Restriction map for pF1KA1204
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AhdI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbrPI       BciVI       BclI        BfrI        
BlnI        BsaAI       BsaBI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstZ17I     ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        ScaI        SexAI       SfiI        
SnaBI       SpeI        SphI        SspBI       Tth111I     


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AgeI         1108  ACCGGT               A^CCGGT
Alw44I        656  GTGCAC               G^TGCAC
ApaLI         656  GTGCAC               G^TGCAC
BglI         2248  GCCAGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           16  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I       16  GCTAAGC              GC^T[ACGT]AGC
BsmBI        2610  CGTCTC               CGTCTC(1/5)
BspHI         695  TCATGA               T^CATGA
BstBI        1368  TTCGAA               TT^CGAA
BstEII       2806  GGTCACC              G^GT[ACGT]ACC
BstPI        2806  GGTCACC              G^GT[ACGT]ACC
CelII          16  GCTAAGC              GC^T[ACGT]AGC
DraI         1794  TTTAAA               TTT^AAA
DrdI         3415  GACCCAAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      2806  GGTCACC              G^GT[ACGT]ACC
EcoRI        2946  GAATTC               G^AATTC
HgaI         2773  GACGC                GACGC(5/10)
HincII       2379  GTCAAC               GT[CT]^[GA]AC
HindII       2379  GTCAAC               GT[CT]^[GA]AC
NcoI          836  CCATGG               C^CATGG
NspV         1368  TTCGAA               TT^CGAA
PinAI        1108  ACCGGT               A^CCGGT
RcaI          695  TCATGA               T^CATGA
SbfI         1875  CCTGCAGG             CCTGCA^GG
SfuI         1368  TTCGAA               TT^CGAA
SgrAI        1107  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         1213  CCCGGG               CCC^GGG
Sse8387I     1875  CCTGCAGG             CCTGCA^GG
SspI          918  AATATT               AAT^ATT
SwaI         1793  ATTTAAAT             ATTT^AAAT
XbaI         2862  TCTAGA               T^CTAGA
XcmI         3418  CCAAAGGTCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1213  CCCGGG               C^CCGGG
XmaI         1213  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII       1320  TCCGGA               T^CCGGA
             1821  TCCGGA              
AcyI          522  GGCGCC               G[GA]^CG[CT]C
             1741  GGCGCC              
AocI          534  CCTCAGG              CC^T[ACGT]AGG
             3009  CCTGAGG             
ApaI         2071  GGGCCC               GGGCC^C
             3286  GGGCCC              
Asp700I      1296  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2266  GAAATAGTTC          
BamHI        1317  GGATCC               G^GATCC
             2160  GGATCC              
BbeI          522  GGCGCC               GGCGC^C
             1741  GGCGCC              
BbvCI        4282  CCTCAGC              CCTCAGC(-5/-2)
             1219  GCTGAGG             
BcgI         1461  CGAGCCCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1538  CGAAGGAGCTGC        
BsaHI         522  GGCGCC               G[GA]^CG[CT]C
             1741  GGCGCC              
BseAI        1320  TCCGGA               T^CCGGA
             1821  TCCGGA              
BspEI        1320  TCCGGA               T^CCGGA
             1821  TCCGGA              
BsrBI        1483  CCGCTC               CCGCTC(-3/-3)
             1593  GAGCGG              
BstAPI       1517  GCACGCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2226  GCAGGGCCTGC         
BstXI         731  CCATGAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2135  CCACCCCTCTGG        
Bsu36I        534  CCTCAGG              CC^T[ACGT]AGG
             3009  CCTGAGG             
DsaI          836  CCATGG               C^C[GA][CT]GG
             2876  CCACGG              
Eco81I        534  CCTCAGG              CC^T[ACGT]AGG
             3009  CCTGAGG             
FauI          764  CCCGC                CCCGC(4/6)
             3253  CCCGC               
Hin1I         522  GGCGCC               G[GA]^CG[CT]C
             1741  GGCGCC              
KasI          522  GGCGCC               G^GCGCC
             1741  GGCGCC              
MaeII         107  ACGT                 A^CGT
              933  ACGT                
MroI         1320  TCCGGA               T^CCGGA
             1821  TCCGGA              
MslI         1792  CATTTAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3426  CACATGGATG          
NarI          522  GGCGCC               GG^CGCC
             1741  GGCGCC              
PaeR7I       2056  CTCGAG               C^TCGAG
             2899  CTCGAG              
SapI          114  GAAGAGC              GCTCTTC(1/4)
              699  GAAGAGC             
SfoI          522  GGCGCC               GGC^GCC
             1741  GGCGCC              
StuI         1722  AGGCCT               AGG^CCT
             3307  AGGCCT              
XhoI         2056  CTCGAG               C^TCGAG
             2899  CTCGAG              
XmnI         1296  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2266  GAAATAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       
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