Restriction map for pF1KA0386
 (Linear) (Six-base) MAPSORT of: KIAA0386.seq  Check: 2485  from: 1  to: 3204



KIAA0386.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AccI GT'mk_AC

Cuts at:      0     230     896    2477    3204 
   Size:        230     666    1581     727

  Fragments arranged by size: 

                1581     727     666     230
 
Acc65I G'GTAC_C

Cuts at:      0     913    1036    3204 
   Size:        913     123    2168

  Fragments arranged by size: 

                2168     913     123
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     991    1158    1281    1519    1799    2174    3083 
   Size:        175     816     167     123     238     280     375     909

Cuts at:   3083    3204 
   Size:        121

  Fragments arranged by size: 

                 909     816     375     280     238     175     167     123
                 121
 
AfeI AGC'GCT

Cuts at:      0    2147    3204 
   Size:       2147    1057
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3047    3204 
   Size:       3047     157
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3049    3204 
   Size:       3049     155
 
AlwNI CAG_nnn'CTG

Cuts at:      0     985    1576    1881    2083    2745    3204 
   Size:        985     591     305     202     662     459

  Fragments arranged by size: 

                 985     662     591     459     305     202
 
ApaI G_GGCC'C

Cuts at:      0      38    3204 
   Size:         38    3166
 
ApaLI G'TGCA_C

Cuts at:      0     570    3204 
   Size:        570    2634
 
ApoI r'AATT_y

Cuts at:      0     120     607     671    1899    2416    2591    3142    3204 
   Size:        120     487      64    1228     517     175     551      62

  Fragments arranged by size: 

                1228     551     517     487     175     120      64      62
            
 
AvaI C'yCGr_G

Cuts at:      0     515    3149    3204 
   Size:        515    2634      55

  Fragments arranged by size: 

                2634     515      55
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     897     930    3204 
   Size:        897      33    2274

  Fragments arranged by size: 

                2274     897      33
 
BamHI G'GATC_C

Cuts at:      0      10    3204 
   Size:         10    3194
 
BanI G'GyrC_C

Cuts at:      0     463     913    1036    1725    2240    3204 
   Size:        463     450     123     689     515     964

  Fragments arranged by size: 

                 964     689     515     463     450     123
 
BanII G_rGCy'C

Cuts at:      0      38     809    1223    1523    1652    3204 
   Size:         38     771     414     300     129    1552

  Fragments arranged by size: 

                1552     771     414     300     129      38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1514    1940    2162    3204 
   Size:       1514     426     222    1042

  Fragments arranged by size: 

                1514    1042     426     222
 
BbvCI CC'TCA_GC

Cuts at:      0     206    1402    3204 
   Size:        206    1196    1802

  Fragments arranged by size: 

                1802    1196     206
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2244    2278    3204 
   Size:       2244      34     926

  Fragments arranged by size: 

                2244     926      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     451     956    3204 
   Size:        451     505    2248

  Fragments arranged by size: 

                2248     505     451
 
BglI GCCn_nnn'nGGC

Cuts at:      0      33    1110    3204 
   Size:         33    1077    2094

  Fragments arranged by size: 

                2094    1077      33
 
BlpI GC'TnA_GC

Cuts at:      0    1291    1446    1583    2606    3204 
   Size:       1291     155     137    1023     598

  Fragments arranged by size: 

                1291    1023     598     155     137
 
Bme1580I G_kGCm'C

Cuts at:      0      38     574    3204 
   Size:         38     536    2630

  Fragments arranged by size: 

                2630     536      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       9    2560    3204 
   Size:          9    2551     644

  Fragments arranged by size: 

                2551     644       9
 
BmtI G_CTAG'C

Cuts at:      0     820    3204 
   Size:        820    2384
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1659    3204 
   Size:       1659    1545
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50      67     286     415     439     677    2230    2533 
   Size:         50      17     219     129      24     238    1553     303

Cuts at:   2533    2607    2716    2956    2962    3204 
   Size:         74     109     240       6     242

  Fragments arranged by size: 

                1553     303     242     240     238     219     129     109
                  74      50      24      17       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     206    1402    2707    3204 
   Size:        206    1196    1305     497

  Fragments arranged by size: 

                1305    1196     497     206
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    1143    1618    1636    2268    3031    3204 
   Size:        118    1025     475      18     632     763     173

  Fragments arranged by size: 

                1025     763     632     475     173     118      18
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     847    3204 
   Size:        847    2357
 
BsaBI GATnn'nnATC

Cuts at:      0     260    3204 
   Size:        260    2944
 
BsaHI Gr'CG_yC

Cuts at:      0     390     450    2684    3204 
   Size:        390      60    2234     520

  Fragments arranged by size: 

                2234     520     390      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1171    1201    3204 
   Size:       1171      30    2003

  Fragments arranged by size: 

                2003    1171      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1026    1285    1531    1651    2035    2038    3204 
   Size:       1026     259     246     120     384       3    1166

  Fragments arranged by size: 

                1166    1026     384     259     246     120       3
 
BseYI C'CCAG_C

Cuts at:      0     600     740     985    1576    2487    2797    2813    3204 
   Size:        600     140     245     591     911     310      16     391

