(Circular) (Six-base) MAPSORT of: pF1KA0672.seq Check: 4819 from: 1 to: 5567 pF1KA0672 5567 bp With 182 enzymes: SgfI * June 20, 2008 10:19 .. AccI GT'mk_AC Cuts at: 2419 2470 2605 2419 Size: 51 135 5381 Fragments arranged by size: 5381 135 51 Acc65I G'GTAC_C Cuts at: 2583 2583 Size: 5567 AclI AA'CG_TT Cuts at: 5407 5407 Size: 5567 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 181 578 1024 1171 3392 3824 4571 4839 181 Size: 397 446 147 2221 432 747 268 909 Fragments arranged by size: 2221 909 747 446 432 397 268 147 AfeI AGC'GCT Cuts at: 301 301 Size: 5567 AflIII A'CryG_T Cuts at: 1491 2919 4023 5000 1491 Size: 1428 1104 977 2058 Fragments arranged by size: 2058 1428 1104 977 AgeI A'CCGG_T Cuts at: 3955 3955 Size: 5567 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1403 1435 1441 1473 1403 Size: 32 6 32 5497 Fragments arranged by size: 5497 32 32 6 AlwNI CAG_nnn'CTG Cuts at: 292 339 432 1800 1903 2518 2872 4439 292 Size: 47 93 1368 103 615 354 1567 1420 Fragments arranged by size: 1567 1420 1368 615 354 103 93 47 ApaI G_GGCC'C Cuts at: 1859 1859 Size: 5567 ApaLI G'TGCA_C Cuts at: 4337 4337 Size: 5567 ApoI r'AATT_y Cuts at: 1255 2571 4920 5176 1255 Size: 1316 2349 256 1646 Fragments arranged by size: 2349 1646 1316 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5567 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5567 AvaI C'yCGr_G Cuts at: 1561 1858 2211 2464 2587 1561 Size: 297 353 253 123 4541 Fragments arranged by size: 4541 353 297 253 123 BamHI G'GATC_C Cuts at: 1148 2592 1148 Size: 1444 4123 BanI G'GyrC_C Cuts at: 625 1553 1987 2061 2583 2997 3245 3280 625 Size: 928 434 74 522 414 248 35 2912 Fragments arranged by size: 2912 928 522 434 414 248 74 35 BanII G_rGCy'C Cuts at: 476 1859 1968 1980 2465 2581 3611 476 Size: 1383 109 12 485 116 1030 2432 Fragments arranged by size: 2432 1383 1030 485 116 109 12 BbeI G_GCGC'C Cuts at: 1557 1991 3001 3249 1557 Size: 434 1010 248 3875 Fragments arranged by size: 3875 1010 434 248 BbsI GAAGACnn'nnnn_ Cuts at: 194 584 194 Size: 390 5177 BbvCI CC'TCA_GC Cuts at: 1582 2330 1582 Size: 748 4819 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 106 140 1747 1781 2541 2575 106 Size: 34 1607 34 760 34 3098 Fragments arranged by size: 3098 1607 760 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3465 4226 5567 3465 Size: 761 1341 3465 Fragments arranged by size: 3465 1341 761 BclI T'GATC_A Cuts at: 3087 3087 Size: 5567 BglI GCCn_nnn'nGGC Cuts at: 1762 1762 Size: 5567 BglII A'GATC_T Cuts at: 2424 3082 5119 2424 Size: 658 2037 2872 Fragments arranged by size: 2872 2037 658 BlpI GC'TnA_GC Cuts at: 1979 2679 1979 Size: 700 4867 Bme1580I G_kGCm'C Cuts at: 1859 1939 3192 3285 4341 1859 Size: 80 1253 93 1056 3085 Fragments arranged by size: 3085 1253 1056 93 80 BmgBI CAC'GTC Cuts at: 465 465 Size: 5567 BmrI ACTGGGnnnn_n' Cuts at: 1298 1686 2128 2779 2860 3195 5339 1298 Size: 388 442 651 81 335 2144 1526 Fragments arranged by size: 2144 1526 651 442 388 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2207 2333 3934 2207 Size: 126 1601 3840 Fragments arranged by size: 3840 1601 126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 235 898 1630 1831 1880 1911 1994 2512 235 Size: 663 732 201 49 31 83 518 3290 Fragments arranged by size: 3290 732 663 518 201 83 49 31 Bpu10I CC'TnA_GC Cuts at: 425 1582 2297 2330 425 Size: 1157 715 33 3662 Fragments arranged by size: 3662 1157 715 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 