Miyakogusa Predicted Gene

Lj4g3v2742940.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2742940.1 tr|G7LE12|G7LE12_MEDTR Cysteine desulfurase
OS=Medicago truncatula GN=MTR_8g093560 PE=3 SV=1,83.7,0,no
description,Pyridoxal phosphate-dependent transferase, major region,
subdomain 1; SUBFAMILY NOT N,gene.g57379.t1.1
         (580 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g093560.1 | cysteine desulfurylase | HC | chr8:39109064-39...  1034   0.0  
Medtr8g093550.1 | cysteine desulfurylase | HC | chr8:39100008-39...   930   0.0  
Medtr3g083980.1 | SufS subfamily cysteine desulfurase | HC | chr...    65   2e-10

>Medtr8g093560.1 | cysteine desulfurylase | HC |
           chr8:39109064-39112168 | 20130731
          Length = 662

 Score = 1034 bits (2673), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 499/595 (83%), Positives = 538/595 (90%), Gaps = 20/595 (3%)

Query: 1   MGLPCNESVEEKLSWLRSQIIGHDAEFDSPFGRRKLLYADHTASGRSLHYNENFIINHLL 60
           M LPCNESVEEKL WLRSQIIG+ A+FDSPFGRRKL+YADHTASGRSL YNE+FIINHLL
Sbjct: 73  MDLPCNESVEEKLCWLRSQIIGNYAKFDSPFGRRKLVYADHTASGRSLRYNEDFIINHLL 132

Query: 61  PFYGNTHTCDSYVGSRTTKMLHEATEYIKKCLGGGEKDAIIFCGSGTTAAIKRLQEVLGI 120
           PFYGNTHTCDSYVGSRTTKMLHEATEYIKKCLGGGE DAIIFCGSGTTA+IKRLQEV+ I
Sbjct: 133 PFYGNTHTCDSYVGSRTTKMLHEATEYIKKCLGGGEDDAIIFCGSGTTASIKRLQEVMVI 192

Query: 121 AVPSIMRERLLKSLTKEERWVVFVGPHEHHSNLLSWRQSLAEVIEIGLDDQGLLDMEALK 180
           AVPSI+RE +L SL+KEERWVVFVGPHEHHSNLLSWRQSLAEV+EIGLDD+GLLDMEALK
Sbjct: 193 AVPSILRESMLNSLSKEERWVVFVGPHEHHSNLLSWRQSLAEVVEIGLDDKGLLDMEALK 252

Query: 181 LQLEAFKHSNRPLLGSFSACSNVTGIYSDTRAIARLLHQYKGFACFDFAASGPYVEIDMR 240
           LQLEA+K +NRPLLGSFSACSNVTGIYSDT AIA L H+Y G+ACFDFAASGPYVEIDMR
Sbjct: 253 LQLEAYKDTNRPLLGSFSACSNVTGIYSDTHAIAMLFHKYNGYACFDFAASGPYVEIDMR 312

Query: 241 SGQRDGYDAVFLSTHKFLGGPDSPGVLLMNKALYQLRSSPPSTCGGGTVTYVNSFNEKDT 300
           SG+ DGYDAVFLS HKFLGGPDSPGVLLMNKALY+LRSSPPSTCGGGTVTYVN+F+EKDT
Sbjct: 313 SGKNDGYDAVFLSPHKFLGGPDSPGVLLMNKALYRLRSSPPSTCGGGTVTYVNAFDEKDT 372

Query: 301 LYLENIEDRENGGTPPIIQTVRAALAFWVKEYIGYEEIEKREQHYINKALQRLVTNPNIK 360
           +Y+ENIEDRENGGTPPIIQTVRA+LAFWVKEYIGY EIEKREQ YINKAL+RLVTNPNIK
Sbjct: 373 IYMENIEDRENGGTPPIIQTVRASLAFWVKEYIGYNEIEKREQLYINKALKRLVTNPNIK 432

Query: 361 VLGNLKAKRQAILAFLIYSTTN----------ECDGDSHELNLWQEMGNQRGKPLHGPFV 410
           +LGNL AKRQAIL+FLIYSTTN          E +G   ELNLWQEMGNQRGKPLHGPFV
Sbjct: 433 ILGNLNAKRQAILSFLIYSTTNSCSSIDSSSQEHEG---ELNLWQEMGNQRGKPLHGPFV 489

Query: 411 AALLNDLFGIQARGGCACAGPYGHDLLNINKSQSLAIRSSVQEGYIGVKPGWTRVSFPYY 470
           AALLNDLFGIQARGGCACAGPYGH+LLNINKSQSLAIRS+VQEGYIGVKPGWTRVSFPYY
Sbjct: 490 AALLNDLFGIQARGGCACAGPYGHELLNINKSQSLAIRSAVQEGYIGVKPGWTRVSFPYY 549