  Fragments arranged by size: 

                 911     600     591     391     310     245     140      16
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1141    2238    2287    3204 
   Size:       1141    1097      49     917

  Fragments arranged by size: 

                1141    1097     917      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     574     809    1223    1589    1652    3204 
   Size:        574     235     414     366      63    1552

  Fragments arranged by size: 

                1552     574     414     366     235      63
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     381    3204 
   Size:        381    2823
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     574     809    1223    1523    1589    1652    3204 
   Size:         38     536     235     414     300      66      63    1552

  Fragments arranged by size: 

                1552     536     414     300     235      66      63      38
            
 
BspHI T'CATG_A

Cuts at:      0     677    1895    3204 
   Size:        677    1218    1309

  Fragments arranged by size: 

                1309    1218     677
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1190    1313    1539    1934    3204 
   Size:       1190     123     226     395    1270

  Fragments arranged by size: 

                1270    1190     395     226     123
 
BsrBI CCG'CTC

Cuts at:      0     130    1169    3204 
   Size:        130    1039    2035

  Fragments arranged by size: 

                2035    1039     130
 
BsrFI r'CCGG_y

Cuts at:      0     689    3204 
   Size:        689    2515
 
BsrGI T'GTAC_A

Cuts at:      0    1025    3204 
   Size:       1025    2179
 
BssSI C'ACGA_G

Cuts at:      0    2263    3204 
   Size:       2263     941
 
BstBI TT'CG_AA

Cuts at:      0    2574    3204 
   Size:       2574     630
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     582    3204 
   Size:        582    2622
 
BstYI r'GATC_y

Cuts at:      0      10    1789    1878    2336    3204 
   Size:         10    1779      89     458     868

  Fragments arranged by size: 

                1779     868     458      89      10
 
Bsu36I CC'TnA_GG

Cuts at:      0     143     881     978    1192    1569    3204 
   Size:        143     738      97     214     377    1635

  Fragments arranged by size: 

                1635     738     377     214     143      97
 
BtgI C'CryG_G

Cuts at:      0    2679    2732    3204 
   Size:       2679      53     472

  Fragments arranged by size: 

                2679     472      53
 
EaeI y'GGCC_r

Cuts at:      0     687    1733    2717    3204 
   Size:        687    1046     984     487

  Fragments arranged by size: 

                1046     984     687     487
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2069    3204 
   Size:       2069    1135
 
EcoICRI GAG'CTC

Cuts at:      0     807    1221    1650    3204 
   Size:        807     414     429    1554

  Fragments arranged by size: 

                1554     807     429     414
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50      67     175     286     415     439     677     991 
   Size:         50      17     108     111     129      24     238     314

Cuts at:    991    1158    1281    1519    1799    2174    2230    2533    2607 
   Size:        167     123     238     280     375      56     303      74

Cuts at:   2607    2716    2956    2962    3083    3204 
   Size:        109     240       6     121     121

  Fragments arranged by size: 

                 375     314     303     280     240     238     238     167
                 129     123     121     121     111     109     108      74
                  56      50      24      17       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1074    1191    1501    3204 
   Size:       1074     117     310    1703

  Fragments arranged by size: 

                1703    1074     310     117
 
EcoO109I rG'GnC_Cy

Cuts at:      0      34     236    3204 
   Size:         34     202    2968

  Fragments arranged by size: 

                2968     202      34
 
EcoRI G'AATT_C

Cuts at:      0     607    3204 
   Size:        607    2597
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     501    3204 
   Size:        501    2703
 
HaeII r_GCGC'y

Cuts at:      0     986    2149    3204 
   Size:        986    1163    1055

  Fragments arranged by size: 

                1163    1055     986
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     612     644    1171    1203    1429    1461    1474    1506 
   Size:        612      32     527      32     226      32      13      32

Cuts at:   1506    2823    2855    3033    3065    3204 
   Size:       1317      32     178      32     139

  Fragments arranged by size: 

                1317     612     527     226     178     139      32      32
                  32      32      32      32      13
 
HincII GTy'rAC

Cuts at:      0     296     897    2538    3204 
   Size:        296     601    1641     666

  Fragments arranged by size: 

                1641     666     601     296
 
HindIII A'AGCT_T

Cuts at:      0     431    1806    1986    2323    3204 
   Size:        431    1375     180     337     881

  Fragments arranged by size: 

                1375     881     431     337     180
 
KpnI G_GTAC'C

Cuts at:      0     917    1040    3204 
   Size:        917     123    2164

  Fragments arranged by size: 

                2164     917     123
 
MfeI C'AATT_G

Cuts at:      0     371    3133    3204 
   Size:        371    2762      71

  Fragments arranged by size: 

                2762     371      71
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1379    3147    3204 
   Size:       1379    1768      57

  Fragments arranged by size: 

                1768    1379      57
 
MscI TGG'CCA

Cuts at:      0    1735    2719    3204 
   Size:       1735     984     485

  Fragments arranged by size: 

                1735     984     485
 
MslI CAynn'nnrTG

Cuts at:      0     580     619    2138    3031    3204 
   Size:        580      39    1519     893     173

  Fragments arranged by size: 

                1519     893     580     173      39
 
MspA1I CmG'CkG

Cuts at:      0      77     696    1698    2362    2491    2511    2715    3204 
   Size:         77     619    1002     664     129      20     204     489

  Fragments arranged by size: 

                1002     664     619     489     204     129      77      20
            
 
NaeI GCC'GGC

Cuts at:      0     691    3204 
   Size:        691    2513
 
NcoI C'CATG_G

Cuts at:      0    2732    3204 
   Size:       2732     472
 
NdeI CA'TA_TG

Cuts at:      0    3126    3204 
   Size:       3126      78
 
NgoMIV G'CCGG_C

Cuts at:      0     689    3204 
   Size:        689    2515
 
NheI G'CTAG_C

Cuts at:      0     816    3204 
   Size:        816    2388
 
PasI CC'CwG_GG

Cuts at:      0    1467    3204 
   Size:       1467    1737
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2346    2595    3204 
   Size:       2346     249     609

  Fragments arranged by size: 

                2346     609     249
 
PfoI T'CCnGG_A

Cuts at:      0      83     455     654    1456    2550    3204 
   Size:         83     372     199     802    1094     654

  Fragments arranged by size: 

                1094     802     654     372     199      83
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1576    1608    3204 
   Size:       1576      32    1596

  Fragments arranged by size: 

                1596    1576      32
 
PspOMI G'GGCC_C

Cuts at:      0      34    3204 
   Size:         34    3170
 
PstI C_TGCA'G

Cuts at:      0    1722    1789    2464    3204 
   Size:       1722      67     675     740

  Fragments arranged by size: 

                1722     740     675      67
 
PvuII CAG'CTG

Cuts at:      0    1698    2362    2511    2715    3204 
   Size:       1698     664     149     204     489

  Fragments arranged by size: 

                1698     664     489     204     149
 
SacI G_AGCT'C

Cuts at:      0     809    1223    1652    3204 
   Size:        809     414     429    1552

  Fragments arranged by size: 

                1552     809     429     414
 
SalI G'TCGA_C

Cuts at:      0     895    3204 
   Size:        895    2309
 
ScaI AGT'ACT

Cuts at:      0    2251    2825    3204 
   Size:       2251     574     379

  Fragments arranged by size: 

                2251     574     379
 
SexAI A'CCwGG_T

Cuts at:      0     940    3204 
   Size:        940    2264
 
SfcI C'TryA_G

Cuts at:      0     231    1718    1785    2460    3204 
   Size:        231    1487      67     675     744

  Fragments arranged by size: 

                1487     744     675     231      67
 
SmaI CCC'GGG

Cuts at:      0     517    3204 
   Size:        517    2687
 
SmlI C'TyrA_G

Cuts at:      0     133    1122    1597    1651    2283    3010    3149    3204 
   Size:        133     989     475      54     632     727     139      55

  Fragments arranged by size: 

                 989     727     632     475     139     133      55      54
            
 
StuI AGG'CCT

Cuts at:      0    1642    2932    3204 
   Size:       1642    1290     272

  Fragments arranged by size: 

                1642    1290     272
 
StyI C'CwwG_G

Cuts at:      0     909    1205    2732    3204 
   Size:        909     296    1527     472

  Fragments arranged by size: 

                1527     909     472     296
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     325    2132    2272    3204 
   Size:        325    1807     140     932

  Fragments arranged by size: 

                1807     932     325     140
 
TatI w'GTAC_w

Cuts at:      0     339     554    1025    2249    2823    3204 
   Size:        339     215     471    1224     574     381

  Fragments arranged by size: 

                1224     574     471     381     339     215
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     930     962    3204 
   Size:        930      32    2242

  Fragments arranged by size: 

                2242     930      32
 
XbaI T'CTAG_A

Cuts at:      0    1797    3204 
   Size:       1797    1407
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1464    2805    3204 
   Size:       1464    1341     399

  Fragments arranged by size: 

                1464    1341     399
 
XhoI C'TCGA_G

Cuts at:      0    3149    3204 
   Size:       3149      55
 
XmaI C'CCGG_G

Cuts at:      0     515    3204 
   Size:        515    2689
 
XmnI GAAnn'nnTTC

Cuts at:      0    1619    3204 
   Size:       1619    1585
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI     AhdI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BglI     BlpI Bme1580I     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrFI    BsrGI    BssSI    BstBI    BstXI    BstYI   Bsu36I
     BtgI     EaeI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII  HindIII     KpnI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     PasI    PflMI     PfoI     PpiI   PspOMI     PstI    PvuII     SacI
     SalI     ScaI    SexAI     SfcI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII   AflIII     AgeI     AleI     AloI
     AscI     AseI    AsiSI    AvrII     BbeI     BclI    BfrBI    BglII
    BmgBI    BsaAI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BsrDI
   BssHII   BstAPI   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI    EcoRV     FseI     FspI
    FspAI     HpaI     KasI     MluI     NarI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PmlI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI  Tth111I     ZraI
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