967 2743 3622 4114 4412 967 Size: 1776 879 492 298 2122 Fragments arranged by size: 2122 1776 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 2304 5271 2304 Size: 2967 2600 BsaAI yAC'GTr Cuts at: 1494 2920 1494 Size: 1426 4141 BsaBI GATnn'nnATC Cuts at: 6 3081 5118 6 Size: 3075 2037 455 Fragments arranged by size: 3075 2037 455 BsaHI Gr'CG_yC Cuts at: 1554 1988 2998 3246 3948 5444 1554 Size: 434 1010 248 702 1496 1677 Fragments arranged by size: 1677 1496 1010 702 434 248 BsaWI w'CCGG_w Cuts at: 2039 2756 3277 3955 4229 4376 4787 2039 Size: 717 521 678 274 147 411 2819 Fragments arranged by size: 2819 717 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 286 316 1319 1349 1441 1471 2527 2557 286 Size: 30 1003 30 92 30 1056 30 3296 Fragments arranged by size: 3296 1056 1003 92 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 895 979 1686 2228 2318 2551 2554 895 Size: 84 707 542 90 233 3 3908 Fragments arranged by size: 3908 707 542 233 90 84 3 BseYI C'CCAG_C Cuts at: 1996 2002 2158 2226 2992 3750 4327 4823 1996 Size: 6 156 68 766 758 577 496 2740 Fragments arranged by size: 2740 766 758 577 496 156 68 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1066 1889 1967 1066 Size: 823 78 4666 Fragments arranged by size: 4666 823 78 BsiEI CG_ry'CG Cuts at: 104 903 1703 1707 3109 3155 4363 104 Size: 799 800 4 1402 46 1208 1308 Fragments arranged by size: 1402 1308 1208 800 799 46 4 BsiHKAI G_wGCw'C Cuts at: 887 1980 2465 2498 2554 2581 3359 3549 4341 Size: 1093 485 33 56 27 778 190 792 Cuts at: 4341 887 Size: 2113 Fragments arranged by size: 2113 1093 792 778 485 190 56 33 27 BsmI GAATG_Cn' Cuts at: 1206 1206 Size: 5567 BsmBI CGTCTCn'nnnn_ Cuts at: 400 455 400 Size: 55 5512 Bsp1286I G_dGCh'C Cuts at: 476 887 1859 1939 1968 1980 2465 2498 2554 Size: 411 972 80 29 12 485 33 56 Cuts at: 2554 2581 3192 3285 3359 3549 3611 4341 476 Size: 27 611 93 74 190 62 730 1702 Fragments arranged by size: 1702 972 730 611 485 411 190 93 80 74 62 56 33 29 27 12 BspEI T'CCGG_A Cuts at: 2039 4787 2039 Size: 2748 2819 BspHI T'CATG_A Cuts at: 4743 4743 Size: 5567 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2617 2831 3133 3514 2617 Size: 214 302 381 4670 Fragments arranged by size: 4670 381 302 214 BsrBI CCG'CTC Cuts at: 50 3859 3913 5397 5562 50 Size: 3809 54 1484 165 55 Fragments arranged by size: 3809 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3479 3479 Size: 5567 BsrFI r'CCGG_y Cuts at: 1550 1574 1702 3565 3746 3955 1550 Size: 24 128 1863 181 209 3162 Fragments arranged by size: 3162 1863 209 181 128 24 BsrGI T'GTAC_A Cuts at: 710 710 Size: 5567 BssSI C'ACGA_G Cuts at: 3838 4196 3838 Size: 358 5209 BstAPI GCAn_nnn'nTGC Cuts at: 2944 2944 Size: 5567 BstBI TT'CG_AA Cuts at: 3928 3928 Size: 5567 BstXI CCAn_nnnn'nTGG Cuts at: 675 1311 2119 675 Size: 636 808 4123 Fragments arranged by size: 4123 808 636 BstYI r'GATC_y Cuts at: 1 1148 2424 2592 3059 3082 3417 3663 4675 Size: 1147 1276 168 467 23 335 246 1012 Cuts at: 4675 4761 4773 5119 1 Size: 86 12 346 449 Fragments arranged by size: 1276 1147 1012 467 449 346 335 246 168 86 23 12 Bsu36I CC'TnA_GG Cuts at: 643 1633 2192 643 Size: 990 559 4018 Fragments arranged by size: 4018 990 559 BtgI C'CryG_G Cuts at: 1592 1811 2372 1592 Size: 219 561 4787 Fragments arranged by size: 4787 561 219 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3517 3698 3861 3517 Size: 181 163 5223 Fragments arranged by size: 5223 181 163 BtsI GCAGTG_nn' Cuts at: 2810 