Restriction map for pF1KE0140
 (Circular) (Six-base) MAPSORT of: pF1KE0140.seq  Check: 3936  from: 1  to: 3968



pF1KE0140  3968 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:    581    1006     581 
   Size:        425    3543
 
Acc65I G'GTAC_C

Cuts at:    984     984 
   Size:       3968
 
AclI AA'CG_TT

Cuts at:   3808    3808 
   Size:       3968
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    920    1793    2225    2972    3240     920 
   Size:        873     432     747     268    1648

  Fragments arranged by size: 

                1648     873     747     432     268
 
AflII C'TTAA_G

Cuts at:    600     600 
   Size:       3968
 
AflIII A'CryG_T

Cuts at:   1320    2424    3401    1320 
   Size:       1104     977    1887

  Fragments arranged by size: 

                1887    1104     977
 
AgeI A'CCGG_T

Cuts at:    452    2356     452 
   Size:       1904    2064
 
AlwNI CAG_nnn'CTG

Cuts at:    625    1273    2840     625 
   Size:        648    1567    1753

  Fragments arranged by size: 

                1753    1567     648
 
ApaLI G'TGCA_C

Cuts at:   2738    2738 
   Size:       3968
 
ApoI r'AATT_y

Cuts at:    314     440     972    3321    3577     314 
   Size:        126     532    2349     256     705

  Fragments arranged by size: 

                2349     705     532     256     126
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3968
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3968
 
AvaI C'yCGr_G

Cuts at:    167     988     167 
   Size:        821    3147
 
BamHI G'GATC_C

Cuts at:    993     993 
   Size:       3968
 
BanI G'GyrC_C

Cuts at:    114     126     984    1398    1646    1681     114 
   Size:         12     858     414     248      35    2401

  Fragments arranged by size: 

                2401     858     414     248      35      12
 
BanII G_rGCy'C

Cuts at:    251     982    2012     251 
   Size:        731    1030    2207

  Fragments arranged by size: 

                2207    1030     731
 
BbeI G_GCGC'C

Cuts at:   1402    1650    1402 
   Size:        248    3720
 
BciVI GTATCCnnnnn_n'

Cuts at:   1866    2627    3968    1866 
   Size:        761    1341    1866

  Fragments arranged by size: 

                1866    1341     761
 
BclI T'GATC_A

Cuts at:   1488    1488 
   Size:       3968
 
BglI GCCn_nnn'nGGC

Cuts at:    665     665 
   Size:       3968
 
BglII A'GATC_T

Cuts at:   1483    3520    1483 
   Size:       2037    1931
 
BlpI GC'TnA_GC

Cuts at:   1080    1080 
   Size:       3968
 
Bme1580I G_kGCm'C

Cuts at:    706    1593    1686    2742     706 
   Size:        887      93    1056    1932

  Fragments arranged by size: 

                1932    1056     887      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1180    1261    1596    3740    1180 
   Size:         81     335    2144    1408

  Fragments arranged by size: 

                2144    1408     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2335    2335 
   Size:       3968
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    232    1144    2023    2515    2813     232 
   Size:        912     879     492     298    1387

  Fragments arranged by size: 

                1387     912     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3672    3672 
   Size:       3968
 
BsaAI yAC'GTr

Cuts at:   1321    1321 
   Size:       3968
 
BsaBI GATnn'nnATC

Cuts at:      6    1482    3519       6 
   Size:       1476    2037     455

  Fragments arranged by size: 

                2037    1476     455
 
BsaHI Gr'CG_yC

Cuts at:   1399    1647    2349    3845    1399 
   Size:        248     702    1496    1522

  Fragments arranged by size: 

                1522    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    452    1157    1678    2356    2630    2777    3188     452 
   Size:        705     521     678     274     147     411    1232

  Fragments arranged by size: 

                1232     705     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    229     229 
   Size:       3968
 
BseYI C'CCAG_C

Cuts at:    371     518     699    1393    2151    2728    3224     371 
   Size:        147     181     694     758     577     496    1115

  Fragments arranged by size: 

                1115     758     694     577     496     181     147
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    458     458 
   Size:       3968
 
BsiEI CG_ry'CG

Cuts at:    104    1510    1556    2764     104 
   Size:       1406      46    1208    1308

  Fragments arranged by size: 

                1406    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    842     982    1760    1950    2742     842 
   Size:        140     778     190     792    2068

  Fragments arranged by size: 

                2068     792     778     190     140
 
Bsp1286I G_dGCh'C

Cuts at:    251     706     842     982    1593    1686    1760    1950    2012 
   Size:        455     136     140     611      93      74     190      62

Cuts at:   2012    2742     251 
   Size:        730    1477

  Fragments arranged by size: 

                1477     730     611     455     190     140     136      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3188    3188 
   Size:       3968
 
BspHI T'CATG_A

Cuts at:   3144    3144 
   Size:       3968
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1018    1232    1534    1915    1018 
   Size:        214     302     381    3071

  Fragments arranged by size: 

                3071     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2260    2314    3798    3963      50 
   Size:       2210      54    1484     165      55

  Fragments arranged by size: 

                2210    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1880    1880 
   Size:       3968
 
BsrFI r'CCGG_y

Cuts at:    123     452    1966    2147    2356     123 
   Size:        329    1514     181     209    1735

  Fragments arranged by size: 

                1735    1514     329     209     181
 
BssSI C'ACGA_G

Cuts at:    491    2239    2597     491 
   Size:       1748     358    1862

  Fragments arranged by size: 

                1862    1748     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    481    1345     481 
   Size:        864    3104
 
BstBI TT'CG_AA

Cuts at:   2329    2329 
   Size:       3968
 
BstYI r'GATC_y

Cuts at:      1     993    1460    1483    1818    2064    3076    3162    3174 
   Size:        992     467      23     335     246    1012      86      12

Cuts at:   3174    3520       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     992     467     449     346     335     246      86
                  23      12
 
BstZ17I GTA'TAC

Cuts at:    582     582 
   Size:       3968
 
BtgI C'CryG_G

Cuts at:    107     130     107 
   Size:         23    3945
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1918    2099    2262    1918 
   Size:        181     163    3624

  Fragments arranged by size: 

                3624     181     163
 
BtsI GCAGTG_nn'

Cuts at:    542     910    1211     542 
   Size:        368     301    3299

  Fragments arranged by size: 

                3299     368     301
 
DraI TTT'AAA

Cuts at:    967     967 
   Size:       3968
 
DraIII CAC_nnn'GTG

Cuts at:    481     481 
   Size:       3968
 
DrdI GACnn_nn'nnGTC

Cuts at:   1674    2532    1674 
   Size:        858    3110
 
EaeI y'GGCC_r

Cuts at:   1553    1727    2118    2145    3490    1553 
   Size:        174     391      27    1345    2031

  Fragments arranged by size: 

                2031    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1553    1553 
   Size:       3968
 
EarI CTCTTCn'nnn_

Cuts at:    803    1991    2201     803 
   Size:       1188     210    2570

  Fragments arranged by size: 

                2570    1188     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2176    2486    2632    3778    2176 
   Size:        310     146    1146    2366

  Fragments arranged by size: 

                2366    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    980     980 
   Size:       3968
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    920    1793    2225    2972    3240     920 
   Size:        873     432     747     268    1648

  Fragments arranged by size: 

                1648     873     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    713     713 
   Size:       3968
 
EcoO109I rG'GnC_Cy

Cuts at:    324    1107     324 
   Size:        783    3185
 
EcoRI G'AATT_C

Cuts at:    972     972 
   Size:       3968
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1987    1987 
   Size:       3968
 
FspI TGC'GCA

Cuts at:   1749    1749 
   Size:       3968
 
HaeII r_GCGC'y

Cuts at:    519    1402    1650    2672    3651     519 
   Size:        883     248    1022     979     836

  Fragments arranged by size: 

                1022     979     883     836     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1810    1840    1842    1872    1810 
   Size:         30       2      30    3906

  Fragments arranged by size: 

                3906      30      30       2
 
HincII GTy'rAC

Cuts at:   1007    1007 
   Size:       3968
 
KasI G'GCGC_C

Cuts at:   1398    1646    1398 
   Size:        248    3720
 
KpnI G_GTAC'C

Cuts at:    988     988 
   Size:       3968
 
MluI A'CGCG_T

Cuts at:   3401    3401 
   Size:       3968
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    505    2639    2823     505 
   Size:       2134     184    1650

  Fragments arranged by size: 

