Miyakogusa Predicted Gene

Lj5g3v2013950.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v2013950.1 Non Characterized Hit- tr|I1NGS1|I1NGS1_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.33443 PE,80,0,Cytochrome
b5-like heme/steroid binding domain,Cytochrome b5; Cyt-b5,Cytochrome
b5; MEMBRANE STEROID,CUFF.56496.1
         (218 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr6g054890.1 | cytochrome b5-like heme/steroid-binding domain...   261   3e-70
Medtr6g054890.2 | cytochrome b5-like heme/steroid-binding domain...   261   3e-70
Medtr1g100727.1 | cytochrome b5-like heme/steroid-binding domain...   259   2e-69
Medtr6g027160.2 | cytochrome b5-like heme/steroid-binding domain...   133   1e-31
Medtr6g027160.1 | cytochrome b5-like heme/steroid-binding domain...   133   1e-31
Medtr6g027160.3 | cytochrome b5-like heme/steroid-binding domain...   133   1e-31
Medtr3g105860.1 | cytochrome b5-like heme/steroid-binding domain...   115   2e-26
Medtr3g081290.1 | cytochrome b5-like heme/steroid-binding domain...    79   4e-15
Medtr6g027060.1 | membrane steroid-binding protein, putative | H...    72   5e-13

>Medtr6g054890.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:19600682-19605989 | 20130731
          Length = 235

 Score =  261 bits (668), Expect = 3e-70,   Method: Compositional matrix adjust.
 Identities = 138/231 (59%), Positives = 153/231 (66%), Gaps = 14/231 (6%)

Query: 1   MAVQQWETLKEAIVAYTGLSPXXXXXXXXXXXXIYYVVSGLFGSSPDHNRHQHRHIXXXX 60
           MA+Q W T KEAIV YTGLSP            +YY+++ LFGSS  H RH         
Sbjct: 1   MALQLWSTFKEAIVVYTGLSPTTFFTLLVILFTLYYIITSLFGSSDTHQRHGSTRDFAAE 60

Query: 61  XXXXXXXVQLGEITEEELKAYDGSDPAKPLLMAIKAQIYDVSQSRMFYGPGGPYALFAGK 120
                  VQ+GE+TE+ELK YDG++P KPLLMAIK QIYDVSQSRMFYGPGGPYALFAGK
Sbjct: 61  MEPLKPPVQIGEVTEDELKDYDGNNPDKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGK 120

Query: 121 DASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFSEKYVKVGTVKSNVXXXXXXXX 180
           DASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKF  KYVKVGT+K+ V        
Sbjct: 121 DASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMGKYVKVGTIKT-VPVTKPEST 179

Query: 181 XXXXXXXXRDVD-------------AAKPAENGPSETAAVKSDENVDVNKD 218
                   R VD             A+KP EN PSETA VKSDEN  ++ D
Sbjct: 180 GEPSESTSRGVDASSIPHENHDAAEASKPHENTPSETAPVKSDENTPLSVD 230


>Medtr6g054890.2 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:19600690-19606711 | 20130731
          Length = 235

 Score =  261 bits (668), Expect = 3e-70,   Method: Compositional matrix adjust.
 Identities = 138/231 (59%), Positives = 153/231 (66%), Gaps = 14/231 (6%)

Query: 1   MAVQQWETLKEAIVAYTGLSPXXXXXXXXXXXXIYYVVSGLFGSSPDHNRHQHRHIXXXX 60
           MA+Q W T KEAIV YTGLSP            +YY+++ LFGSS  H RH         
Sbjct: 1   MALQLWSTFKEAIVVYTGLSPTTFFTLLVILFTLYYIITSLFGSSDTHQRHGSTRDFAAE 60

Query: 61  XXXXXXXVQLGEITEEELKAYDGSDPAKPLLMAIKAQIYDVSQSRMFYGPGGPYALFAGK 120
                  VQ+GE+TE+ELK YDG++P KPLLMAIK QIYDVSQSRMFYGPGGPYALFAGK
Sbjct: 61  MEPLKPPVQIGEVTEDELKDYDGNNPDKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGK 120

Query: 121 DASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFSEKYVKVGTVKSNVXXXXXXXX 180
           DASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKF  KYVKVGT+K+ V        
Sbjct: 121 DASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMGKYVKVGTIKT-VPVTKPEST 179

