(Circular) (Six-base) MAPSORT of: pF1KB4809.seq Check: 3933 from: 1 to: 5315 pF1KB4809 5315 bp With 182 enzymes: SgfI * June 20, 2008 10:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 401 401 Size: 5315 AccI GT'mk_AC Cuts at: 1313 2353 1313 Size: 1040 4275 Acc65I G'GTAC_C Cuts at: 860 2071 2331 860 Size: 1211 260 3844 Fragments arranged by size: 3844 1211 260 AclI AA'CG_TT Cuts at: 5155 5155 Size: 5315 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 547 808 1300 1804 1873 3140 3572 3925 4587 Size: 261 492 504 69 1267 432 353 662 Cuts at: 4587 547 Size: 1275 Fragments arranged by size: 1275 1267 662 504 492 432 353 261 69 AflIII A'CryG_T Cuts at: 1316 2667 4467 4748 1316 Size: 1351 1800 281 1883 Fragments arranged by size: 1883 1800 1351 281 AhdI GACnn_n'nnGTC Cuts at: 540 1374 540 Size: 834 4481 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1054 1086 1054 Size: 32 5283 AlwNI CAG_nnn'CTG Cuts at: 402 786 801 1278 1840 2620 4058 402 Size: 384 15 477 562 780 1438 1659 Fragments arranged by size: 1659 1438 780 562 477 384 15 ApaI G_GGCC'C Cuts at: 1887 1887 Size: 5315 ApaLI G'TGCA_C Cuts at: 4153 4153 Size: 5315 ApoI r'AATT_y Cuts at: 1383 2319 4668 4924 1383 Size: 936 2349 256 1774 Fragments arranged by size: 2349 1774 936 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5315 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5315 AvaI C'yCGr_G Cuts at: 670 2335 670 Size: 1665 3650 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 852 885 852 Size: 33 5282 BamHI G'GATC_C Cuts at: 2340 2340 Size: 5315 BanI G'GyrC_C Cuts at: 241 716 860 2071 2331 2745 2993 3028 241 Size: 475 144 1211 260 414 248 35 2528 Fragments arranged by size: 2528 1211 475 414 260 248 144 35 BanII G_rGCy'C Cuts at: 1054 1189 1887 2329 3359 1054 Size: 135 698 442 1030 3010 Fragments arranged by size: 3010 1030 698 442 135 BbeI G_GCGC'C Cuts at: 720 2749 2997 720 Size: 2029 248 3038 Fragments arranged by size: 3038 2029 248 BbvCI CC'TCA_GC Cuts at: 1975 1975 Size: 5315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 555 589 661 695 555 Size: 34 72 34 5175 Fragments arranged by size: 5175 72 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3213 4269 5315 3213 Size: 1056 1046 3213 Fragments arranged by size: 3213 1056 1046 BclI T'GATC_A Cuts at: 2835 2835 Size: 5315 BglI GCCn_nnn'nGGC Cuts at: 674 2008 674 Size: 1334 3981 BglII A'GATC_T Cuts at: 2830 4867 2830 Size: 2037 3278 BlpI GC'TnA_GC Cuts at: 2122 2427 2122 Size: 305 5010 Bme1580I G_kGCm'C Cuts at: 244 1147 1887 2940 3033 4157 244 Size: 903 740 1053 93 1124 1402 Fragments arranged by size: 1402 1124 1053 903 740 93 BmgBI CAC'GTC Cuts at: 1319 1319 Size: 5315 BmrI ACTGGGnnnn_n' Cuts at: 2527 2608 2943 5087 2527 Size: 81 335 2144 2755 Fragments arranged by size: 2755 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1128 3682 1128 Size: 2554 2761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 424 454 1902 1966 424 Size: 30 1448 64 3773 Fragments arranged by size: 3773 1448 64 30 Bpu10I CC'TnA_GC Cuts at: 1975 1975 Size: 5315 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 268 475 2128 2290 2491 3370 4084 4382 268 Size: 207 1653 162 201 879 714 298 1201 Fragments arranged by size: 1653 1201 879 714 298 207 201 162 BsaI GGTCTCn'nnnn_ Cuts at: 5019 5019 