                2134    1650     184
 
MscI TGG'CCA

Cuts at:   1729    1729 
   Size:       3968
 
MslI CAynn'nnrTG

Cuts at:    112    1957    2084     112 
   Size:       1845     127    1996

  Fragments arranged by size: 

                1996    1845     127
 
MspA1I CmG'CkG

Cuts at:    132    1079    1393    1753    2766    3011     132 
   Size:        947     314     360    1013     245    1089

  Fragments arranged by size: 

                1089    1013     947     360     314     245
 
NaeI GCC'GGC

Cuts at:   2149    2149 
   Size:       3968
 
NarI GG'CG_CC

Cuts at:   1399    1647    1399 
   Size:        248    3720
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3968
 
NgoMIV G'CCGG_C

Cuts at:   2147    2147 
   Size:       3968
 
NspI r_CATG'y

Cuts at:   1021    1521    2428    1021 
   Size:        500     907    2561

  Fragments arranged by size: 

                2561     907     500
 
PciI A'CATG_T

Cuts at:   2424    2424 
   Size:       3968
 
PfoI T'CCnGG_A

Cuts at:    281     281 
   Size:       3968
 
PmeI GTTT'AAAC

Cuts at:    967     967 
   Size:       3968
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3107    3139    3107 
   Size:         32    3936
 
PpuMI rG'GwC_Cy

Cuts at:    324     324 
   Size:       3968
 
PstI C_TGCA'G

Cuts at:    537    1015    1700     537 
   Size:        478     685    2805

  Fragments arranged by size: 

                2805     685     478
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3968
 
PvuII CAG'CTG

Cuts at:   1393    1753    1393 
   Size:        360    3608
 
RsrII CG'GwC_CG

Cuts at:   2163    2163 
   Size:       3968
 
SacI G_AGCT'C

Cuts at:    982     982 
   Size:       3968
 
SacII CC_GC'GG

Cuts at:    133     133 
   Size:       3968
 
SalI G'TCGA_C

Cuts at:   1005    1005 
   Size:       3968
 
SapI GCTCTTCn'nnn_

Cuts at:   1991    2201    1991 
   Size:        210    3758
 
SbfI CC_TGCA'GG

Cuts at:   1015    1015 
   Size:       3968
 
ScaI AGT'ACT

Cuts at:    742    3485     742 
   Size:       2743    1225
 
SfcI C'TryA_G

Cuts at:     33     144     353     533     710    1011    1696    2689    2880 
   Size:        111     209     180     177     301     685     993     191

Cuts at:   2880      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     301     209     191     180     177
                 111
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    665     665 
   Size:       3968
 
SfoI GGC'GCC

Cuts at:   1400    1648    1400 
   Size:        248    3720
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3968
 
SmaI CCC'GGG

Cuts at:    990     990 
   Size:       3968
 
SmlI C'TyrA_G

Cuts at:    211     600    1123    2038    2530    2792     211 
   Size:        389     523     915     492     262    1387

  Fragments arranged by size: 

                1387     915     523     492     389     262
 
SpeI A'CTAG_T

Cuts at:     67     568      67 
   Size:        501    3467
 
SphI G_CATG'C

Cuts at:   1021    1521    1021 
   Size:        500    3468
 
StuI AGG'CCT

Cuts at:    659     719     659 
   Size:         60    3908
 
StyI C'CwwG_G

Cuts at:    107    1102    1456     107 
   Size:        995     354    2619

  Fragments arranged by size: 

                2619     995     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    363    1551    1684    2143    2352     363 
   Size:       1188     133     459     209    1979

  Fragments arranged by size: 

                1979    1188     459     209     133
 
TatI w'GTAC_w

Cuts at:    740    3483     740 
   Size:       2743    1225
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3485    3517    3485 
   Size:         32    3936
 
Tth111I GACn'n_nGTC

Cuts at:   1765    1765 
   Size:       3968
 
XbaI T'CTAG_A

Cuts at:    999     999 
   Size:       3968
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1463    1463 
   Size:       3968
 
XmaI C'CCGG_G

Cuts at:    988     988 
   Size:       3968
 
XmnI GAAnn'nnTTC

Cuts at:    933     933 
   Size:       3968
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI   Bsu36I     ClaI    CspCI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SanDI    SexAI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com