Restriction map for
pF1KB9467
(Linear) (Six-base) MAPSORT of: KIBB9467.seq Check: 198 from: 1 to: 3543
KIBB9467.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 498 3543
Size: 498 3045
AccI GT'mk_AC
Cuts at: 0 1580 1757 2986 3543
Size: 1580 177 1229 557
Fragments arranged by size:
1580 1229 557 177
AclI AA'CG_TT
Cuts at: 0 3262 3543
Size: 3262 281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2025 2325 2689 2944 3543
Size: 2025 300 364 255 599
Fragments arranged by size:
2025 599 364 300 255
AfeI AGC'GCT
Cuts at: 0 53 3543
Size: 53 3490
AflII C'TTAA_G
Cuts at: 0 2736 3543
Size: 2736 807
AflIII A'CryG_T
Cuts at: 0 271 388 3543
Size: 271 117 3155
Fragments arranged by size:
3155 271 117
AleI CACnn'nnGTG
Cuts at: 0 1448 1903 3543
Size: 1448 455 1640
Fragments arranged by size:
1640 1448 455
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2223 3543
Size: 2223 1320
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1916 1948 3543
Size: 1916 32 1595
Fragments arranged by size:
1916 1595 32
AlwNI CAG_nnn'CTG
Cuts at: 0 802 3543
Size: 802 2741
ApaLI G'TGCA_C
Cuts at: 0 1535 2881 3543
Size: 1535 1346 662
Fragments arranged by size:
1535 1346 662
ApoI r'AATT_y
Cuts at: 0 285 576 589 969 1079 1277 1743 2786
Size: 285 291 13 380 110 198 466 1043
Cuts at: 2786 2810 2838 2908 3120 3233 3543
Size: 24 28 70 212 113 310
Fragments arranged by size:
1043 466 380 310 291 285 212 198
113 110 70 28 24 13
AvaI C'yCGr_G
Cuts at: 0 1370 1636 2212 3543
Size: 1370 266 576 1331
Fragments arranged by size:
1370 1331 576 266
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2995 3028 3543
Size: 2995 33 515
Fragments arranged by size:
2995 515 33
BamHI G'GATC_C
Cuts at: 0 1816 3543
Size: 1816 1727
BanI G'GyrC_C
Cuts at: 0 1915 2168 3543
Size: 1915 253 1375
Fragments arranged by size:
1915 1375 253
BanII G_rGCy'C
Cuts at: 0 2240 3536 3543
Size: 2240 1296 7
Fragments arranged by size:
2240 1296 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1237 2331 3543
Size: 1237 1094 1212
Fragments arranged by size:
1237 1212 1094
BclI T'GATC_A
Cuts at: 0 888 3543
Size: 888 2655
BglI GCCn_nnn'nGGC
Cuts at: 0 1640 3543
Size: 1640 1903
BglII A'GATC_T
Cuts at: 0 2824 3543
Size: 2824 719
Bme1580I G_kGCm'C
Cuts at: 0 408 1539 1783 2885 3543
Size: 408 1131 244 1102 658
Fragments arranged by size:
1131 1102 658 408 244
BmgBI CAC'GTC
Cuts at: 0 1521 3543
Size: 1521 2022
BmrI ACTGGGnnnn_n'
Cuts at: 0 3029 3543
Size: 3029 514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 400 3543
Size: 400 3143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 395 1230 1358 1769 1828 2121 3543
Size: 225 170 835 128 411 59 293 1422
Fragments arranged by size:
1422 835 411 293 225 170 128 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2411 3543
Size: 2411 1132
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 3517 3543
Size: 103 3414 26
Fragments arranged by size:
3414 103 26
BsaBI GATnn'nnATC
Cuts at: 0 2121 3068 3543
Size: 2121 947 475
Fragments arranged by size:
2121 947 475
BsaHI Gr'CG_yC
Cuts at: 0 2716 3543
Size: 2716 827
BsaWI w'CCGG_w
Cuts at: 0 121 3543
Size: 121 3422
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1124 1358 3543
Size: 1124 234 2185
Fragments arranged by size:
2185 1124 234
BseYI C'CCAG_C
Cuts at: 0 1060 1185 3543
Size: 1060 125 2358
Fragments arranged by size:
2358 1060 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1172 1211 3543
Size: 175 997 39 2332
Fragments arranged by size:
2332 997 175 39
BsiHKAI G_wGCw'C
Cuts at: 0 1368 1526 1539 2219 2885 3536 3543
Size: 1368 158 13 680 666 651 7
Fragments arranged by size:
1368 680 666 651 158 13 7
BsmI GAATG_Cn'
Cuts at: 0 305 2019 3543
Size: 305 1714 1524
Fragments arranged by size:
1714 1524 305
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2723 3543
Size: 2723 820
Bsp1286I G_dGCh'C
Cuts at: 0 408 1368 1526 1539 1783 2219 2240 2885
Size: 408 960 158 13 244 436 21 645
Cuts at: 2885 3536 3543
Size: 651 7
Fragments arranged by size:
960 651 645 436 408 244 158 21
13 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 498 3184 3543
Size: 498 2686 359
Fragments arranged by size:
2686 498 359
BsrDI GCAATG_nn'
Cuts at: 0 418 3543
Size: 418 3125
BsrFI r'CCGG_y
Cuts at: 0 1384 2568 3543
Size: 1384 1184 975
Fragments arranged by size:
1384 1184 975
BsrGI T'GTAC_A
Cuts at: 0 594 1544 3543
Size: 594 950 1999
Fragments arranged by size:
1999 950 594
BstEII G'GTnAC_C
Cuts at: 0 1450 3543
Size: 1450 2093
BstXI CCAn_nnnn'nTGG
Cuts at: 0 366 736 1852 3543
Size: 366 370 1116 1691
Fragments arranged by size:
1691 1116 370 366
BstYI r'GATC_y
Cuts at: 0 1816 2824 3543
Size: 1816 1008 719
Fragments arranged by size:
1816 1008 719
BstZ17I GTA'TAC
Cuts at: 0 1758 2987 3543
Size: 1758 1229 556
Fragments arranged by size:
1758 1229 556
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2642 3543
Size: 2642 901
BtsI GCAGTG_nn'
Cuts at: 0 158 263 1199 3543
Size: 158 105 936 2344
Fragments arranged by size:
2344 936 158 105
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2346 2381 3543
Size: 2346 35 1162
Fragments arranged by size:
2346 1162 35
DraI TTT'AAA
Cuts at: 0 71 1883 2151 2648 3543
Size: 71 1812 268 497 895
Fragments arranged by size:
1812 895 497 268 71
EaeI y'GGCC_r
Cuts at: 0 1242 1386 2081 3543
Size: 1242 144 695 1462
Fragments arranged by size:
1462 1242 695 144
EarI CTCTTCn'nnn_
Cuts at: 0 1167 1569 2859 3543
Size: 1167 402 1290 684
Fragments arranged by size:
1290 1167 684 402
EcoICRI GAG'CTC
Cuts at: 0 3534 3543
Size: 3534 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 395 1230 1358 1769 1828 2025 2121
Size: 225 170 835 128 411 59 197 96
Cuts at: 2121 2325 2689 2944 3543
Size: 204 364 255 599
Fragments arranged by size:
835 599 411 364 255 225 204 197
170 128 96 59
EcoO109I rG'GnC_Cy
Cuts at: 0 613 741 1633 1859 3543
Size: 613 128 892 226 1684
Fragments arranged by size:
1684 892 613 226 128
EcoRI G'AATT_C
Cuts at: 0 1743 3543
Size: 1743 1800
EcoRV GAT'ATC
Cuts at: 0 3066 3543
Size: 3066 477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3486 3543
Size: 3486 57
HaeII r_GCGC'y
Cuts at: 0 55 3543
Size: 55 3488
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 838 870 3489 3521 3543
Size: 838 32 2619 32 22
Fragments arranged by size:
2619 838 32 32 22
HincII GTy'rAC
Cuts at: 0 255 2007 2253 2468 2778 3543
Size: 255 1752 246 215 310 765
Fragments arranged by size:
1752 765 310 255 246 215
HindIII A'AGCT_T
Cuts at: 0 1973 3543
Size: 1973 1570
HpaI GTT'AAC
Cuts at: 0 2468 3543
Size: 2468 1075
MfeI C'AATT_G
Cuts at: 0 1670 3543
Size: 1670 1873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 2433 3543
Size: 1030 1403 1110
Fragments arranged by size:
1403 1110 1030
MscI TGG'CCA
Cuts at: 0 1244 2083 3543
Size: 1244 839 1460
Fragments arranged by size:
1460 1244 839
MslI CAynn'nnrTG
Cuts at: 0 411 1448 1693 1903 3543
Size: 411 1037 245 210 1640
Fragments arranged by size:
1640 1037 411 245 210
MspA1I CmG'CkG
Cuts at: 0 30 36 2422 3289 3543
Size: 30 6 2386 867 254
Fragments arranged by size:
2386 867 254 30 6
NspI r_CATG'y
Cuts at: 0 275 392 2594 3543
Size: 275 117 2202 949
Fragments arranged by size:
2202 949 275 117
PciI A'CATG_T
Cuts at: 0 271 388 3543
Size: 271 117 3155
Fragments arranged by size:
3155 271 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 3236 3543
Size: 3236 307
PpuMI rG'GwC_Cy
Cuts at: 0 741 1859 3543
Size: 741 1118 1684
Fragments arranged by size:
1684 1118 741
PshAI GACnn'nnGTC
Cuts at: 0 455 1222 2279 3543
Size: 455 767 1057 1264
Fragments arranged by size:
1264 1057 767 455
PsiI TTA'TAA
Cuts at: 0 166 3403 3543
Size: 166 3237 140
Fragments arranged by size:
3237 166 140
PstI C_TGCA'G
Cuts at: 0 1286 2177 3151 3294 3543
Size: 1286 891 974 143 249
Fragments arranged by size:
1286 974 891 249 143
PvuII CAG'CTG
Cuts at: 0 2422 3289 3543
Size: 2422 867 254
Fragments arranged by size:
2422 867 254
SacI G_AGCT'C
Cuts at: 0 3536 3543
Size: 3536 7
ScaI AGT'ACT
Cuts at: 0 1849 3543
Size: 1849 1694
SfcI C'TryA_G
Cuts at: 0 1282 1967 2173 3147 3162 3290 3543
Size: 1282 685 206 974 15 128 253
Fragments arranged by size:
1282 974 685 253 206 128 15
SmlI C'TyrA_G
Cuts at: 0 2426 2736 3543
Size: 2426 310 807
Fragments arranged by size:
2426 807 310
SspI AAT'ATT
Cuts at: 0 116 3543
Size: 116 3427
StuI AGG'CCT
Cuts at: 0 602 3543
Size: 602 2941
StyI C'CwwG_G
Cuts at: 0 559 1862 2333 3543
Size: 559 1303 471 1210
Fragments arranged by size:
1303 1210 559 471
SwaI ATTT'AAAT
Cuts at: 0 1883 3543
Size: 1883 1660
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1177 3543
Size: 1177 2366
TatI w'GTAC_w
Cuts at: 0 594 1544 1847 3273 3543
Size: 594 950 303 1426 270
Fragments arranged by size:
1426 950 594 303 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 378 410 3543
Size: 378 32 3133
Fragments arranged by size:
3133 378 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 179 2362 3543
Size: 179 2183 1181
Fragments arranged by size:
2183 1181 179
XmnI GAAnn'nnTTC
Cuts at: 0 3156 3543
Size: 3156 387
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbsI BclI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstEII BstXI BstYI BstZ17I BtgZI
BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NspI PciI PflMI
PpuMI PshAI PsiI PstI PvuII SacI ScaI SfcI
SmlI SspI StuI StyI SwaI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AgeI AhdI ApaI AscI AseI AsiSI
AvrII BbeI BbvCI BcgI BciVI BfrBI BlpI BmtI
Bpu10I BsaAI BsaXI BsiEI BsiWI BspEI BspHI BsrBI
BssHII BssSI BstAPI BstBI Bsu36I BtgI ClaI DraIII
DrdI EagI EciI EcoNI FseI FspI FspAI KasI
KpnI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI Tth111I XbaI XhoI
XmaI ZraI