Restriction map for pF1KB6125
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaII       AvrII       BalI        BamHI       BbrPI       
BbsI        BcnI        BfrI        BglII       BlnI        
BpuAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmFI       BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       Bst1107I    BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraII       
Eam1105I    Eco47III    Eco81I      EcoO109I    EcoO65I     
EcoRV       EcoT14I     EcoT22I     FseI        HpaI        
KpnI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        NaeI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SgrAI       SmaI        
SpeI        Sse8387I    SspI        StuI        StyI        
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AlwNI        1310  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI         1202  CCCGAG               C^[CT]CG[GA]G
AviII         149  TGCGCA               TGC^GCA
BanI          388  GGCGCC               G^G[CT][GA]CC
BbeI          388  GGCGCC               GGCGC^C
BbvCI        1307  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1198  GTATCC               GTATCC(6/5)
BclI         1412  TGATCA               T^GATCA
BglI           38  GCCGCGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           63  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       63  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1226  TACGTA               [CT]AC^GT[GA]
BsgI          452  CTGCAC               GTGCAG(16/14)
BsiEI         213  CGGCCG               CG[GA][CT]^CG
BsmBI         160  GAGACG               CGTCTC(1/5)
BsmI          819  GAATGC               GAATGC(1/-1)
BsoBI        1202  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      134  TGTACA               T^GTACA
BspHI         738  TCATGA               T^CATGA
BspMI         186  GCAGGT               ACCTGC(4/8)
BsrGI         134  TGTACA               T^GTACA
BssSI        1557  CTCGTG               CACGAG(-5/-1)
BstAPI        439  GCAGCTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         429  TTCGAA               TT^CGAA
BstYI         492  GGATCT               [GA]^GATC[CT]
CelII          63  GCTGAGC              GC^T[ACGT]AGC
DraI         1636  TTTAAA               TTT^AAA
DraIII        455  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1429  GACACATCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           25  CCGCGG               C^C[GA][CT]GG
EagI          213  CGGCCG               C^GGCCG
EclXI         213  CGGCCG               C^GGCCG
Eco52I        213  CGGCCG               C^GGCCG
EcoNI         600  CCTTACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         426  GAATTC               G^AATTC
FauI          290  CCCGC                CCCGC(4/6)
FbaI         1412  TGATCA               T^GATCA
FspI          149  TGCGCA               TGC^GCA
HincII        856  GTCAAC               GT[CT]^[GA]AC
HindII        856  GTCAAC               GT[CT]^[GA]AC
HindIII       400  AAGCTT               A^AGCTT
KasI          388  GGCGCC               G^GCGCC
KspI           25  CCGCGG               CCGC^GG
MaeII        1227  ACGT                 A^CGT
MflI          492  GGATCT               [GA]^GATC[CT]
NarI          388  GGCGCC               GG^CGCC
NspV          429  TTCGAA               TT^CGAA
PleI          937  GAGTC                GAGTC(4/5)
RcaI          738  TCATGA               T^CATGA
SacII          25  CCGCGG               CCGC^GG
SfoI          388  GGCGCC               GGC^GCC
SfuI          429  TTCGAA               TT^CGAA
SnaBI        1226  TACGTA               TAC^GTA
SphI         1010  GCATGC               GCATG^C
SspBI         134  TGTACA               T^GTACA
XbaI          940  TCTAGA               T^CTAGA
XcmI         1393  CCAATGGGAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         492  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          388  GGCGCC               G[GA]^CG[CT]C
              698  GGCGTC              
Asp700I       955  GAAAGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1081  GAACAGCTTC          
AspHI         112  GAGCTC               G[AT]GC[AT]^C
              220  GAGCTC              
BcgI           11  CGACCCCCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              421  GCACTGAATTCG        
BfaI          941  CTAG                 C^TAG
             1235  CTAG                
BsaHI         388  GGCGCC               G[GA]^CG[CT]C
              698  GGCGTC              
BsaI          247  GAGACC               GGTCTC(1/5)
              652  GAGACC              
BsiHKAI       112  GAGCTC               G[AT]GC[AT]^C
              220  GAGCTC              
BsrFI         122  ACCGGC               [GA]^CCGG[CT]
              226  ACCGGC              
Cfr10I        122  ACCGGC               [GA]^CCGG[CT]
              226  ACCGGC              
Cfr13I         49  GGCCC                G^G[ACGT]CC
              288  GGCCC               
EaeI          213  CGGCCG               [CT]^GGCC[GA]
              257  TGGCCG              
HaeII         237  GGCGCT               [GA]GCGC^[CT]
              388  GGCGCC              
HapII         123  CCGG                 C^CGG
              227  CCGG                
Hin1I         388  GGCGCC               G[GA]^CG[CT]C
              698  GGCGTC              
HpaII         123  CCGG                 C^CGG
              227  CCGG                
MaeI          941  CTAG                 C^TAG
             1235  CTAG                
MspI          123  CCGG                 C^CGG
              227  CCGG                
SacI          112  GAGCTC               GAGCT^C
              220  GAGCTC              
Sau96I         49  GGCCC                G^G[ACGT]CC
              288  GGCCC               
XmnI          955  GAAAGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1081  GAACAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        BanII       BbvI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HgaI        HhaI        HinP1I      
HinfI       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PstI        PvuII       RsaI        
Sau3AI      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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