Restriction map for pF1KA0305
 (Circular) (Six-base) MAPSORT of: pF1KA0305.seq  Check: 9800  from: 1  to: 7730



pF1KA0305  7730 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AccI GT'mk_AC

Cuts at:   2634    3817    3990    4235    4768    2634 
   Size:       1183     173     245     533    5596

  Fragments arranged by size: 

                5596    1183     533     245     173
 
Acc65I G'GTAC_C

Cuts at:   4746    4746 
   Size:       7730
 
AclI AA'CG_TT

Cuts at:   7570    7570 
   Size:       7730
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    224     422     593    1175    1423    1717    2264    2816    3053 
   Size:        198     171     582     248     294     547     552     237

Cuts at:   3053    3435    4082    4295    4610    5555    5987    6340    7002 
   Size:        382     647     213     315     945     432     353     662

Cuts at:   7002     224 
   Size:        952

  Fragments arranged by size: 

                 952     945     662     647     582     552     547     432
                 382     353     315     294     248     237     213     198
                 171
 
AflII C'TTAA_G

Cuts at:   1159    2569    1159 
   Size:       1410    6320
 
AflIII A'CryG_T

Cuts at:    836    1200    4213    5082    6882    7163     836 
   Size:        364    3013     869    1800     281    1403

  Fragments arranged by size: 

                3013    1800    1403     869     364     281
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2611    2643    2611 
   Size:         32    7698
 
AlwNI CAG_nnn'CTG

Cuts at:   2017    3031    3214    4588    5035    6473    2017 
   Size:       1014     183    1374     447    1438    3274

  Fragments arranged by size: 

                3274    1438    1374    1014     447     183
 
ApaLI G'TGCA_C

Cuts at:   6568    6568 
   Size:       7730
 
ApoI r'AATT_y

Cuts at:    783    1059    1074    2383    3322    3632    3918    4004    4573 
   Size:        276      15    1309     939     310     286      86     569

Cuts at:   4573    4734    7083    7339     783 
   Size:        161    2349     256    1174

  Fragments arranged by size: 

                2349    1309    1174     939     569     310     286     276
                 256     161      86      15
 
AseI AT'TA_AT

Cuts at:     20    1270    1447    1607    1883      20 
   Size:       1250     177     160     276    5867

  Fragments arranged by size: 

                5867    1250     276     177     160
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7730
 
AvaI C'yCGr_G

Cuts at:   4750    4750 
   Size:       7730
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2144    2177    2144 
   Size:         33    7697
 
BamHI G'GATC_C

Cuts at:   4755    4755 
   Size:       7730
 
BanI G'GyrC_C

Cuts at:   4746    5160    5408    5443    4746 
   Size:        414     248      35    7033

  Fragments arranged by size: 

                7033     414     248      35
 
BanII G_rGCy'C

Cuts at:   4086    4744    5774    4086 
   Size:        658    1030    6042

  Fragments arranged by size: 

                6042    1030     658
 
BbeI G_GCGC'C

Cuts at:   5164    5412    5164 
   Size:        248    7482
 
BbsI GAAGACnn'nnnn_

Cuts at:   1145    1231    1469    1574    2804    3139    1145 
   Size:         86     238     105    1230     335    5736

  Fragments arranged by size: 

                5736    1230     335     238     105      86
 
BbvCI CC'TCA_GC

Cuts at:   1304    4607    1304 
   Size:       3303    4427
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2393    2427    2393 
   Size:         34    7696
 
BciVI GTATCCnnnnn_n'

Cuts at:   5628    6684    7730    5628 
   Size:       1056    1046    5628

  Fragments arranged by size: 

                5628    1056    1046
 
BclI T'GATC_A

Cuts at:   5250    5250 
   Size:       7730
 
BfrBI ATG'CAT

Cuts at:    211     211 
   Size:       7730
 
BglII A'GATC_T

Cuts at:    800    5245    7282     800 
   Size:       4445    2037    1248

  Fragments arranged by size: 

                4445    2037    1248
 
BlpI GC'TnA_GC

Cuts at:   4842    4842 
   Size:       7730
 
Bme1580I G_kGCm'C

Cuts at:   4498    5355    5448    6572    4498 
   Size:        857      93    1124    5656

