Restriction map for pF1KB9467
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccIII      AgeI        AhdI        
AocI        ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BlnI        BlpI        Bpu1102I    BsaAI       
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BspHI       BsrBI       BssHII      BssSI       BstAPI      
BstBI       Bsu36I      CelII       ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoT22I     
FauI        FseI        FspI        KasI        KpnI        
KspI        MluI        MroI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII          24  CGCG                 CG^CG
AclI         3261  AACGTT               AA^CGTT
AcyI         2715  GGCGTC               G[GA]^CG[CT]C
AflII        2736  CTTAAG               C^TTAAG
AlwNI         797  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        51  AGCGCT               AGC^GCT
Asp700I      3152  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1816  GGATCC               G^GATCC
BclI          888  TGATCA               T^GATCA
BfrI         2736  CTTAAG               C^TTAAG
BglI         1634  GCCCCGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2824  AGATCT               A^GATCT
BsaHI        2715  GGCGTC               G[GA]^CG[CT]C
BsaWI         121  TCCGGT               [AT]^CCGG[AT]
BsmBI        2717  CGTCTC               CGTCTC(1/5)
BsrDI         411  GCAATG               GCAATG(2/0)
BstEII       1450  GGTGACC              G^GT[ACGT]ACC
BstPI        1450  GGTGACC              G^GT[ACGT]ACC
BstUI          24  CGCG                 CG^CG
Eco47III       51  AGCGCT               AGC^GCT
EcoO65I      1450  GGTGACC              G^GT[ACGT]ACC
EcoRI        1743  GAATTC               G^AATTC
EcoRV        3064  GATATC               GAT^ATC
FbaI          888  TGATCA               T^GATCA
HaeII          51  AGCGCT               [GA]GCGC^[CT]
HgaI         2716  GCGTC                GACGC(5/10)
Hin1I        2715  GGCGTC               G[GA]^CG[CT]C
HindIII      1973  AAGCTT               A^AGCTT
HpaI         2466  GTTAAC               GTT^AAC
MfeI         1670  CAATTG               C^AATTG
MunI         1670  CAATTG               C^AATTG
MvnI           24  CGCG                 CG^CG
PflMI        3230  CCAGAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1577  GAGTC                GAGTC(4/5)
Psp1406I     3261  AACGTT               AA^CGTT
SacI         3532  GAGCTC               GAGCT^C
ScaI         1847  AGTACT               AGT^ACT
SspI          114  AATATT               AAT^ATT
StuI          600  AGGCCT               AGG^CCT
SwaI         1880  ATTTAAAT             ATTT^AAAT
TaqI         1656  TCGA                 T^CGA
TthHB8I      1656  TCGA                 T^CGA
Van91I       3230  CCAGAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         3152  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AflIII        271  ACATGT               A^C[GA][CT]GT
              388  ACATGT              
Alw44I       1535  GTGCAC               G^TGCAC
             2881  GTGCAC              
ApaLI        1535  GTGCAC               G^TGCAC
             2881  GTGCAC              
BalI         1242  TGGCCA               TGG^CCA
             2081  TGGCCA              
BanI         1915  GGTGCC               G^G[CT][GA]CC
             2168  GGTGCC              
BanII        2236  GAGCCC               G[GA]GC[CT]^C
             3532  GAGCTC              
BbsI         1230  GAAGAC               GAAGAC(2/6)
             2338  GTCTTC              
BpuAI        1230  GAAGAC               GAAGAC(2/6)
             2338  GTCTTC              
BsaBI        2117  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3064  GATATCAATC          
BsaI         3523  GAGACC               GGTCTC(1/5)
               97  GGTCTC              
BseRI        1367  CTCCTC               GAGGAG(10/8)
             1109  GAGGAG              
BsmI         2013  GAATGC               GAATGC(1/-1)
              305  GCATTC              
Bsp1407I      594  TGTACA               T^GTACA
             1544  TGTACA              
BspLU11I      271  ACATGT               A^CATGT
              388  ACATGT              
BspMI         489  ACCTGC               ACCTGC(4/8)
             3175  ACCTGC              
BsrFI        1384  ACCGGC               [GA]^CCGG[CT]
             2568  GCCGGT              
BsrGI         594  TGTACA               T^GTACA
             1544  TGTACA              
Bst1107I     1756  GTATAC               GTA^TAC
             2985  GTATAC              
BstYI        1816  GGATCC               [GA]^GATC[CT]
             2824  AGATCT              
BstZ17I      1756  GTATAC               GTA^TAC
             2985  GTATAC              
CfoI           25  GCGC                 GCG^C
               52  GCGC                
Cfr10I       1384  ACCGGC               [GA]^CCGG[CT]
             2568  GCCGGT              
HhaI           25  GCGC                 GCG^C
               52  GCGC                
HinP1I         25  GCGC                 G^CGC
               52  GCGC                
MamI         2117  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3064  GATATCAATC          
MflI         1816  GGATCC               [GA]^GATC[CT]
             2824  AGATCT              
MluNI        1242  TGGCCA               TGG^CCA
             2081  TGGCCA              
MscI         1242  TGGCCA               TGG^CCA
             2081  TGGCCA              
PpuMI         740  GGGACCT              [GA]G^G[AT]CC[CT]
             1858  AGGTCCT             
PvuII        2420  CAGCTG               CAG^CTG
             3287  CAGCTG              
SmlI         2426  CTCAAG               C^T[CT][GA]AG
             2736  CTTAAG              
SspBI         594  TGTACA               T^GTACA
             1544  TGTACA              
XcmI          172  CCAAAAGGCAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2355  CCACAAAGACTGTGG     
XhoII        1816  GGATCC               [GA]^GATC[CT]
             2824  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaI        AvaII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PshAI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com