Miyakogusa Predicted Gene

Lj2g3v3336140.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v3336140.1 tr|G7K4V4|G7K4V4_MEDTR Neutral ceramidase
OS=Medicago truncatula GN=MTR_5g096890 PE=4 SV=1,83.36,0,seg,NULL;
Ceramidase_alk,Neutral/alkaline nonlysosomal ceramidase;
CERAMIDASE,Neutral/alkaline nonly,CUFF.40033.1
         (681 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G07380.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramid...   902   0.0  
AT2G38010.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramid...   859   0.0  
AT2G38010.2 | Symbols:  | Neutral/alkaline non-lysosomal ceramid...   843   0.0  
AT5G58980.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramid...   803   0.0  

>AT1G07380.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramidase
           | chr1:2264829-2268306 REVERSE LENGTH=779
          Length = 779

 Score =  902 bits (2330), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 438/615 (71%), Positives = 485/615 (78%), Gaps = 17/615 (2%)

Query: 79  MELPSLSHWRATATMRVWPFFL----LLILLKSDVVYSDSNYLIGLGSYDITGPAADVNM 134
           MEL  +  W        +P F     +L LL    VYS S YLIGLGSYDITGPAADVNM
Sbjct: 1   MELSLVRLWNT-----CFPSFFYISTVLFLLTGGGVYSHSEYLIGLGSYDITGPAADVNM 55

Query: 135 MGYTNTEQIASGVHFRLRARTFIAAEPKGNRAAFVNLDACMASDLITTKVFERLKARYGD 194
           MGY N EQ+ASG+HFRLRARTFI +EP+G R  FVNLDACMAS ++  KV ERLKARYGD
Sbjct: 56  MGYANMEQVASGIHFRLRARTFIVSEPQGKRVVFVNLDACMASQIVKLKVIERLKARYGD 115

Query: 195 LYTEKNVAISGTHTHAGPGGFLQYVTYLVTSLGFVRQSFDVIVDGIEKSIIQAHENLRPG 254
           LYTE+NV ISG HTHAGPGG+LQYV Y+VTSLGFVRQSFD +VDGIE SIIQAHENLRPG
Sbjct: 116 LYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPG 175

Query: 255 SIFVNKGELLDAGVNRSPSAYLNNPAAERSKYKYNVDKEMTLLKFVDDEWGPSGSFNWFA 314
           SIF+N GELLDAGVNRSPSAYLNNP+ ERSK+KYNVDKEMTLLKFVDD+WGP GSFNWFA
Sbjct: 176 SIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEMTLLKFVDDQWGPVGSFNWFA 235

Query: 315 THGTSMSRTNSLISGDNKGAAARFMEDWFEKKG-----SAGFENDGLPRRISNIIPSXXX 369
           THGTSMSRTNSLISGDNKGAA+RFMEDW+E+       S  F +D +PRR+S++I +   
Sbjct: 236 THGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQD 295

Query: 370 XXXXXXXXXXSFQSPPGRPATRTSSVARRVRGVLKQVDKPRFVSAFCQSNCGDVSPNTLG 429
                      F+S PG+P TR SS ARRVR  L++ DKP FVSAFCQ+NCGDVSPN LG
Sbjct: 296 SHHELLELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLG 355

Query: 430 AFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTHTIGERQFRKAVELFNGASEQV 489
           AFCLDTGLPCDFNHSTCGGKNE+CYGRGPGYPDEFEST  IGERQF+ A+ELFN ASEQ+
Sbjct: 356 AFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQL 415

Query: 490 KGKVDFRHAYIDFSQLEVNLSSA-GASKGAKTCPXXXXXXXXXXXXDGPGAFDFKQGDVK 548
           +GKVD+RH Y+DFSQL V L    G S+  KTCP            DGPGAFDF QGD K
Sbjct: 416 QGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDK 475

Query: 549 GNPFWNTVRNLLKKPGKEQIDCQYPKPILLDIGEMKIPYDWAPSILPIQILRIGQFVILS 608
           GNPFW  VRN+LK P K+QIDC YPKPILLD GEM  PYDWAPSIL +Q+LRIGQ  ILS
Sbjct: 476 GNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILS 535

Query: 609 VPGEFTTMAGRRLRDAVKEVL--SADKDFGRNIHVVLAGLTNTYSQYVTTYEEYEVQRYE 666
           VPGEFTTMAGRRLR AVK  L  S +KD    IHVV+AGL N YSQYVTT+EEY+VQRYE
Sbjct: 536 VPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYE 595

Query: 667 GASTLYGPHTLSAYI 681
           GASTLYGPHTLS YI
Sbjct: 596 GASTLYGPHTLSGYI 610


>AT2G38010.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramidase
           | chr2:15906862-15909867 FORWARD LENGTH=757
          Length = 757

 Score =  859 bits (2220), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 408/569 (71%), Positives = 466/569 (81%), Gaps = 7/569 (1%)

Query: 116 YLIGLGSYDITGPAADVNMMGYTNTEQIASGVHFRLRARTFIAAEPKGNRAAFVNLDACM 175
           YLIG+GSYDITGPAADVNMMGY N++QIASG+HFRLRAR FI AEP+GNR  FVNLDACM
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEPQGNRVVFVNLDACM 85

Query: 176 ASDLITTKVFERLKARYGDLYTEKNVAISGTHTHAGPGGFLQYVTYLVTSLGFVRQSFDV 235
           AS ++T KV ERLKARYG+LYTEKNVAISG HTHAGPGG+LQYVTY+VTSLGFVRQSFDV
Sbjct: 86  ASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDV 145

Query: 236 IVDGIEKSIIQAHENLRPGSIFVNKGELLDAGVNRSPSAYLNNPAAERSKYKYNVDKEMT 295
           +V+GIE+SI+QAHE+LRPGS FVNKG+LLDAGVNRSPS+YLNNPAAERSKYKY+VDKEMT
Sbjct: 146 VVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMT 205

Query: 296 LLKFVDDEWGPSGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGSAGFENDG 355
           L+KFVD + GP+GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFE        +  
Sbjct: 206 LVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRN 265

Query: 356 LPRRISNIIPSXXXXXXXXXXXXXSFQSPPGRPATRTSSVARRVRGVLKQVDKPRFVSAF 415
           +PRR+S I+               +++S  G    ++  V  RVR   K+    +FVSAF
Sbjct: 266 IPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKR----KFVSAF 321

Query: 416 CQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTHTIGERQF 475
           CQSNCGDVSPNTLG FC+DTGLPCDFNHSTC G+NELCYGRGPGYPDEFEST  IGE+QF
Sbjct: 322 CQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQF 381

Query: 476 RKAVELFNGASEQVKGKVDFRHAYIDFSQLEVNLSSA-GASKGAKTCPXXXXXXXXXXXX 534
           + AVELFN A+E+++GK+ ++HAY+DFS L+V +  A G S+  KTCP            
Sbjct: 382 KMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTT 441

Query: 535 DGPGAFDFKQGDVKGNPFWNTVRNLLKKPGKEQIDCQYPKPILLDIGEMKIPYDWAPSIL 594
           DGPGAFDFKQGD +GN FW  VRN+L+ PG EQ+ CQ PKPILLD GEMK PYDWAPSIL
Sbjct: 442 DGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSIL 501

Query: 595 PIQILRIGQFVILSVPGEFTTMAGRRLRDAVKEVL--SADKDFGRNIHVVLAGLTNTYSQ 652
           PIQILRIGQ VILSVPGEFTTMAGRRLRDA+K  L  S  K+F  N+HVV+AGLTNTYSQ
Sbjct: 502 PIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQ 561

Query: 653 YVTTYEEYEVQRYEGASTLYGPHTLSAYI 681
           Y+ T+EEYEVQRYEGASTLYG HTL+AYI
Sbjct: 562 YIATFEEYEVQRYEGASTLYGRHTLTAYI 590


>AT2G38010.2 | Symbols:  | Neutral/alkaline non-lysosomal ceramidase
           | chr2:15906862-15909867 FORWARD LENGTH=792
          Length = 792

 Score =  843 bits (2179), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 408/604 (67%), Positives = 466/604 (77%), Gaps = 42/604 (6%)

Query: 116 YLIGLGSYDITGPAADVNMMGYTNTEQIASGVHFRLRARTFIAAEPKGNRAAFVNLDACM 175
           YLIG+GSYDITGPAADVNMMGY N++QIASG+HFRLRAR FI AEP+GNR  FVNLDACM
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEPQGNRVVFVNLDACM 85

Query: 176 ASDLITTKVFERLKARYGDLYTEKNVAISGTHTHAGPGGFLQYVTYLVTSLGFVRQSFDV 235
           AS ++T KV ERLKARYG+LYTEKNVAISG HTHAGPGG+LQYVTY+VTSLGFVRQSFDV
Sbjct: 86  ASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDV 145

Query: 236 IVDGIEKSIIQAHENLRPGSIFVNKGELLDAGVNRSPSAYLNNPAAERSKYKYNVDKEMT 295
           +V+GIE+SI+QAHE+LRPGS FVNKG+LLDAGVNRSPS+YLNNPAAERSKYKY+VDKEMT
Sbjct: 146 VVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMT 205

Query: 296 LLKFVDDEWGPSGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGSAGFENDG 355
           L+KFVD + GP+GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFE        +  
Sbjct: 206 LVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRN 265

Query: 356 LPRRISNIIPSXX-----------------------------------XXXXXXXXXXXS 380
           +PRR+S I+                                                  +
Sbjct: 266 IPRRVSTIVSDFSRNRESYPFLELPNVAILYIAVAERLMWKVMVLFYFVSESRLLDIAAT 325

Query: 381 FQSPPGRPATRTSSVARRVRGVLKQVDKPRFVSAFCQSNCGDVSPNTLGAFCLDTGLPCD 440
           ++S  G    ++  V  RVR   K+    +FVSAFCQSNCGDVSPNTLG FC+DTGLPCD
Sbjct: 326 YKSSRGHSVDKSLDVKTRVRNGSKR----KFVSAFCQSNCGDVSPNTLGTFCIDTGLPCD 381

