Restriction map for pF1KSDA0053
 (Linear) (Six-base) MAPSORT of: KIAA0053.seq  Check: 5159  from: 1  to: 1914



KIAA0053.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157    1149    1154    1914 
   Size:        157     992       5     760

  Fragments arranged by size: 

                 992     760     157       5
 
AfeI AGC'GCT

Cuts at:      0     457    1822    1914 
   Size:        457    1365      92

  Fragments arranged by size: 

                1365     457      92
 
AleI CACnn'nnGTG

Cuts at:      0     658    1914 
   Size:        658    1256
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      92    1914 
   Size:         92    1822
 
ApaLI G'TGCA_C

Cuts at:      0     661    1529    1914 
   Size:        661     868     385

  Fragments arranged by size: 

                 868     661     385
 
ApoI r'AATT_y

Cuts at:      0     409     487    1145    1381    1876    1914 
   Size:        409      78     658     236     495      38

  Fragments arranged by size: 

                 658     495     409     236      78      38
 
AvaI C'yCGr_G

Cuts at:      0    1826    1914 
   Size:       1826      88
 
BanI G'GyrC_C

Cuts at:      0     504    1345    1914 
   Size:        504     841     569

  Fragments arranged by size: 

                 841     569     504
 
BanII G_rGCy'C

Cuts at:      0     713    1914 
   Size:        713    1201
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     962    1142    1914 
   Size:        962     180     772

  Fragments arranged by size: 

                 962     772     180
 
BbvCI CC'TCA_GC

Cuts at:      0     187    1914 
   Size:        187    1727
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     943     977    1914 
   Size:        943      34     937

  Fragments arranged by size: 

                 943     937      34
 
BclI T'GATC_A

Cuts at:      0     968    1013    1914 
   Size:        968      45     901

  Fragments arranged by size: 

                 968     901      45
 
BglII A'GATC_T

Cuts at:      0    1367    1914 
   Size:       1367     547
 
BlpI GC'TnA_GC

Cuts at:      0     785    1914 
   Size:        785    1129
 
Bme1580I G_kGCm'C

Cuts at:      0     509     665    1424    1533    1914 
   Size:        509     156     759     109     381

  Fragments arranged by size: 

                 759     509     381     156     109
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1601    1914 
   Size:       1601     313
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     654     998    1914 
   Size:        654     344     916

  Fragments arranged by size: 

                 916     654     344
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     562     744    1082    1634    1786    1914 
   Size:        130     432     182     338     552     152     128

  Fragments arranged by size: 

                 552     432     338     182     152     130     128
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     841    1406    1914 
   Size:        187     654     565     508

  Fragments arranged by size: 

                 654     565     508     187
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1424    1732    1914 
   Size:       1424     308     182

  Fragments arranged by size: 

                1424     308     182
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     692    1013    1914 
   Size:        692     321     901

  Fragments arranged by size: 

                 901     692     321
 
BsaWI w'CCGG_w

Cuts at:      0    1204    1230    1313    1914 
   Size:       1204      26      83     601

  Fragments arranged by size: 

                1204     601      83      26
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     974    1914 
   Size:        412     562     940

  Fragments arranged by size: 

                 940     562     412
 
BseYI C'CCAG_C

Cuts at:      0     298     625    1121    1914 
   Size:        298     327     496     793

  Fragments arranged by size: 

                 793     496     327     298
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548    1914 
   Size:        548    1366
 
BsiEI CG_ry'CG

Cuts at:      0     552    1914 
   Size:        552    1362
 
BsiHKAI G_wGCw'C

Cuts at:      0     548     665    1533    1914 
   Size:        548     117     868     381

  Fragments arranged by size: 

                 868     548     381     117
 
BsmI GAATG_Cn'

Cuts at:      0     771    1914 
   Size:        771    1143
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     605    1914 
   Size:        605    1309
 
Bsp1286I G_dGCh'C

Cuts at:      0     509     548     665     713    1424    1533    1914 
   Size:        509      39     117      48     711     109     381

  Fragments arranged by size: 

                 711     509     381     117     109      48      39
 
BspEI T'CCGG_A

Cuts at:      0    1230    1914 
   Size:       1230     684
 
BspHI T'CATG_A

Cuts at:      0     971    1914 
   Size:        971     943
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     845    1914 
   Size:        845    1069
 
BsrBI CCG'CTC

Cuts at:      0     633    1914 
   Size:        633    1281
 
BsrGI T'GTAC_A

Cuts at:      0     262    1914 
   Size:        262    1652
 
BssSI C'ACGA_G

Cuts at:      0     821    1914 
   Size:        821    1093
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     108     126     332     516    1271    1914 
   Size:        108      18     206     184     755     643

  Fragments arranged by size: 

                 755     643     206     184     108      18
 
BstYI r'GATC_y

Cuts at:      0     989    1367    1914 
   Size:        989     378     547

  Fragments arranged by size: 

                 989     547     378
 
Bsu36I CC'TnA_GG

Cuts at:      0     415    1914 
   Size:        415    1499
 
BtgI C'CryG_G

Cuts at:      0     713    1914 
   Size:        713    1201
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1235    1914 
   Size:       1235     679
 
DraI TTT'AAA

Cuts at:      0    1374    1914 
   Size:       1374     540
 
DraIII CAC_nnn'GTG

Cuts at:      0     439     669    1914 
   Size:        439     230    1245

  Fragments arranged by size: 

