################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AvrII BbrPI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BseAI BsiWI Bsp1407I BspDI BspEI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI MaeI MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI SexAI SfuI SnaBI SpeI SspBI SspI StuI SwaI Tru9I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 246 GACGTC GACGT^C Acc65I 697 GGTACC G^GTACC AflIII 1043 ACATGT A^C[GA][CT]GT AlwNI 310 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 28 GGGCCC GGGCC^C Asp718I 697 GGTACC G^GTACC AspHI 543 GAGCAC G[AT]GC[AT]^C AspI 750 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 134 TGCGCA TGC^GCA BamHI 948 GGATCC G^GATCC BanII 28 GGGCCC G[GA]GC[CT]^C BbvCI 276 CCTCAGC CCTCAGC(-5/-2) BcgI 125 CGAGGGACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 646 TACGTG [CT]AC^GT[GA] BsaWI 230 ACCGGA [AT]^CCGG[AT] BseRI 142 GAGGAG GAGGAG(10/8) BsiHKAI 543 GAGCAC G[AT]GC[AT]^C BsmAI 5 GTCTC GTCTC(1/5) BsmBI 4 CGTCTC CGTCTC(1/5) BsmI 301 GCATTC GAATGC(1/-1) BspLU11I 1043 ACATGT A^CATGT BssHII 490 GCGCGC G^CGCGC BssSI 638 CTCGTG CACGAG(-5/-1) DraIII 818 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 515 CGGCCG C^GGCCG EarI 262 CTCTTC CTCTTC(1/4) EclXI 515 CGGCCG C^GGCCG Eco52I 515 CGGCCG C^GGCCG EcoNI 102 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 134 TGCGCA TGC^GCA HindIII 1083 AAGCTT A^AGCTT KpnI 697 GGTACC GGTAC^C Ksp632I 262 CTCTTC CTCTTC(1/4) NcoI 49 CCATGG C^CATGG NotI 514 GCGGCCGC GC^GGCCGC PflFI 750 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 44 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 388 CTGCAG CTGCA^G SbfI 387 CCTGCAGG CCTGCA^GG ScaI 554 AGTACT AGT^ACT SfaNI 668 GCATC GCATC(5/9) SfcI 388 CTGCAG C^T[GA][CT]AG SfiI 285 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 66 CACCGGCG C[GA]^CCGG[CT]G SmaI 848 CCCGGG CCC^GGG SmlI 469 CTCAAG C^T[CT][GA]AG SphI 254 GCATGC GCATG^C Sse8387I 387 CCTGCAGG CCTGCA^GG TfiI 970 GAATC G^A[AT]TC TspRI 996 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 750 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 44 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 848 CCCGGG C^CCGGG XmaI 848 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcsI 1114 AAATTC [GA]^AATT[CT] 1234 AAATTC ApoI 1114 AAATTC [GA]^AATT[CT] 1234 AAATTC AvaII 16 GGTCC G^G[AT]CC 965 GGACC BbeI 678 GGCGCC GGCGC^C 912 GGCGCC BglI 166 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 286 GCCGACCTGGC BlpI 528 GCTGAGC GC^T[ACGT]AGC 540 GCTGAGC BmyI 28 GGGCCC G[AGT]GC[ACT]^C 543 GAGCAC BpmI 42 CTCCAG CTGGAG(16/14) 157 CTGGAG Bpu1102I 528 GCTGAGC GC^T[ACGT]AGC 540 GCTGAGC BsgI 226 CTGCAC GTGCAG(16/14) 773 GTGCAG BsiEI 441 CGACCG CG[GA][CT]^CG 515 CGGCCG Bsp1286I 28 GGGCCC G[AGT]GC[ACT]^C 543 GAGCAC BspHI 431 TCATGA T^CATGA 506 TCATGA BsrBI 466 CCGCTC CCGCTC(-3/-3) 495 CCGCTC BsrFI 13 GCCGGT [GA]^CCGG[CT] 67 ACCGGC BstYI 857 AGATCC [GA]^GATC[CT] 948 GGATCC CelII 528 GCTGAGC GC^T[ACGT]AGC 540 GCTGAGC Cfr10I 13 GCCGGT [GA]^CCGG[CT] 67 ACCGGC DraII 27 GGGGCCC [GA]G^G[ACGT]CC[CT] 28 GGGCCCT EcoO109I 27 GGGGCCC [GA]G^G[ACGT]CC[CT] 28 GGGCCCT EcoT14I 49 CCATGG C^C[AT][AT]GG 593 CCAAGG KasI 678 GGCGCC G^GCGCC 912 GGCGCC KspI 351 CCGCGG CCGC^GG 741 CCGCGG MflI 857 AGATCC [GA]^GATC[CT] 948 GGATCC MspA1I 351 CCGCGG C[AC]G^C[GT]G 741 CCGCGG NarI 678 GGCGCC GG^CGCC 912 GGCGCC PleI 474 GACTC GAGTC(4/5) 1207 GACTC RcaI 431 TCATGA T^CATGA 506 TCATGA SacII 351 CCGCGG CCGC^GG 741 CCGCGG SfoI 678 GGCGCC GGC^GCC 912 GGCGCC StyI 49 CCATGG C^C[AT][AT]GG 593 CCAAGG TaqI 575 TCGA T^CGA 1173 TCGA Tsp509I 1115 AATT ^AATT 1235 AATT TthHB8I 575 TCGA T^CGA 1173 TCGA XhoII 857 AGATCC [GA]^GATC[CT] 948 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaI BalI BanI BbsI BbvI BcnI BpuAI BsaHI BsaJI BsiYI BslI BsmFI BsoBI BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI TseI Tsp45I