Restriction map for pF1KA0078
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# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AvaI        
AviII       AvrII       BbeI        BbrPI       BcgI        
BciVI       BcnI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmFI       
BsoBI       Bsp1407I    BspEI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstXI       
BstZ17I     CfoI        Cfr10I      CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
FauI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MfeI        MluI        MroI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NotI        
NruI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        XbaI        XcmI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflIII        542  ACATGT               A^C[GA][CT]GT
Asp700I       334  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          979  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI           41  TGGCCA               TGG^CCA
BamHI         843  GGATCC               G^GATCC
BanI         1059  GGCACC               G^G[CT][GA]CC
BbvCI        1471  CCTCAGC              CCTCAGC(-5/-2)
BlpI         1129  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1129  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1642  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI        1876  CCAAGG               C^C[ACGT][ACGT]GG
BsgI          776  CTGCAC               GTGCAG(16/14)
BsmAI        1093  GAGAC                GTCTC(1/5)
BsmI          241  GCATTC               GAATGC(1/-1)
BspHI         342  TCATGA               T^CATGA
BspLU11I      542  ACATGT               A^CATGT
BstBI         191  TTCGAA               TT^CGAA
CelII        1129  GCTCAGC              GC^T[ACGT]AGC
DraII          33  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I       33  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1243  GAATTC               G^AATTC
EcoT14I      1876  CCAAGG               C^C[AT][AT]GG
EcoT22I      1628  ATGCAT               ATGCA^T
MamI         1642  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI          41  TGGCCA               TGG^CCA
MscI           41  TGGCCA               TGG^CCA
MslI          378  CATCGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI           54  GCTAGC               G^CTAGC
NsiI         1628  ATGCAT               ATGCA^T
NspI          542  ACATGT               [GA]CATG^[CT]
NspV          191  TTCGAA               TT^CGAA
PflFI         979  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          44  CCAAAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          342  TCATGA               T^CATGA
SapI         1336  GAAGAGC              GCTCTTC(1/4)
SfuI          191  TTCGAA               TT^CGAA
SmlI         1744  CTTGAG               C^T[CT][GA]AG
SspI          681  AATATT               AAT^ATT
StyI         1876  CCAAGG               C^C[AT][AT]GG
Tsp45I       1853  GTGAC                ^GT[GC]AC
Tth111I       979  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         44  CCAAAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          334  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AfaI         1189  GTAC                 GT^AC
             1848  GTAC                
AlwNI         355  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1454  CAGAGCCTG           
AocI          289  CCTGAGG              CC^T[ACGT]AGG
             1417  CCTCAGG             
AspHI         591  GAGCAC               G[AT]GC[AT]^C
             1706  GTGCTC              
AvaII        1284  GGACC                G^G[AT]CC
             1873  GGACC               
BanII        1013  GAGCCC               G[GA]GC[CT]^C
             1324  GAGCCC              
BbsI         1195  GAAGAC               GAAGAC(2/6)
             1697  GTCTTC              
BclI          351  TGATCA               T^GATCA
              873  TGATCA              
BfaI           55  CTAG                 C^TAG
             1277  CTAG                
BpmI         1351  CTCCAG               CTGGAG(16/14)
             1721  CTGGAG              
BpuAI        1195  GAAGAC               GAAGAC(2/6)
             1697  GTCTTC              
BsiHKAI       591  GAGCAC               G[AT]GC[AT]^C
             1706  GTGCTC              
BspDI         379  ATCGAT               AT^CGAT
             1318  ATCGAT              
BsrI          180  ACTGG                ACTGG(1/-1)
             1720  ACTGG               
BstYI         843  GGATCC               [GA]^GATC[CT]
             1053  GGATCT              
Bsu36I        289  CCTGAGG              CC^T[ACGT]AGG
             1417  CCTCAGG             
ClaI          379  ATCGAT               AT^CGAT
             1318  ATCGAT              
EaeI           41  TGGCCA               [CT]^GGCC[GA]
              268  CGGCCA              
Eco81I        289  CCTGAGG              CC^T[ACGT]AGG
             1417  CCTCAGG             
EcoNI         285  CCTGCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              741  CCTCTCTGAGG         
FbaI          351  TGATCA               T^GATCA
              873  TGATCA              
HincII        280  GTTGAC               GT[CT]^[GA]AC
              976  GTTGAC              
HindII        280  GTTGAC               GT[CT]^[GA]AC
              976  GTTGAC              
MaeI           55  CTAG                 C^TAG
             1277  CTAG                
MaeIII       1042  GTTAC                ^GT[ACGT]AC
             1853  GTGAC               
MflI          843  GGATCC               [GA]^GATC[CT]
             1053  GGATCT              
RsaI         1189  GTAC                 GT^AC
             1848  GTAC                
SfcI         1502  CTGTAG               C^T[GA][CT]AG
             1791  CTACAG              
XhoII         843  GGATCC               [GA]^GATC[CT]
             1053  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BbvI        BmyI        BseRI       BsiYI       BslI        
Bsp1286I    BspMI       BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MboI        MboII       
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     
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