Size: 5315 BsaAI yAC'GTr Cuts at: 1728 2668 1728 Size: 940 4375 BsaBI GATnn'nnATC Cuts at: 6 1660 2829 4866 6 Size: 1654 1169 2037 455 Fragments arranged by size: 2037 1654 1169 455 BsaHI Gr'CG_yC Cuts at: 161 500 717 2746 2994 3696 5192 161 Size: 339 217 2029 248 702 1496 284 Fragments arranged by size: 2029 1496 702 339 284 248 217 BsaWI w'CCGG_w Cuts at: 2068 2504 3025 3703 4114 4261 4535 2068 Size: 436 521 678 411 147 274 2848 Fragments arranged by size: 2848 678 521 436 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1054 1084 1394 1424 1900 1930 1054 Size: 30 310 30 476 30 4439 Fragments arranged by size: 4439 476 310 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 475 1075 1078 1081 1238 1699 2035 475 Size: 600 3 3 157 461 336 3755 Fragments arranged by size: 3755 600 461 336 157 3 3 BseYI C'CCAG_C Cuts at: 712 1039 1271 1522 1589 2740 3498 4163 4571 Size: 327 232 251 67 1151 758 665 408 Cuts at: 4571 712 Size: 1456 Fragments arranged by size: 1456 1151 758 665 408 327 251 232 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1708 1708 Size: 5315 BsiEI CG_ry'CG Cuts at: 104 249 1587 2857 2903 4133 104 Size: 145 1338 1270 46 1230 1286 Fragments arranged by size: 1338 1286 1270 1230 145 46 BsiHKAI G_wGCw'C Cuts at: 392 470 1679 2183 2225 2329 3107 3297 4157 Size: 78 1209 504 42 104 778 190 860 Cuts at: 4157 392 Size: 1550 Fragments arranged by size: 1550 1209 860 778 504 190 104 78 42 BsmI GAATG_Cn' Cuts at: 515 515 Size: 5315 BsmBI CGTCTCn'nnnn_ Cuts at: 1591 1591 Size: 5315 Bsp1286I G_dGCh'C Cuts at: 244 392 470 1054 1147 1189 1679 1887 2183 Size: 148 78 584 93 42 490 208 296 Cuts at: 2183 2225 2329 2940 3033 3107 3297 3359 4157 Size: 42 104 611 93 74 190 62 798 Cuts at: 4157 244 Size: 1402 Fragments arranged by size: 1402 798 611 584 490 296 208 190 148 104 93 93 78 74 62 42 42 BspHI T'CATG_A Cuts at: 3747 3747 Size: 5315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 401 1244 2365 2579 2881 3262 401 Size: 843 1121 214 302 381 2454 Fragments arranged by size: 2454 1121 843 381 302 214 BsrBI CCG'CTC Cuts at: 50 1880 1916 3607 3661 5145 5310 50 Size: 1830 36 1691 54 1484 165 55 Fragments arranged by size: 1830 1691 1484 165 55 54 36 BsrDI GCAATG_nn' Cuts at: 650 1163 3227 650 Size: 513 2064 2738 Fragments arranged by size: 2738 2064 513 BsrFI r'CCGG_y Cuts at: 3313 3494 3313 Size: 181 5134 BsrGI T'GTAC_A Cuts at: 371 371 Size: 5315 BssSI C'ACGA_G Cuts at: 1894 3586 4294 1894 Size: 1692 708 2915 Fragments arranged by size: 2915 1692 708 BstAPI GCAn_nnn'nTGC Cuts at: 801 1840 2250 2280 2692 801 Size: 1039 410 30 412 3424 Fragments arranged by size: 3424 1039 412 410 30 BstBI TT'CG_AA Cuts at: 3676 3676 Size: 5315 BstEII G'GTnAC_C Cuts at: 435 894 435 Size: 459 4856 BstXI CCAn_nnnn'nTGG Cuts at: 1413 1413 Size: 5315 BstYI r'GATC_y Cuts at: 1 333 557 2267 2340 2807 2830 3165 3411 Size: 332 224 1710 73 467 23 335 246 Cuts at: 3411 3717 3729 3815 4867 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1710 1052 467 449 335 332 306 246 224 86 73 23 12 BstZ17I GTA'TAC Cuts at: 1314 1314 Size: 5315 BtgI C'CryG_G Cuts at: 819 1406 1490 819 Size: 587 84 4644 Fragments arranged by size: 4644 587 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 558 1107 1947 2260 3265 3446 3609 558 Size: 549 840 313 1005 181 163 2264 Fragments arranged by size: 2264 1005 840 549 313 181 163 BtsI GCAGTG_nn' Cuts at: 2558 2558 Size: 5315 DraI TTT'AAA Cuts at: 2314 2314 Size: 5315 DraIII CAC_nnn'GTG Cuts at: 552 2238 552 Size: 1686 3629 DrdI GACnn_nn'nnGTC Cuts at: 481 877 1266 3021 4365 481 Size: 396 389 1755 1344 1431 Fragments arranged by size: 1755 1431 1344 396 389 EaeI y'GGCC_r Cuts at: 338 359 1026 1415 1584 1703 1775 2900 3074 Size: 21 667 389 169 119 72 1125 174 Cuts at: 3074 3465 3492 4837 338 Size: 391 27 1345 816 Fragments arranged by size: 1345 1125 816 667 391 389 174 169 119 72 27 21 EagI C'GGCC_G Cuts at: 1584 2900 1584 Size: 1316 3999 EarI CTCTTCn'nnn_ Cuts at: 2041 3338 3548 2041 Size: 1297 210 3808 Fragments arranged by size: 3808 1297 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1415 3523 4265 4411 5125 1415 Size: 2108 742 146 714 1605 Fragments arranged by size: 2108 1605 742 714 146 EcoICRI GAG'CTC Cuts at: 2327 2327 Size: 5315 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 424 454 547 808 1300 1804 1873 1902 1966 Size: 30 93 261 492 504 69 29 64 Cuts at: 1966 3140 3572 3925 4587 424 Size: 1174 432 353 662 1152 Fragments arranged by size: 1174 1152 662 504 492 432 353 261 93 69 64 30 29 EcoO109I rG'GnC_Cy Cuts at: 354 1251 1368 1883 2025 2454 354 Size: 897 117 515 142 429 3215 Fragments arranged by size: 3215 897 515 429 142 117 EcoRI G'AATT_C Cuts at: 2319 2319 Size: 5315 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2169 3334 2169 Size: 1165 4150 FspI TGC'GCA Cuts at: 1960 3096 1960 Size: 1136 4179 FspAI rTGC'GCAy Cuts at: 1960 1960 Size: 5315 HaeII r_GCGC'y Cuts at: 210 720 2151 2749 2997 4227 4998 210 Size: 510 1431 598 248 1230 771 527 Fragments arranged by size: 1431 1230 771 598 527 510 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 318 350 526 558 1214 1246 1360 1392 1394 Size: 32 176 32 656 32 114 32 2 Cuts at: 1394 1426 1900 1932 2259 2291 3157 3187 3189 Size: 32 474 32 327 32 866 30 2 Cuts at: 3189 3219 318 Size: 30 2414 Fragments arranged by size: 2414 866 656 474 327 176 114 32 32 32 32 32 32 32 30 30 2 2 HincII GTy'rAC Cuts at: 2354 2354 Size: 5315 KasI G'GCGC_C Cuts at: 716 2745 2993 716 Size: 2029 248 3038 Fragments arranged by size: 3038 2029 248 KpnI G_GTAC'C Cuts at: 864 2075 2335 864 Size: 1211 260 3844 Fragments arranged by size: 3844 1211 260 MluI A'CGCG_T Cuts at: 4748 4748 Size: 5315 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 476 512 2033 4074 4258 476 Size: 36 1521 2041 184 1533 Fragments arranged by size: 2041 1533 1521 184 36 MscI TGG'CCA Cuts at: 340 1705 1777 3076 340 Size: 1365 72 1299 2579 Fragments arranged by size: 2579 1365 1299 72 MslI CAynn'nnrTG Cuts at: 655 1006 1150 1870 1933 3304 3431 655 Size: 351 144 720 63 1371 127 2539 Fragments arranged by size: 2539 1371 720 351 144 127 63 MspA1I CmG'CkG Cuts at: 198 399 629 1342 1550 1589 1944 2426 2740 Size: 201 230 713 208 39 355 482 314 Cuts at: 2740 3100 3884 4129 198 Size: 360 784 245 1384 Fragments arranged by size: 1384 784 713 482 360 355 314 245 230 208 201 39 NaeI GCC'GGC Cuts at: 3496 3496 Size: 5315 NarI GG'CG_CC Cuts at: 717 2746 2994 717 Size: 2029 248 3038 Fragments arranged by size: 3038 2029 248 NcoI C'CATG_G Cuts at: 1406 1490 1406 Size: 84 5231 NgoMIV G'CCGG_C Cuts at: 3494 3494 Size: 