Restriction map for pF1KA0078
 (Linear) (Six-base) MAPSORT of: KIAA0078.seq  Check: 5462  from: 1  to: 1893



KIAA0078.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353    1177    1684    1893 
   Size:        353     824     507     209

  Fragments arranged by size: 

                 824     507     353     209
 
AflIII A'CryG_T

Cuts at:      0     542    1893 
   Size:        542    1351
 
AlwNI CAG_nnn'CTG

Cuts at:      0     360    1459    1893 
   Size:        360    1099     434

  Fragments arranged by size: 

                1099     434     360
 
ApoI r'AATT_y

Cuts at:      0      47     337    1243    1255    1893 
   Size:         47     290     906      12     638

  Fragments arranged by size: 

                 906     638     290      47      12
 
BamHI G'GATC_C

Cuts at:      0     843    1893 
   Size:        843    1050
 
BanI G'GyrC_C

Cuts at:      0    1059    1893 
   Size:       1059     834
 
BanII G_rGCy'C

Cuts at:      0    1017    1328    1893 
   Size:       1017     311     565

  Fragments arranged by size: 

                1017     565     311
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1202    1690    1893 
   Size:       1202     488     203

  Fragments arranged by size: 

                1202     488     203
 
BbvCI CC'TCA_GC

Cuts at:      0    1472    1893 
   Size:       1472     421
 
BclI T'GATC_A

Cuts at:      0     351     873    1893 
   Size:        351     522    1020

  Fragments arranged by size: 

                1020     522     351
 
BfrBI ATG'CAT

Cuts at:      0    1630    1893 
   Size:       1630     263
 
BlpI GC'TnA_GC

Cuts at:      0    1130    1893 
   Size:       1130     763
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     190    1893 
   Size:        190    1703
 
BmtI G_CTAG'C

Cuts at:      0      58    1893 
   Size:         58    1835
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1336    1742    1893 
   Size:       1336     406     151

  Fragments arranged by size: 

                1336     406     151
 
Bpu10I CC'TnA_GC

Cuts at:      0    1472    1893 
   Size:       1472     421
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1765    1893 
   Size:       1765     128
 
BsaBI GATnn'nnATC

Cuts at:      0    1646    1893 
   Size:       1646     247
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1115    1235    1460    1893 
   Size:       1115     120     225     433

  Fragments arranged by size: 

                1115     433     225     120
 
BseYI C'CCAG_C

Cuts at:      0     389    1893 
   Size:        389    1504
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     761    1893 
   Size:        761    1132
 
BsiHKAI G_wGCw'C

Cuts at:      0     595    1710    1893 
   Size:        595    1115     183

  Fragments arranged by size: 

                1115     595     183
 
BsmI GAATG_Cn'

Cuts at:      0     241    1893 
   Size:        241    1652
 
Bsp1286I G_dGCh'C

Cuts at:      0     595    1017    1328    1710    1893 
   Size:        595     422     311     382     183

  Fragments arranged by size: 

                 595     422     382     311     183
 
BspHI T'CATG_A

Cuts at:      0     342    1893 
   Size:        342    1551
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     293    1134    1467    1893 
   Size:        293     841     333     426

  Fragments arranged by size: 

                 841     426     333     293
 
BstBI TT'CG_AA

Cuts at:      0     192    1893 
   Size:        192    1701
 
BstYI r'GATC_y

Cuts at:      0     843    1053    1893 
   Size:        843     210     840

  Fragments arranged by size: 

                 843     840     210
 
Bsu36I CC'TnA_GG

Cuts at:      0     290    1418    1893 
   Size:        290    1128     475

  Fragments arranged by size: 

                1128     475     290
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1845    1893 
   Size:       1845      48
 
BtsI GCAGTG_nn'

Cuts at:      0     358     528    1893 
   Size:        358     170    1365

  Fragments arranged by size: 

                1365     358     170
 
ClaI AT'CG_AT

Cuts at:      0     380    1319    1893 
   Size:        380     939     574

  Fragments arranged by size: 

                 939     574     380
 
EaeI y'GGCC_r

Cuts at:      0      41     268    1893 
   Size:         41     227    1625

  Fragments arranged by size: 

                1625     227      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     416     955    1331    1604    1610    1893 
   Size:        416     539     376     273       6     283

  Fragments arranged by size: 

                 539     416     376     283     273       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353    1177    1336    1684    1742    1893 
   Size:        353     824     159     348      58     151

  Fragments arranged by size: 

                 824     353     348     159     151      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     289     745    1893 
   Size:        289     456    1148

  Fragments arranged by size: 

                1148     456     289
 
EcoO109I rG'GnC_Cy

Cuts at:      0      34    1893 
   Size:         34    1859
 
EcoRI G'AATT_C

Cuts at:      0    1243    1893 
   Size:       1243     650
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     793     825    1455    1487    1893 
   Size:        793      32     630      32     406

  Fragments arranged by size: 

                 793     630     406      32      32
 
HincII GTy'rAC

Cuts at:      0     282     978    1893 
   Size:        282     696     915

  Fragments arranged by size: 

                 915     696     282
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    1893 
   Size:        974     919
 
MscI TGG'CCA

Cuts at:      0      43    1893 
   Size:         43    1850
 
MslI CAynn'nnrTG

Cuts at:      0     382    1893 
   Size:        382    1511
 
NheI G'CTAG_C

Cuts at:      0      54    1893 
   Size:         54    1839
 
NsiI A_TGCA'T

Cuts at:      0    1632    1893 
   Size:       1632     261
 
NspI r_CATG'y

Cuts at:      0     546    1893 
   Size:        546    1347
 
PciI A'CATG_T

Cuts at:      0     542    1893 
   Size:        542    1351
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      50    1893 
   Size:         50    1843
 
PfoI T'CCnGG_A

Cuts at:      0    1352    1893 
   Size:       1352     541
 
PsiI TTA'TAA

Cuts at:      0     314    1893 
   Size:        314    1579
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1331    1893 
   Size:       1331     562
 
SfcI C'TryA_G

Cuts at:      0    1502    1791    1893 
   Size:       1502     289     102

  Fragments arranged by size: 

                1502     289     102
 
SmlI C'TyrA_G

Cuts at:      0    1744    1893 
   Size:       1744     149
 
SspI AAT'ATT

Cuts at:      0     683    1893 
   Size:        683    1210
 
StyI C'CwwG_G

Cuts at:      0    1876    1893 
   Size:       1876      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     805    1893 
   Size:        805    1088
 
Tth111I GACn'n_nGTC

Cuts at:      0     982    1893 
   Size:        982     911
 
XmnI GAAnn'nnTTC

Cuts at:      0     338    1893 
   Size:        338    1555
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI    BamHI     BanI    BanII     BbsI
    BbvCI     BclI    BfrBI     BlpI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI    BsaBI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BstBI    BstYI   Bsu36I    BtgZI     BtsI     ClaI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I   HincII
     MmeI     MscI     MslI     NheI     NsiI     NspI     PciI    PflMI
     PfoI     PsiI     SapI     SfcI     SmlI     SspI     StyI    TaqII
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BbeI     BcgI    BciVI     BglI
    BglII Bme1580I    BmgBI     BplI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I     BtgI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     PacI     PasI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI
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