Restriction map for pF1KA0672
 (Circular) (Six-base) MAPSORT of: pF1KA0672.seq  Check: 4819  from: 1  to: 5567



pF1KA0672  5567 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AccI GT'mk_AC

Cuts at:   2419    2470    2605    2419 
   Size:         51     135    5381

  Fragments arranged by size: 

                5381     135      51
 
Acc65I G'GTAC_C

Cuts at:   2583    2583 
   Size:       5567
 
AclI AA'CG_TT

Cuts at:   5407    5407 
   Size:       5567
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    181     578    1024    1171    3392    3824    4571    4839     181 
   Size:        397     446     147    2221     432     747     268     909

  Fragments arranged by size: 

                2221     909     747     446     432     397     268     147
            
 
AfeI AGC'GCT

Cuts at:    301     301 
   Size:       5567
 
AflIII A'CryG_T

Cuts at:   1491    2919    4023    5000    1491 
   Size:       1428    1104     977    2058

  Fragments arranged by size: 

                2058    1428    1104     977
 
AgeI A'CCGG_T

Cuts at:   3955    3955 
   Size:       5567
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1403    1435    1441    1473    1403 
   Size:         32       6      32    5497

  Fragments arranged by size: 

                5497      32      32       6
 
AlwNI CAG_nnn'CTG

Cuts at:    292     339     432    1800    1903    2518    2872    4439     292 
   Size:         47      93    1368     103     615     354    1567    1420

  Fragments arranged by size: 

                1567    1420    1368     615     354     103      93      47
            
 
ApaI G_GGCC'C

Cuts at:   1859    1859 
   Size:       5567
 
ApaLI G'TGCA_C

Cuts at:   4337    4337 
   Size:       5567
 
ApoI r'AATT_y

Cuts at:   1255    2571    4920    5176    1255 
   Size:       1316    2349     256    1646

  Fragments arranged by size: 

                2349    1646    1316     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5567
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5567
 
AvaI C'yCGr_G

Cuts at:   1561    1858    2211    2464    2587    1561 
   Size:        297     353     253     123    4541

  Fragments arranged by size: 

                4541     353     297     253     123
 
BamHI G'GATC_C

Cuts at:   1148    2592    1148 
   Size:       1444    4123
 
BanI G'GyrC_C

Cuts at:    625    1553    1987    2061    2583    2997    3245    3280     625 
   Size:        928     434      74     522     414     248      35    2912

  Fragments arranged by size: 

                2912     928     522     434     414     248      74      35
            
 
BanII G_rGCy'C

Cuts at:    476    1859    1968    1980    2465    2581    3611     476 
   Size:       1383     109      12     485     116    1030    2432

  Fragments arranged by size: 

                2432    1383    1030     485     116     109      12
 
BbeI G_GCGC'C

Cuts at:   1557    1991    3001    3249    1557 
   Size:        434    1010     248    3875

  Fragments arranged by size: 

                3875    1010     434     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    194     584     194 
   Size:        390    5177
 
BbvCI CC'TCA_GC

Cuts at:   1582    2330    1582 
   Size:        748    4819
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    106     140    1747    1781    2541    2575     106 
   Size:         34    1607      34     760      34    3098

  Fragments arranged by size: 

                3098    1607     760      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3465    4226    5567    3465 
   Size:        761    1341    3465

  Fragments arranged by size: 

                3465    1341     761
 
BclI T'GATC_A

Cuts at:   3087    3087 
   Size:       5567
 
BglI GCCn_nnn'nGGC

Cuts at:   1762    1762 
   Size:       5567
 
BglII A'GATC_T

Cuts at:   2424    3082    5119    2424 
   Size:        658    2037    2872

  Fragments arranged by size: 

                2872    2037     658
 
BlpI GC'TnA_GC

Cuts at:   1979    2679    1979 
   Size:        700    4867
 
Bme1580I G_kGCm'C

Cuts at:   1859    1939    3192    3285    4341    1859 
   Size:         80    1253      93    1056    3085

  Fragments arranged by size: 