  Fragments arranged by size: 

                5656    1124     857      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   4942    5023    5358    7502    4942 
   Size:         81     335    2144    5170

  Fragments arranged by size: 

                5170    2144     335      81
 
BmtI G_CTAG'C

Cuts at:   4183    4183 
   Size:       7730
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6097    6097 
   Size:       7730
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3215    4139    3215 
   Size:        924    6806
 
Bpu10I CC'TnA_GC

Cuts at:   1304    2054    2528    4607    1304 
   Size:        750     474    2079    4427

  Fragments arranged by size: 

                4427    2079     750     474
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    346    1532    3670    4906    5785    6499    6797     346 
   Size:       1186    2138    1236     879     714     298    1279

  Fragments arranged by size: 

                2138    1279    1236    1186     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4153    7434    4153 
   Size:       3281    4449
 
BsaAI yAC'GTr

Cuts at:   4251    5083    4251 
   Size:        832    6898
 
BsaBI GATnn'nnATC

Cuts at:      6    3977    5244    7281       6 
   Size:       3971    1267    2037     455

  Fragments arranged by size: 

                3971    2037    1267     455
 
BsaHI Gr'CG_yC

Cuts at:   5161    5409    6111    7607    5161 
   Size:        248     702    1496    5284

  Fragments arranged by size: 

                5284    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   4919    5440    6118    6529    6676    6950    4919 
   Size:        521     678     411     147     274    5699

  Fragments arranged by size: 

                5699     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   2600    3716    2600 
   Size:       1116    6614
 
BseYI C'CCAG_C

Cuts at:   2140    4262    5155    5913    6578    6986    2140 
   Size:       2122     893     758     665     408    2884

  Fragments arranged by size: 

                2884    2122     893     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1115    3722    1115 
   Size:       2607    5123
 
BsiEI CG_ry'CG

Cuts at:    104    5272    5318    6548     104 
   Size:       5168      46    1230    1286

  Fragments arranged by size: 

                5168    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   4086    4638    4744    5522    5712    6572    4086 
   Size:        552     106     778     190     860    5244

  Fragments arranged by size: 

                5244     860     778     552     190     106
 
Bsp1286I G_dGCh'C

Cuts at:   4086    4498    4638    4744    5355    5448    5522    5712    5774 
   Size:        412     140     106     611      93      74     190      62

Cuts at:   5774    6572    4086 
   Size:        798    5244

  Fragments arranged by size: 

                5244     798     611     412     190     140     106      93
                  74      62
 
BspHI T'CATG_A

Cuts at:   3237    6162    3237 
   Size:       2925    4805
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1024    4780    4994    5296    5677    1024 
   Size:       3756     214     302     381    3077

  Fragments arranged by size: 

                3756    3077     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    6022    6076    7560    7725      50 
   Size:       5972      54    1484     165      55

  Fragments arranged by size: 

                5972    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    268    1629    2409    5642     268 
   Size:       1361     780    3233    2356

  Fragments arranged by size: 

                3233    2356    1361     780
 
BsrFI r'CCGG_y

Cuts at:   5728    5909    5728 
   Size:        181    7549
 
BsrGI T'GTAC_A

Cuts at:    201     201 
   Size:       7730
 
BssSI C'ACGA_G

Cuts at:   3310    6001    6709    3310 
   Size:       2691     708    4331

  Fragments arranged by size: 

                4331    2691     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3721    5107    3721 
   Size:       1386    6344
 
BstBI TT'CG_AA

Cuts at:   3804    6091    3804 
   Size:       2287    5443
 
BstEII G'GTnAC_C

Cuts at:   1973    2981    1973 
   Size:       1008    6722
 
BstXI CCAn_nnnn'nTGG

Cuts at:    913     913 
   Size:       7730
 
BstYI r'GATC_y

Cuts at:      1     800    1476    2251    4585    4755    5222    5245    5580 
   Size:        799     676     775    2334     170     467      23     335

Cuts at:   5580    5826    6132    6144    6230    7282       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                2334    1052     799     775     676     467     449     335
                 306     246     170      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   2635    3818    3991    2635 
   Size:       1183     173    6374

  Fragments arranged by size: 

