################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmI BspEI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HaeII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 1258 GTCTAC GT^[AC][GT]AC AgeI 839 ACCGGT A^CCGGT AlwI 426 GATCC GGATC(4/5) ApaI 1407 GGGCCC GGGCC^C AspI 808 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 842 GGTCC G^G[AT]CC BanI 1327 GGCACC G^G[CT][GA]CC BbvCI 1039 GCTGAGG CCTCAGC(-5/-2) BcgI 1109 GCAACAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 144 TGATCA T^GATCA BglI 1096 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1168 CTGGAG CTGGAG(16/14) BsaWI 839 ACCGGT [AT]^CCGG[AT] BsmBI 66 CGTCTC CGTCTC(1/5) Bsp1407I 1220 TGTACA T^GTACA BspDI 1087 ATCGAT AT^CGAT BsrGI 1220 TGTACA T^GTACA BstYI 425 AGATCC [GA]^GATC[CT] CfoI 1431 GCGC GCG^C ClaI 1087 ATCGAT AT^CGAT CpoI 841 CGGTCCG CG^G[AT]CCG DrdI 676 GACGGGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 429 CCTTATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 144 TGATCA T^GATCA HhaI 1431 GCGC GCG^C HinP1I 1431 GCGC G^CGC HindIII 1279 AAGCTT A^AGCTT MaeII 1256 ACGT A^CGT MflI 425 AGATCC [GA]^GATC[CT] MslI 1422 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1026 GCCGGC GCC^GGC NcoI 1454 CCATGG C^CATGG NgoMIV 1026 GCCGGC G^CCGGC PflFI 808 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 29 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 839 ACCGGT A^CCGGT PvuII 601 CAGCTG CAG^CTG RsrII 841 CGGTCCG CG^G[AT]CCG SapI 595 GAAGAGC GCTCTTC(1/4) SexAI 512 ACCAGGT A^CC[AT]GGT SmaI 59 CCCGGG CCC^GGG SspBI 1220 TGTACA T^GTACA StuI 1079 AGGCCT AGG^CCT TfiI 997 GATTC G^A[AT]TC Tth111I 808 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 29 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1157 CCATCAGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 425 AGATCC [GA]^GATC[CT] XmaCI 59 CCCGGG C^CCGGG XmaI 59 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 403 CGCG CG^CG 811 CGCG AspHI 1237 GAGCTC G[AT]GC[AT]^C 1567 GAGCTC AvaI 59 CCCGGG C^[CT]CG[GA]G 1303 CTCGGG BsaI 1450 GAGACC GGTCTC(1/5) 684 GGTCTC BsgI 228 CTGCAC GTGCAG(16/14) 1006 GTGCAG BsiEI 807 CGACCG CG[GA][CT]^CG 1132 CGGTCG BsiHKAI 1237 GAGCTC G[AT]GC[AT]^C 1567 GAGCTC BsoBI 59 CCCGGG C^[CT]CG[GA]G 1303 CTCGGG BspMI 112 ACCTGC ACCTGC(4/8) 343 ACCTGC BsrBI 1416 CCGCTC CCGCTC(-3/-3) 1129 GAGCGG BsrFI 839 ACCGGT [GA]^CCGG[CT] 1026 GCCGGC BstAPI 871 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1391 GCACGGGCTGC BstEII 396 GGTCACC G^GT[ACGT]ACC 1458 GGTGACC BstPI 396 GGTCACC G^GT[ACGT]ACC 1458 GGTGACC BstUI 403 CGCG CG^CG 811 CGCG Cfr10I 839 ACCGGT [GA]^CCGG[CT] 1026 GCCGGC DpnII 145 GATC ^GATC 426 GATC DsaI 41 CCGTGG C^C[GA][CT]GG 1454 CCATGG EcoO65I 396 GGTCACC G^GT[ACGT]ACC 1458 GGTGACC HincII 151 GTCAAC GT[CT]^[GA]AC 274 GTCAAC HindII 151 GTCAAC GT[CT]^[GA]AC 274 GTCAAC MboI 145 GATC ^GATC 426 GATC MvnI 403 CGCG CG^CG 811 CGCG NdeII 145 GATC ^GATC 426 GATC PstI 379 CTGCAG CTGCA^G 700 CTGCAG SacI 1237 GAGCTC GAGCT^C 1567 GAGCTC Sau3AI 145 GATC ^GATC 426 GATC TaqI 1088 TCGA T^CGA 1118 TCGA TthHB8I 1088 TCGA T^CGA 1118 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcyI AfaI AluI AlwNI BanII BbsI BbvI BcnI BmyI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI