Restriction map for pF1KB5440
 (Circular) (Six-base) MAPSORT of: pF1KB5440.seq  Check: 2138  from: 1  to: 4094



pF1KB5440  4094 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    887     887 
   Size:       4094
 
AccI GT'mk_AC

Cuts at:   1132    1132 
   Size:       4094
 
Acc65I G'GTAC_C

Cuts at:   1110    1110 
   Size:       4094
 
AclI AA'CG_TT

Cuts at:   3934    3934 
   Size:       4094
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    501    1919    2351    3098    3366     501 
   Size:       1418     432     747     268    1229

  Fragments arranged by size: 

                1418    1229     747     432     268
 
AfeI AGC'GCT

Cuts at:    536     536 
   Size:       4094
 
AflIII A'CryG_T

Cuts at:   1446    2550    3527    1446 
   Size:       1104     977    2013

  Fragments arranged by size: 

                2013    1104     977
 
AgeI A'CCGG_T

Cuts at:   2482    2482 
   Size:       4094
 
AhdI GACnn_n'nnGTC

Cuts at:    156     156 
   Size:       4094
 
AleI CACnn'nnGTG

Cuts at:   1074    1074 
   Size:       4094
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    736     736 
   Size:       4094
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    781     813     781 
   Size:         32    4062
 
AlwNI CAG_nnn'CTG

Cuts at:   1399    2966    1399 
   Size:       1567    2527
 
ApaI G_GGCC'C

Cuts at:    319     382     653    1023     319 
   Size:         63     271     370    3390

  Fragments arranged by size: 

                3390     370     271      63
 
ApaLI G'TGCA_C

Cuts at:   1077    2864    1077 
   Size:       1787    2307
 
ApoI r'AATT_y

Cuts at:   1098    3447    3703    1098 
   Size:       2349     256    1489

  Fragments arranged by size: 

                2349    1489     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4094
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4094
 
AvaI C'yCGr_G

Cuts at:    281     312     843    1114     281 
   Size:         31     531     271    3261

  Fragments arranged by size: 

                3261     531     271      31
 
BamHI G'GATC_C

Cuts at:   1119    1119 
   Size:       4094
 
BanI G'GyrC_C

Cuts at:    402     482    1110    1524    1772    1807     402 
   Size:         80     628     414     248      35    2689

  Fragments arranged by size: 

                2689     628     414     248      80      35
 
BanII G_rGCy'C

Cuts at:    319     382     431     653     677     827    1023    1108    2138 
   Size:         63      49     222      24     150     196      85    1030

Cuts at:   2138     319 
   Size:       2275

  Fragments arranged by size: 

                2275    1030     222     196     150      85      63      49
                  24
 
BbeI G_GCGC'C

Cuts at:   1528    1776    1528 
   Size:        248    3846
 
BbsI GAAGACnn'nnnn_

Cuts at:    192     371     429     192 
   Size:        179      58    3857

  Fragments arranged by size: 

                3857     179      58
 
BciVI GTATCCnnnnn_n'

Cuts at:   1992    2753    4094    1992 
   Size:        761    1341    1992

  Fragments arranged by size: 

                1992    1341     761
 
BclI T'GATC_A

Cuts at:   1614    1614 
   Size:       4094
 
BglII A'GATC_T

Cuts at:   1609    3646    1609 
   Size:       2037    2057
 
BlpI GC'TnA_GC

Cuts at:    259    1206     259 
   Size:        947    3147
 
Bme1580I G_kGCm'C

Cuts at:    319     382     407     446     653    1023    1081    1719    1812 
   Size:         63      25      39     207     370      58     638      93

Cuts at:   1812    2868     319 
   Size:       1056    1545

  Fragments arranged by size: 

                1545    1056     638     370     207      93      63      58
                  39      25
 
BmgBI CAC'GTC

Cuts at:    276     276 
   Size:       4094
 
BmrI ACTGGGnnnn_n'

Cuts at:    366     866    1306    1387    1722    3866     366 
   Size:        500     440      81     335    2144     594

  Fragments arranged by size: 

                2144     594     500     440     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    841    2461     841 
   Size:       1620    2474
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    577     811     577 
   Size:        234    3860
 
Bpu10I CC'TnA_GC

Cuts at:    350     463     350 
   Size:        113    3981
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1270    2149    2641    2939    1270 
   Size:        879     492     298    2425

  Fragments arranged by size: 

                2425     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    301    3798     301 
   Size:       3497     597
 
BsaAI yAC'GTr

Cuts at:    495     660    1447     495 
   Size:        165     787    3142

  Fragments arranged by size: 

                3142     787     165
 
BsaBI GATnn'nnATC

Cuts at:      6    1608    3645       6 
   Size:       1602    2037     455

  Fragments arranged by size: 

                2037    1602     455
 
BsaHI Gr'CG_yC

Cuts at:   1525    1773    2475    3971    1525 
   Size:        248     702    1496    1648

  Fragments arranged by size: 

                1648    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1283    1804    2482    2756    2903    3314    1283 
   Size:        521     678     274     147     411    2063

  Fragments arranged by size: 

                2063     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    649     679     649 
   Size:         30    4064
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    439     634     439 
   Size:        195    3899
 
BseYI C'CCAG_C

Cuts at:    228     646     776     820     888    1519    2277    2854    3350 
   Size:        418     130      44      68     631     758     577     496

Cuts at:   3350     228 
   Size:        972

  Fragments arranged by size: 

                 972     758     631     577     496     418     130      68
                  44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1011    1011 
   Size:       4094
 
BsiEI CG_ry'CG

Cuts at:    104    1636    1682    2890     104 
   Size:       1532      46    1208    1308

  Fragments arranged by size: 

                1532    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    356     431     500     677    1081    1108    1886    2076    2868 
   Size:         75      69     177     404      27     778     190     792

Cuts at:   2868     356 
   Size:       1582

  Fragments arranged by size: 

                1582     792     778     404     190     177      75      69
                  27
 
Bsp1286I G_dGCh'C

Cuts at:    319     356     382     407     431     446     500     653     677 
   Size:         37      26      25      24      15      54     153      24

Cuts at:    677     827    1023    1081    1108    1719    1812    1886    2076 
   Size:        150     196      58      27     611      93      74     190

Cuts at:   2076    2138    2868     319 
   Size:         62     730    1545

  Fragments arranged by size: 

                1545     730     611     196     190     153     150      93
                  74      62      58      54      37      27      26      25
                  24      24      15
 
BspEI T'CCGG_A

Cuts at:   3314    3314 
   Size:       4094
 
BspHI T'CATG_A

Cuts at:   3270    3270 
   Size:       4094
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    146     611     887    1144    1358    1660    2041     146 
   Size:        465     276     257     214     302     381    2199

  Fragments arranged by size: 

                2199     465     381     302     276     257     214
 
BsrBI CCG'CTC

Cuts at:     50    2386    2440    3924    4089      50 
   Size:       2336      54    1484     165      55

  Fragments arranged by size: 

                2336    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    581     756    2006     581 
   Size:        175    1250    2669

  Fragments arranged by size: 

                2669    1250     175
 
BsrFI r'CCGG_y

Cuts at:    740     803    2092    2273    2482     740 
   Size:         63    1289     181     209    2352

  Fragments arranged by size: 

                2352    1289     209     181      63
 
BssSI C'ACGA_G

Cuts at:   2365    2723    2365 
   Size:        358    3736
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1471    1471 
   Size:       4094
 
BstBI TT'CG_AA

Cuts at:   2455    2455 
   Size:       4094
 
BstYI r'GATC_y

Cuts at:      1     476    1119    1586    1609    1944    2190    3202    3288 
   Size:        475     643     467      23     335     246    1012      86

Cuts at:   3288    3300    3646       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     643     475     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    641     641 
   Size:       4094
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    903    2044    2225    2388     903 
   Size:       1141     181     163    2609

  Fragments arranged by size: 

                2609    1141     181     163
 
BtsI GCAGTG_nn'

Cuts at:    401     998    1337     401 
   Size:        597     339    3158

  Fragments arranged by size: 

                3158     597     339
 
DraI TTT'AAA

Cuts at:   1093    1093 
   Size:       4094
 
DrdI GACnn_nn'nnGTC

Cuts at:   1800    2658    1800 
   Size:        858    3236
 
EaeI y'GGCC_r

Cuts at:   1679    1853    2244    2271    3616    1679 
   Size:        174     391      27    1345    2157

  Fragments arranged by size: 

                2157    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1679    1679 
   Size:       4094
 
EarI CTCTTCn'nnn_

Cuts at:    895    2117    2327     895 
   Size:       1222     210    2662

  Fragments arranged by size: 

                2662    1222     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    826     912    2302    2612    2758    3904     826 
   Size:         86    1390     310     146    1146    1016

  Fragments arranged by size: 

                1390    1146    1016     310     146      86
 
EcoICRI GAG'CTC

Cuts at:    429     675    1106     429 
   Size:        246     431    3417

  Fragments arranged by size: 

                3417     431     246
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    501     577     811    1919    2351    3098    3366     501 
   Size:         76     234    1108     432     747     268    1229

  Fragments arranged by size: 

                1229    1108     747     432     268     234      76
 
EcoNI CCTnn'n_nnAGG

Cuts at:    120     120 
   Size:       4094
 
EcoO109I rG'GnC_Cy

Cuts at:    316     378     379     650    1019    1020    1233     316 
   Size:         62       1     271     369       1     213    3177

  Fragments arranged by size: 

                3177     369     271     213      62       1       1
 
EcoRI G'AATT_C

Cuts at:   1098    1098 
   Size:       4094
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2113    2113 
   Size:       4094
 
FspI TGC'GCA

Cuts at:   1875    1875 
   Size:       4094
 
HaeII r_GCGC'y

Cuts at:    538    1528    1776    2798    3777     538 
   Size:        990     248    1022     979     855

  Fragments arranged by size: 

                1022     990     979     855     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    142     174    1936    1966    1968    1998     142 
   Size:         32    1762      30       2      30    2238

  Fragments arranged by size: 

                2238    1762      32      30      30       2
 
HincII GTy'rAC

Cuts at:    600    1133     600 
   Size:        533    3561
 
KasI G'GCGC_C

Cuts at:   1524    1772    1524 
   Size:        248    3846
 
KpnI G_GTAC'C

Cuts at:   1114    1114 
   Size:       4094
 
MluI A'CGCG_T

Cuts at:   3527    3527 
   Size:       4094
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    785    1028    2765    2949     785 
   Size:        243    1737     184    1930

  Fragments arranged by size: 

                1930    1737     243     184
 
MscI TGG'CCA

Cuts at:   1855    1855 
   Size:       4094
 
MslI CAynn'nnrTG

Cuts at:    493     544     586     881    1074    2083    2210     493 
   Size:         51      42     295     193    1009     127    2377

  Fragments arranged by size: 

                2377    1009     295     193     127      51      42
 
MspA1I CmG'CkG

Cuts at:    138     263    1205    1519    1879    2892    3137     138 
   Size:        125     942     314     360    1013     245    1095

  Fragments arranged by size: 

                1095    1013     942     360     314     245     125
 
NaeI GCC'GGC

Cuts at:   2275    2275 
   Size:       4094
 
NarI GG'CG_CC

Cuts at:   1525    1773    1525 
   Size:        248    3846
 
NgoMIV G'CCGG_C

Cuts at:   2273    2273 
   Size:       4094
 
NspI r_CATG'y

Cuts at:    453    1147    1647    2554     453 
   Size:        694     500     907    1993

  Fragments arranged by size: 

                1993     907     694     500
 
PasI CC'CwG_GG

Cuts at:    143     143 
   Size:       4094
 
PciI A'CATG_T

Cuts at:   2550    2550 
   Size:       4094
 
PflMI CCAn_nnn'nTGG

Cuts at:    390    1073     390 
   Size:        683    3411
 
PfoI T'CCnGG_A

Cuts at:    554     554 
   Size:       4094
 
PmeI GTTT'AAAC

Cuts at:   1093    1093 
   Size:       4094
 
PmlI CAC'GTG

Cuts at:    660     660 
   Size:       4094
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3233    3265    3233 
   Size:         32    4062
 
PshAI GACnn'nnGTC

Cuts at:    703     703 
   Size:       4094
 
PspOMI G'GGCC_C

Cuts at:    315     378     649    1019     315 
   Size:         63     271     370    3390

  Fragments arranged by size: 

                3390     370     271      63
 
PstI C_TGCA'G

Cuts at:    257    1141    1826     257 
   Size:        884     685    2525

  Fragments arranged by size: 

                2525     884     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4094
 
PvuII CAG'CTG

Cuts at:    138     263    1519    1879     138 
   Size:        125    1256     360    2353

  Fragments arranged by size: 

                2353    1256     360     125
 
RsrII CG'GwC_CG

Cuts at:   2289    2289 
   Size:       4094
 
SacI G_AGCT'C

Cuts at:    431     677    1108     431 
   Size:        246     431    3417

  Fragments arranged by size: 

                3417     431     246
 
SalI G'TCGA_C

Cuts at:   1131    1131 
   Size:       4094
 
SapI GCTCTTCn'nnn_

Cuts at:   2117    2327    2117 
   Size:        210    3884
 
SbfI CC_TGCA'GG

Cuts at:   1141    1141 
   Size:       4094
 
ScaI AGT'ACT

Cuts at:   3611    3611 
   Size:       4094
 
SexAI A'CCwGG_T

Cuts at:    503     503 
   Size:       4094
 
SfcI C'TryA_G

Cuts at:     33     253    1137    1822    2815    3006      33 
   Size:        220     884     685     993     191    1121

  Fragments arranged by size: 

                1121     993     884     685     220     191
 
SfoI GGC'GCC

Cuts at:   1526    1774    1526 
   Size:        248    3846
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4094
 
SmaI CCC'GGG

Cuts at:    283    1116     283 
   Size:        833    3261
 
SmlI C'TyrA_G

Cuts at:   1249    2164    2656    2918    1249 
   Size:        915     492     262    2425

  Fragments arranged by size: 

                2425     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4094
 
SphI G_CATG'C

Cuts at:   1147    1647    1147 
   Size:        500    3594
 
SrfI GCCC'GGGC

Cuts at:    283     283 
   Size:       4094
 
StuI AGG'CCT

Cuts at:    965     965 
   Size:       4094
 
StyI C'CwwG_G

Cuts at:   1228    1582    1228 
   Size:        354    3740
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1677    1810    2269    2478    1677 
   Size:        133     459     209    3293

  Fragments arranged by size: 

                3293     459     209     133
 
TatI w'GTAC_w

Cuts at:   3609    3609 
   Size:       4094
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    649     681    3611    3643     649 
   Size:         32    2930      32    1100

  Fragments arranged by size: 

                2930    1100      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1891    1891 
   Size:       4094
 
XbaI T'CTAG_A

Cuts at:   1125    1125 
   Size:       4094
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1589    1589 
   Size:       4094
 
XmaI C'CCGG_G

Cuts at:    281    1114     281 
   Size:        833    3261
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI   Bsu36I    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII    AflII     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI
     BglI     BmtI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I     BtgI     ClaI    CspCI   DraIII    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI    PpuMI     PsiI    PspXI     PsrI    SacII
    SanDI     SfiI    SgrAI    SnaBI     SspI     SwaI     XhoI     XmnI
     ZraI
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