################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BciVI BclI BfrI BlnI BsaBI BsiWI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KpnI MamI MluI MseI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 1931 TCCGGA T^CCGGA AcsI 1147 AAATTT [GA]^AATT[CT] AflIII 1383 ACACGT A^C[GA][CT]GT Aor51HI 191 AGCGCT AGC^GCT ApaI 1747 GGGCCC GGGCC^C ApoI 1147 AAATTT [GA]^AATT[CT] BamHI 1040 GGATCC G^GATCC BbrPI 1384 CACGTG CAC^GTG BglI 1648 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2316 AGATCT A^GATCT BlpI 1870 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1870 GCTCAGC GC^T[ACGT]AGC BsaAI 1384 CACGTG [CT]AC^GT[GA] BsaI 2202 GAGACC GGTCTC(1/5) BsaWI 1931 TCCGGA [AT]^CCGG[AT] BseAI 1931 TCCGGA T^CCGGA BsmI 1092 GAATGC GAATGC(1/-1) Bsp1407I 602 TGTACA T^GTACA BspEI 1931 TCCGGA T^CCGGA BsrGI 602 TGTACA T^GTACA CelII 1870 GCTCAGC GC^T[ACGT]AGC Eco47III 191 AGCGCT AGC^GCT EcoNI 84 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 2361 GTCGAC GT[CT]^[GA]AC HindII 2361 GTCGAC GT[CT]^[GA]AC HindIII 1075 AAGCTT A^AGCTT KspI 1703 CCGCGG CCGC^GG MroI 1931 TCCGGA T^CCGGA MslI 1542 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1466 GCCGGC GCC^GGC NgoMIV 1466 GCCGGC G^CCGGC PmaCI 1384 CACGTG CAC^GTG PmlI 1384 CACGTG CAC^GTG PshAI 1912 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 1703 CCGCGG CCGC^GG SalI 2361 GTCGAC G^TCGAC ScaI 944 AGTACT AGT^ACT SexAI 460 ACCAGGT A^CC[AT]GGT SmlI 838 CTTGAG C^T[CT][GA]AG SspBI 602 TGTACA T^GTACA SspI 400 AATATT AAT^ATT StuI 734 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 2310 GTCTAC GT^[AC][GT]AC 2361 GTCGAC AcyI 1445 GGCGCC G[GA]^CG[CT]C 1879 GGCGCC Asp700I 6 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 79 GAAGACCTTC BbeI 1445 GGCGCC GGCGC^C 1879 GGCGCC BbsI 79 GAAGAC GAAGAC(2/6) 483 GTCTTC BbvCI 1473 CCTCAGC CCTCAGC(-5/-2) 2221 CCTCAGC BcgI 1650 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 9 GCAGTTCAATCG BfaI 2252 CTAG C^TAG 2281 CTAG BpuAI 79 GAAGAC GAAGAC(2/6) 483 GTCTTC BsaHI 1445 GGCGCC G[GA]^CG[CT]C 1879 GGCGCC BsmBI 298 GAGACG CGTCTC(1/5) 353 GAGACG BstYI 1040 GGATCC [GA]^GATC[CT] 2316 AGATCT DrdI 474 GACTTCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1269 GACCACAGTGTC EagI 792 CGGCCG C^GGCCG 1592 CGGCCG EclXI 792 CGGCCG C^GGCCG 1592 CGGCCG Eco52I 792 CGGCCG C^GGCCG 1592 CGGCCG Hin1I 1445 GGCGCC G[GA]^CG[CT]C 1879 GGCGCC KasI 1445 GGCGCC G^GCGCC 1879 GGCGCC MaeI 2252 CTAG C^TAG 2281 CTAG MaeII 356 ACGT A^CGT 1385 ACGT MfeI 965 CAATTG C^AATTG 1208 CAATTG MflI 1040 GGATCC [GA]^GATC[CT] 2316 AGATCT MunI 965 CAATTG C^AATTG 1208 CAATTG NarI 1445 GGCGCC GG^CGCC 1879 GGCGCC PflMI 39 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1536 CCAAAGCATGG PpuMI 1218 AGGACCC [GA]G^G[AT]CC[CT] 1970 AGGTCCC SacI 1868 GAGCTC GAGCT^C 2353 GAGCTC SfoI 1445 GGCGCC GGC^GCC 1879 GGCGCC SphI 20 GCATGC GCATG^C 1317 GCATGC TfiI 448 GATTC G^A[AT]TC 1959 GATTC Tsp45I 826 GTGAC ^GT[GC]AC 2061 GTCAC Van91I 39 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1536 CCAAAGCATGG XcmI 35 CCAACCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 428 CCAAGTTGGTGCTGG XhoII 1040 GGATCC [GA]^GATC[CT] 2316 AGATCT XmnI 6 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 79 GAAGACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp509I TspRI TthHB8I