                3085    1253    1056      93      80
 
BmgBI CAC'GTC

Cuts at:    465     465 
   Size:       5567
 
BmrI ACTGGGnnnn_n'

Cuts at:   1298    1686    2128    2779    2860    3195    5339    1298 
   Size:        388     442     651      81     335    2144    1526

  Fragments arranged by size: 

                2144    1526     651     442     388     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2207    2333    3934    2207 
   Size:        126    1601    3840

  Fragments arranged by size: 

                3840    1601     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    235     898    1630    1831    1880    1911    1994    2512     235 
   Size:        663     732     201      49      31      83     518    3290

  Fragments arranged by size: 

                3290     732     663     518     201      83      49      31
            
 
Bpu10I CC'TnA_GC

Cuts at:    425    1582    2297    2330     425 
   Size:       1157     715      33    3662

  Fragments arranged by size: 

                3662    1157     715      33
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    967    2743    3622    4114    4412     967 
   Size:       1776     879     492     298    2122

  Fragments arranged by size: 

                2122    1776     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2304    5271    2304 
   Size:       2967    2600
 
BsaAI yAC'GTr

Cuts at:   1494    2920    1494 
   Size:       1426    4141
 
BsaBI GATnn'nnATC

Cuts at:      6    3081    5118       6 
   Size:       3075    2037     455

  Fragments arranged by size: 

                3075    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1554    1988    2998    3246    3948    5444    1554 
   Size:        434    1010     248     702    1496    1677

  Fragments arranged by size: 

                1677    1496    1010     702     434     248
 
BsaWI w'CCGG_w

Cuts at:   2039    2756    3277    3955    4229    4376    4787    2039 
   Size:        717     521     678     274     147     411    2819

  Fragments arranged by size: 

                2819     717     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    286     316    1319    1349    1441    1471    2527    2557     286 
   Size:         30    1003      30      92      30    1056      30    3296

  Fragments arranged by size: 

                3296    1056    1003      92      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    895     979    1686    2228    2318    2551    2554     895 
   Size:         84     707     542      90     233       3    3908

  Fragments arranged by size: 

                3908     707     542     233      90      84       3
 
BseYI C'CCAG_C

Cuts at:   1996    2002    2158    2226    2992    3750    4327    4823    1996 
   Size:          6     156      68     766     758     577     496    2740

  Fragments arranged by size: 

                2740     766     758     577     496     156      68       6
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1066    1889    1967    1066 
   Size:        823      78    4666

  Fragments arranged by size: 

                4666     823      78
 
BsiEI CG_ry'CG

Cuts at:    104     903    1703    1707    3109    3155    4363     104 
   Size:        799     800       4    1402      46    1208    1308

  Fragments arranged by size: 

                1402    1308    1208     800     799      46       4
 
BsiHKAI G_wGCw'C

Cuts at:    887    1980    2465    2498    2554    2581    3359    3549    4341 
   Size:       1093     485      33      56      27     778     190     792

Cuts at:   4341     887 
   Size:       2113

  Fragments arranged by size: 

                2113    1093     792     778     485     190      56      33
                  27
 
BsmI GAATG_Cn'

Cuts at:   1206    1206 
   Size:       5567
 
BsmBI CGTCTCn'nnnn_

Cuts at:    400     455     400 
   Size:         55    5512
 
Bsp1286I G_dGCh'C

Cuts at:    476     887    1859    1939    1968    1980    2465    2498    2554 
   Size:        411     972      80      29      12     485      33      56

Cuts at:   2554    2581    3192    3285    3359    3549    3611    4341     476 
   Size:         27     611      93      74     190      62     730    1702

  Fragments arranged by size: 

                1702     972     730     611     485     411     190      93
                  80      74      62      56      33      29      27      12
            
 
BspEI T'CCGG_A

Cuts at:   2039    4787    2039 
   Size:       2748    2819
 
BspHI T'CATG_A

Cuts at:   4743    4743 
   Size:       5567
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2617    2831    3133    3514    2617 
   Size:        214     302     381    4670

  Fragments arranged by size: 

                4670     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3859    3913    5397    5562      50 
   Size:       3809      54    1484     165      55

  Fragments arranged by size: 

                3809    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3479    3479 
   Size:       5567
 
BsrFI r'CCGG_y

Cuts at:   1550    1574    1702    3565    3746    3955    1550 
   Size:         24     128    1863     181     209    3162

  Fragments arranged by size: 

                3162    1863     209     181     128      24
 
BsrGI T'GTAC_A

Cuts at:    710     710 
   Size:       5567
 
BssSI C'ACGA_G

Cuts at:   3838    4196    3838 
   Size:        358    5209
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2944    2944 
   Size:       5567
 
BstBI TT'CG_AA

Cuts at:   3928    3928 
   Size:       5567
 
BstXI CCAn_nnnn'nTGG

Cuts at:    675    1311    2119     675 
   Size:        636     808    4123

  Fragments arranged by size: 

                4123     808     636
 
BstYI r'GATC_y

Cuts at:      1    1148    2424    2592    3059    3082    3417    3663    4675 
   Size:       1147    1276     168     467      23     335     246    1012

Cuts at:   4675    4761    4773    5119       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1276    1147    1012     467     449     346     335     246
                 168      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    643    1633    2192     643 
   Size:        990     559    4018

  Fragments arranged by size: 

                4018     990     559
 
BtgI C'CryG_G

Cuts at:   1592    1811    2372    1592 
   Size:        219     561    4787

  Fragments arranged by size: 

                4787     561     219
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3517    3698    3861    3517 
   Size:        181     163    5223

  Fragments arranged by size: 

                5223     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2810    2810 
   Size:       5567
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1661    1696    1661 
   Size:         35    5532
 
DraI TTT'AAA

Cuts at:   2566    2566 
   Size:       5567
 
DrdI GACnn_nn'nnGTC

Cuts at:    588    1383    3273    4131     588 
   Size:        795    1890     858    2024

  Fragments arranged by size: 

                2024    1890     858     795
 
EaeI y'GGCC_r

Cuts at:    140     722     900    1342    1700    2088    2121    3152    3326 
   Size:        582     178     442     358     388      33    1031     174

Cuts at:   3326    3717    3744    5089     140 
   Size:        391      27    1345     618

  Fragments arranged by size: 

                1345    1031     618     582     442     391     388     358
                 178     174      33      27
 
EagI C'GGCC_G

Cuts at:    900    1700    3152     900 
   Size:        800    1452    3315

  Fragments arranged by size: 

                3315    1452     800
 
EarI CTCTTCn'nnn_

Cuts at:    653     979    1683    3590    3800     653 
   Size:        326     704    1907     210    2420

  Fragments arranged by size: 

                2420    1907     704     326     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    505    2467    3775    4085    4231    5377     505 
   Size:       1962    1308     310     146    1146     695

  Fragments arranged by size: 

                1962    1308    1146     695     310     146
 
EcoICRI GAG'CTC

Cuts at:   1978    2463    2579    1978 
   Size:        485     116    4966

  Fragments arranged by size: 

                4966     485     116
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    181     235     578     898    1024    1171    1630    1831    1880 
   Size:         54     343     320     126     147     459     201      49

Cuts at:   1880    1911    1994    2512    3392    3824    4571    4839     181 
   Size:         31      83     518     880     432     747     268     909

  Fragments arranged by size: 

                 909     880     747     518     459     432     343     320
                 268     201     147     126      83      54      49      31
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    196     196 
   Size:       5567
 
EcoO109I rG'GnC_Cy

Cuts at:   1327    2079    2379    2706    1327 
   Size:        752     300     327    4188

  Fragments arranged by size: 

                4188     752     327     300
 
EcoRI G'AATT_C

Cuts at:   2571    2571 
   Size:       5567
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    211     879    2100    3586     211 
   Size:        668    1221    1486    2192

  Fragments arranged by size: 

                2192    1486    1221     668
 
FspI TGC'GCA

Cuts at:   3348    3348 
   Size:       5567
 
HaeII r_GCGC'y

Cuts at:    303    1557    1991    3001    3249    4271    5250     303 
   Size:       1254     434    1010     248    1022     979     620

  Fragments arranged by size: 

                1254    1022    1010     979     620     434     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    286     318     682     714    1319    1351    2383    2415    2431 
   Size:         32     364      32     605      32    1032      32      16

Cuts at:   2431    2452    2463    2484    2527    2559    3409    3439    3441 
   Size:         21      11      21      43      32     850      30       2

Cuts at:   3441    3471     286 
   Size:         30    2382

  Fragments arranged by size: 

                2382    1032     850     605     364      43      32      32
                  32      32      32      30      30      21      21      16
                  11       2
 
HincII GTy'rAC

Cuts at:   2471    2606    2471 
   Size:        135    5432
 
HindIII A'AGCT_T

Cuts at:   1183    1183 
   Size:       5567
 
KasI G'GCGC_C

Cuts at:   1553    1987    2997    3245    1553 
   Size:        434    1010     248    3875

  Fragments arranged by size: 

                3875    1010     434     248
 
KpnI G_GTAC'C

Cuts at:   2587    2587 
   Size:       5567
 
MfeI C'AATT_G

Cuts at:   1073    1316    1073 
   Size:        243    5324
 
MluI A'CGCG_T

Cuts at:   5000    5000 
   Size:       5567
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1687    4238    4422    1687 
   Size:       2551     184    2832

  Fragments arranged by size: 

                2832    2551     184
 
MscI TGG'CCA

Cuts at:    142     724    1344    2090    2123    3328     142 
   Size:        582     620     746      33    1205    2381

  Fragments arranged by size: 

                2381    1205     746     620     582      33
 
MslI CAynn'nnrTG

Cuts at:   1654    3556    3683    1654 
   Size:       1902     127    3538

  Fragments arranged by size: 

                3538    1902     127
 
MspA1I CmG'CkG

Cuts at:    138     703     876     899    1785    1813    2119    2127    2230 
   Size:        565     173      23     886      28     306       8     103

Cuts at:   2230    2678    2992    3352    4365    4610     138 
   Size:        448     314     360    1013     245    1095

  Fragments arranged by size: 

                1095    1013     886     565     448     360     314     306
                 245     173     103      28      23       8
 
NaeI GCC'GGC

Cuts at:   1576    3748    1576 
   Size:       2172    3395
 
NarI GG'CG_CC

Cuts at:   1554    1988    2998    3246    1554 
   Size:        434    1010     248    3875

  Fragments arranged by size: 

                3875    1010     434     248
 
NgoMIV G'CCGG_C

Cuts at:   1574    3746    1574 
   Size:       2172    3395
 
NspI r_CATG'y

Cuts at:    132     266    1429    2400    2418    2502    2620    3120    4027 
   Size:        134    1163     971      18      84     118     500     907

Cuts at:   4027     132 
   Size:       1672

  Fragments arranged by size: 

                1672    1163     971     907     500     134     118      84
                  18
 
PasI CC'CwG_GG

Cuts at:   1444    2404    1444 
   Size:        960    4607
 
PciI A'CATG_T

Cuts at:   4023    4023 
   Size:       5567
 
PflMI CCAn_nnn'nTGG

Cuts at:    153    1650     153 
   Size:       1497    4070
 
PfoI T'CCnGG_A

Cuts at:   1166    1166 
   Size:       5567
 
PmeI GTTT'AAAC

Cuts at:   2566    2566 
   Size:       5567
 
PmlI CAC'GTG

Cuts at:   1494    1494 
   Size:       5567
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1441    1473    4706    4738    1441 
   Size:         32    3233      32    2270

  Fragments arranged by size: 

                3233    2270      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1327    2079    1327 
   Size:        752    4815
 
PshAI GACnn'nnGTC

Cuts at:   2024    2024 
   Size:       5567
 
PspOMI G'GGCC_C

Cuts at:   1855    1855 
   Size:       5567
 
PstI C_TGCA'G

Cuts at:    272    2019    2235    2614    3299     272 
   Size:       1747     216     379     685    2540

  Fragments arranged by size: 

                2540    1747     685     379     216
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5567
 
PvuII CAG'CTG

Cuts at:    138     703    2127    2230    2992    3352     138 
   Size:        565    1424     103     762     360    2353

  Fragments arranged by size: 

                2353    1424     762     565     360     103
 
RsrII CG'GwC_CG

Cuts at:   3762    3762 
   Size:       5567
 
SacI G_AGCT'C

Cuts at:   1980    2465    2581    1980 
   Size:        485     116    4966

  Fragments arranged by size: 

                4966     485     116
 
SacII CC_GC'GG

Cuts at:   1814    1814 
   Size:       5567
 
SalI G'TCGA_C

Cuts at:   2469    2604    2469 
   Size:        135    5432
 
SapI GCTCTTCn'nnn_

Cuts at:   3590    3800    3590 
   Size:        210    5357
 
SbfI CC_TGCA'GG

Cuts at:   2614    2614 
   Size:       5567
 
ScaI AGT'ACT

Cuts at:   1054    5084    1054 
   Size:       4030    1537
 
SexAI A'CCwGG_T

Cuts at:    568     568 
   Size:       5567
 
SfcI C'TryA_G

Cuts at:     33     268    1512    2015    2231    2420    2610    3295    4288 
   Size:        235    1244     503     216     189     190     685     993

Cuts at:   4288    4479      33 
   Size:        191    1121

  Fragments arranged by size: 

                1244    1121     993     685     503     235     216     191
                 190     189
 
SfoI GGC'GCC

Cuts at:   1555    1989    2999    3247    1555 
   Size:        434    1010     248    3875

  Fragments arranged by size: 

                3875    1010     434     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5567
 
SmaI CCC'GGG

Cuts at:   2589    2589 
   Size:       5567
 
SmlI C'TyrA_G

Cuts at:    946    2722    3637    4129    4391     946 
   Size:       1776     915     492     262    2122

  Fragments arranged by size: 

                2122    1776     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5567
 
SphI G_CATG'C

Cuts at:    132    1429    2620    3120     132 
   Size:       1297    1191     500    2579

  Fragments arranged by size: 

                2579    1297    1191     500
 
SspI AAT'ATT

Cuts at:    510     510 
   Size:       5567
 
StuI AGG'CCT

Cuts at:    844     844 
   Size:       5567
 
StyI C'CwwG_G

Cuts at:    629    1220    2075    2204    2701    3055     629 
   Size:        591     855     129     497     354    3141

  Fragments arranged by size: 

                3141     855     591     497     354     129
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1642    1668    2088    3150    3283    3742    3951    1642 
   Size:         26     420    1062     133     459     209    3258

  Fragments arranged by size: 

                3258    1062     459     420     209     133      26
 
TatI w'GTAC_w

Cuts at:    710    1052    1488    5082     710 
   Size:        342     436    3594    1195

  Fragments arranged by size: 

                3594    1195     436     342
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    775     807    1191    1223    2063    2095    5084    5116     775 
   Size:         32     384      32     840      32    2989      32    1226

  Fragments arranged by size: 

                2989    1226     840     384      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   3364    3364 
   Size:       5567
 
XbaI T'CTAG_A

Cuts at:   2598    2598 
   Size:       5567
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    150     543    3062     150 
   Size:        393    2519    2655

  Fragments arranged by size: 

                2655    2519     393
 
XmaI C'CCGG_G

Cuts at:   2587    2587 
   Size:       5567
 
XmnI GAAnn'nnTTC

Cuts at:    118     191     118 
   Size:         73    5494
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AscI    AvrII
     BaeI    BfrBI     BmtI    BsiWI   BssHII   BstEII  BstZ17I     ClaI
   DraIII    EcoRV     FseI    FspAI     HpaI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PsiI    PspXI     PsrI    SanDI
     SfiI    SgrAI    SnaBI     SrfI     SwaI     XhoI     ZraI
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