Query: 181 XXXXXXXXRDVD-------------AAKPAENGPSETAAVKSDENVDVNKD 218
                   R VD             A+KP EN PSETA VKSDEN  ++ D
Sbjct: 180 GEPSESTSRGVDASSIPHENHDAAEASKPHENTPSETAPVKSDENTPLSVD 230


>Medtr1g100727.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr1:45659938-45662838 | 20130731
          Length = 220

 Score =  259 bits (662), Expect = 2e-69,   Method: Compositional matrix adjust.
 Identities = 139/220 (63%), Positives = 153/220 (69%), Gaps = 5/220 (2%)

Query: 2   AVQQWETLKEAIVAYTGLSPXXXXXXXXXXXXIYYVVSGLFGSSPDHNRHQHRHIXXXXX 61
            VQ WETLKE+IVAYTGLSP            +YYV+SGLFG S      Q R+      
Sbjct: 3   GVQLWETLKESIVAYTGLSPTTFFTILALLFAVYYVLSGLFGYSSSDLHPQTRNFQEEEL 62

Query: 62  XXXXXXVQLGEITEEELKAYDGSDPAKPLLMAIKAQIYDVSQSRMFYGPGGPYALFAGKD 121
                 VQLGEITEEELKAYDG+D  KPLLMAIK QIYDVSQSRMFYGPGGPYALFAGKD
Sbjct: 63  PPLRPPVQLGEITEEELKAYDGNDHDKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD 122

Query: 122 ASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFSEKYVKVGTVKSNVXXXXXXXXX 181
           ASRALAKMSF+EKDLTGDISGLGPFEL+ALQDWEYKF EKYVKVGT+K            
Sbjct: 123 ASRALAKMSFDEKDLTGDISGLGPFELDALQDWEYKFMEKYVKVGTIKKEA--TEAESTG 180

Query: 182 XXXXXXXRDVDAAKPAENGPSETAAVKSDE---NVDVNKD 218
                  RDVD   P E+G S+ AAVKS+E   NV+ +K+
Sbjct: 181 EPSGSTPRDVDVINPTEDGRSKPAAVKSEETPSNVEADKE 220


>Medtr6g027160.2 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:9196689-9192831 | 20130731
          Length = 195

 Score =  133 bits (334), Expect = 1e-31,   Method: Compositional matrix adjust.
 Identities = 69/157 (43%), Positives = 94/157 (59%), Gaps = 6/157 (3%)

Query: 18  GLSPXXXXXXXXXXXXIYYVVSGLFGSSPDHNR------HQHRHIXXXXXXXXXXXVQLG 71
           GLSP            +Y  V+ +F S  D N+                       VQ+G
Sbjct: 17  GLSPTAFFTITLLSILVYRTVTSMFVSPQDFNKPPVVSARFGSSRFNDVTEPPKKPVQVG 76

Query: 72  EITEEELKAYDGSDPAKPLLMAIKAQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 131
           EI+E+EL+ Y+GSD  KP+L+++K  IYDVSQ + FYGPGG YA+FAGK+ SRALA +SF
Sbjct: 77  EISEKELRLYNGSDENKPILISVKGNIYDVSQGKNFYGPGGSYAMFAGKECSRALALLSF 136

Query: 132 EEKDLTGDISGLGPFELEALQDWEYKFSEKYVKVGTV 168
           + +D+ G++ GL   EL  L+DWEYKF +KY KVG +
Sbjct: 137 KPQDINGNLEGLDESELAILEDWEYKFIDKYPKVGQL 173


>Medtr6g027160.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:9196455-9193480 | 20130731
          Length = 195

 Score =  133 bits (334), Expect = 1e-31,   Method: Compositional matrix adjust.
 Identities = 69/157 (43%), Positives = 94/157 (59%), Gaps = 6/157 (3%)

Query: 18  GLSPXXXXXXXXXXXXIYYVVSGLFGSSPDHNR------HQHRHIXXXXXXXXXXXVQLG 71
           GLSP            +Y  V+ +F S  D N+                       VQ+G
Sbjct: 17  GLSPTAFFTITLLSILVYRTVTSMFVSPQDFNKPPVVSARFGSSRFNDVTEPPKKPVQVG 76

Query: 72  EITEEELKAYDGSDPAKPLLMAIKAQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 131
           EI+E+EL+ Y+GSD  KP+L+++K  IYDVSQ + FYGPGG YA+FAGK+ SRALA +SF
Sbjct: 77  EISEKELRLYNGSDENKPILISVKGNIYDVSQGKNFYGPGGSYAMFAGKECSRALALLSF 136

