Miyakogusa Predicted Gene

Lj4g3v2215480.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2215480.2 tr|G7JS28|G7JS28_MEDTR Lysine-ketoglutarate
reductase/saccharopine dehydrogenase OS=Medicago
truncat,86.84,0,Glyceraldehyde-3-phosphate dehydrogenase-like,
C-terminal domain,NULL; Formate/glycerate dehydrogena,CUFF.50522.2
         (1057 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G33150.2 | Symbols:  | lysine-ketoglutarate reductase/sacchar...  1479   0.0  
AT4G33150.1 | Symbols:  | lysine-ketoglutarate reductase/sacchar...  1479   0.0  
AT4G33150.3 | Symbols:  | lysine-ketoglutarate reductase/sacchar...   633   0.0  

>AT4G33150.2 | Symbols:  | lysine-ketoglutarate reductase/saccharopine
            dehydrogenase bifunctional enzyme |
            chr4:15985479-15991069 REVERSE LENGTH=1064
          Length = 1064

 Score = 1479 bits (3830), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 717/1055 (67%), Positives = 850/1055 (80%), Gaps = 9/1055 (0%)

Query: 2    LGNGVVGILAESVNKWERRAPLTPSHCARLLHGG---TTVSRIIVQPSTKRIFHDALYEE 58
            LGNGVVGILAE+VNKWERR PLTPSHCARLLHGG   T +SRI+VQPS KRI HDALYE+
Sbjct: 12   LGNGVVGILAETVNKWERRTPLTPSHCARLLHGGKDRTGISRIVVQPSAKRIHHDALYED 71

Query: 59   VGCEISQDLSQCGLILGIKQPKLEMILPDRAYGFFSHTHKAQSENMPLLDKILAERASLF 118
            VGCEIS DLS CGLILGIKQP+LEMILP+RAY FFSHTHKAQ ENMPLLDKIL+ER +L 
Sbjct: 72   VGCEISDDLSDCGLILGIKQPELEMILPERAYAFFSHTHKAQKENMPLLDKILSERVTLC 131

Query: 119  DYELIVGDNGKRLLAFGKFAGRAGMIDFXXXXXXXXXXXXYSTPFLSLGSSYMYPSLAAA 178
            DYELIVGD+GKRLLAFGK+AGRAG++DF            YSTPFLSLG+SYMY SLAAA
Sbjct: 132  DYELIVGDHGKRLLAFGKYAGRAGLVDFLHGLGQRYLSLGYSTPFLSLGASYMYSSLAAA 191

Query: 179  KAAVISVGEEIATQGLPVGICPLVFVFTGSGNVCSGAQEVFKLLPHTFVDPSRLCELEKM 238
            KAAVISVGEEIA+QGLP+GICPLVFVFTG+GNV  GAQE+FKLLPHTFV+PS+L EL   
Sbjct: 192  KAAVISVGEEIASQGLPLGICPLVFVFTGTGNVSLGAQEIFKLLPHTFVEPSKLPELFVK 251

Query: 239  DTNHPRH--ASKRVFQVYGCVVTAQDMVEPKDPSKVFDKTDYYAHPEHYNPIFHEKIAPY 296
            D    ++  ++KRV+QVYGC++T+QDMVE KDPSK FDK DYYAHPEHYNP+FHEKI+PY
Sbjct: 252  DKGISQNGISTKRVYQVYGCIITSQDMVEHKDPSKSFDKADYYAHPEHYNPVFHEKISPY 311

Query: 297  ASVIVNCMYWEKRFPPLLSYKQTQDLMRKGCPLVGIADITCDIGGSIEFVNRTTSIDSPF 356
             SV+VNCMYWEKRFP LLS KQ QDL +KG PLVGI DITCDIGGSIEFVNR T IDSPF
Sbjct: 312  TSVLVNCMYWEKRFPCLLSTKQLQDLTKKGLPLVGICDITCDIGGSIEFVNRATLIDSPF 371

Query: 357  FRYDPITDSYHDDMEGNGVICLAVDTLPTEFAKEASQYFGNVLSQFVISLASATDITKLP 416
            FR++P  +SY+DDM+G+GV+C+AVD LPTEFAKEASQ+FG++LS FV SLAS T+I+ LP
Sbjct: 372  FRFNPSNNSYYDDMDGDGVLCMAVDILPTEFAKEASQHFGDILSGFVGSLASMTEISDLP 431

Query: 417  AHLRRACITHGGVLTSLYDYIPRMRXXX-XXXXXXXXXXXLTNKSKYNISVSLSGHLFDK 475
            AHL+RACI++ G LTSLY+YIPRMR               ++++  +NI VSLSGHLFDK
Sbjct: 432  AHLKRACISYRGELTSLYEYIPRMRKSNPEEAQDNIIANGVSSQRTFNILVSLSGHLFDK 491

Query: 476  FLINEALDIIEAAGGSFHLVNCRVGQSVDAISYSELEVGADDRAVLDHIIDSLTNLANPT 535
            FLINEALD+IEAAGGSFHL  C +GQS DA SYSELEVGADD+ VLD IIDSLT LANP 
Sbjct: 492  FLINEALDMIEAAGGSFHLAKCELGQSADAESYSELEVGADDKRVLDQIIDSLTRLANPN 551

Query: 536  ENYNSSNQDLNKISLKLGKVQ-ENGIEKETDSKKKAAVLILGAGRVCQPAAEMLSSFGRL 594
            E+Y S +++ NKISLK+GKVQ EN I+++ +  KK+ VLILGAGRVC+PAA+ L+S   +
Sbjct: 552  EDYISPHREANKISLKIGKVQQENEIKEKPEMTKKSGVLILGAGRVCRPAADFLASVRTI 611

Query: 595  SSSQWYNTLLXXXXXXXXXXXXXXGSLYLKDAEQIVDGIPNVTGVQLDVMDHANLFKYIS 654
            SS QWY T                 SLYLKDA++ V+GI +V  V+LDV D  +L KY+S
Sbjct: 612  SSQQWYKTYFGADSEEKTDVHVIVASLYLKDAKETVEGISDVEAVRLDVSDSESLLKYVS 671

Query: 655  QVDVVISLLPASCHIVVANACIELKKHLVTASYVDSSMSMLDDKAKYAGVTILGEMGLDP 714
            QVDVV+SLLPASCH VVA  CIELKKHLVTASYVD   SML +KAK AG+TILGEMGLDP
Sbjct: 672  QVDVVLSLLPASCHAVVAKTCIELKKHLVTASYVDDETSMLHEKAKSAGITILGEMGLDP 731

Query: 715  GIDHMMAMKMINHARMQKGKIKSFTSYCGGLPSPEAADNPLGYKFSWNPIGAIRAGRNPA 774
            GIDHMMAMKMIN A ++KGK+KSFTSYCGGLPSP AA+NPL YKFSWNP GAIRAG+NPA
Sbjct: 732  GIDHMMAMKMINDAHIKKGKVKSFTSYCGGLPSPAAANNPLAYKFSWNPAGAIRAGQNPA 791

Query: 775  TYKYHGETVHVAGENLYDSATRLRIPDFPAFALECLPNRDSLVYGDLYGIGSEASTIFRG 834
             YK +G+ +HV G+NLYDSA R R+P+ PAFALEC PNRDSLVYG+ YGI SEA+TIFRG
Sbjct: 792  KYKSNGDIIHVDGKNLYDSAARFRVPNLPAFALECFPNRDSLVYGEHYGIESEATTIFRG 851

Query: 835  TLRYEGFGEIMGSLSRIGLFNNEAHPILKNEPRPTFRKFMFELLKVASDHPDGPLMEEED 894
            TLRYEGF  IM +LS++G F++EA+ +L    R TF   +  +L   +D+   PL  EE+
Sbjct: 852  TLRYEGFSMIMATLSKLGFFDSEANQVLSTGKRITFGALLSNILNKDADNESEPLAGEEE 911

Query: 895  ITERILKLGHCRDQISAIKTANTIIFLGLLEQTEIPASCQSAFDAVCLRMEKRLSYSNTE 954
            I++RI+KLGH ++  +A K A TI+FLG  E+ E+P+ C+S FDA C  ME++L+YS  E
Sbjct: 912  ISKRIIKLGHSKE--TAAKAAKTIVFLGFNEEREVPSLCKSVFDATCYLMEEKLAYSGNE 969

Query: 955  KDMVLLYHEVEIEYPDSQITEKHRATLLEFGKTHDGKTTSAMALTVGIPAAIGAXXXXTN 1014
            +DMVLL+HEVE+E+ +S+  EKH ATLLEFG   +G+TT+AMA TVGIPAAIGA     +
Sbjct: 970  QDMVLLHHEVEVEFLESKRIEKHTATLLEFGDIKNGQTTTAMAKTVGIPAAIGALLLIED 1029

Query: 1015 KIQTRGVLRPIVPEVYTPALDIIQAYGIKLIEKNE 1049
            KI+TRGVLRP+  EVY PALDI+QAYGIKL+EK E
Sbjct: 1030 KIKTRGVLRPLEAEVYLPALDILQAYGIKLMEKAE 1064


>AT4G33150.1 | Symbols:  | lysine-ketoglutarate reductase/saccharopine
            dehydrogenase bifunctional enzyme |
            chr4:15985479-15991069 REVERSE LENGTH=1064
          Length = 1064

 Score = 1479 bits (3830), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 717/1055 (67%), Positives = 850/1055 (80%), Gaps = 9/1055 (0%)

Query: 2    LGNGVVGILAESVNKWERRAPLTPSHCARLLHGG---TTVSRIIVQPSTKRIFHDALYEE 58
            LGNGVVGILAE+VNKWERR PLTPSHCARLLHGG   T +SRI+VQPS KRI HDALYE+
Sbjct: 12   LGNGVVGILAETVNKWERRTPLTPSHCARLLHGGKDRTGISRIVVQPSAKRIHHDALYED 71

Query: 59   VGCEISQDLSQCGLILGIKQPKLEMILPDRAYGFFSHTHKAQSENMPLLDKILAERASLF 118
            VGCEIS DLS CGLILGIKQP+LEMILP+RAY FFSHTHKAQ ENMPLLDKIL+ER +L 
Sbjct: 72   VGCEISDDLSDCGLILGIKQPELEMILPERAYAFFSHTHKAQKENMPLLDKILSERVTLC 131

Query: 119  DYELIVGDNGKRLLAFGKFAGRAGMIDFXXXXXXXXXXXXYSTPFLSLGSSYMYPSLAAA 178
            DYELIVGD+GKRLLAFGK+AGRAG++DF            YSTPFLSLG+SYMY SLAAA
Sbjct: 132  DYELIVGDHGKRLLAFGKYAGRAGLVDFLHGLGQRYLSLGYSTPFLSLGASYMYSSLAAA 191

Query: 179  KAAVISVGEEIATQGLPVGICPLVFVFTGSGNVCSGAQEVFKLLPHTFVDPSRLCELEKM 238
            KAAVISVGEEIA+QGLP+GICPLVFVFTG+GNV  GAQE+FKLLPHTFV+PS+L EL   
Sbjct: 192  KAAVISVGEEIASQGLPLGICPLVFVFTGTGNVSLGAQEIFKLLPHTFVEPSKLPELFVK 251

Query: 239  DTNHPRH--ASKRVFQVYGCVVTAQDMVEPKDPSKVFDKTDYYAHPEHYNPIFHEKIAPY 296
            D    ++  ++KRV+QVYGC++T+QDMVE KDPSK FDK DYYAHPEHYNP+FHEKI+PY
Sbjct: 252  DKGISQNGISTKRVYQVYGCIITSQDMVEHKDPSKSFDKADYYAHPEHYNPVFHEKISPY 311

Query: 297  ASVIVNCMYWEKRFPPLLSYKQTQDLMRKGCPLVGIADITCDIGGSIEFVNRTTSIDSPF 356
             SV+VNCMYWEKRFP LLS KQ QDL +KG PLVGI DITCDIGGSIEFVNR T IDSPF
Sbjct: 312  TSVLVNCMYWEKRFPCLLSTKQLQDLTKKGLPLVGICDITCDIGGSIEFVNRATLIDSPF 371

Query: 357  FRYDPITDSYHDDMEGNGVICLAVDTLPTEFAKEASQYFGNVLSQFVISLASATDITKLP 416
            FR++P  +SY+DDM+G+GV+C+AVD LPTEFAKEASQ+FG++LS FV SLAS T+I+ LP
Sbjct: 372  FRFNPSNNSYYDDMDGDGVLCMAVDILPTEFAKEASQHFGDILSGFVGSLASMTEISDLP 431

Query: 417  AHLRRACITHGGVLTSLYDYIPRMRXXX-XXXXXXXXXXXLTNKSKYNISVSLSGHLFDK 475
            AHL+RACI++ G LTSLY+YIPRMR               ++++  +NI VSLSGHLFDK
Sbjct: 432  AHLKRACISYRGELTSLYEYIPRMRKSNPEEAQDNIIANGVSSQRTFNILVSLSGHLFDK 491

Query: 476  FLINEALDIIEAAGGSFHLVNCRVGQSVDAISYSELEVGADDRAVLDHIIDSLTNLANPT 535
            FLINEALD+IEAAGGSFHL  C +GQS DA SYSELEVGADD+ VLD IIDSLT LANP 
Sbjct: 492  FLINEALDMIEAAGGSFHLAKCELGQSADAESYSELEVGADDKRVLDQIIDSLTRLANPN 551

Query: 536  ENYNSSNQDLNKISLKLGKVQ-ENGIEKETDSKKKAAVLILGAGRVCQPAAEMLSSFGRL 594
            E+Y S +++ NKISLK+GKVQ EN I+++ +  KK+ VLILGAGRVC+PAA+ L+S   +
Sbjct: 552  EDYISPHREANKISLKIGKVQQENEIKEKPEMTKKSGVLILGAGRVCRPAADFLASVRTI 611

Query: 595  SSSQWYNTLLXXXXXXXXXXXXXXGSLYLKDAEQIVDGIPNVTGVQLDVMDHANLFKYIS 654
            SS QWY T                 SLYLKDA++ V+GI +V  V+LDV D  +L KY+S
Sbjct: 612  SSQQWYKTYFGADSEEKTDVHVIVASLYLKDAKETVEGISDVEAVRLDVSDSESLLKYVS 671

Query: 655  QVDVVISLLPASCHIVVANACIELKKHLVTASYVDSSMSMLDDKAKYAGVTILGEMGLDP 714
            QVDVV+SLLPASCH VVA  CIELKKHLVTASYVD   SML +KAK AG+TILGEMGLDP
Sbjct: 672  QVDVVLSLLPASCHAVVAKTCIELKKHLVTASYVDDETSMLHEKAKSAGITILGEMGLDP 731

Query: 715  GIDHMMAMKMINHARMQKGKIKSFTSYCGGLPSPEAADNPLGYKFSWNPIGAIRAGRNPA 774
            GIDHMMAMKMIN A ++KGK+KSFTSYCGGLPSP AA+NPL YKFSWNP GAIRAG+NPA
Sbjct: 732  GIDHMMAMKMINDAHIKKGKVKSFTSYCGGLPSPAAANNPLAYKFSWNPAGAIRAGQNPA 791

Query: 775  TYKYHGETVHVAGENLYDSATRLRIPDFPAFALECLPNRDSLVYGDLYGIGSEASTIFRG 834
             YK +G+ +HV G+NLYDSA R R+P+ PAFALEC PNRDSLVYG+ YGI SEA+TIFRG
Sbjct: 792  KYKSNGDIIHVDGKNLYDSAARFRVPNLPAFALECFPNRDSLVYGEHYGIESEATTIFRG 851

Query: 835  TLRYEGFGEIMGSLSRIGLFNNEAHPILKNEPRPTFRKFMFELLKVASDHPDGPLMEEED 894
            TLRYEGF  IM +LS++G F++EA+ +L    R TF   +  +L   +D+   PL  EE+
Sbjct: 852  TLRYEGFSMIMATLSKLGFFDSEANQVLSTGKRITFGALLSNILNKDADNESEPLAGEEE 911

Query: 895  ITERILKLGHCRDQISAIKTANTIIFLGLLEQTEIPASCQSAFDAVCLRMEKRLSYSNTE 954
            I++RI+KLGH ++  +A K A TI+FLG  E+ E+P+ C+S FDA C  ME++L+YS  E
Sbjct: 912  ISKRIIKLGHSKE--TAAKAAKTIVFLGFNEEREVPSLCKSVFDATCYLMEEKLAYSGNE 969

Query: 955  KDMVLLYHEVEIEYPDSQITEKHRATLLEFGKTHDGKTTSAMALTVGIPAAIGAXXXXTN 1014
            +DMVLL+HEVE+E+ +S+  EKH ATLLEFG   +G+TT+AMA TVGIPAAIGA     +
Sbjct: 970  QDMVLLHHEVEVEFLESKRIEKHTATLLEFGDIKNGQTTTAMAKTVGIPAAIGALLLIED 1029

Query: 1015 KIQTRGVLRPIVPEVYTPALDIIQAYGIKLIEKNE 1049
            KI+TRGVLRP+  EVY PALDI+QAYGIKL+EK E
Sbjct: 1030 KIKTRGVLRPLEAEVYLPALDILQAYGIKLMEKAE 1064


>AT4G33150.3 | Symbols:  | lysine-ketoglutarate reductase/saccharopine
            dehydrogenase bifunctional enzyme |
            chr4:15985479-15988055 REVERSE LENGTH=482
          Length = 482

 Score =  633 bits (1633), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 303/482 (62%), Positives = 371/482 (76%), Gaps = 2/482 (0%)

Query: 568  KKAAVLILGAGRVCQPAAEMLSSFGRLSSSQWYNTLLXXXXXXXXXXXXXXGSLYLKDAE 627
            KK+ VLILGAGRVC+PAA+ L+S   +SS QWY T                 SLYLKDA+
Sbjct: 3    KKSGVLILGAGRVCRPAADFLASVRTISSQQWYKTYFGADSEEKTDVHVIVASLYLKDAK 62

Query: 628  QIVDGIPNVTGVQLDVMDHANLFKYISQVDVVISLLPASCHIVVANACIELKKHLVTASY 687
            + V+GI +V  V+LDV D  +L KY+SQVDVV+SLLPASCH VVA  CIELKKHLVTASY
Sbjct: 63   ETVEGISDVEAVRLDVSDSESLLKYVSQVDVVLSLLPASCHAVVAKTCIELKKHLVTASY 122

Query: 688  VDSSMSMLDDKAKYAGVTILGEMGLDPGIDHMMAMKMINHARMQKGKIKSFTSYCGGLPS 747
            VD   SML +KAK AG+TILGEMGLDPGIDHMMAMKMIN A ++KGK+KSFTSYCGGLPS
Sbjct: 123  VDDETSMLHEKAKSAGITILGEMGLDPGIDHMMAMKMINDAHIKKGKVKSFTSYCGGLPS 182

Query: 748  PEAADNPLGYKFSWNPIGAIRAGRNPATYKYHGETVHVAGENLYDSATRLRIPDFPAFAL 807
            P AA+NPL YKFSWNP GAIRAG+NPA YK +G+ +HV G+NLYDSA R R+P+ PAFAL
Sbjct: 183  PAAANNPLAYKFSWNPAGAIRAGQNPAKYKSNGDIIHVDGKNLYDSAARFRVPNLPAFAL 242

Query: 808  ECLPNRDSLVYGDLYGIGSEASTIFRGTLRYEGFGEIMGSLSRIGLFNNEAHPILKNEPR 867
            EC PNRDSLVYG+ YGI SEA+TIFRGTLRYEGF  IM +LS++G F++EA+ +L    R
Sbjct: 243  ECFPNRDSLVYGEHYGIESEATTIFRGTLRYEGFSMIMATLSKLGFFDSEANQVLSTGKR 302

Query: 868  PTFRKFMFELLKVASDHPDGPLMEEEDITERILKLGHCRDQISAIKTANTIIFLGLLEQT 927
             TF   +  +L   +D+   PL  EE+I++RI+KLGH ++  +A K A TI+FLG  E+ 
Sbjct: 303  ITFGALLSNILNKDADNESEPLAGEEEISKRIIKLGHSKE--TAAKAAKTIVFLGFNEER 360

Query: 928  EIPASCQSAFDAVCLRMEKRLSYSNTEKDMVLLYHEVEIEYPDSQITEKHRATLLEFGKT 987
            E+P+ C+S FDA C  ME++L+YS  E+DMVLL+HEVE+E+ +S+  EKH ATLLEFG  
Sbjct: 361  EVPSLCKSVFDATCYLMEEKLAYSGNEQDMVLLHHEVEVEFLESKRIEKHTATLLEFGDI 420

Query: 988  HDGKTTSAMALTVGIPAAIGAXXXXTNKIQTRGVLRPIVPEVYTPALDIIQAYGIKLIEK 1047
             +G+TT+AMA TVGIPAAIGA     +KI+TRGVLRP+  EVY PALDI+QAYGIKL+EK
Sbjct: 421  KNGQTTTAMAKTVGIPAAIGALLLIEDKIKTRGVLRPLEAEVYLPALDILQAYGIKLMEK 480

Query: 1048 NE 1049
             E
Sbjct: 481  AE 482