Restriction map for pF1KB5440
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AlwNI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        Hin1I       
HindIII     HpaI        HphI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmeI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        Tsp509I     
Tth111I     XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AhdI           43  GACGGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        969  GTGCAC               G^TGCAC
AlwI          369  GATCC                GGATC(4/5)
AocI          532  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       426  AGCGCT               AGC^GCT
ApaLI         969  GTGCAC               G^TGCAC
AspEI          43  GACGGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI         550  CACGTG               CAC^GTG
BlpI          150  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      150  GCTCAGC              GC^T[ACGT]AGC
BsaI          199  GAGACC               GGTCTC(1/5)
BsgI          918  CTGCAC               GTGCAG(16/14)
BsmAI         199  GAGAC                GTCTC(1/5)
BstYI         368  AGATCC               [GA]^GATC[CT]
Bsu36I        532  CCTGAGG              CC^T[ACGT]AGG
CelII         150  GCTCAGC              GC^T[ACGT]AGC
DpnII         369  GATC                 ^GATC
Eam1105I       43  GACGGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          792  GAAGAG               CTCTTC(1/4)
Eco47III      426  AGCGCT               AGC^GCT
Eco81I        532  CCTGAGG              CC^T[ACGT]AGG
EcoNI           8  CCTCGCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         426  AGCGCT               [GA]GCGC^[CT]
HgaI          259  GACGC                GACGC(5/10)
HincII        490  GTTGAC               GT[CT]^[GA]AC
HindII        490  GTTGAC               GT[CT]^[GA]AC
HinfI         740  GAATC                G^A[ACGT]TC
Ksp632I       792  GAAGAG               CTCTTC(1/4)
MaeIII        947  GTGAC                ^GT[ACGT]AC
MboI          369  GATC                 ^GATC
MflI          368  AGATCC               [GA]^GATC[CT]
MseI          704  TTAA                 T^TAA
NdeII         369  GATC                 ^GATC
NspI          341  ACATGC               [GA]CATG^[CT]
PmaCI         550  CACGTG               CAC^GTG
PmlI          550  CACGTG               CAC^GTG
PshAI         591  GACCTATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          145  CTGCAG               CTGCA^G
Sau3AI        369  GATC                 ^GATC
SexAI         395  ACCAGGT              A^CC[AT]GGT
SfcI          145  CTGCAG               C^T[GA][CT]AG
SmaI          173  CCCGGG               CCC^GGG
StuI          855  AGGCCT               AGG^CCT
TaqI          314  TCGA                 T^CGA
TfiI          740  GAATC                G^A[AT]TC
Tru9I         704  TTAA                 T^TAA
Tsp45I        947  GTGAC                ^GT[GC]AC
TthHB8I       314  TCGA                 T^CGA
XhoII         368  AGATCC               [GA]^GATC[CT]
XmaCI         173  CCCGGG               C^CCGGG
XmaI          173  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          753  GTAC                 GT^AC
              873  GTAC                
BanI          294  GGTGCC               G^G[CT][GA]CC
              374  GGCACC              
BpmI          718  CTCCAG               CTGGAG(16/14)
              448  CTGGAG              
BsaAI         385  TACGTG               [CT]AC^GT[GA]
              550  CACGTG              
BseRI         316  GAGGAG               GAGGAG(10/8)
              511  GAGGAG              
BsrDI         474  CATTGC               GCAATG(2/0)
              641  GCAATG              
BsrFI         632  ACCGGC               [GA]^CCGG[CT]
              695  ACCGGC              
BsrI          248  ACTGG                ACTGG(1/-1)
              748  ACTGG               
Cac8I         109  GCCTGC               GC[ACGT]^[ACGT]GC
              220  GCCTGC              
Cfr10I        632  ACCGGC               [GA]^CCGG[CT]
              695  ACCGGC              
FauI          887  CCCGC                CCCGC(4/6)
              908  GCGGG               
MspA1I         28  CAGCTG               C[AC]G^C[GT]G
              153  CAGCTG              
PflMI         276  CCACCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              959  CCACACCCTGG         
PvuII          28  CAGCTG               CAG^CTG
              153  CAGCTG              
RsaI          753  GTAC                 GT^AC
              873  GTAC                
SacI          319  GAGCTC               GAGCT^C
              565  GAGCTC              
Van91I        276  CCACCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              959  CCACACCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AspHI       
AvaI        AvaII       BanII       BbsI        BbvI        
BcnI        BmyI        BpuAI       BsaJI       BsiHKAI     
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       BstUI       
CfoI        Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MaeII       
MboII       MnlI        MslI        MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
Sau96I      ScrFI       SfaNI       TseI        TspRI       
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