                1245     439     230
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     364    1027    1914 
   Size:        364     663     887

  Fragments arranged by size: 

                 887     663     364
 
EaeI y'GGCC_r

Cuts at:      0     374     450     549    1914 
   Size:        374      76      99    1365

  Fragments arranged by size: 

                1365     374      99      76
 
EagI C'GGCC_G

Cuts at:      0     549    1914 
   Size:        549    1365
 
EarI CTCTTCn'nnn_

Cuts at:      0     215     554    1039    1542    1613    1837    1914 
   Size:        215     339     485     503      71     224      77

  Fragments arranged by size: 

                 503     485     339     224     215      77      71
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     403    1914 
   Size:        403    1511
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     157     562     744    1082    1149    1154    1634 
   Size:        130      27     405     182     338      67       5     480

Cuts at:   1634    1786    1914 
   Size:        152     128

  Fragments arranged by size: 

                 480     405     338     182     152     130     128      67
                  27       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1714    1914 
   Size:       1714     200
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1508    1914 
   Size:       1508     406
 
EcoRI G'AATT_C

Cuts at:      0    1381    1914 
   Size:       1381     533
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     327    1914 
   Size:        327    1587
 
HaeII r_GCGC'y

Cuts at:      0     459    1824    1914 
   Size:        459    1365      90

  Fragments arranged by size: 

                1365     459      90
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     251     283    1914 
   Size:        251      32    1631

  Fragments arranged by size: 

                1631     251      32
 
HincII GTy'rAC

Cuts at:      0     891    1914 
   Size:        891    1023
 
HpaI GTT'AAC

Cuts at:      0     891    1914 
   Size:        891    1023
 
MscI TGG'CCA

Cuts at:      0     376     452    1914 
   Size:        376      76    1462

  Fragments arranged by size: 

                1462     376      76
 
MslI CAynn'nnrTG

Cuts at:      0     124     514     658    1914 
   Size:        124     390     144    1256

  Fragments arranged by size: 

                1256     390     144     124
 
MspA1I CmG'CkG

Cuts at:      0     108     606     876    1914 
   Size:        108     498     270    1038

  Fragments arranged by size: 

                1038     498     270     108
 
NspI r_CATG'y

Cuts at:      0     249    1914 
   Size:        249    1665
 
PasI CC'CwG_GG

Cuts at:      0     237    1253    1513    1914 
   Size:        237    1016     260     401

  Fragments arranged by size: 

                1016     401     260     237
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     459    1914 
   Size:        459    1455
 
PfoI T'CCnGG_A

Cuts at:      0     539    1914 
   Size:        539    1375
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1422    1454    1914 
   Size:       1422      32     460

  Fragments arranged by size: 

                1422     460      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1508    1914 
   Size:       1508     406
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     724     756    1914 
   Size:        724      32    1158

  Fragments arranged by size: 

                1158     724      32
 
PstI C_TGCA'G

Cuts at:      0     856    1791    1914 
   Size:        856     935     123

  Fragments arranged by size: 

                 935     856     123
 
PvuII CAG'CTG

Cuts at:      0     606     876    1914 
   Size:        606     270    1038

  Fragments arranged by size: 

                1038     606     270
 
SanDI GG'GwC_CC

Cuts at:      0    1508    1914 
   Size:       1508     406
 
SexAI A'CCwGG_T

Cuts at:      0     593    1914 
   Size:        593    1321
 
SfcI C'TryA_G

Cuts at:      0      35     831     852    1115    1787    1914 
   Size:         35     796      21     263     672     127

  Fragments arranged by size: 

                 796     672     263     127      35      21
 
SmaI CCC'GGG

Cuts at:      0    1828    1914 
   Size:       1828      86
 
SmlI C'TyrA_G

Cuts at:      0    1439    1711    1914 
   Size:       1439     272     203

  Fragments arranged by size: 

                1439     272     203
 
StuI AGG'CCT

Cuts at:      0    1855    1914 
   Size:       1855      59
 
StyI C'CwwG_G

Cuts at:      0    1046    1556    1856    1914 
   Size:       1046     510     300      58

  Fragments arranged by size: 

                1046     510     300      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      32    1914 
   Size:         32    1882
 
TatI w'GTAC_w

Cuts at:      0     262    1914 
   Size:        262    1652
 
XmaI C'CCGG_G

Cuts at:      0    1826    1914 
   Size:       1826      88
 
XmnI GAAnn'nnTTC

Cuts at:      0     320    1321    1914 
   Size:        320    1001     593

  Fragments arranged by size: 

                1001     593     320
 

 Enzymes that do cut:

     AcuI     AfeI     AleI     AlfI    ApaLI     ApoI     AvaI     BanI
    BanII     BbsI    BbvCI     BcgI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrGI    BssSI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII     HpaI     MscI     MslI   MspA1I     NspI     PasI    PflMI
     PfoI     PpiI    PpuMI     PsrI     PstI    PvuII    SanDI    SexAI
     SfcI     SmaI     SmlI     StuI     StyI    TaqII     TatI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BciVI    BfrBI     BglI    BmgBI     BmtI
    BsaAI    BsaBI    BsaHI    BsaXI    BsiWI    BsrDI    BsrFI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I     BtsI     ClaI    CspCI  EcoICRI
    EcoRV     FseI     FspI    FspAI  HindIII     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI
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