5315 NspI r_CATG'y Cuts at: 843 1714 2283 2368 2868 4471 843 Size: 871 569 85 500 1603 1687 Fragments arranged by size: 1687 1603 871 569 500 85 PasI CC'CwG_GG Cuts at: 883 1255 883 Size: 372 4943 PciI A'CATG_T Cuts at: 4467 4467 Size: 5315 PfoI T'CCnGG_A Cuts at: 470 1378 470 Size: 908 4407 PmeI GTTT'AAAC Cuts at: 2314 2314 Size: 5315 PmlI CAC'GTG Cuts at: 1728 1728 Size: 5315 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1054 1086 3761 3793 1054 Size: 32 2675 32 2576 Fragments arranged by size: 2675 2576 32 32 PpuMI rG'GwC_Cy Cuts at: 354 1251 1368 354 Size: 897 117 4301 Fragments arranged by size: 4301 897 117 PspOMI G'GGCC_C Cuts at: 1883 1883 Size: 5315 PspXI vC'TCGA_Gb Cuts at: 670 670 Size: 5315 PstI C_TGCA'G Cuts at: 203 398 797 1845 1994 2246 2362 3047 203 Size: 195 399 1048 149 252 116 685 2471 Fragments arranged by size: 2471 1048 685 399 252 195 149 116 PvuI CG_AT'CG Cuts at: 104 104 Size: 5315 PvuII CAG'CTG Cuts at: 198 399 629 1342 1944 2740 3100 198 Size: 201 230 713 602 796 360 2413 Fragments arranged by size: 2413 796 713 602 360 230 201 RsrII CG'GwC_CG Cuts at: 588 3510 588 Size: 2922 2393 SacI G_AGCT'C Cuts at: 2329 2329 Size: 5315 SalI G'TCGA_C Cuts at: 2352 2352 Size: 5315 SanDI GG'GwC_CC Cuts at: 1251 1368 1251 Size: 117 5198 SapI GCTCTTCn'nnn_ Cuts at: 2041 3338 3548 2041 Size: 1297 210 3808 Fragments arranged by size: 3808 1297 210 SbfI CC_TGCA'GG Cuts at: 2362 2362 Size: 5315 ScaI AGT'ACT Cuts at: 310 4832 310 Size: 4522 793 SexAI A'CCwGG_T Cuts at: 548 548 Size: 5315 SfcI C'TryA_G Cuts at: 33 138 199 394 793 1841 1990 2242 2358 Size: 105 61 195 399 1048 149 252 116 Cuts at: 2358 3043 4011 4202 33 Size: 685 968 191 1146 Fragments arranged by size: 1146 1048 968 685 399 252 195 191 149 116 105 61 SfoI GGC'GCC Cuts at: 718 2747 2995 718 Size: 2029 248 3038 Fragments arranged by size: 3038 2029 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5315 SmaI CCC'GGG Cuts at: 2337 2337 Size: 5315 SmlI C'TyrA_G Cuts at: 283 490 670 2143 2305 2470 3385 4099 4361 Size: 207 180 1473 162 165 915 714 262 Cuts at: 4361 283 Size: 1237 Fragments arranged by size: 1473 1237 915 714 262 207 180 165 162 SpeI A'CTAG_T Cuts at: 67 67 Size: 5315 SphI G_CATG'C Cuts at: 2283 2368 2868 2283 Size: 85 500 4730 Fragments arranged by size: 4730 500 85 StyI C'CwwG_G Cuts at: 416 596 1406 1490 1517 2449 2803 416 Size: 180 810 84 27 932 354 2928 Fragments arranged by size: 2928 932 810 354 180 84 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2898 3031 3490 3699 2898 Size: 133 459 209 4514 Fragments arranged by size: 4514 459 209 133 TatI w'GTAC_w Cuts at: 308 371 4830 308 Size: 63 4459 793 Fragments arranged by size: 4459 793 63 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4832 4864 4832 Size: 32 5283 Tth111I GACn'n_nGTC Cuts at: 874 3112 874 Size: 2238 3077 XbaI T'CTAG_A Cuts at: 2346 2346 Size: 5315 XcmI CCAnnnn_n'nnnnTGG Cuts at: 729 2810 729 Size: 2081 3234 XhoI C'TCGA_G Cuts at: 670 670 Size: 5315 XmaI C'CCGG_G Cuts at: 2335 2335 Size: 5315 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AfeI AflII AgeI AleI AlfI AscI AvrII BbsI BfrBI BmtI BsiWI BspEI BssHII Bsu36I ClaI CspCI EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PshAI PsiI PsrI SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI