Restriction map for
pF1KB6125
(Linear) (Six-base) MAPSORT of: KIBB6125.seq Check: 432 from: 1 to: 1758
KIBB6125.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 177 1758
Size: 177 1581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1489 1673 1758
Size: 517 972 184 85
Fragments arranged by size:
972 517 184 85
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 776 1758
Size: 776 982
AlwNI CAG_nnn'CTG
Cuts at: 0 1315 1758
Size: 1315 443
ApoI r'AATT_y
Cuts at: 0 426 1354 1596 1758
Size: 426 928 242 162
Fragments arranged by size:
928 426 242 162
AvaI C'yCGr_G
Cuts at: 0 1202 1758
Size: 1202 556
BanI G'GyrC_C
Cuts at: 0 388 1758
Size: 388 1370
BanII G_rGCy'C
Cuts at: 0 70 116 224 1758
Size: 70 46 108 1534
Fragments arranged by size:
1534 108 70 46
BbeI G_GCGC'C
Cuts at: 0 392 1758
Size: 392 1366
BbvCI CC'TCA_GC
Cuts at: 0 1308 1758
Size: 1308 450
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 410 444 1758
Size: 34 376 34 1314
Fragments arranged by size:
1314 376 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1209 1758
Size: 1209 549
BclI T'GATC_A
Cuts at: 0 1412 1758
Size: 1412 346
BglI GCCn_nnn'nGGC
Cuts at: 0 44 1758
Size: 44 1714
BlpI GC'TnA_GC
Cuts at: 0 64 1758
Size: 64 1694
Bme1580I G_kGCm'C
Cuts at: 0 1567 1758
Size: 1567 191
BmrI ACTGGGnnnn_n'
Cuts at: 0 507 1758
Size: 507 1251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 178 811 1703 1758
Size: 73 105 633 892 55
Fragments arranged by size:
892 633 105 73 55
Bpu10I CC'TnA_GC
Cuts at: 0 528 1308 1758
Size: 528 780 450
Fragments arranged by size:
780 528 450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 835 1197 1440 1758
Size: 217 618 362 243 318
Fragments arranged by size:
618 362 318 243 217
BsaI GGTCTCn'nnnn_
Cuts at: 0 241 646 1758
Size: 241 405 1112
Fragments arranged by size:
1112 405 241
BsaAI yAC'GTr
Cuts at: 0 1228 1758
Size: 1228 530
BsaHI Gr'CG_yC
Cuts at: 0 389 699 1758
Size: 389 310 1059
Fragments arranged by size:
1059 389 310
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1598 1628 1676 1706 1758
Size: 1598 30 48 30 52
Fragments arranged by size:
1598 52 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 217 343 655 799 1624 1675 1678
Size: 115 102 126 312 144 825 51 3
Cuts at: 1678 1681 1758
Size: 3 77
Fragments arranged by size:
825 312 144 126 115 102 77 51
3 3
BseYI C'CCAG_C
Cuts at: 0 318 914 1403 1758
Size: 318 596 489 355
Fragments arranged by size:
596 489 355 318
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 1758
Size: 437 1321
BsiEI CG_ry'CG
Cuts at: 0 216 1758
Size: 216 1542
BsiHKAI G_wGCw'C
Cuts at: 0 116 224 1758
Size: 116 108 1534
Fragments arranged by size:
1534 116 108
BsmI GAATG_Cn'
Cuts at: 0 825 1758
Size: 825 933
BsmBI CGTCTCn'nnnn_
Cuts at: 0 154 1758
Size: 154 1604
Bsp1286I G_dGCh'C
Cuts at: 0 70 116 224 1567 1758
Size: 70 46 108 1343 191
Fragments arranged by size:
1343 191 108 70 46
BspHI T'CATG_A
Cuts at: 0 738 1758
Size: 738 1020
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 177 1758
Size: 177 1581
BsrFI r'CCGG_y
Cuts at: 0 122 226 1758
Size: 122 104 1532
Fragments arranged by size:
1532 122 104
BsrGI T'GTAC_A
Cuts at: 0 134 1758
Size: 134 1624
BssHII G'CGCG_C
Cuts at: 0 173 272 297 1758
Size: 173 99 25 1461
Fragments arranged by size:
1461 173 99 25
BssSI C'ACGA_G
Cuts at: 0 1557 1758
Size: 1557 201
BstAPI GCAn_nnn'nTGC
Cuts at: 0 445 1758
Size: 445 1313
BstBI TT'CG_AA
Cuts at: 0 430 1758
Size: 430 1328
BstYI r'GATC_y
Cuts at: 0 492 1758
Size: 492 1266
BtgI C'CryG_G
Cuts at: 0 25 1758
Size: 25 1733
DraI TTT'AAA
Cuts at: 0 1638 1758
Size: 1638 120
DraIII CAC_nnn'GTG
Cuts at: 0 460 1758
Size: 460 1298
DrdI GACnn_nn'nnGTC
Cuts at: 0 1435 1758
Size: 1435 323
EaeI y'GGCC_r
Cuts at: 0 213 257 1758
Size: 213 44 1501
Fragments arranged by size:
1501 213 44
EagI C'GGCC_G
Cuts at: 0 213 1758
Size: 213 1545
EarI CTCTTCn'nnn_
Cuts at: 0 632 1148 1243 1652 1758
Size: 632 516 95 409 106
Fragments arranged by size:
632 516 409 106 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 1054 1293 1758
Size: 349 705 239 465
Fragments arranged by size:
705 465 349 239
EcoICRI GAG'CTC
Cuts at: 0 114 222 1758
Size: 114 108 1536
Fragments arranged by size:
1536 114 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 178 517 811 1489 1673 1703 1758
Size: 73 105 339 294 678 184 30 55
Fragments arranged by size:
678 339 294 184 105 73 55 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 604 1758
Size: 604 1154
EcoRI G'AATT_C
Cuts at: 0 426 1758
Size: 426 1332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1199 1758
Size: 1199 559
FspI TGC'GCA
Cuts at: 0 151 1758
Size: 151 1607
HaeII r_GCGC'y
Cuts at: 0 241 392 1758
Size: 241 151 1366
Fragments arranged by size:
1366 241 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 1758
Size: 260 32 1466
Fragments arranged by size:
1466 260 32
HincII GTy'rAC
Cuts at: 0 858 1758
Size: 858 900
HindIII A'AGCT_T
Cuts at: 0 400 1758
Size: 400 1358
KasI G'GCGC_C
Cuts at: 0 388 1758
Size: 388 1370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1758
Size: 497 1261
MspA1I CmG'CkG
Cuts at: 0 27 63 183 348 1068 1681 1758
Size: 27 36 120 165 720 613 77
Fragments arranged by size:
720 613 165 120 77 36 27
NarI GG'CG_CC
Cuts at: 0 389 1758
Size: 389 1369
NspI r_CATG'y
Cuts at: 0 633 950 1014 1758
Size: 633 317 64 744
Fragments arranged by size:
744 633 317 64
PfoI T'CCnGG_A
Cuts at: 0 89 1758
Size: 89 1669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1540 1572 1758
Size: 1540 32 186
Fragments arranged by size:
1540 186 32
PsiI TTA'TAA
Cuts at: 0 1445 1758
Size: 1445 313
PstI C_TGCA'G
Cuts at: 0 182 188 311 537 1758
Size: 182 6 123 226 1221
Fragments arranged by size:
1221 226 182 123 6
PvuII CAG'CTG
Cuts at: 0 183 348 1068 1681 1758
Size: 183 165 720 613 77
Fragments arranged by size:
720 613 183 165 77
SacI G_AGCT'C
Cuts at: 0 116 224 1758
Size: 116 108 1534
Fragments arranged by size:
1534 116 108
SacII CC_GC'GG
Cuts at: 0 28 1758
Size: 28 1730
SfcI C'TryA_G
Cuts at: 0 178 184 307 533 928 1758
Size: 178 6 123 226 395 830
Fragments arranged by size:
830 395 226 178 123 6
SfoI GGC'GCC
Cuts at: 0 390 1758
Size: 390 1368
SmlI C'TyrA_G
Cuts at: 0 232 814 1212 1455 1758
Size: 232 582 398 243 303
Fragments arranged by size:
582 398 303 243 232
SnaBI TAC'GTA
Cuts at: 0 1228 1758
Size: 1228 530
SphI G_CATG'C
Cuts at: 0 1014 1758
Size: 1014 744
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 1758
Size: 265 1493
TatI w'GTAC_w
Cuts at: 0 134 716 1627 1758
Size: 134 582 911 131
Fragments arranged by size:
911 582 134 131
XbaI T'CTAG_A
Cuts at: 0 940 1758
Size: 940 818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1400 1758
Size: 1400 358
XmnI GAAnn'nnTTC
Cuts at: 0 959 1085 1758
Size: 959 126 673
Fragments arranged by size:
959 673 126
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI AvaI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI BtgI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI MmeI MspA1I NarI NspI
PfoI PpiI PsiI PstI PvuII SacI SacII SfcI
SfoI SmlI SnaBI SphI TaqII TatI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BfrBI BglII BmgBI BmtI
BplI BsaBI BsaWI BsiWI BspEI BsrBI BsrDI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI EcoO109I
EcoRV FseI FspAI HpaI KpnI MfeI MluI MscI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SpeI SrfI SspI StuI StyI SwaI TstI Tth111I
XhoI XmaI ZraI