Query: 471 MSEEDFEYILSCIEFLALYGQRFFPLYSFNLRNGSWRMKTEEFEALTKE-----GQHLLG 525
           MSE DF+YILS IEFLA+YGQRFFPLYSFNLRNGSW MKTE+FEA+ KE       HLLG
Sbjct: 550 MSESDFDYILSAIEFLAVYGQRFFPLYSFNLRNGSWTMKTEKFEAVNKEDTCNISNHLLG 609

Query: 526 TTLEKVNNDVQEGRGHIRNQSYFDAAKCVASSLPKFPPQGILQEDVDPNVLCFRV 580
             LE++N DV       RNQ+Y D AK +ASSLPKFPPQGILQE++DPN+L FRV
Sbjct: 610 KNLEEINTDVDVAVR--RNQTYLDVAKYIASSLPKFPPQGILQEEMDPNILYFRV 662


>Medtr8g093550.1 | cysteine desulfurylase | HC |
           chr8:39100008-39106346 | 20130731
          Length = 635

 Score =  930 bits (2404), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 438/589 (74%), Positives = 506/589 (85%), Gaps = 18/589 (3%)

Query: 1   MGLPCNESVEEKLSWLRSQIIGHDAEFDSPFGRRKLLYADHTASGRSLHYNENFIINHLL 60
           + LPCNESVEEKL WLRSQIIG+D EF+SPFG RKL+Y+DHTASGRSLH+ ENFI  HLL
Sbjct: 56  ISLPCNESVEEKLCWLRSQIIGNDVEFNSPFGTRKLVYSDHTASGRSLHFIENFITKHLL 115

Query: 61  PFYGNTHTCDSYVGSRTTKMLHEATEYIKKCLGGGEKDAIIFCGSGTTAAIKRLQEVLGI 120
           PFYGNTHTC+SYVG+RTTKM+HEAT+YIKKCLGGGE DA++ CGSG TAAIKRLQEV+GI
Sbjct: 116 PFYGNTHTCESYVGNRTTKMVHEATKYIKKCLGGGEDDALMLCGSGATAAIKRLQEVMGI 175

Query: 121 AVPSIMRERLLKSLTKEERWVVFVGPHEHHSNLLSWRQSLAEVIEIGLDDQGLLDMEALK 180
            VPSI+RER+L+ L++EERWVVFVGP+EHHSN+LSWRQSLAEV+EIGLDDQGLLDMEALK
Sbjct: 176 VVPSILRERMLRCLSEEERWVVFVGPYEHHSNILSWRQSLAEVVEIGLDDQGLLDMEALK 235

Query: 181 LQLEAFKHSNRPLLGSFSACSNVTGIYSDTRAIARLLHQYKGFACFDFAASGPYVEIDMR 240
           LQLEA+K +NRPLLGSFSACSNVTGIYSDTRAIARL+HQYKGFACFDFAASGPYVEIDMR
Sbjct: 236 LQLEAYKDTNRPLLGSFSACSNVTGIYSDTRAIARLIHQYKGFACFDFAASGPYVEIDMR 295

Query: 241 SGQRDGYDAVFLSTHKFLGGPDSPGVLLMNKALYQLRSSPPSTCGGGTVTYVNSFNEKDT 300
           SG+ DGYDAVF+S HKFLGGPDSPG+LLMNK LY LR SPPSTCGGGTV YVN FNEKDT
Sbjct: 296 SGKIDGYDAVFISPHKFLGGPDSPGILLMNKTLYMLRFSPPSTCGGGTVNYVNGFNEKDT 355

Query: 301 LYLENIEDRENGGTPPIIQTVRAALAFWVKEYIGYEEIEKREQHYINKALQRLVTNPNIK 360
           LYLE+IE+RENGGTPPIIQTVRAALAFWVKEYI YEEI++REQ YINKAL++ + NPNI+
Sbjct: 356 LYLEDIEERENGGTPPIIQTVRAALAFWVKEYINYEEIDRREQLYINKALKKFIPNPNIE 415

Query: 361 VLGNLKAKRQAILAFLIYSTTNECDGDSHELNLWQEMGNQRGKPLHGPFVAALLNDLFGI 420
           +LGN+  KRQAIL+F+IYST N  +         ++  N+RGK ++G FVA LLNDLFGI
Sbjct: 416 ILGNISTKRQAILSFVIYSTINRSE---------RKTKNKRGKIINGSFVATLLNDLFGI 466

