Restriction map for pF1KB6125
 (Circular) (Six-base) MAPSORT of: pF1KB6125.seq  Check: 7684  from: 1  to: 4871



pF1KB6125  4871 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    285     285 
   Size:       4871
 
AccI GT'mk_AC

Cuts at:   1909    1909 
   Size:       4871
 
Acc65I G'GTAC_C

Cuts at:   1887    1887 
   Size:       4871
 
AclI AA'CG_TT

Cuts at:   4711    4711 
   Size:       4871
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    625    1597    1781    2696    3128    3875    4143     625 
   Size:        972     184     915     432     747     268    1353

  Fragments arranged by size: 

                1353     972     915     747     432     268     184
 
AflIII A'CryG_T

Cuts at:   2223    3327    4304    2223 
   Size:       1104     977    2790

  Fragments arranged by size: 

                2790    1104     977
 
AgeI A'CCGG_T

Cuts at:   3259    3259 
   Size:       4871
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    884     884 
   Size:       4871
 
AlwNI CAG_nnn'CTG

Cuts at:   1423    2176    3743    1423 
   Size:        753    1567    2551

  Fragments arranged by size: 

                2551    1567     753
 
ApaLI G'TGCA_C

Cuts at:   3641    3641 
   Size:       4871
 
ApoI r'AATT_y

Cuts at:    534    1462    1704    1875    4224    4480     534 
   Size:        928     242     171    2349     256     925

  Fragments arranged by size: 

                2349     928     925     256     242     171
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4871
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4871
 
AvaI C'yCGr_G

Cuts at:   1310    1891    1310 
   Size:        581    4290
 
BamHI G'GATC_C

Cuts at:   1896    1896 
   Size:       4871
 
BanI G'GyrC_C

Cuts at:    496    1887    2301    2549    2584     496 
   Size:       1391     414     248      35    2783

  Fragments arranged by size: 

                2783    1391     414     248      35
 
BanII G_rGCy'C

Cuts at:    178     224     332    1885    2915     178 
   Size:         46     108    1553    1030    2134

  Fragments arranged by size: 

                2134    1553    1030     108      46
 
BbeI G_GCGC'C

Cuts at:    500    2305    2553     500 
   Size:       1805     248    2818

  Fragments arranged by size: 

                2818    1805     248
 
BbvCI CC'TCA_GC

Cuts at:   1416    1416 
   Size:       4871
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    108     142     518     552     108 
   Size:         34     376      34    4427

  Fragments arranged by size: 

                4427     376      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1317    2769    3530    4871    1317 
   Size:       1452     761    1341    1317

  Fragments arranged by size: 

                1452    1341    1317     761
 
BclI T'GATC_A

Cuts at:   1520    2391    1520 
   Size:        871    4000
 
BglI GCCn_nnn'nGGC

Cuts at:    152     152 
   Size:       4871
 
BglII A'GATC_T

Cuts at:   2386    4423    2386 
   Size:       2037    2834
 
BlpI GC'TnA_GC

Cuts at:    172    1983     172 
   Size:       1811    3060
 
Bme1580I G_kGCm'C

Cuts at:   1675    2496    2589    3645    1675 
   Size:        821      93    1056    2901

  Fragments arranged by size: 

                2901    1056     821      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    615    2083    2164    2499    4643     615 
   Size:       1468      81     335    2144     843

  Fragments arranged by size: 

                2144    1468     843     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3238    3238 
   Size:       4871
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    181     286     919    1811     181 
   Size:        105     633     892    3241

  Fragments arranged by size: 

                3241     892     633     105
 
Bpu10I CC'TnA_GC

Cuts at:    636    1416     636 
   Size:        780    4091
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    325     943    1305    1548    2047    2926    3418    3716     325 
   Size:        618     362     243     499     879     492     298    1480

  Fragments arranged by size: 

                1480     879     618     499     492     362     298     243
            
 
BsaI GGTCTCn'nnnn_

Cuts at:    349     754    4575     349 
   Size:        405    3821     645

  Fragments arranged by size: 

                3821     645     405
 
BsaAI yAC'GTr

Cuts at:   1336    2224    1336 
   Size:        888    3983
 
BsaBI GATnn'nnATC

Cuts at:      6    2385    4422       6 
   Size:       2379    2037     455

  Fragments arranged by size: 

                2379    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    497     807    2302    2550    3252    4748     497 
   Size:        310    1495     248     702    1496     620

  Fragments arranged by size: 

                1496    1495     702     620     310     248
 
BsaWI w'CCGG_w

Cuts at:   2060    2581    3259    3533    3680    4091    2060 
   Size:        521     678     274     147     411    2840

  Fragments arranged by size: 

                2840     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1706    1736    1784    1814    1706 
   Size:         30      48      30    4763

  Fragments arranged by size: 

                4763      48      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    223     325     451     763     907    1732    1783    1786    1789 
   Size:        102     126     312     144     825      51       3       3

Cuts at:   1789     223 
   Size:       3305

  Fragments arranged by size: 

                3305     825     312     144     126     102      51       3
                   3
 
BseYI C'CCAG_C

Cuts at:    426    1022    1511    2296    3054    3631    4127     426 
   Size:        596     489     785     758     577     496    1170

  Fragments arranged by size: 

                1170     785     758     596     577     496     489
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    545     545 
   Size:       4871
 
BsiEI CG_ry'CG

Cuts at:    104     324    2413    2459    3667     104 
   Size:        220    2089      46    1208    1308

  Fragments arranged by size: 

                2089    1308    1208     220      46
 
BsiHKAI G_wGCw'C

Cuts at:    224     332    1885    2663    2853    3645     224 
   Size:        108    1553     778     190     792    1450

  Fragments arranged by size: 

                1553    1450     792     778     190     108
 
BsmI GAATG_Cn'

Cuts at:    933     933 
   Size:       4871
 
BsmBI CGTCTCn'nnnn_

Cuts at:    262     262 
   Size:       4871
 
Bsp1286I G_dGCh'C

Cuts at:    178     224     332    1675    1885    2496    2589    2663    2853 
   Size:         46     108    1343     210     611      93      74     190

Cuts at:   2853    2915    3645     178 
   Size:         62     730    1404

  Fragments arranged by size: 

                1404    1343     730     611     210     190     108      93
                  74      62      46
 
BspEI T'CCGG_A

Cuts at:   4091    4091 
   Size:       4871
 
BspHI T'CATG_A

Cuts at:    846    4047     846 
   Size:       3201    1670
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    285    1921    2135    2437    2818     285 
   Size:       1636     214     302     381    2338

  Fragments arranged by size: 

                2338    1636     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3163    3217    4701    4866      50 
   Size:       3113      54    1484     165      55

  Fragments arranged by size: 

                3113    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2783    2783 
   Size:       4871
 
BsrFI r'CCGG_y

Cuts at:    230     334    2869    3050    3259     230 
   Size:        104    2535     181     209    1842

  Fragments arranged by size: 

                2535    1842     209     181     104
 
BsrGI T'GTAC_A

Cuts at:    242     242 
   Size:       4871
 
BssHII G'CGCG_C

Cuts at:    281     380     405     281 
   Size:         99      25    4747

  Fragments arranged by size: 

                4747      99      25
 
BssSI C'ACGA_G

Cuts at:   1665    3142    3500    1665 
   Size:       1477     358    3036

  Fragments arranged by size: 

                3036    1477     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    553    2248     553 
   Size:       1695    3176
 
BstBI TT'CG_AA

Cuts at:    538    3232     538 
   Size:       2694    2177
 
BstYI r'GATC_y

Cuts at:      1     600    1896    2363    2386    2721    2967    3979    4065 
   Size:        599    1296     467      23     335     246    1012      86

Cuts at:   4065    4077    4423       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1296    1012     599     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     133     107 
   Size:         26    4845
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2821    3002    3165    2821 
   Size:        181     163    4527

  Fragments arranged by size: 

                4527     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2114    2114 
   Size:       4871
 
DraI TTT'AAA

Cuts at:   1746    1870    1746 
   Size:        124    4747
 
DraIII CAC_nnn'GTG

Cuts at:    568     568 
   Size:       4871
 
DrdI GACnn_nn'nnGTC

Cuts at:   1543    2577    3435    1543 
   Size:       1034     858    2979

  Fragments arranged by size: 

                2979    1034     858
 
EaeI y'GGCC_r

Cuts at:    321     365    2456    2630    3021    3048    4393     321 
   Size:         44    2091     174     391      27    1345     799

  Fragments arranged by size: 

                2091    1345     799     391     174      44      27
 
EagI C'GGCC_G

Cuts at:    321    2456     321 
   Size:       2135    2736
 
EarI CTCTTCn'nnn_

Cuts at:    740    1256    1351    1760    2894    3104     740 
   Size:        516      95     409    1134     210    2507

  Fragments arranged by size: 

                2507    1134     516     409     210      95
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    457    1162    1401    3079    3389    3535    4681     457 
   Size:        705     239    1678     310     146    1146     647

  Fragments arranged by size: 

                1678    1146     705     647     310     239     146
 
EcoICRI GAG'CTC

Cuts at:    222     330    1883     222 
   Size:        108    1553    3210

  Fragments arranged by size: 

                3210    1553     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    181     286     625     919    1597    1781    1811    2696    3128 
   Size:        105     339     294     678     184      30     885     432

Cuts at:   3128    3875    4143     181 
   Size:        747     268     909

  Fragments arranged by size: 

                 909     885     747     678     432     339     294     268
                 184     105      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:    712     712 
   Size:       4871
 
EcoO109I rG'GnC_Cy

Cuts at:   2010    2010 
   Size:       4871
 
EcoRI G'AATT_C

Cuts at:    534    1875     534 
   Size:       1341    3530
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1307    2890    1307 
   Size:       1583    3288
 
FspI TGC'GCA

Cuts at:    259    2652     259 
   Size:       2393    2478
 
HaeII r_GCGC'y

Cuts at:    349     500    2305    2553    3575    4554     349 
   Size:        151    1805     248    1022     979     666

  Fragments arranged by size: 

                1805    1022     979     666     248     151
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    368     400    2713    2743    2745    2775     368 
   Size:         32    2313      30       2      30    2464

  Fragments arranged by size: 

                2464    2313      32      30      30       2
 
HincII GTy'rAC

Cuts at:    966    1910     966 
   Size:        944    3927
 
HindIII A'AGCT_T

Cuts at:    508     508 
   Size:       4871
 
KasI G'GCGC_C

Cuts at:    496    2301    2549     496 
   Size:       1805     248    2818

  Fragments arranged by size: 

                2818    1805     248
 
KpnI G_GTAC'C

Cuts at:   1891    1891 
   Size:       4871
 
MluI A'CGCG_T

Cuts at:   4304    4304 
   Size:       4871
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    605    3542    3726     605 
   Size:       2937     184    1750

  Fragments arranged by size: 

                2937    1750     184
 
MscI TGG'CCA

Cuts at:   2632    2632 
   Size:       4871
 
MslI CAynn'nnrTG

Cuts at:   2860    2987    2860 
   Size:        127    4744
 
MspA1I CmG'CkG

Cuts at:    135     171     291     456    1176    1789    1982    2296    2656 
   Size:         36     120     165     720     613     193     314     360

Cuts at:   2656    3669    3914     135 
   Size:       1013     245    1092

  Fragments arranged by size: 

                1092    1013     720     613     360     314     245     193
                 165     120      36
 
NaeI GCC'GGC

Cuts at:   3052    3052 
   Size:       4871
 
NarI GG'CG_CC

Cuts at:    497    2302    2550     497 
   Size:       1805     248    2818

  Fragments arranged by size: 

                2818    1805     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4871
 
NgoMIV G'CCGG_C

Cuts at:   3050    3050 
   Size:       4871
 
NspI r_CATG'y

Cuts at:    741    1058    1122    1924    2424    3331     741 
   Size:        317      64     802     500     907    2281

  Fragments arranged by size: 

                2281     907     802     500     317      64
 
PciI A'CATG_T

Cuts at:   3327    3327 
   Size:       4871
 
PfoI T'CCnGG_A

Cuts at:    197     197 
   Size:       4871
 
PmeI GTTT'AAAC

Cuts at:   1870    1870 
   Size:       4871
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1648    1680    4010    4042    1648 
   Size:         32    2330      32    2477

  Fragments arranged by size: 

                2477    2330      32      32
 
PsiI TTA'TAA

Cuts at:   1553    1553 
   Size:       4871
 
PstI C_TGCA'G

Cuts at:    290     296     419     645    1918    2603     290 
   Size:          6     123     226    1273     685    2558

  Fragments arranged by size: 

                2558    1273     685     226     123       6
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4871
 
PvuII CAG'CTG

Cuts at:    291     456    1176    1789    2296    2656     291 
   Size:        165     720     613     507     360    2506

  Fragments arranged by size: 

                2506     720     613     507     360     165
 
RsrII CG'GwC_CG

Cuts at:   3066    3066 
   Size:       4871
 
SacI G_AGCT'C

Cuts at:    224     332    1885     224 
   Size:        108    1553    3210

  Fragments arranged by size: 

                3210    1553     108
 
SacII CC_GC'GG

Cuts at:    136     136 
   Size:       4871
 
SalI G'TCGA_C

Cuts at:   1908    1908 
   Size:       4871
 
SapI GCTCTTCn'nnn_

Cuts at:   2894    3104    2894 
   Size:        210    4661
 
SbfI CC_TGCA'GG

Cuts at:   1918    1918 
   Size:       4871
 
ScaI AGT'ACT

Cuts at:   4388    4388 
   Size:       4871
 
SfcI C'TryA_G

Cuts at:     33     286     292     415     641    1036    1914    2599    3592 
   Size:        253       6     123     226     395     878     685     993

Cuts at:   3592    3783      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     878     685     395     253     226     191
                 123       6
 
SfoI GGC'GCC

Cuts at:    498    2303    2551     498 
   Size:       1805     248    2818

  Fragments arranged by size: 

                2818    1805     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4871
 
SmaI CCC'GGG

Cuts at:   1893    1893 
   Size:       4871
 
SmlI C'TyrA_G

Cuts at:    340     922    1320    1563    2026    2941    3433    3695     340 
   Size:        582     398     243     463     915     492     262    1516

  Fragments arranged by size: 

                1516     915     582     492     463     398     262     243
            
 
SnaBI TAC'GTA

Cuts at:   1336    1336 
   Size:       4871
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4871
 
SphI G_CATG'C

Cuts at:   1122    1924    2424    1122 
   Size:        802     500    3569

  Fragments arranged by size: 

                3569     802     500
 
StyI C'CwwG_G

Cuts at:    107    2005    2359     107 
   Size:       1898     354    2619

  Fragments arranged by size: 

                2619    1898     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    373    2454    2587    3046    3255     373 
   Size:       2081     133     459     209    1989

  Fragments arranged by size: 

                2081    1989     459     209     133
 
TatI w'GTAC_w

Cuts at:    242     824    1735    4386     242 
   Size:        582     911    2651     727

  Fragments arranged by size: 

                2651     911     727     582
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4388    4420    4388 
   Size:         32    4839
 
Tth111I GACn'n_nGTC

Cuts at:   2668    2668 
   Size:       4871
 
XbaI T'CTAG_A

Cuts at:   1048    1902    1048 
   Size:        854    4017
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1508    2366    1508 
   Size:        858    4013
 
XmaI C'CCGG_G

Cuts at:   1891    1891 
   Size:       4871
 
XmnI GAAnn'nnTTC

Cuts at:   1067    1193    1067 
   Size:        126    4745
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI     PsiI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BfrBI    BmgBI     BmtI    BsiWI   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI    FspAI
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI
    SanDI    SexAI     SfiI    SgrAI     SrfI     SspI     StuI     SwaI
     XhoI     ZraI
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