################################## # NO CUT ENZYMES ( 38 enzymes) # ################################## AgeI Aor51HI AscI AspI AviII AvrII BamHI BlnI BsiWI BspDI BstEII BstPI ClaI Eco47III EcoNI EcoO65I FseI FspI HindIII HpaI MluI NdeI NheI NotI NruI PacI PflFI PinAI PmeI PvuI SalI SbfI SfiI SgrAI SnaBI SpeI Sse8387I Tth111I ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 6653 GACGTC GACGT^C AccIII 2542 TCCGGA T^CCGGA AclI 3911 AACGTT AA^CGTT AflII 5988 CTTAAG C^TTAAG Alw44I 6205 GTGCAC G^TGCAC ApaLI 6205 GTGCAC G^TGCAC AseI 5355 ATTAAT AT^TAAT AsnI 5355 ATTAAT AT^TAAT BbeI 173 GGCGCC GGCGC^C BbvCI 241 CCTCAGC CCTCAGC(-5/-2) BfrI 5988 CTTAAG C^TTAAG BsaBI 1943 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2542 TCCGGA [AT]^CCGG[AT] BseAI 2542 TCCGGA T^CCGGA BsiEI 195 CGGCCG CG[GA][CT]^CG BsmBI 327 GAGACG CGTCTC(1/5) Bsp1407I 1378 TGTACA T^GTACA BspEI 2542 TCCGGA T^CCGGA BspMI 2482 ACCTGC ACCTGC(4/8) BsrBI 567 GAGCGG CCGCTC(-3/-3) BsrGI 1378 TGTACA T^GTACA BssSI 2985 CACGAG CACGAG(-5/-1) Bst1107I 7400 GTATAC GTA^TAC BstBI 625 TTCGAA TT^CGAA BstZ17I 7400 GTATAC GTA^TAC CpoI 2515 CGGTCCG CG^G[AT]CCG DraIII 3129 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 195 CGGCCG C^GGCCG EclXI 195 CGGCCG C^GGCCG Eco52I 195 CGGCCG C^GGCCG KasI 173 GGCGCC G^GCGCC KspI 258 CCGCGG CCGC^GG MamI 1943 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1419 CAATTG C^AATTG MroI 2542 TCCGGA T^CCGGA MunI 1419 CAATTG C^AATTG NarI 173 GGCGCC GG^CGCC NspV 625 TTCGAA TT^CGAA PshAI 5501 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5355 ATTAAT AT^TAAT Psp1406I 3911 AACGTT AA^CGTT RsrII 2515 CGGTCCG CG^G[AT]CCG SacII 258 CCGCGG CCGC^GG SapI 2075 GCTCTTC GCTCTTC(1/4) SfoI 173 GGCGCC GGC^GCC SfuI 625 TTCGAA TT^CGAA SmaI 6964 CCCGGG CCC^GGG SphI 5031 GCATGC GCATG^C SspBI 1378 TGTACA T^GTACA XbaI 5960 TCTAGA T^CTAGA XmaCI 6964 CCCGGG C^CCGGG XmaI 6964 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 173 GGCGCC G[GA]^CG[CT]C 6653 GACGTC ApaI 5020 GGGCCC GGGCC^C 5152 GGGCCC BalI 919 TGGCCA TGG^CCA 1801 TGGCCA BbrPI 2744 CACGTG CAC^GTG 6608 CACGTG BciVI 5171 GGATAC GTATCC(6/5) 798 GTATCC BclI 2876 TGATCA T^GATCA 6351 TGATCA BsaHI 173 GGCGCC G[GA]^CG[CT]C 6653 GACGTC BssHII 158 GCGCGC G^CGCGC 412 GCGCGC BstXI 181 CCACACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3039 CCAGCCTTCTGG DrdI 329 GACGCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1718 GACTCGAGTGTC FbaI 2876 TGATCA T^GATCA 6351 TGATCA Hin1I 173 GGCGCC G[GA]^CG[CT]C 6653 GACGTC MluNI 919 TGGCCA TGG^CCA 1801 TGGCCA MscI 919 TGGCCA TGG^CCA 1801 TGGCCA NaeI 170 GCCGGC GCC^GGC 6141 GCCGGC NgoMIV 170 GCCGGC G^CCGGC 6141 GCCGGC PmaCI 2744 CACGTG CAC^GTG 6608 CACGTG PmlI 2744 CACGTG CAC^GTG 6608 CACGTG SexAI 772 ACCTGGT A^CC[AT]GGT 993 ACCAGGT StuI 3744 AGGCCT AGG^CCT 5301 AGGCCT SwaI 467 ATTTAAAT ATTT^AAAT 4371 ATTTAAAT ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApoI Asp700I Asp718I AspEI AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PaeR7I PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII XmnI