Miyakogusa Predicted Gene

Lj3g3v3639920.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v3639920.1 Non Chatacterized Hit- tr|I1LP26|I1LP26_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.46889
PE,80.79,0,Eukaryotic DNA topoisomerase I, N-terminal DNA-binding
fragment,DNA topoisomerase I, DNA binding, eu,CUFF.46130.1
         (898 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma12g01630.1                                                      1236   0.0  
Glyma09g35730.1                                                      1212   0.0  
Glyma09g35730.2                                                       988   0.0  
Glyma12g01620.1                                                       147   4e-35

>Glyma12g01630.1 
          Length = 894

 Score = 1236 bits (3198), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 641/906 (70%), Positives = 699/906 (77%), Gaps = 20/906 (2%)

Query: 1   MAVEASDKPNLPDNF---DDEPLVFKRYTS-KKSQLNSEVRKSTSQSHDGPSYRQNSDGP 56
           MAVE SDKPNLP +F   DD P+++KRY+S KK+Q +SEVRKS+S SHDG SYRQ S+ P
Sbjct: 1   MAVETSDKPNLPHDFEDDDDGPVIYKRYSSSKKNQSHSEVRKSSSHSHDGQSYRQISNAP 60

Query: 57  SSNGKTSSMQKGNIVPSAKVTPPVKASVGISKASNSLGNASSAKLPLANSKLVSSRDKPK 116
           SS+G+TS +QKGN VPSAK +P VK SVGISKAS SL N SS KLP+ANSK  S  D+  
Sbjct: 61  SSSGQTSCLQKGNTVPSAKPSP-VKPSVGISKASKSLVNTSSTKLPVANSKSPSLGDR-- 117

Query: 117 TLSEQKMSINVK-EEKNIKNCSKX--XXXXXXXXHKPLSARLKVNSHHDNKTSPXXXXXX 173
               QKMS+++K E K I++ +K           +KPLS RLK NS+HDNK +       
Sbjct: 118 ----QKMSVDIKGEPKTIEHHAKNFVEDSEDDEDNKPLSFRLK-NSNHDNKVT---SVIA 169

Query: 174 XXXXXXXXXXIPLSVKINRNSNLATSSSNYDDSD-KKPISKVQKERQNGSGITIKQERAS 232
                     +PL+ K+ RNSNL T+SSN+DDS+ KKPISK+QKE++NGSG++ K +R S
Sbjct: 170 KKSSEDSDDDVPLAKKLYRNSNLGTTSSNHDDSNQKKPISKIQKEQKNGSGMSNKLDRPS 229

Query: 233 TLPVKRPIDNSNSLNSSMKKPKVSDSAASTKAKQVSGKSQPKIEXXXXXXXXVPISQRIK 292
            LP KRP+DNSNSL+ S+KK KV D  AS K+K VS K + K+E        +PISQRIK
Sbjct: 230 PLPAKRPLDNSNSLHPSVKKSKVLDPVASIKSKAVSVKHELKVEDDDDDDD-IPISQRIK 288

Query: 293 NXXXXXXXXXXXXXXXXXXXXXXXXGTTXXXXXXXXXXXXXXXXXXXXXXXXLLPSSGDG 352
                                    G+                         +LPSSGDG
Sbjct: 289 KSAMSGDKSSSMKKVLTKVTKVNKPGSKPFKKQIKKTVKKSGSGSEYSKSSKILPSSGDG 348

Query: 353 QKKWTTLVHNGVIFPPPYQPHRVKMLYKGKPVDLTADQEEVATMYAVMRDTDYMLKDKFK 412
           QKKWTTLVHNGVIFPPPYQPH VKMLYKG+PVDLT +QEEVATMYAVMRDT+YM KDKFK
Sbjct: 349 QKKWTTLVHNGVIFPPPYQPHGVKMLYKGRPVDLTPEQEEVATMYAVMRDTEYMQKDKFK 408

Query: 413 ENFWNDWRKLLGKNHVIQNLKDCDFTPIYDWCXXXXXXXXXXXXXXXXXXXXXXXXXXXX 472
           ENFWNDWRKLLG+NHVIQNLKDCDFTPIYDW                             
Sbjct: 409 ENFWNDWRKLLGRNHVIQNLKDCDFTPIYDWYQSEKDKKKQMTTEEKKALKEEKMKLEEK 468

Query: 473 XXWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPSDIVINIGKDAPIPECPI 532
             WAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLK+RI P DI INI KDAPIPECPI
Sbjct: 469 YMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIHPGDITINIAKDAPIPECPI 528

Query: 533 PGERWKEIRHDNTVTWLAFWNDPINPKLFKYVFLAASSSLKGQSDKEKYEKARMLKDYIG 592
           PGERWKEIRHDNTVTWLA+W+DPINPKLFKYVFLAASSSLKGQSDKEKYEKAR LKDYIG
Sbjct: 529 PGERWKEIRHDNTVTWLAYWSDPINPKLFKYVFLAASSSLKGQSDKEKYEKARTLKDYIG 588

Query: 593 NIRAAYTKDFTSKDITKQQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVTREA 652
           NIR+AYTKDFTSKDITKQQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVTRE 
Sbjct: 589 NIRSAYTKDFTSKDITKQQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVTREP 648

Query: 653 PNKLKFNFLGKDSIKYENTVEVELPVYNAILKFQKDKKGGDDLFDMLDTSKLNAHLKELM 712
           PNKLKFNFLGKDSIKYENTVEVELPVYNAILKFQKDKK GDDLFD LDTSKLNAHLKELM
Sbjct: 649 PNKLKFNFLGKDSIKYENTVEVELPVYNAILKFQKDKKPGDDLFDKLDTSKLNAHLKELM 708

Query: 713 PGLTAKVFRTFNASITLDDMLNKDTKDGDVGEKIVVYQHANKQVAIICNHQRSVSKSHDT 772
           PGLTAKVFRTFNASITLD MLNK+TKDGDVGEKIVVYQHANKQVAIICNHQRSVSKSH  
Sbjct: 709 PGLTAKVFRTFNASITLDVMLNKETKDGDVGEKIVVYQHANKQVAIICNHQRSVSKSHTA 768

Query: 773 QMTRLNEKLDELQAVLKELRTDLDRARXXXXXXXXXXXXXXXNLSSEALEKKISQTNAKI 832
           QM++L EK+DELQAVLKEL+TDLDRAR               NLS E LEKKISQTNAKI
Sbjct: 769 QMSKLTEKIDELQAVLKELKTDLDRARKGKPPSKSSDGKRKKNLSPEMLEKKISQTNAKI 828

Query: 833 EKMQRDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFTWAMDV 892
           EKMQRDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKF WAMDV
Sbjct: 829 EKMQRDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFCWAMDV 888

Query: 893 DPDFRF 898
           DPDFRF
Sbjct: 889 DPDFRF 894


>Glyma09g35730.1 
          Length = 892

 Score = 1212 bits (3136), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 644/906 (71%), Positives = 699/906 (77%), Gaps = 22/906 (2%)

Query: 1   MAVEASDKPNLPDNFDDE---PLVFKRYTS-KKSQLNSEVRKSTSQSHDGPSYRQNSDGP 56
           MAVE SDKPNLP +FDD+   P+++KRY+S KK+QL+SEVRKS+S SH G SYRQ SD P
Sbjct: 1   MAVETSDKPNLPHDFDDDDDGPVIYKRYSSSKKNQLHSEVRKSSSHSHAGQSYRQISDAP 60

Query: 57  SSNGKTSSMQKGNIVPSAKVTPPVKASVGISKASNSLGNASSAKLPLANSKLVSSRDKPK 116
           S NG+T SMQKG+ VPS K +P VK SV ISKAS SL N SS KLP+ANSK  S  DK  
Sbjct: 61  SLNGQTFSMQKGSTVPSPKPSP-VKPSVAISKASKSLVNTSSTKLPVANSKAPSLGDK-- 117

Query: 117 TLSEQKMSINVK-EEKNIKNCSKXXXXXXX--XXHKPLSARLKVNSHHDNKTSPXXXXXX 173
               QKMS++VK E K I+ C+K           +KPLS RLK NS+HDNK +P      
Sbjct: 118 ----QKMSVDVKVEPKYIERCAKNFVEDSEDDEDNKPLSFRLK-NSNHDNKATPVVVKKS 172

Query: 174 XXXXXXXXXXIPLSVKINRNSNLATSSSNYDDSD-KKPISKVQKERQNGSGITIKQERAS 232
                     +PL+ K+ RN NL T+SSN DDSD KKPISK+QKERQNG+G++ K++R S
Sbjct: 173 SEDSDDD---VPLAKKLYRNPNLGTTSSNRDDSDQKKPISKIQKERQNGAGLSNKKDRPS 229

Query: 233 TLPVKRPIDNSNSLNSSMKKPKVSDSAASTKAKQVSGKSQPKIEXXXXXXXXVPISQRIK 292
            LP KRP+DNS+SL++S+KKPKVSD  AS K K VS K + K+E        +PISQRIK
Sbjct: 230 PLPAKRPLDNSDSLHASVKKPKVSDPVASIKTKPVSVKHELKVEDDDDD---IPISQRIK 286

Query: 293 NXXXXXXXXXXXXXXXXXXXXXXXXGTTXXXXXXXXXXXXXXXXXXXXXXXXLLPSSGDG 352
                                    G+                         LLPSSGDG
Sbjct: 287 KSAMSGDKSSATKKLLTKVTKVNKSGSKPFKKQIKKTVKKSGSGSEYSKSTKLLPSSGDG 346

Query: 353 QKKWTTLVHNGVIFPPPYQPHRVKMLYKGKPVDLTADQEEVATMYAVMRDTDYMLKDKFK 412
           QKKWTTLVHNGVIFPPPYQPH VKMLYKG+PVDLT + EEVATMYAVMRDT+YM KD+FK
Sbjct: 347 QKKWTTLVHNGVIFPPPYQPHGVKMLYKGRPVDLTPELEEVATMYAVMRDTEYMQKDRFK 406

Query: 413 ENFWNDWRKLLGKNHVIQNLKDCDFTPIYDWCXXXXXXXXXXXXXXXXXXXXXXXXXXXX 472
           ENFWNDWRKLLG+NHVIQNLKDCDFTPIYDW                             
Sbjct: 407 ENFWNDWRKLLGRNHVIQNLKDCDFTPIYDWYQSEKEKKKQMTTEEKKALKEEKMKLEEK 466

Query: 473 XXWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPSDIVINIGKDAPIPECPI 532
             WAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRP DI INIGKDAPIPECPI
Sbjct: 467 YMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPGDITINIGKDAPIPECPI 526

Query: 533 PGERWKEIRHDNTVTWLAFWNDPINPKLFKYVFLAASSSLKGQSDKEKYEKARMLKDYIG 592
           PGE WKEIR+DNTVTWLA+W+DPINPKLFKYVFLAASSSLKGQSDKEKYEKARMLK+YIG
Sbjct: 527 PGESWKEIRNDNTVTWLAYWSDPINPKLFKYVFLAASSSLKGQSDKEKYEKARMLKNYIG 586

Query: 593 NIRAAYTKDFTSKDITKQQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVTREA 652
           NIR+AYTKDFTSKDITKQQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVTRE 
Sbjct: 587 NIRSAYTKDFTSKDITKQQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVTREP 646

Query: 653 PNKLKFNFLGKDSIKYENTVEVELPVYNAILKFQKDKKGGDDLFDMLDTSKLNAHLKELM 712
           PNKLKFNFLGKDSIKYENTVEVELPVYNAILKFQKDKK GDDLFD LDTSKLNAHLKELM
Sbjct: 647 PNKLKFNFLGKDSIKYENTVEVELPVYNAILKFQKDKKPGDDLFDQLDTSKLNAHLKELM 706

Query: 713 PGLTAKVFRTFNASITLDDMLNKDTKDGDVGEKIVVYQHANKQVAIICNHQRSVSKSHDT 772
           PGLTAKVFRTFNASITLD MLNK+TKDG+VGEKIVVYQHANKQVAIICNHQRSVSKSH  
Sbjct: 707 PGLTAKVFRTFNASITLDVMLNKETKDGEVGEKIVVYQHANKQVAIICNHQRSVSKSHTA 766

Query: 773 QMTRLNEKLDELQAVLKELRTDLDRARXXXXXXXXXXXXXXXNLSSEALEKKISQTNAKI 832
           QM++L EK+DELQAVLKEL+TDLDRAR               NL  E LEKKISQTNAKI
Sbjct: 767 QMSKLTEKIDELQAVLKELKTDLDRARKGKPPSKSSDGKRKKNLGPEMLEKKISQTNAKI 826

Query: 833 EKMQRDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFTWAMDV 892
           EKMQRDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKF WAMDV
Sbjct: 827 EKMQRDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFCWAMDV 886

Query: 893 DPDFRF 898
           DPDFRF
Sbjct: 887 DPDFRF 892


>Glyma09g35730.2 
          Length = 603

 Score =  988 bits (2555), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 474/554 (85%), Positives = 490/554 (88%)

Query: 345 LLPSSGDGQKKWTTLVHNGVIFPPPYQPHRVKMLYKGKPVDLTADQEEVATMYAVMRDTD 404
           LLPSSGDGQKKWTTLVHNGVIFPPPYQPH VKMLYKG+PVDLT + EEVATMYAVMRDT+
Sbjct: 50  LLPSSGDGQKKWTTLVHNGVIFPPPYQPHGVKMLYKGRPVDLTPELEEVATMYAVMRDTE 109

Query: 405 YMLKDKFKENFWNDWRKLLGKNHVIQNLKDCDFTPIYDWCXXXXXXXXXXXXXXXXXXXX 464
           YM KD+FKENFWNDWRKLLG+NHVIQNLKDCDFTPIYDW                     
Sbjct: 110 YMQKDRFKENFWNDWRKLLGRNHVIQNLKDCDFTPIYDWYQSEKEKKKQMTTEEKKALKE 169

Query: 465 XXXXXXXXXXWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPSDIVINIGKD 524
                     WAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRP DI INIGKD
Sbjct: 170 EKMKLEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPGDITINIGKD 229

Query: 525 APIPECPIPGERWKEIRHDNTVTWLAFWNDPINPKLFKYVFLAASSSLKGQSDKEKYEKA 584
           APIPECPIPGE WKEIR+DNTVTWLA+W+DPINPKLFKYVFLAASSSLKGQSDKEKYEKA
Sbjct: 230 APIPECPIPGESWKEIRNDNTVTWLAYWSDPINPKLFKYVFLAASSSLKGQSDKEKYEKA 289

Query: 585 RMLKDYIGNIRAAYTKDFTSKDITKQQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLK 644
           RMLK+YIGNIR+AYTKDFTSKDITKQQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLK
Sbjct: 290 RMLKNYIGNIRSAYTKDFTSKDITKQQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLK 349

Query: 645 VENVTREAPNKLKFNFLGKDSIKYENTVEVELPVYNAILKFQKDKKGGDDLFDMLDTSKL 704
           VENVTRE PNKLKFNFLGKDSIKYENTVEVELPVYNAILKFQKDKK GDDLFD LDTSKL
Sbjct: 350 VENVTREPPNKLKFNFLGKDSIKYENTVEVELPVYNAILKFQKDKKPGDDLFDQLDTSKL 409

Query: 705 NAHLKELMPGLTAKVFRTFNASITLDDMLNKDTKDGDVGEKIVVYQHANKQVAIICNHQR 764
           NAHLKELMPGLTAKVFRTFNASITLD MLNK+TKDG+VGEKIVVYQHANKQVAIICNHQR
Sbjct: 410 NAHLKELMPGLTAKVFRTFNASITLDVMLNKETKDGEVGEKIVVYQHANKQVAIICNHQR 469

Query: 765 SVSKSHDTQMTRLNEKLDELQAVLKELRTDLDRARXXXXXXXXXXXXXXXNLSSEALEKK 824
           SVSKSH  QM++L EK+DELQAVLKEL+TDLDRAR               NL  E LEKK
Sbjct: 470 SVSKSHTAQMSKLTEKIDELQAVLKELKTDLDRARKGKPPSKSSDGKRKKNLGPEMLEKK 529

Query: 825 ISQTNAKIEKMQRDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLA 884
           ISQTNAKIEKMQRDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLA
Sbjct: 530 ISQTNAKIEKMQRDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLA 589

Query: 885 KFTWAMDVDPDFRF 898
           KF WAMDVDPDFRF
Sbjct: 590 KFCWAMDVDPDFRF 603


>Glyma12g01620.1 
          Length = 252

 Score =  147 bits (372), Expect = 4e-35,   Method: Compositional matrix adjust.
 Identities = 70/95 (73%), Positives = 75/95 (78%), Gaps = 10/95 (10%)

Query: 349 SGDGQKKWTTLVHNGVIFPPPYQPHRVKMLYKGKPVDLTADQEEVATMYAVMRDTDYMLK 408
           +GDGQ K TTLVHN VIFPP YQPH VKMLYKGKP    A+QEE   +      TDYM K
Sbjct: 136 AGDGQNKRTTLVHNAVIFPP-YQPHGVKMLYKGKP----AEQEEPCEI-----QTDYMPK 185

Query: 409 DKFKENFWNDWRKLLGKNHVIQNLKDCDFTPIYDW 443
           D+FKENFWNDWRKLLG+NHVIQNLKDCDF PIYDW
Sbjct: 186 DRFKENFWNDWRKLLGRNHVIQNLKDCDFIPIYDW 220



 Score = 88.6 bits (218), Expect = 3e-17,   Method: Compositional matrix adjust.
 Identities = 73/144 (50%), Positives = 92/144 (63%), Gaps = 33/144 (22%)

Query: 1   MAVEASDKPNLPDNFDDE----PLVFKRYTS-KKSQLNSEVRKSTSQSHDGPSYRQNSDG 55
           MAV+ SD+PNL  +FDD+    P+++KRY+S KK+QL+S+VRKS+S SHDG    Q S+ 
Sbjct: 1   MAVKTSDEPNLAHDFDDDDDDGPVIYKRYSSSKKNQLHSDVRKSSSHSHDG----QISNA 56

Query: 56  PSSNGKTSSMQKGNIVPSAKVTPPVKASVGISKASNSLGNASSAKLPLANSKLVSSRDKP 115
           PS+             PS     PVK SVGIS ASNSL N SS KLP+AN K  S  DK 
Sbjct: 57  PSAK------------PS-----PVKPSVGISNASNSLINTSSMKLPVANPKSPSLGDK- 98

Query: 116 KTLSEQKMSINVKEE-KNIKNCSK 138
                QKMSI+VKE+ K+I  C+K
Sbjct: 99  -----QKMSIDVKEKSKSIDYCAK 117