2810 Size: 5567 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1661 1696 1661 Size: 35 5532 DraI TTT'AAA Cuts at: 2566 2566 Size: 5567 DrdI GACnn_nn'nnGTC Cuts at: 588 1383 3273 4131 588 Size: 795 1890 858 2024 Fragments arranged by size: 2024 1890 858 795 EaeI y'GGCC_r Cuts at: 140 722 900 1342 1700 2088 2121 3152 3326 Size: 582 178 442 358 388 33 1031 174 Cuts at: 3326 3717 3744 5089 140 Size: 391 27 1345 618 Fragments arranged by size: 1345 1031 618 582 442 391 388 358 178 174 33 27 EagI C'GGCC_G Cuts at: 900 1700 3152 900 Size: 800 1452 3315 Fragments arranged by size: 3315 1452 800 EarI CTCTTCn'nnn_ Cuts at: 653 979 1683 3590 3800 653 Size: 326 704 1907 210 2420 Fragments arranged by size: 2420 1907 704 326 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 505 2467 3775 4085 4231 5377 505 Size: 1962 1308 310 146 1146 695 Fragments arranged by size: 1962 1308 1146 695 310 146 EcoICRI GAG'CTC Cuts at: 1978 2463 2579 1978 Size: 485 116 4966 Fragments arranged by size: 4966 485 116 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 181 235 578 898 1024 1171 1630 1831 1880 Size: 54 343 320 126 147 459 201 49 Cuts at: 1880 1911 1994 2512 3392 3824 4571 4839 181 Size: 31 83 518 880 432 747 268 909 Fragments arranged by size: 909 880 747 518 459 432 343 320 268 201 147 126 83 54 49 31 EcoNI CCTnn'n_nnAGG Cuts at: 196 196 Size: 5567 EcoO109I rG'GnC_Cy Cuts at: 1327 2079 2379 2706 1327 Size: 752 300 327 4188 Fragments arranged by size: 4188 752 327 300 EcoRI G'AATT_C Cuts at: 2571 2571 Size: 5567 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 211 879 2100 3586 211 Size: 668 1221 1486 2192 Fragments arranged by size: 2192 1486 1221 668 FspI TGC'GCA Cuts at: 3348 3348 Size: 5567 HaeII r_GCGC'y Cuts at: 303 1557 1991 3001 3249 4271 5250 303 Size: 1254 434 1010 248 1022 979 620 Fragments arranged by size: 1254 1022 1010 979 620 434 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 286 318 682 714 1319 1351 2383 2415 2431 Size: 32 364 32 605 32 1032 32 16 Cuts at: 2431 2452 2463 2484 2527 2559 3409 3439 3441 Size: 21 11 21 43 32 850 30 2 Cuts at: 3441 3471 286 Size: 30 2382 Fragments arranged by size: 2382 1032 850 605 364 43 32 32 32 32 32 30 30 21 21 16 11 2 HincII GTy'rAC Cuts at: 2471 2606 2471 Size: 135 5432 HindIII A'AGCT_T Cuts at: 1183 1183 Size: 5567 KasI G'GCGC_C Cuts at: 1553 1987 2997 3245 1553 Size: 434 1010 248 3875 Fragments arranged by size: 3875 1010 434 248 KpnI G_GTAC'C Cuts at: 2587 2587 Size: 5567 MfeI C'AATT_G Cuts at: 1073 1316 1073 Size: 243 5324 MluI A'CGCG_T Cuts at: 5000 5000 Size: 5567 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1687 4238 4422 1687 Size: 2551 184 2832 Fragments arranged by size: 2832 2551 184 MscI TGG'CCA Cuts at: 142 724 1344 2090 2123 3328 142 Size: 582 620 746 33 1205 2381 Fragments arranged by size: 2381 1205 746 620 582 33 MslI CAynn'nnrTG Cuts at: 1654 3556 3683 1654 Size: 1902 127 3538 Fragments arranged by size: 3538 1902 127 MspA1I CmG'CkG Cuts at: 138 703 876 899 1785 1813 2119 2127 2230 Size: 565 173 23 886 28 306 8 103 Cuts at: 2230 2678 2992 3352 4365 4610 138 Size: 448 314 360 1013 245 1095 Fragments arranged by size: 1095 1013 886 565 448 360 314 306 245 173 103 28 23 8 NaeI GCC'GGC Cuts at: 1576 3748 1576 Size: 2172 3395 NarI GG'CG_CC Cuts at: 1554 1988 2998 3246 1554 Size: 434 1010 248 3875 Fragments arranged by size: 3875 1010 434 248 NgoMIV G'CCGG_C Cuts at: 1574 3746 1574 Size: 2172 3395 NspI r_CATG'y Cuts at: 132 266 1429 2400 2418 2502 2620 3120 4027 Size: 134 1163 971 18 84 118 500 907 Cuts at: 4027 132 Size: 1672 Fragments arranged by size: 1672 1163 971 907 500 134 118 84 18 PasI CC'CwG_GG Cuts at: 1444 2404 1444 Size: 960 4607 PciI A'CATG_T Cuts at: 4023 4023 Size: 5567 PflMI CCAn_nnn'nTGG Cuts at: 153 1650 153 Size: 1497 4070 PfoI T'CCnGG_A Cuts at: 1166 1166 Size: 5567 PmeI GTTT'AAAC Cuts at: 2566 2566 Size: 5567 PmlI CAC'GTG Cuts at: 1494 1494 Size: 5567 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1441 1473 4706 4738 1441 Size: 32 3233 32 2270 Fragments arranged by size: 3233 2270 32 32 PpuMI rG'GwC_Cy Cuts at: 1327 2079 1327 Size: 752 4815 PshAI GACnn'nnGTC Cuts at: 2024 2024 Size: 5567 PspOMI G'GGCC_C Cuts at: 1855 1855 Size: 5567 PstI C_TGCA'G Cuts at: 272 2019 2235 2614 3299 272 Size: 1747 216 379 685 2540 Fragments arranged by size: 2540 1747 685 379 216 PvuI CG_AT'CG Cuts at: 104 104 Size: 5567 PvuII CAG'CTG Cuts at: 138 703 2127 2230 2992 3352 138 Size: 565 1424 103 762 360 2353 Fragments arranged by size: 2353 1424 762 565 360 103 RsrII CG'GwC_CG Cuts at: 3762 3762 Size: 5567 SacI G_AGCT'C Cuts at: 1980 2465 2581 1980 Size: 485 116 4966 Fragments arranged by size: 4966 485 116 SacII CC_GC'GG Cuts at: 1814 1814 Size: 5567 SalI G'TCGA_C Cuts at: 2469 2604 2469 Size: 135 5432 SapI GCTCTTCn'nnn_ Cuts at: 3590 3800 3590 Size: 210 5357 SbfI CC_TGCA'GG Cuts at: 2614 2614 Size: 5567 ScaI AGT'ACT Cuts at: 1054 5084 1054 Size: 4030 1537 SexAI A'CCwGG_T Cuts at: 568 568 Size: 5567 SfcI C'TryA_G Cuts at: 33 268 1512 2015 2231 2420 2610 3295 4288 Size: 235 1244 503 216 189 190 685 993 Cuts at: 4288 4479 33 Size: 191 1121 Fragments arranged by size: 1244 1121 993 685 503 235 216 191 190 189 SfoI GGC'GCC Cuts at: 1555 1989 2999 3247 1555 Size: 434 1010 248 3875 Fragments arranged by size: 3875 1010 434 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5567 SmaI CCC'GGG Cuts at: 2589 2589 Size: 5567 SmlI C'TyrA_G Cuts at: 946 2722 3637 4129 4391 946 Size: 1776 915 492 262 2122 Fragments arranged by size: 2122 1776 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5567 SphI G_CATG'C Cuts at: 132 1429 2620 3120 132 Size: 1297 1191 500 2579 Fragments arranged by size: 2579 1297 1191 500 SspI AAT'ATT Cuts at: 510 510 Size: 5567 StuI AGG'CCT Cuts at: 844 844 Size: 5567 StyI C'CwwG_G Cuts at: 629 1220 2075 2204 2701 3055 629 Size: 591 855 129 497 354 3141 Fragments arranged by size: 3141 855 591 497 354 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1642 1668 2088 3150 3283 3742 3951 1642 Size: 26 420 1062 133 459 209 3258 Fragments arranged by size: 3258 1062 459 420 209 133 26 TatI w'GTAC_w Cuts at: 710 1052 1488 5082 710 Size: 342 436 3594 1195 Fragments arranged by size: 3594 1195 436 342 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 775 807 1191 1223 2063 2095 5084 5116 775 Size: 32 384 32 840 32 2989 32 1226 Fragments arranged by size: 2989 1226 840 384 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3364 3364 Size: 5567 XbaI T'CTAG_A Cuts at: 2598 2598 Size: 5567 XcmI CCAnnnn_n'nnnnTGG Cuts at: 150 543 3062 150 Size: 393 2519 2655 Fragments arranged by size: 2655 2519 393 XmaI C'CCGG_G Cuts at: 2587 2587 Size: 5567 XmnI GAAnn'nnTTC Cuts at: 118 191 118 Size: 73 5494 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AscI AvrII BaeI BfrBI BmtI BsiWI BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI NruI NsiI PacI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI