Restriction map for pF1KA0348
 (Circular) (Six-base) MAPSORT of: pF1KA0348.seq  Check: 629  from: 1  to: 7601



pF1KA0348  7601 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    990    3285    4151    4230    4560     990 
   Size:       2295     866      79     330    4031

  Fragments arranged by size: 

                4031    2295     866     330      79
 
AatII G_ACGT'C

Cuts at:   1574    1574 
   Size:       7601
 
AccI GT'mk_AC

Cuts at:   1574    4639    1574 
   Size:       3065    4536
 
Acc65I G'GTAC_C

Cuts at:   4101    4617    4101 
   Size:        516    7085
 
AclI AA'CG_TT

Cuts at:   7441    7441 
   Size:       7601
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    412     673     883    1000    1498    1924    3461    4460    5426 
   Size:        261     210     117     498     426    1537     999     966

Cuts at:   5426    5858    6211    6873     412 
   Size:        432     353     662    1140

  Fragments arranged by size: 

                1537    1140     999     966     662     498     432     426
                 353     261     210     117
 
AflIII A'CryG_T

Cuts at:    284    4953    6753    7034     284 
   Size:       4669    1800     281     851

  Fragments arranged by size: 

                4669    1800     851     281
 
AgeI A'CCGG_T

Cuts at:   3451    3451 
   Size:       7601
 
AhdI GACnn_n'nnGTC

Cuts at:   1664    3132    4461    1664 
   Size:       1468    1329    4804

  Fragments arranged by size: 

                4804    1468    1329
 
AleI CACnn'nnGTG

Cuts at:    367     767     367 
   Size:        400    7201
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    142    4238     142 
   Size:       4096    3505
 
AlwNI CAG_nnn'CTG

Cuts at:    388    2144    2913    4152    4906    6344     388 
   Size:       1756     769    1239     754    1438    1645

  Fragments arranged by size: 

                1756    1645    1438    1239     769     754
 
ApaI G_GGCC'C

Cuts at:   2767    3908    2767 
   Size:       1141    6460
 
ApaLI G'TGCA_C

Cuts at:   6439    6439 
   Size:       7601
 
ApoI r'AATT_y

Cuts at:    377    1675    1683    2362    2399    3181    4605    6954    7210 
   Size:       1298       8     679      37     782    1424    2349     256

Cuts at:   7210     377 
   Size:        768

  Fragments arranged by size: 

                2349    1424    1298     782     768     679     256      37
                   8
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       7601
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7601
 
AvaI C'yCGr_G

Cuts at:    483    1312    1803    2726    3214    3950    4107    4621     483 
   Size:        829     491     923     488     736     157     514    3463

  Fragments arranged by size: 

                3463     923     829     736     514     491     488     157
            
 
BamHI G'GATC_C

Cuts at:   1643    1694    4626    1643 
   Size:         51    2932    4618

  Fragments arranged by size: 

                4618    2932      51
 
BanI G'GyrC_C

Cuts at:    140    2104    2626    3499    3673    3774    4080    4101    4214 
   Size:       1964     522     873     174     101     306      21     113

Cuts at:   4214    4231    4294    4617    5031    5279    5314     140 
   Size:         17      63     323     414     248      35    2427

  Fragments arranged by size: 

                2427    1964     873     522     414     323     306     248
                 174     113     101      63      35      21      17
 
BanII G_rGCy'C

Cuts at:   1449    1491    2767    2852    3338    3740    3908    4408    4438 
   Size:         42    1276      85     486     402     168     500      30

Cuts at:   4438    4615    5645    1449 
   Size:        177    1030    3405

  Fragments arranged by size: 

                3405    1276    1030     500     486     402     177     168
                  85      42      30
 
BbeI G_GCGC'C

Cuts at:    144    2108    3778    4084    5035    5283     144 
   Size:       1964    1670     306     951     248    2462

  Fragments arranged by size: 

                2462    1964    1670     951     306     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1199    2077    2207    2313    3010    3161    3170    4230    1199 
   Size:        878     130     106     697     151       9    1060    4570

  Fragments arranged by size: 

                4570    1060     878     697     151     130     106       9
            
 
BbvCI CC'TCA_GC

Cuts at:    307     478    3554     307 
   Size:        171    3076    4354

  Fragments arranged by size: 

                4354    3076     171
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2704    2738    2704 
   Size:         34    7567
 
BciVI GTATCCnnnnn_n'

Cuts at:   5499    6555    7601    5499 
   Size:       1056    1046    5499

  Fragments arranged by size: 

                5499    1056    1046
 
BclI T'GATC_A

Cuts at:   1103    2367    2667    5121    1103 
   Size:       1264     300    2454    3583

  Fragments arranged by size: 

                3583    2454    1264     300
 
BglI GCCn_nnn'nGGC

Cuts at:    232     618    1088    3631     232 
   Size:        386     470    2543    4202

  Fragments arranged by size: 

                4202    2543     470     386
 
BglII A'GATC_T

Cuts at:   5116    7153    5116 
   Size:       2037    5564
 
BlpI GC'TnA_GC

Cuts at:   2463    4713    2463 
   Size:       2250    5351
 
Bme1580I G_kGCm'C

Cuts at:    614    2631    2767    3908    4097    4217    4234    5226    5319 
   Size:       2017     136    1141     189     120      17     992      93

Cuts at:   5319    6443     614 
   Size:       1124    1772

  Fragments arranged by size: 

                2017    1772    1141    1124     992     189     136     120
                  93      17
 
BmgBI CAC'GTC

Cuts at:    329     329 
   Size:       7601
 
BmrI ACTGGGnnnn_n'

Cuts at:   1197    4453    4539    4813    4894    5229    7373    1197 
   Size:       3256      86     274      81     335    2144    1425

  Fragments arranged by size: 

                3256    2144    1425     335     274      86      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3244    3407    5968    3244 
   Size:        163    2561    4877

  Fragments arranged by size: 

                4877    2561     163
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    196     221    2579    3118    4096    4159     196 
   Size:         25    2358     539     978      63    3638

  Fragments arranged by size: 

                3638    2358     978     539      63      25
 
Bpu10I CC'TnA_GC

Cuts at:    115     307     478    1420    1756    3554     115 
   Size:        192     171     942     336    1798    4162

  Fragments arranged by size: 

                4162    1798     942     336     192     171
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    636    1185    1318    3322    3855    4777    5656    6370    6668 
   Size:        549     133    2004     533     922     879     714     298

Cuts at:   6668     636 
   Size:       1569

  Fragments arranged by size: 

                2004    1569     922     879     714     549     533     298
                 133
 
BsaI GGTCTCn'nnnn_

Cuts at:    524    1150    1406    2624    3204    3395    3429    3757    7305 
   Size:        626     256    1218     580     191      34     328    3548

Cuts at:   7305     524 
   Size:        820

  Fragments arranged by size: 

                3548    1218     820     626     580     328     256     191
                  34
 
BsaAI yAC'GTr

Cuts at:    609    4954     609 
   Size:       4345    3256
 
BsaBI GATnn'nnATC

Cuts at:      6    5115    7152       6 
   Size:       5109    2037     455

  Fragments arranged by size: 

                5109    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    141    1571    2000    2105    3775    4081    5032    5280    5982 
   Size:       1430     429     105    1670     306     951     248     702

Cuts at:   5982    7478     141 
   Size:       1496     264

  Fragments arranged by size: 

                1670    1496    1430     951     702     429     306     264
                 248     105
 
BsaWI w'CCGG_w

Cuts at:   1697    1742    3451    3647    3861    4790    5311    5989    6400 
   Size:         45    1709     196     214     929     521     678     411

Cuts at:   6400    6547    6821    1697 
   Size:        147     274    2477

  Fragments arranged by size: 

                2477    1709     929     678     521     411     274     214
                 196     147      45
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    219     249    1025    1055    2604    2634    4046    4076     219 
   Size:         30     776      30    1549      30    1412      30    3744

  Fragments arranged by size: 

                3744    1549    1412     776      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    460    1051    1597    3049    3251    3483    3683    4125     460 
   Size:        591     546    1452     202     232     200     442    3936

  Fragments arranged by size: 

                3936    1452     591     546     442     232     202     200
            
 
BseYI C'CCAG_C

Cuts at:    135    1796    1836    3265    5026    5784    6449    6857     135 
   Size:       1661      40    1429    1761     758     665     408     879

  Fragments arranged by size: 

                1761    1661    1429     879     758     665     408      40
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    345    1168    1315     345 
   Size:        823     147    6631

  Fragments arranged by size: 

                6631     823     147
 
BsiEI CG_ry'CG

Cuts at:    104     189    2171    2690    5143    5189    6419     104 
   Size:         85    1982     519    2453      46    1230    1286

  Fragments arranged by size: 

                2453    1982    1286    1230     519      85      46
 
BsiHKAI G_wGCw'C

Cuts at:   1046    1757    2727    2852    3338    3552    4408    4615    5393 
   Size:        711     970     125     486     214     856     207     778

Cuts at:   5393    5583    6443    1046 
   Size:        190     860    2204

  Fragments arranged by size: 

                2204     970     860     856     778     711     486     214
                 207     190     125
 
BsiWI C'GTAC_G

Cuts at:    287     287 
   Size:       7601
 
BsmBI CGTCTCn'nnnn_

Cuts at:    335     729    3215     335 
   Size:        394    2486    4721

  Fragments arranged by size: 

                4721    2486     394
 
Bsp1286I G_dGCh'C

Cuts at:    614    1046    1449    1491    1757    2631    2727    2767    2852 
   Size:        432     403      42     266     874      96      40      85

Cuts at:   2852    3338    3552    3740    3908    4097    4217    4234    4408 
   Size:        486     214     188     168     189     120      17     174

Cuts at:   4408    4438    4615    5226    5319    5393    5583    5645    6443 
   Size:         30     177     611      93      74     190      62     798

Cuts at:   6443     614 
   Size:       1772

  Fragments arranged by size: 

                1772     874     798     611     486     432     403     266
                 214     190     189     188     177     174     168     120
                  96      93      85      74      62      42      40      30
                  17
 
BspEI T'CCGG_A

Cuts at:   1697    1742    1697 
   Size:         45    7556
 
BspHI T'CATG_A

Cuts at:   2259    4506    6033    2259 
   Size:       2247    1527    3827

  Fragments arranged by size: 

                3827    2247    1527
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    990    2846    3285    3377    4137    4151    4230    4560    4651 
   Size:       1856     439      92     760      14      79     330      91

Cuts at:   4651    4865    5167    5548     990 
   Size:        214     302     381    3043

  Fragments arranged by size: 

                3043    1856     760     439     381     330     302     214
                  92      91      79      14
 
BsrBI CCG'CTC

Cuts at:     50    2193    5893    5947    7431    7596      50 
   Size:       2143    3700      54    1484     165      55

  Fragments arranged by size: 

                3700    2143    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   5513    5513 
   Size:       7601
 
BsrFI r'CCGG_y

Cuts at:    214    3407    3451    5599    5780     214 
   Size:       3193      44    2148     181    2035

  Fragments arranged by size: 

                3193    2148    2035     181      44
 
BssHII G'CGCG_C

Cuts at:    181     181 
   Size:       7601
 
BssSI C'ACGA_G

Cuts at:   5872    6580    5872 
   Size:        708    6893
 
BstAPI GCAn_nnn'nTGC

Cuts at:    972    4152    4978     972 
   Size:       3180     826    3595

  Fragments arranged by size: 

                3595    3180     826
 
BstBI TT'CG_AA

Cuts at:   5962    5962 
   Size:       7601
 
BstEII G'GTnAC_C

Cuts at:    672     672 
   Size:       7601
 
BstXI CCAn_nnnn'nTGG

Cuts at:    769     804    3255     769 
   Size:         35    2451    5115

  Fragments arranged by size: 

                5115    2451      35
 
BstYI r'GATC_y

Cuts at:      1    1643    1694    4446    4626    5093    5116    5451    5697 
   Size:       1642      51    2752     180     467      23     335     246

Cuts at:   5697    6003    6015    6101    7153       1 
   Size:        306      12      86    1052     449

  Fragments arranged by size: 

                2752    1642    1052     467     449     335     306     246
                 180      86      51      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   4521    4521 
   Size:       7601
 
BtgI C'CryG_G

Cuts at:    107     768    3504    3698    4226     107 
   Size:        661    2736     194     528    3482

  Fragments arranged by size: 

                3482    2736     661     528     194
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1290    4304    4318    5551    5732    5895    1290 
   Size:       3014      14    1233     181     163    2996

  Fragments arranged by size: 

                3014    2996    1233     181     163      14
 
BtsI GCAGTG_nn'

Cuts at:    404    2993    3077    4203    4564    4844     404 
   Size:       2589      84    1126     361     280    3161

  Fragments arranged by size: 

                3161    2589    1126     361     280      84
 
DraI TTT'AAA

Cuts at:   4600    4600 
   Size:       7601
 
DraIII CAC_nnn'GTG

Cuts at:    630     972    4533     630 
   Size:        342    3561    3698

  Fragments arranged by size: 

                3698    3561     342
 
DrdI GACnn_nn'nnGTC

Cuts at:    535    2602    5307    6651     535 
   Size:       2067    2705    1344    1485

  Fragments arranged by size: 

                2705    2067    1485    1344
 
EaeI y'GGCC_r

Cuts at:    186     224     505     786    1410    2168    3415    5186    5360 
   Size:         38     281     281     624     758    1247    1771     174

Cuts at:   5360    5751    5778    7123     186 
   Size:        391      27    1345     664

  Fragments arranged by size: 

                1771    1345    1247     758     664     624     391     281
                 281     174      38      27
 
EagI C'GGCC_G

Cuts at:    186    2168    5186     186 
   Size:       1982    3018    2601

  Fragments arranged by size: 

                3018    2601    1982
 
EarI CTCTTCn'nnn_

Cuts at:    428     431    1859    2807    5624    5834     428 
   Size:          3    1428     948    2817     210    2195

  Fragments arranged by size: 

                2817    2195    1428     948     210       3
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1046    1780    3358    5809    6551    6697    7411    1046 
   Size:        734    1578    2451     742     146     714    1236

  Fragments arranged by size: 

                2451    1578    1236     742     734     714     146
 
EcoICRI GAG'CTC

Cuts at:   2850    3336    4406    4613    2850 
   Size:        486    1070     207    5838

  Fragments arranged by size: 

                5838    1070     486     207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    196     221     412     673     883    1000    1498    1924    2579 
   Size:         25     191     261     210     117     498     426     655

Cuts at:   2579    3118    3461    4096    4159    4460    5426    5858    6211 
   Size:        539     343     635      63     301     966     432     353

Cuts at:   6211    6873     196 
   Size:        662     924

  Fragments arranged by size: 

                 966     924     662     655     635     539     498     432
                 426     353     343     301     261     210     191     117
                  63      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1423    3837    4049    1423 
   Size:       2414     212    4975

  Fragments arranged by size: 

                4975    2414     212
 
EcoO109I rG'GnC_Cy

Cuts at:   1317    1712    2763    2764    2835    3711    3905    3927    4149 
   Size:        395    1051       1      71     876     194      22     222

Cuts at:   4149    4179    4344    4740    1317 
   Size:         30     165     396    4178

  Fragments arranged by size: 

                4178    1051     876     396     395     222     194     165
                  71      30      22       1
 
EcoRI G'AATT_C

Cuts at:    377    1675    3181    4605     377 
   Size:       1298    1506    1424    3373

  Fragments arranged by size: 

                3373    1506    1424    1298
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3608    3849    5620    3608 
   Size:        241    1771    5589

  Fragments arranged by size: 

                5589    1771     241
 
FspI TGC'GCA

Cuts at:   5382    5382 
   Size:       7601
 
HaeII r_GCGC'y

Cuts at:    144    1627    2108    3778    4084    4475    5035    5283    6513 
   Size:       1483     481    1670     306     391     560     248    1230

Cuts at:   6513    7284     144 
   Size:        771     461

  Fragments arranged by size: 

                1670    1483    1230     771     560     481     461     391
                 306     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    550     582     826     858    1248    1280    1650    1654    1682 
   Size:         32     244      32     390      32     370       4      28

Cuts at:   1682    1686    2446    2478    2920    2952    3023    3055    3118 
   Size:          4     760      32     442      32      71      32      63

Cuts at:   3118    3150    3214    3246    3933    3965    4192    4224    4447 
   Size:         32      64      32     687      32     227      32     223

Cuts at:   4447    4479    5443    5473    5475    5505     550 
   Size:         32     964      30       2      30    2646

  Fragments arranged by size: 

                2646     964     760     687     442     390     370     244
                 227     223      71      64      63      32      32      32
                  32      32      32      32      32      32      32      32
                  30      30      28       4       4       2
 
HincII GTy'rAC

Cuts at:   1368    1979    2595    4640    1368 
   Size:        611     616    2045    4329

  Fragments arranged by size: 

                4329    2045     616     611
 
KasI G'GCGC_C

Cuts at:    140    2104    3774    4080    5031    5279     140 
   Size:       1964    1670     306     951     248    2462

  Fragments arranged by size: 

                2462    1964    1670     951     306     248
 
KpnI G_GTAC'C

Cuts at:   4105    4621    4105 
   Size:        516    7085
 
MfeI C'AATT_G

Cuts at:   2879    2879 
   Size:       7601
 
MluI A'CGCG_T

Cuts at:    284    7034     284 
   Size:       6750     851
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    821    1541    3328    3472    6360    6544     821 
   Size:        720    1787     144    2888     184    1878

  Fragments arranged by size: 

                2888    1878    1787     720     184     144
 
MscI TGG'CCA

Cuts at:    226     507    1412    5362     226 
   Size:        281     905    3950    2465

  Fragments arranged by size: 

                3950    2465     905     281
 
MslI CAynn'nnrTG

Cuts at:    367     767    1099    2264    5590    5717     367 
   Size:        400     332    1165    3326     127    2251

  Fragments arranged by size: 

                3326    2251    1165     400     332     127
 
MspA1I CmG'CkG

Cuts at:    600     876    1840    1992    2557    4228    4468    4480    4712 
   Size:        276     964     152     565    1671     240      12     232

Cuts at:   4712    5026    5386    6170    6415     600 
   Size:        314     360     784     245    1786

  Fragments arranged by size: 

                1786    1671     964     784     565     360     314     276
                 245     240     232     152      12
 
NaeI GCC'GGC

Cuts at:    216    5782     216 
   Size:       5566    2035
 
NarI GG'CG_CC

Cuts at:    141    2105    3775    4081    5032    5280     141 
   Size:       1964    1670     306     951     248    2462

  Fragments arranged by size: 

                2462    1964    1670     951     306     248
 
NcoI C'CATG_G

Cuts at:    107    3504     107 
   Size:       3397    4204
 
NdeI CA'TA_TG

Cuts at:   2152    2152 
   Size:       7601
 
NgoMIV G'CCGG_C

Cuts at:    214    5780     214 
   Size:       5566    2035
 
NotI GC'GGCC_GC

Cuts at:    186     186 
   Size:       7601
 
NspI r_CATG'y

Cuts at:   1608    4654    5154    6757    1608 
   Size:       3046     500    1603    2452

  Fragments arranged by size: 

                3046    2452    1603     500
 
PasI CC'CwG_GG

Cuts at:   1341    1341 
   Size:       7601
 
PciI A'CATG_T

Cuts at:   6753    6753 
   Size:       7601
 
PflMI CCAn_nnn'nTGG

Cuts at:    514    3014    4330     514 
   Size:       2500    1316    3785

  Fragments arranged by size: 

                3785    2500    1316
 
PfoI T'CCnGG_A

Cuts at:   2741    2951    3656    2741 
   Size:        210     705    6686

  Fragments arranged by size: 

                6686     705     210
 
PmeI GTTT'AAAC

Cuts at:   4600    4600 
   Size:       7601
 
PmlI CAC'GTG

Cuts at:    609     609 
   Size:       7601
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    854     886    6047    6079     854 
   Size:         32    5161      32    2376

  Fragments arranged by size: 

                5161    2376      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1317    1712    2835    3711    4149    4344    1317 
   Size:        395    1123     876     438     195    4574

  Fragments arranged by size: 

                4574    1123     876     438     395     195
 
PsiI TTA'TAA

Cuts at:   3596    3596 
   Size:       7601
 
PspOMI G'GGCC_C

Cuts at:   2763    3904    2763 
   Size:       1141    6460
 
PspXI vC'TCGA_Gb

Cuts at:   1312    3950    1312 
   Size:       2638    4963
 
PstI C_TGCA'G

Cuts at:   1331    2636    4002    4148    4157    4571    4648    5333    1331 
   Size:       1305    1366     146       9     414      77     685    3599

  Fragments arranged by size: 

                3599    1366    1305     685     414     146      77       9
            
 
PvuI CG_AT'CG

Cuts at:    104    2690     104 
   Size:       2586    5015
 
PvuII CAG'CTG

Cuts at:    600    1840    1992    2557    4468    4480    5026    5386     600 
   Size:       1240     152     565    1911      12     546     360    2815

  Fragments arranged by size: 

                2815    1911    1240     565     546     360     152      12
            
 
RsrII CG'GwC_CG

Cuts at:   5796    5796 
   Size:       7601
 
SacI G_AGCT'C

Cuts at:   2852    3338    4408    4615    2852 
   Size:        486    1070     207    5838

  Fragments arranged by size: 

                5838    1070     486     207
 
SacII CC_GC'GG

Cuts at:   4229    4229 
   Size:       7601
 
SalI G'TCGA_C

Cuts at:   4638    4638 
   Size:       7601
 
SapI GCTCTTCn'nnn_

Cuts at:   5624    5834    5624 
   Size:        210    7391
 
SbfI CC_TGCA'GG

Cuts at:   4148    4648    4148 
   Size:        500    7101
 
ScaI AGT'ACT

Cuts at:   1908    7118    1908 
   Size:       5210    2391
 
SexAI A'CCwGG_T

Cuts at:    626    2614    3326     626 
   Size:       1988     712    4901

  Fragments arranged by size: 

                4901    1988     712
 
SfcI C'TryA_G

Cuts at:     33     162    1327    2374    2380    2632    3998    4144    4153 
   Size:        129    1165    1047       6     252    1366     146       9

Cuts at:   4153    4567    4644    5329    6297    6488      33 
   Size:        414      77     685     968     191    1146

  Fragments arranged by size: 

                1366    1165    1146    1047     968     685     414     252
                 191     146     129      77       9       6
 
SfoI GGC'GCC

Cuts at:    142    2106    3776    4082    5033    5281     142 
   Size:       1964    1670     306     951     248    2462

  Fragments arranged by size: 

                2462    1964    1670     951     306     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7601
 
SmaI CCC'GGG

Cuts at:   4623    4623 
   Size:       7601
 
SmlI C'TyrA_G

Cuts at:    615    1164    1312    1333    3337    3834    3950    4756    5671 
   Size:        549     148      21    2004     497     116     806     915

Cuts at:   5671    6385    6647     615 
   Size:        714     262    1569

  Fragments arranged by size: 

                2004    1569     915     806     714     549     497     262
                 148     116      21
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7601
 
SphI G_CATG'C

Cuts at:   1608    4654    5154    1608 
   Size:       3046     500    4055

  Fragments arranged by size: 

                4055    3046     500
 
SspI AAT'ATT

Cuts at:   1893    1893 
   Size:       7601
 
StuI AGG'CCT

Cuts at:    715     935     715 
   Size:        220    7381
 
StyI C'CwwG_G

Cuts at:    107     440     710    1463    2861    2901    3008    3504    3566 
   Size:        333     270     753    1398      40     107     496      62

Cuts at:   3566    3706    4097    4484    4735    5089     107 
   Size:        140     391     387     251     354    2619

  Fragments arranged by size: 

                2619    1398     753     496     391     387     354     333
                 270     251     140     107      62      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1295    2233    2710    3015    3387    3517    3819    4232    4521 
   Size:        938     477     305     372     130     302     413     289

Cuts at:   4521    5184    5317    5776    5985    1295 
   Size:        663     133     459     209    2911

  Fragments arranged by size: 

                2911     938     663     477     459     413     372     305
                 302     289     209     133     130
 
TatI w'GTAC_w

Cuts at:   1906    7116    1906 
   Size:       5210    2391
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    219     251    1025    1057    2604    2636    4046    4078    7118 
   Size:         32     774      32    1547      32    1410      32    3040

Cuts at:   7118    7150     219 
   Size:         32     670

  Fragments arranged by size: 

                3040    1547    1410     774     670      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   5398    5398 
   Size:       7601
 
XbaI T'CTAG_A

Cuts at:   2574    2586    4632    2574 
   Size:         12    2046    5543

  Fragments arranged by size: 

                5543    2046      12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   5096    5096 
   Size:       7601
 
XhoI C'TCGA_G

Cuts at:   1312    3950    1312 
   Size:       2638    4963
 
XmaI C'CCGG_G

Cuts at:   4621    4621 
   Size:       7601
 
XmnI GAAnn'nnTTC

Cuts at:    583    1937     583 
   Size:       1354    6247
 
ZraI GAC'GTC

Cuts at:   1572    1572 
   Size:       7601
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AloI     AscI    AvrII     BaeI    BfrBI     BmtI
     BsmI    BsrGI  BstZ17I     ClaI    CspCI    EcoRV     FseI    FspAI
  HindIII     HpaI     NheI     NruI     NsiI     PacI    PshAI     PsrI
    SanDI     SfiI    SgrAI    SnaBI     SrfI     SwaI
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