Query: 421 QARGGCACAGPYGHDLLNINKSQSLAIRSSVQEGYIGVKPGWTRVSFPYYMSEEDFEYIL 480
           Q+RGGCACAGPYGHDLLNIN+SQSLA+RS++QEGY+GVKPGWTRVSFPYYM EE+FE+IL
Sbjct: 467 QSRGGCACAGPYGHDLLNINESQSLAVRSAIQEGYVGVKPGWTRVSFPYYMDEEEFEFIL 526

Query: 481 SCIEFLALYGQRFFPLYSFNLRNGSWRMKTEEFEALTKEGQHLLGTTLEKVNNDVQEGRG 540
           + IEF+++YGQRF PLY+FNL+NG+WR+K ++ E L KE       T EK NND  +  G
Sbjct: 527 TSIEFVSIYGQRFLPLYNFNLKNGNWRIKKQKLETLMKENNCYFKETKEKTNNDFSKADG 586

Query: 541 --HIRNQSY-------FDAAKCVASSLPKFPPQGILQEDVDPNVLCFRV 580
             ++R +S        F AAKC+AS LPKFP Q I   DVDPNVL F V
Sbjct: 587 EYNVRIKSVGKLRRKSFVAAKCIASRLPKFPSQSIAYPDVDPNVLHFIV 635


>Medtr3g083980.1 | SufS subfamily cysteine desulfurase | HC |
           chr3:37921733-37927979 | 20130731
          Length = 466

 Score = 65.1 bits (157), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 106/449 (23%), Positives = 173/449 (38%), Gaps = 67/449 (14%)

Query: 5   CNESVEEKLSWLRSQIIGHDAEFDSPFGRR-----KLLYADHTASGRSLHYNENFIINHL 59
            N+ V E    L S  +GH      P   +     KL+Y D+ A+ +        + ++ 
Sbjct: 42  VNQEVAEPT--LSSPSLGHTTRPHFPILHQEVNGSKLVYIDNAATSQKPAAVVKALQDYY 99

Query: 60  LPFYGNTHTCDSYVGSRTTKMLHEATEYIKKCLGGGEKDAIIFCGSGTTAAIKRLQEVLG 119
             +  N H    Y+ ++ T     A   +   +   +   I+F  + T A I  +    G
Sbjct: 100 EGYNSNVHRGIHYLSAKATDEYESARRKVAAFINASDSREIVFTRNATEA-INLVAYSWG 158

Query: 120 IAVPSIMRERLLKSLTKEERWVVFVGPHEHHSNLLSWR---QSLAEVIE-IGLDDQGLLD 175
                      L +L + +  V+ V   EHHS ++ W+   Q +  V++ + L+   + D
Sbjct: 159 -----------LSNLKQGDEIVLTVA--EHHSAIVPWQLVAQKVGAVLKFVNLNQDEIPD 205

Query: 176 MEALKLQLEAFKHSNRPLLGSFSACSNVTGIYSDTRAIARLLHQYKGFACFDFAASGPYV 235
           ++ LK  L     S +  + +    SNV       R IA   H        D   S P++
Sbjct: 206 IDKLKEVL-----SKKTKIVAVHHVSNVLASVLPIRDIAHWAHGVGAKVLVDACQSVPHM 260

Query: 236 EIDMRSGQRDGYDAVFL--STHKFLGGPDSPGVLLMNKALYQLRSSPPSTCGGGTVTYVN 293
            +D+++      DA FL  S+HK + GP   G L     L  L S PP   GG  ++ V 
Sbjct: 261 VVDVQN-----LDADFLVASSHK-MCGPTGIGFLYGKMDL--LSSMPPFLGGGEMISDV- 311

Query: 294 SFNEKDTLYLENIEDRENGGTPPIIQTVRAALAFWVKEYIGYEEIEKREQHYINKALQRL 353
            + +  T Y E    R   GTP I + +    A      IG + I + E        +RL
Sbjct: 312 -YLDHST-YAEP-PSRFEAGTPAIGEAIGLGAAIDYLSGIGMQAIHEYEVELGTYLYERL 368

Query: 354 VTNPNIKVLGNLKAKRQAILAFLIYSTTNECDGDSHELNLWQEMGNQRGKPLHGPFVAAL 413
           ++ PNI++ G   +K     +   ++  N                      LH   +A  
Sbjct: 369 LSVPNIRIYGPAPSKEVKRASLCSFNVDN----------------------LHPTDLATF 406

Query: 414 LNDLFGIQARGGCACAGPYGHDLLNINKS 442
           L+   G+  R G  CA P  H  L +N S
Sbjct: 407 LDQQHGVAIRSGHHCAQPL-HRYLGVNAS 434