################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AspEI AvrII BcgI BfrI BlnI BsaBI BsiWI BsmBI BspDI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI Eam1105I Eco47III EcoNI FseI HindIII HpaI MamI MfeI MunI NaeI NgoMIV NheI NspV PacI PinAI PmeI PvuI RsrII ScaI SexAI SfiI SfuI SnaBI SspI SwaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 1639 GACGTC GACGT^C Acc65I 2842 GGTACC G^GTACC AccIII 3190 TCCGGA T^CCGGA AclI 798 AACGTT AA^CGTT ApaI 4327 GGGCCC GGGCC^C Asp718I 2842 GGTACC G^GTACC AviII 1433 TGCGCA TGC^GCA BamHI 1631 GGATCC G^GATCC BbvCI 3298 GCTGAGG CCTCAGC(-5/-2) BglI 775 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4272 AGATCT A^GATCT BlpI 1353 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1353 GCTCAGC GC^T[ACGT]AGC BseAI 3190 TCCGGA T^CCGGA Bsp1407I 5438 TGTACA T^GTACA BspEI 3190 TCCGGA T^CCGGA BspHI 3260 TCATGA T^CATGA BsrBI 5405 CCGCTC CCGCTC(-3/-3) BsrGI 5438 TGTACA T^GTACA BssHII 1569 GCGCGC G^CGCGC BstEII 2363 GGTCACC G^GT[ACGT]ACC BstPI 2363 GGTCACC G^GT[ACGT]ACC CelII 1353 GCTCAGC GC^T[ACGT]AGC EagI 1341 CGGCCG C^GGCCG EclXI 1341 CGGCCG C^GGCCG Eco52I 1341 CGGCCG C^GGCCG EcoO65I 2363 GGTCACC G^GT[ACGT]ACC EcoRV 5606 GATATC GAT^ATC FspI 1433 TGCGCA TGC^GCA KpnI 2842 GGTACC GGTAC^C MluI 1223 ACGCGT A^CGCGT MroI 3190 TCCGGA T^CCGGA NcoI 2643 CCATGG C^CATGG NdeI 3335 CATATG CA^TATG NotI 1340 GCGGCCGC GC^GGCCGC NruI 2440 TCGCGA TCG^CGA PflMI 539 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 857 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 798 AACGTT AA^CGTT RcaI 3260 TCATGA T^CATGA SapI 1539 GCTCTTC GCTCTTC(1/4) SbfI 4795 CCTGCAGG CCTGCA^GG SgrAI 662 CGCCGGTG C[GA]^CCGG[CT]G SmaI 1182 CCCGGG CCC^GGG SpeI 2142 ACTAGT A^CTAGT Sse8387I 4795 CCTGCAGG CCTGCA^GG SspBI 5438 TGTACA T^GTACA Van91I 539 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1182 CCCGGG C^CCGGG XmaI 1182 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 1792 GTCGAC GT^[AC][GT]AC 5094 GTCGAC AocI 135 CCTCAGG CC^T[ACGT]AGG 4595 CCTCAGG AseI 2113 ATTAAT AT^TAAT 4702 ATTAAT AsnI 2113 ATTAAT AT^TAAT 4702 ATTAAT Asp700I 490 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4222 GAAGGCTTTC AspI 1038 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4853 GACTTGGTC BbeI 1186 GGCGCC GGCGC^C 1417 GGCGCC BbrPI 2812 CACGTG CAC^GTG 5473 CACGTG BciVI 815 GTATCC GTATCC(6/5) 3341 GTATCC BclI 3561 TGATCA T^GATCA 3594 TGATCA BsaWI 3190 TCCGGA [AT]^CCGG[AT] 3276 TCCGGT BsgI 4621 CTGCAC GTGCAG(16/14) 3153 GTGCAG BsiEI 1341 CGGCCG CG[GA][CT]^CG 1473 CGACCG BsmI 2571 GAATGC GAATGC(1/-1) 5716 GAATGC BssSI 4523 CACGAG CACGAG(-5/-1) 1279 CTCGTG Bsu36I 135 CCTCAGG CC^T[ACGT]AGG 4595 CCTCAGG Eco81I 135 CCTCAGG CC^T[ACGT]AGG 4595 CCTCAGG EcoRI 2261 GAATTC G^AATTC 4841 GAATTC FbaI 3561 TGATCA T^GATCA 3594 TGATCA KasI 1186 GGCGCC G^GCGCC 1417 GGCGCC KspI 1272 CCGCGG CCGC^GG 1574 CCGCGG NarI 1186 GGCGCC GG^CGCC 1417 GGCGCC PflFI 1038 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4853 GACTTGGTC PmaCI 2812 CACGTG CAC^GTG 5473 CACGTG PmlI 2812 CACGTG CAC^GTG 5473 CACGTG PshBI 2113 ATTAAT AT^TAAT 4702 ATTAAT SacII 1272 CCGCGG CCGC^GG 1574 CCGCGG SalI 1792 GTCGAC G^TCGAC 5094 GTCGAC SfoI 1186 GGCGCC GGC^GCC 1417 GGCGCC SphI 160 GCATGC GCATG^C 420 GCATGC Tth111I 1038 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4853 GACTTGGTC XmnI 490 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4222 GAAGGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PaeR7I PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoI XhoII