssHII       1480  GCGCGC               G^CGCGC
Bst1107I     2742  GTATAC               GTA^TAC
BstEII       1596  GGTCACC              G^GT[ACGT]ACC
BstPI        1596  GGTCACC              G^GT[ACGT]ACC
BstXI        4579  CCAGCCCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2742  GTATAC               GTA^TAC
ClaI         2843  ATCGAT               AT^CGAT
DrdI          546  GACCTATGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      907  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1596  GGTCACC              G^GT[ACGT]ACC
FbaI         4782  TGATCA               T^GATCA
FspI          919  TGCGCA               TGC^GCA
HpaI         5062  GTTAAC               GTT^AAC
KpnI         1508  GGTACC               GGTAC^C
MamI         3750  GATACATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         5071  CCATGG               C^CATGG
PflMI        1352  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1511  ACCGGT               A^CCGGT
PshAI        2160  GACCTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          866  GAGCTC               GAGCT^C
SspI         3156  AATATT               AAT^ATT
SwaI         4673  ATTTAAAT             ATTT^AAAT
Van91I       1352  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          758  CCACAGCATCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI         2742  GTATAC               GT^[AC][GT]AC
             5055  GTAGAC              
AocI          381  CCTGAGG              CC^T[ACGT]AGG
             2657  CCTAAGG             
ApaI          120  GGGCCC               GGGCC^C
             1443  GGGCCC              
AseI          889  ATTAAT               AT^TAAT
             3331  ATTAAT              
AsnI          889  ATTAAT               AT^TAAT
             3331  ATTAAT              
Asp700I       144  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              975  GAACACATTC          
BalI          732  TGGCCA               TGG^CCA
             4860  TGGCCA              
BciVI        3188  GGATAC               GTATCC(6/5)
             1612  GTATCC              
BglII         356  AGATCT               A^GATCT
             4952  AGATCT              
BsaI         1959  GAGACC               GGTCTC(1/5)
             3661  GGTCTC              
BsaWI        1082  TCCGGT               [AT]^CCGG[AT]
             1511  ACCGGT              
BseRI        1773  GAGGAG               GAGGAG(10/8)
             3211  GAGGAG              
BsmI          452  GCATTC               GAATGC(1/-1)
             4422  GCATTC              
BstAPI       3926  GCAGAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4219  GCAGCTGGTGC         
Bsu36I        381  CCTGAGG              CC^T[ACGT]AGG
             2657  CCTAAGG             
Eco81I        381  CCTGAGG              CC^T[ACGT]AGG
             2657  CCTAAGG             
EcoRI        1099  GAATTC               G^AATTC
             4989  GAATTC              
EcoRV         181  GATATC               GAT^ATC
             2551  GATATC              
HgaI         3971  GACGC                GACGC(5/10)
             4728  GACGC               
HincII       4725  GTTGAC               GT[CT]^[GA]AC
             5062  GTTAAC              
HindII       4725  GTTGAC               GT[CT]^[GA]AC
             5062  GTTAAC              
HindIII       631  AAGCTT               A^AGCTT
             4103  AAGCTT              
KspI          855  CCGCGG               CCGC^GG
             1291  CCGCGG              
MluNI         732  TGGCCA               TGG^CCA
             4860  TGGCCA              
MscI          732  TGGCCA               TGG^CCA
             4860  TGGCCA              
PshBI         889  ATTAAT               AT^TAAT
             3331  ATTAAT              
SacII         855  CCGCGG               CCGC^GG
             1291  CCGCGG              
SbfI         1104  CCTGCAGG             CCTGCA^GG
             1304  CCTGCAGG            
SmaI          797  CCCGGG               CCC^GGG
             1504  CCCGGG              
SmlI          715  CTCAAG               C^T[CT][GA]AG
             5097  CTTAAG              
SpeI         3181  ACTAGT               A^CTAGT
             4172  ACTAGT              
Sse8387I     1104  CCTGCAGG             CCTGCA^GG
             1304  CCTGCAGG            
XmaCI         797  CCCGGG               C^CCGGG
             1504  CCCGGG              
XmaI          797  CCCGGG               C^CCGGG
             1504  CCCGGG              
XmnI          144  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              975  GAACACATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbrPI       
BbsI        BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaAI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DraIII      DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspBI       StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XbaI        
XhoII