Restriction map for
pF1KA0363
(Linear) (Six-base) MAPSORT of: KIAA0363.seq Check: 2434 from: 1 to: 4407
KIAA0363.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
Acc65I G'GTAC_C
Cuts at: 0 3418 4407
Size: 3418 989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1631 1751 1871 1991 2111 2231 2351
Size: 467 1164 120 120 120 120 120 120
Cuts at: 2351 2471 2992 3170 4407
Size: 120 521 178 1237
Fragments arranged by size:
1237 1164 521 467 178 120 120 120
120 120 120 120
AfeI AGC'GCT
Cuts at: 0 134 4407
Size: 134 4273
AhdI GACnn_n'nnGTC
Cuts at: 0 789 4407
Size: 789 3618
AleI CACnn'nnGTG
Cuts at: 0 1594 1714 1834 1954 2074 2194 2314 2434
Size: 1594 120 120 120 120 120 120 120
Cuts at: 2434 2900 3379 4102 4407
Size: 466 479 723 305
Fragments arranged by size:
1594 723 479 466 305 120 120 120
120 120 120 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3349 4407
Size: 3349 1058
AlwNI CAG_nnn'CTG
Cuts at: 0 33 229 325 813 2599 2656 2746 3218
Size: 33 196 96 488 1786 57 90 472
Cuts at: 3218 3591 4407
Size: 373 816
Fragments arranged by size:
1786 816 488 472 373 196 96 90
57 33
ApaI G_GGCC'C
Cuts at: 0 299 3400 3722 3807 4407
Size: 299 3101 322 85 600
Fragments arranged by size:
3101 600 322 299 85
ApaLI G'TGCA_C
Cuts at: 0 4147 4407
Size: 4147 260
AvaI C'yCGr_G
Cuts at: 0 178 369 3757 3800 3813 4223 4407
Size: 178 191 3388 43 13 410 184
Fragments arranged by size:
3388 410 191 184 178 43 13
BamHI G'GATC_C
Cuts at: 0 741 4407
Size: 741 3666
BanI G'GyrC_C
Cuts at: 0 3325 3418 4176 4407
Size: 3325 93 758 231
Fragments arranged by size:
3325 758 231 93
BanII G_rGCy'C
Cuts at: 0 71 299 376 539 947 3085 3131 3400
Size: 71 228 77 163 408 2138 46 269
Cuts at: 3400 3455 3512 3722 3730 3749 3807 3814 4087
Size: 55 57 210 8 19 58 7 273
Cuts at: 4087 4190 4356 4407
Size: 103 166 51
Fragments arranged by size:
2138 408 273 269 228 210 166 163
103 77 71 58 57 55 51 46
19 8 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 14 991 3176 3494 3506 3797 4068 4407
Size: 14 977 2185 318 12 291 271 339
Fragments arranged by size:
2185 977 339 318 291 271 14 12
BbvCI CC'TCA_GC
Cuts at: 0 427 1295 3141 3637 4191 4407
Size: 427 868 1846 496 554 216
Fragments arranged by size:
1846 868 554 496 427 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 835 856 869 890 4407
Size: 835 21 13 21 3517
Fragments arranged by size:
3517 835 21 21 13
BglI GCCn_nnn'nGGC
Cuts at: 0 593 1057 2611 3110 3157 3734 3976 4351
Size: 593 464 1554 499 47 577 242 375
Cuts at: 4351 4407
Size: 56
Fragments arranged by size:
1554 593 577 499 464 375 242 56
47
BglII A'GATC_T
Cuts at: 0 1322 1554 1794 1914 2034 2154 2274 2394
Size: 1322 232 240 120 120 120 120 120
Cuts at: 2394 3683 4407
Size: 1289 724
Fragments arranged by size:
1322 1289 724 240 232 120 120 120
120 120
BlpI GC'TnA_GC
Cuts at: 0 326 2615 2912 4407
Size: 326 2289 297 1495
Fragments arranged by size:
2289 1495 326 297
Bme1580I G_kGCm'C
Cuts at: 0 158 299 2586 3400 3722 3807 4151 4181
Size: 158 141 2287 814 322 85 344 30
Cuts at: 4181 4407
Size: 226
Fragments arranged by size:
2287 814 344 322 226 158 141 85
30
BmgBI CAC'GTC
Cuts at: 0 95 791 3242 4407
Size: 95 696 2451 1165
Fragments arranged by size:
2451 1165 696 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 3286 3308 3608 4407
Size: 3286 22 300 799
Fragments arranged by size:
3286 799 300 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2838 3631 3810 4407
Size: 2838 793 179 597
Fragments arranged by size:
2838 793 597 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 952 1186 2962 3469 3805 4255 4407
Size: 85 867 234 1776 507 336 450 152
Fragments arranged by size:
1776 867 507 450 336 234 152 85
Bpu10I CC'TnA_GC
Cuts at: 0 136 427 1028 1295 2813 3079 3141 3637
Size: 136 291 601 267 1518 266 62 496
Cuts at: 3637 4191 4407
Size: 554 216
Fragments arranged by size:
1518 601 554 496 291 267 266 216
136 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3725 4407
Size: 3725 682
BsaI GGTCTCn'nnnn_
Cuts at: 0 2929 3922 4407
Size: 2929 993 485
Fragments arranged by size:
2929 993 485
BsaBI GATnn'nnATC
Cuts at: 0 4027 4407
Size: 4027 380
BsaHI Gr'CG_yC
Cuts at: 0 308 4407
Size: 308 4099
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 30 60 559 589 886 916 4407
Size: 30 30 499 30 297 30 3491
Fragments arranged by size:
3491 499 297 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 38 103 567 742 793 873 1006 1009
Size: 38 65 464 175 51 80 133 3
Cuts at: 1009 1055 1394 2674 3520 3625 3698 3869 3940
Size: 46 339 1280 846 105 73 171 71
Cuts at: 3940 4270 4273 4276 4407
Size: 330 3 3 131
Fragments arranged by size:
1280 846 464 339 330 175 171 133
131 105 80 73 71 65 51 46
38 3 3 3
BseYI C'CCAG_C
Cuts at: 0 401 470 519 766 1237 1601 1721 1841
Size: 401 69 49 247 471 364 120 120
Cuts at: 1841 1961 2081 2201 2321 2441 2746 2830 3084
Size: 120 120 120 120 120 305 84 254
Cuts at: 3084 3257 3370 3427 3456 3729 3909 3990 4086
Size: 173 113 57 29 273 180 81 96
Cuts at: 4086 4139 4407
Size: 53 268
Fragments arranged by size:
471 401 364 305 273 268 254 247
180 173 120 120 120 120 120 120
120 113 96 84 81 69 57 53
49 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 3230 3910 4407
Size: 499 2731 680 497
Fragments arranged by size:
2731 680 499 497
BsiEI CG_ry'CG
Cuts at: 0 389 4407
Size: 389 4018
BsiHKAI G_wGCw'C
Cuts at: 0 4151 4407
Size: 4151 256
BsmI GAATG_Cn'
Cuts at: 0 2673 3822 4407
Size: 2673 1149 585
Fragments arranged by size:
2673 1149 585
BsmBI CGTCTCn'nnnn_
Cuts at: 0 85 4407
Size: 85 4322
Bsp1286I G_dGCh'C
Cuts at: 0 71 158 299 376 539 947 2586 3085
Size: 71 87 141 77 163 408 1639 499
Cuts at: 3085 3131 3400 3455 3512 3722 3730 3749 3807
Size: 46 269 55 57 210 8 19 58
Cuts at: 3807 3814 4087 4151 4181 4190 4356 4407
Size: 7 273 64 30 9 166 51
Fragments arranged by size:
1639 499 408 273 269 210 166 163
141 87 77 71 64 58 57 55
51 46 30 19 9 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1176 4407
Size: 1176 3231
BsrBI CCG'CTC
Cuts at: 0 1001 4407
Size: 1001 3406
BsrDI GCAATG_nn'
Cuts at: 0 1295 3986 4407
Size: 1295 2691 421
Fragments arranged by size:
2691 1295 421
BsrFI r'CCGG_y
Cuts at: 0 2817 4407
Size: 2817 1590
BsrGI T'GTAC_A
Cuts at: 0 932 4407
Size: 932 3475
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2581 3687 4407
Size: 2581 1106 720
Fragments arranged by size:
2581 1106 720
BstXI CCAn_nnnn'nTGG
Cuts at: 0 151 3672 4407
Size: 151 3521 735
Fragments arranged by size:
3521 735 151
BstYI r'GATC_y
Cuts at: 0 741 1322 1554 1794 1914 2034 2154 2274
Size: 741 581 232 240 120 120 120 120
Cuts at: 2274 2394 3683 4407
Size: 120 1289 724
Fragments arranged by size:
1289 741 724 581 240 232 120 120
120 120 120
Bsu36I CC'TnA_GG
Cuts at: 0 11 465 2523 2637 2657 3056 4407
Size: 11 454 2058 114 20 399 1351
Fragments arranged by size:
2058 1351 454 399 114 20 11
BtgI C'CryG_G
Cuts at: 0 38 300 494 1052 1283 2609 3380 3656
Size: 38 262 194 558 231 1326 771 276
Cuts at: 3656 3834 4407
Size: 178 573
Fragments arranged by size:
1326 771 573 558 276 262 231 194
178 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3918 4044 4407
Size: 3918 126 363
Fragments arranged by size:
3918 363 126
BtsI GCAGTG_nn'
Cuts at: 0 695 1157 1343 3294 3898 3927 3963 4407
Size: 695 462 186 1951 604 29 36 444
Fragments arranged by size:
1951 695 604 462 444 186 36 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1559 1594 1679 1714 1799 1834 1919 1954
Size: 1559 35 85 35 85 35 85 35
Cuts at: 1954 2039 2074 2159 2194 2279 2314 2399 2434
Size: 85 35 85 35 85 35 85 35
Cuts at: 2434 4407
Size: 1973
Fragments arranged by size:
1973 1559 85 85 85 85 85 85
85 35 35 35 35 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 1391 4407
Size: 1391 3016
EaeI y'GGCC_r
Cuts at: 0 56 386 620 2546 2603 3149 4407
Size: 56 330 234 1926 57 546 1258
Fragments arranged by size:
1926 1258 546 330 234 57 56
EagI C'GGCC_G
Cuts at: 0 386 4407
Size: 386 4021
EarI CTCTTCn'nnn_
Cuts at: 0 414 719 1124 1607 1727 1847 1967 2087
Size: 414 305 405 483 120 120 120 120
Cuts at: 2087 2207 2327 2447 4241 4247 4262 4407
Size: 120 120 120 1794 6 15 145
Fragments arranged by size:
1794 483 414 405 305 145 120 120
120 120 120 120 120 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 754 4407
Size: 754 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 467 952 1186 1631 1751 1871 1991
Size: 85 382 485 234 445 120 120 120
Cuts at: 1991 2111 2231 2351 2471 2962 2992 3170 3469
Size: 120 120 120 120 491 30 178 299
Cuts at: 3469 3805 4255 4407
Size: 336 450 152
Fragments arranged by size:
491 485 450 445 382 336 299 234
178 152 120 120 120 120 120 120
120 85 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 3358 3472 4407
Size: 10 3348 114 935
Fragments arranged by size:
3348 935 114 10
EcoO109I rG'GnC_Cy
Cuts at: 0 15 102 296 474 527 759 1085 1134
Size: 15 87 194 178 53 232 326 49
Cuts at: 1134 1497 1617 1737 1857 1977 2097 2217 2337
Size: 363 120 120 120 120 120 120 120
Cuts at: 2337 2457 2515 2641 2799 3027 3389 3397 3477
Size: 120 58 126 158 228 362 8 80
Cuts at: 3477 3719 3804 3906 4131 4220 4407
Size: 242 85 102 225 89 187
Fragments arranged by size:
363 362 326 242 232 228 225 194
187 178 158 126 120 120 120 120
120 120 120 120 102 89 87 85
80 58 53 49 15 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1153 4011 4407
Size: 1153 2858 396
Fragments arranged by size:
2858 1153 396
HaeII r_GCGC'y
Cuts at: 0 136 4407
Size: 136 4271
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 448 480 526 558 559 591
Size: 4 32 412 32 46 32 1 32
Cuts at: 591 775 807 856 880 886 888 912 918
Size: 184 32 49 24 6 2 24 6
Cuts at: 918 1426 1458 1518 1546 1550 1578 1638 1670
Size: 508 32 60 28 4 28 60 32
Cuts at: 1670 1758 1786 1790 1818 1878 1906 1910 1938
Size: 88 28 4 28 60 28 4 28
Cuts at: 1938 1998 2026 2030 2058 2118 2146 2150 2178
Size: 60 28 4 28 60 28 4 28
Cuts at: 2178 2238 2266 2270 2298 2358 2386 2390 2418
Size: 60 28 4 28 60 28 4 28
Cuts at: 2418 2478 2510 4120 4152 4407
Size: 60 32 1610 32 255
Fragments arranged by size:
1610 508 412 255 184 88 60 60
60 60 60 60 60 60 49 46
32 32 32 32 32 32 32 32
32 28 28 28 28 28 28 28
28 28 28 28 28 28 28 24
24 6 6 4 4 4 4 4
4 4 4 2 1
HincII GTy'rAC
Cuts at: 0 4383 4407
Size: 4383 24
KpnI G_GTAC'C
Cuts at: 0 3422 4407
Size: 3422 985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1384 2739 4407
Size: 1384 1355 1668
Fragments arranged by size:
1668 1384 1355
MscI TGG'CCA
Cuts at: 0 622 2548 2605 3151 4407
Size: 622 1926 57 546 1256
Fragments arranged by size:
1926 1256 622 546 57
MslI CAynn'nnrTG
Cuts at: 0 1288 1594 1714 1834 1954 2074 2194 2314
Size: 1288 306 120 120 120 120 120 120
Cuts at: 2314 2434 2900 3379 4102 4407
Size: 120 466 479 723 305
Fragments arranged by size:
1288 723 479 466 306 305 120 120
120 120 120 120 120
MspA1I CmG'CkG
Cuts at: 0 401 938 1264 2737 3145 3562 3733 4162
Size: 401 537 326 1473 408 417 171 429
Cuts at: 4162 4407
Size: 245
Fragments arranged by size:
1473 537 429 417 408 401 326 245
171
NaeI GCC'GGC
Cuts at: 0 2819 4407
Size: 2819 1588
NcoI C'CATG_G
Cuts at: 0 494 1283 2609 3656 4407
Size: 494 789 1326 1047 751
Fragments arranged by size:
1326 1047 789 751 494
NgoMIV G'CCGG_C
Cuts at: 0 2817 4407
Size: 2817 1590
NspI r_CATG'y
Cuts at: 0 2879 4407
Size: 2879 1528
PasI CC'CwG_GG
Cuts at: 0 2587 3393 3807 4407
Size: 2587 806 414 600
Fragments arranged by size:
2587 806 600 414
PflMI CCAn_nnn'nTGG
Cuts at: 0 1237 4407
Size: 1237 3170
PfoI T'CCnGG_A
Cuts at: 0 860 968 2687 4407
Size: 860 108 1719 1720
Fragments arranged by size:
1720 1719 860 108
PpuMI rG'GwC_Cy
Cuts at: 0 527 759 1497 3027 3477 4131 4407
Size: 527 232 738 1530 450 654 276
Fragments arranged by size:
1530 738 654 527 450 276 232
PshAI GACnn'nnGTC
Cuts at: 0 3000 4407
Size: 3000 1407
PspOMI G'GGCC_C
Cuts at: 0 295 3396 3718 3803 4407
Size: 295 3101 322 85 604
Fragments arranged by size:
3101 604 322 295 85
PstI C_TGCA'G
Cuts at: 0 567 809 879 1025 1187 1350 1467 1587
Size: 567 242 70 146 162 163 117 120
Cuts at: 1587 1707 1827 1947 2067 2187 2307 2427 2700
Size: 120 120 120 120 120 120 120 273
Cuts at: 2700 2742 3341 4407
Size: 42 599 1066
Fragments arranged by size:
1066 599 567 273 242 163 162 146
120 120 120 120 120 120 120 120
117 70 42
PvuII CAG'CTG
Cuts at: 0 401 2737 3145 3733 4162 4407
Size: 401 2336 408 588 429 245
Fragments arranged by size:
2336 588 429 408 401 245
SanDI GG'GwC_CC
Cuts at: 0 527 4407
Size: 527 3880
SexAI A'CCwGG_T
Cuts at: 0 2708 4407
Size: 2708 1699
SfcI C'TryA_G
Cuts at: 0 563 805 875 1021 1183 1346 1457 1463
Size: 563 242 70 146 162 163 111 6
Cuts at: 1463 1583 1703 1823 1943 2063 2183 2303 2423
Size: 120 120 120 120 120 120 120 120
Cuts at: 2423 2681 2696 2738 3337 4407
Size: 258 15 42 599 1070
Fragments arranged by size:
1070 599 563 258 242 163 162 146
120 120 120 120 120 120 120 120
111 70 42 15 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 593 3157 4407
Size: 593 2564 1250
Fragments arranged by size:
2564 1250 593
SmaI CCC'GGG
Cuts at: 0 4225 4407
Size: 4225 182
SmlI C'TyrA_G
Cuts at: 0 3740 4407
Size: 3740 667
StuI AGG'CCT
Cuts at: 0 1199 2635 2862 2969 3104 4407
Size: 1199 1436 227 107 135 1303
Fragments arranged by size:
1436 1303 1199 227 135 107
StyI C'CwwG_G
Cuts at: 0 494 1013 1283 2609 2702 2755 3321 3359
Size: 494 519 270 1326 93 53 566 38
Cuts at: 3359 3644 3656 4196 4340 4407
Size: 285 12 540 144 67
Fragments arranged by size:
1326 566 540 519 494 285 270 144
93 67 53 38 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 129 533 4230 4407
Size: 129 404 3697 177
Fragments arranged by size:
3697 404 177 129
TatI w'GTAC_w
Cuts at: 0 932 3273 4407
Size: 932 2341 1134
Fragments arranged by size:
2341 1134 932
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 30 62 1377 1409 2511 2543 4407
Size: 30 32 1315 32 1102 32 1864
Fragments arranged by size:
1864 1315 1102 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 0 976 1041 2710 4378 4407
Size: 976 65 1669 1668 29
Fragments arranged by size:
1669 1668 976 65 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2790 3378 3588 3730 4407
Size: 2790 588 210 142 677
Fragments arranged by size:
2790 677 588 210 142
XmaI C'CCGG_G
Cuts at: 0 4223 4407
Size: 4223 184
XmnI GAAnn'nnTTC
Cuts at: 0 1355 4407
Size: 1355 3052
Enzymes that do cut:
Acc65I AcuI AfeI AhdI AleI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SanDI SexAI SfcI SfiI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI
BclI BfrBI BmtI BsaAI BsaWI BsiWI BspEI BspHI
BssHII BssSI BstBI BstEII BstZ17I ClaI DraI DrdI
EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI ZraI