Query: 441 FNHSTCGGKNELCYGRGPGYPDEFESTHTIGERQFRKAVELFNGASEQVKGKVDFRHAYI 500
           FNHSTC G+NELCYGRGPGYPDEFEST  IGE+QF+ AVELFN A+E+++GK+ ++HAY+
Sbjct: 382 FNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYL 441

Query: 501 DFSQLEVNLSSA-GASKGAKTCPXXXXXXXXXXXXDGPGAFDFKQGDVKGNPFWNTVRNL 559
           DFS L+V +  A G S+  KTCP            DGPGAFDFKQGD +GN FW  VRN+
Sbjct: 442 DFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNV 501

Query: 560 LKKPGKEQIDCQYPKPILLDIGEMKIPYDWAPSILPIQILRIGQFVILSVPGEFTTMAGR 619
           L+ PG EQ+ CQ PKPILLD GEMK PYDWAPSILPIQILRIGQ VILSVPGEFTTMAGR
Sbjct: 502 LRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGR 561

Query: 620 RLRDAVKEVL--SADKDFGRNIHVVLAGLTNTYSQYVTTYEEYEVQRYEGASTLYGPHTL 677
           RLRDA+K  L  S  K+F  N+HVV+AGLTNTYSQY+ T+EEYEVQRYEGASTLYG HTL
Sbjct: 562 RLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTL 621

Query: 678 SAYI 681
           +AYI
Sbjct: 622 TAYI 625


>AT5G58980.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramidase
           | chr5:23811449-23814395 REVERSE LENGTH=733
          Length = 733

 Score =  803 bits (2075), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 392/573 (68%), Positives = 440/573 (76%), Gaps = 32/573 (5%)

Query: 110 VYSDSNYLIGLGSYDITGPAADVNMMGYTNTEQIASGVHFRLRARTFIAAEPKGNRAAFV 169
           ++SDS+YL+GLGSYDITGPAADVNMMGY N EQ+ASGVHFRLRAR FI AEP   R AFV
Sbjct: 23  IFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYKKRIAFV 82

Query: 170 NLDACMASDLITTKVFERLKARYGDLYTEKNVAISGTHTHAGPGGFLQYVTYLVTSLGFV 229
           NLDA MAS L+T KV ERLK RYG+LYTE+NVAISGTHTHAGPGG+LQY+ YLVTSLGFV
Sbjct: 83  NLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFV 142

Query: 230 RQSFDVIVDGIEKSIIQAHENLRPGSIFVNKGELLDAGVNRSPSAYLNNPAAERSKYKYN 289
            QSF+ +VDGIE+SIIQAHENLRPGSI +NKGELLDAGVNRSPSAYLNNPA ERSKY+Y+
Sbjct: 143 HQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYD 202

Query: 290 VDKEMTLLKFVDDEWGPSGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEKKGSA 349
           VDKEMTL+KFVDD+WGP                             AR MEDWFE++   
Sbjct: 203 VDKEMTLVKFVDDQWGP----------------------------VARIMEDWFERENGC 234

Query: 350 GFENDGLPRRISNIIPSXXXXXXXXXXXXXSFQSPPGRPATRTSSVARRVRGVLKQVDKP 409
              +   PRR+S+II               S  S  G+  TR SSVARRVR   +  DKP
Sbjct: 235 RSVDVESPRRVSSIISDPYDQDLMEMAS--SLLSTGGKTVTRMSSVARRVRSRFRHADKP 292

Query: 410 RFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTHT 469
           RFVSAFCQ+NCGDVSPN LGAFC+DTGLPC+FN STCGGKNE CYGRGPGYPDEFEST  
Sbjct: 293 RFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRI 352

Query: 470 IGERQFRKAVELFNGASEQVKGKVDFRHAYIDFSQLEVNLSSA-GASKGAKTCPXXXXXX 528
           IGERQF+KA +LF  ASE+++GKVD+RHAY+DFSQLEV ++   G S+  KTCP      
Sbjct: 353 IGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFG 412

Query: 529 XXXXXXDGPGAFDFKQGDVKGNPFWNTVRNLLKKPGKEQIDCQYPKPILLDIGEMKIPYD 588
                 DGPGAFDFKQGD +GNPFW  VRNLLK P +EQ+ CQ PKPILLD GEMK PYD
Sbjct: 413 FAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYD 472

Query: 589 WAPSILPIQILRIGQFVILSVPGEFTTMAGRRLRDAVKEVLSADKDFGRNIHVVLAGLTN 648
           WAPSILP+QILRIGQ VIL VPGEFTTMAGRRLRDAVK VL    + GR   VV+AGLTN
Sbjct: 473 WAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSN-GREFSVVIAGLTN 531

Query: 649 TYSQYVTTYEEYEVQRYEGASTLYGPHTLSAYI 681
           +YSQY+ T+EEY+VQRYEGASTLYGPHTLS YI
Sbjct: 532 SYSQYIATFEEYQVQRYEGASTLYGPHTLSGYI 564