Restriction map for pF1KE0140
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BstBI       
BstEII      BstPI       BstXI       BstYI       Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
SbfI        SexAI       SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


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# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccI          472  GTATAC               GT^[AC][GT]AC
AccII          23  CGCG                 CG^CG
AfaI          633  GTAC                 GT^AC
AflII         492  CTTAAG               C^TTAAG
AgeI          344  ACCGGT               A^CCGGT
AlwI          669  GGATC                GGATC(4/5)
AlwNI         512  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       821  GAAACCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         730  GAGCAC               G[AT]GC[AT]^C
AvaI           59  CCCGAG               C^[CT]CG[GA]G
BanII         139  GAGCCC               G[GA]GC[CT]^C
BfrI          492  CTTAAG               C^TTAAG
BglI          551  GCCTGCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI          22  CCGCGG               C^C[ACGT][ACGT]GG
BsaWI         344  ACCGGT               [AT]^CCGG[AT]
BseRI         106  GAGGAG               GAGGAG(10/8)
BsgI          365  CTGCAC               GTGCAG(16/14)
BsiHKAI       730  GAGCAC               G[AT]GC[AT]^C
BsmAI         643  GAGAC                GTCTC(1/5)
BsoBI          59  CCCGAG               C^[CT]CG[GA]G
BsrI          696  ACTGG                ACTGG(1/-1)
BssSI         383  CACGAG               CACGAG(-5/-1)
Bst1107I      472  GTATAC               GTA^TAC
BstAPI        367  GCACATCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        160  GGATG                GGATG(2/0)
BstUI          23  CGCG                 CG^CG
BstZ17I       472  GTATAC               GTA^TAC
CfoI          408  GCGC                 GCG^C
DpnII         670  GATC                 ^GATC
DraII         215  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        368  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           22  CCGCGG               C^C[GA][CT]GG
EarI          700  GAAGAG               CTCTTC(1/4)
EcoNI         601  CCTGTAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      215  AGGACCT              [GA]G^G[ACGT]CC[CT]
FokI          160  GGATG                GGATG(9/13)
HaeII         407  GGCGCT               [GA]GCGC^[CT]
HgaI          111  GCGTC                GACGC(5/10)
HhaI          408  GCGC                 GCG^C
HinP1I        408  GCGC                 G^CGC
HphI          640  GGTGA                GGTGA(8/7)
Ksp632I       700  GAAGAG               CTCTTC(1/4)
KspI           22  CCGCGG               CCGC^GG
MboI          670  GATC                 ^GATC
MseI          493  TTAA                 T^TAA
MspA1I         22  CCGCGG               C[AC]G^C[GT]G
MvnI           23  CGCG                 CG^CG
NdeII         670  GATC                 ^GATC
PinAI         344  ACCGGT               A^CCGGT
PleI           62  GAGTC                GAGTC(4/5)
PpuMI         215  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          425  CTGCAG               CTGCA^G
RsaI          633  GTAC                 GT^AC
SacII          22  CCGCGG               CCGC^GG
Sau3AI        670  GATC                 ^GATC
ScaI          632  AGTACT               AGT^ACT
SfiI          550  GGCCTGCGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI          460  ACTAGT               A^CTAGT
TaqI          672  TCGA                 T^CGA
Tru9I         493  TTAA                 T^TAA
TthHB8I       672  TCGA                 T^CGA
XmnI          821  GAAACCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          206  GAATTT               [GA]^AATT[CT]
              332  AAATTT              
ApoI          206  GAATTT               [GA]^AATT[CT]
              332  AAATTT              
AvaII         165  GGACC                G^G[AT]CC
              216  GGACC               
BanI            6  GGTGCC               G^G[CT][GA]CC
               18  GGCACC              
BbvI           70  GCTGC                GCAGC(8/12)
              376  GCTGC               
BcnI          150  CCCGG                CC^[GC]GG
              174  CCGGG               
BfaI          461  CTAG                 C^TAG
              818  CTAG                
BsmFI         164  GGGAC                GGGAC(10/14)
              133  GTCCC               
BsrFI          15  ACCGGC               [GA]^CCGG[CT]
              344  ACCGGT              
BstNI         324  CCAGG                CC^[AT]GG
              561  CCTGG               
Cfr10I         15  ACCGGC               [GA]^CCGG[CT]
              344  ACCGGT              
Cfr13I        165  GGACC                G^G[ACGT]CC
              216  GGACC               
EcoRII        324  CCAGG                ^CC[AT]GG
              561  CCTGG               
MaeI          461  CTAG                 C^TAG
              818  CTAG                
MaeII         131  ACGT                 A^CGT
              545  ACGT                
MboII         700  GAAGA                GAAGA(8/7)
              766  GAAGA               
MvaI          324  CCAGG                CC^[AT]GG
              561  CCTGG               
NciI          150  CCCGG                CC^[GC]GG
              174  CCGGG               
NlaIII        295  CATG                 CATG^
              685  CATG                
Sau96I        165  GGACC                G^G[ACGT]CC
              216  GGACC               
SmlI          103  CTTGAG               C^T[CT][GA]AG
              492  CTTAAG              
StuI          549  AGGCCT               AGG^CCT
              609  AGGCCT              
TfiI           91  GATTC                G^A[AT]TC
              154  GAATC               
TseI           70  GCTGC                G^C[AT]GC
              376  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AciI        AluI        BmyI        BsiYI       BslI        
Bsp1286I    BssKI       Cac8I       DdeI        Fnu4HI      
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeIII      MnlI        MspI        MwoI        NlaIV       
ScrFI       SfaNI       SfcI        Tsp45I      Tsp509I     
TspRI       
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