Query: 132 EEKDLTGDISGLGPFELEALQDWEYKFSEKYVKVGTV 168
           + +D+ G++ GL   EL  L+DWEYKF +KY KVG +
Sbjct: 137 KPQDINGNLEGLDESELAILEDWEYKFIDKYPKVGQL 173


>Medtr6g027160.3 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:9196689-9192210 | 20130731
          Length = 195

 Score =  133 bits (334), Expect = 1e-31,   Method: Compositional matrix adjust.
 Identities = 69/157 (43%), Positives = 94/157 (59%), Gaps = 6/157 (3%)

Query: 18  GLSPXXXXXXXXXXXXIYYVVSGLFGSSPDHNR------HQHRHIXXXXXXXXXXXVQLG 71
           GLSP            +Y  V+ +F S  D N+                       VQ+G
Sbjct: 17  GLSPTAFFTITLLSILVYRTVTSMFVSPQDFNKPPVVSARFGSSRFNDVTEPPKKPVQVG 76

Query: 72  EITEEELKAYDGSDPAKPLLMAIKAQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 131
           EI+E+EL+ Y+GSD  KP+L+++K  IYDVSQ + FYGPGG YA+FAGK+ SRALA +SF
Sbjct: 77  EISEKELRLYNGSDENKPILISVKGNIYDVSQGKNFYGPGGSYAMFAGKECSRALALLSF 136

Query: 132 EEKDLTGDISGLGPFELEALQDWEYKFSEKYVKVGTV 168
           + +D+ G++ GL   EL  L+DWEYKF +KY KVG +
Sbjct: 137 KPQDINGNLEGLDESELAILEDWEYKFIDKYPKVGQL 173


>Medtr3g105860.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr3:48826866-48827824 | 20130731
          Length = 100

 Score =  115 bits (289), Expect = 2e-26,   Method: Compositional matrix adjust.
 Identities = 53/99 (53%), Positives = 72/99 (72%)

Query: 72  EITEEELKAYDGSDPAKPLLMAIKAQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 131
           E+T ++L  Y+G+DP+KP+ +A+K ++YDV+  + FYGPGG YA+FAGKDASRALAKMS 
Sbjct: 2   EMTAQQLSEYNGTDPSKPIYVAVKGRVYDVTTGKSFYGPGGAYAMFAGKDASRALAKMSK 61

Query: 132 EEKDLTGDISGLGPFELEALQDWEYKFSEKYVKVGTVKS 170
            E+D+T  + GL   E+  L DWE KF  KY  V T+ S
Sbjct: 62  NEEDITSSLDGLTEKEIGVLNDWETKFVAKYPVVATIVS 100


>Medtr3g081290.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr3:36822514-36819099 | 20130731
          Length = 238

 Score = 78.6 bits (192), Expect = 4e-15,   Method: Compositional matrix adjust.
 Identities = 37/96 (38%), Positives = 56/96 (58%)

Query: 73  ITEEELKAYDGSDPAKPLLMAIKAQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFE 132
            T EEL  ++G+DP  P+L+ I   ++DV++ +  YGPGG Y  FAG+DASRA    +F 
Sbjct: 35  FTTEELALFNGTDPGLPILIGILGSVFDVTKGKSHYGPGGGYHHFAGRDASRAFFSGNFT 94

Query: 133 EKDLTGDISGLGPFELEALQDWEYKFSEKYVKVGTV 168
              LT  + GL   E++++ DW   +   Y  VG +
Sbjct: 95  GDGLTDSLRGLSSTEVKSVVDWRDFYLRSYKPVGKL 130


>Medtr6g027060.1 | membrane steroid-binding protein, putative | HC |
           chr6:9163853-9164183 | 20130731
          Length = 86

 Score = 71.6 bits (174), Expect = 5e-13,   Method: Compositional matrix adjust.
 Identities = 35/77 (45%), Positives = 49/77 (63%), Gaps = 4/77 (5%)

Query: 94  IKAQIYDVSQSRM----FYGPGGPYALFAGKDASRALAKMSFEEKDLTGDISGLGPFELE 149
            K Q+  +  S +    FYG GG YA+FAGK+ +  LA +SF+ +D+ G++ G    EL 
Sbjct: 10  CKLQLTTLGDSSLYVMNFYGSGGLYAMFAGKECNLPLALLSFKPQDINGNLEGFHKSELT 69

Query: 150 ALQDWEYKFSEKYVKVG 166
            L+DWEYKF +KY KVG
Sbjct: 70  VLEDWEYKFIDKYSKVG 86