                6374    1183     173
 
Bsu36I CC'TnA_GG

Cuts at:   2612    3440    2612 
   Size:        828    6902
 
BtgI C'CryG_G

Cuts at:    107    3883    4472    4653     107 
   Size:       3776     589     181    3184

  Fragments arranged by size: 

                3776    3184     589     181
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   5680    5861    6024    5680 
   Size:        181     163    7386

  Fragments arranged by size: 

                7386     181     163
 
BtsI GCAGTG_nn'

Cuts at:    225    1343    4973     225 
   Size:       1118    3630    2982

  Fragments arranged by size: 

                3630    2982    1118
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   4049    4084    4049 
   Size:         35    7695
 
DraI TTT'AAA

Cuts at:    123     788     986    4206    4729     123 
   Size:        665     198    3220     523    3124

  Fragments arranged by size: 

                3220    3124     665     523     198
 
DraIII CAC_nnn'GTG

Cuts at:   1291    1291 
   Size:       7730
 
DrdI GACnn_nn'nnGTC

Cuts at:   5436    6780    5436 
   Size:       1344    6386
 
EaeI y'GGCC_r

Cuts at:   4035    5315    5489    5880    5907    7252    4035 
   Size:       1280     174     391      27    1345    4513

  Fragments arranged by size: 

                4513    1345    1280     391     174      27
 
EagI C'GGCC_G

Cuts at:   5315    5315 
   Size:       7730
 
EarI CTCTTCn'nnn_

Cuts at:    986    1194    2704    5753    5963     986 
   Size:        208    1510    3049     210    2753

  Fragments arranged by size: 

                3049    2753    1510     210     208
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   5938    6680    6826    7540    5938 
   Size:        742     146     714    6128

  Fragments arranged by size: 

                6128     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   4084    4742    4084 
   Size:        658    7072
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    224     422     593    1175    1423    1717    2264    2816    3053 
   Size:        198     171     582     248     294     547     552     237

Cuts at:   3053    3215    3435    4082    4139    4295    4610    5555    5987 
   Size:        162     220     647      57     156     315     945     432

Cuts at:   5987    6340    7002     224 
   Size:        353     662     952

  Fragments arranged by size: 

                 952     945     662     647     582     552     547     432
                 353     315     294     248     237     220     198     171
                 162     156      57
 
EcoO109I rG'GnC_Cy

Cuts at:   2097    4661    4869    2097 
   Size:       2564     208    4958

  Fragments arranged by size: 

                4958    2564     208
 
EcoRI G'AATT_C

Cuts at:   3632    4734    3632 
   Size:       1102    6628
 
EcoRV GAT'ATC

Cuts at:   4257    4257 
   Size:       7730
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1573    3465    3635    4615    5749    1573 
   Size:       1892     170     980    1134    3554

  Fragments arranged by size: 

                3554    1892    1134     980     170
 
FspI TGC'GCA

Cuts at:   5511    5511 
   Size:       7730
 
HaeII r_GCGC'y

Cuts at:   5164    5412    6642    7413    5164 
   Size:        248    1230     771    5481

  Fragments arranged by size: 

                5481    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    556     588     792     824    1207    1239    1765    1797    1986 
   Size:         32     204      32     383      32     526      32     189

Cuts at:   1986    2018    2535    2567    3446    3478    3586    3618    4303 
   Size:         32     517      32     879      32     108      32     685

Cuts at:   4303    4335    5572    5602    5604    5634     556 
   Size:         32    1237      30       2      30    2652

  Fragments arranged by size: 

                2652    1237     879     685     526     517     383     204
                 189     108      32      32      32      32      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   3066    3798    4769    3066 
   Size:        732     971    6027

  Fragments arranged by size: 

                6027     971     732
 
HindIII A'AGCT_T

Cuts at:   3646    3646 
   Size:       7730
 
HpaI GTT'AAC

Cuts at:   3066    3066 
   Size:       7730
 
KasI G'GCGC_C

Cuts at:   5160    5408    5160 
   Size:        248    7482
 
KpnI G_GTAC'C

Cuts at:   4750    4750 
   Size:       7730
 
MfeI C'AATT_G

Cuts at:    821    2059     821 
   Size:       1238    6492
 
MluI A'CGCG_T

Cuts at:   7163    7163 
   Size:       7730
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2019    2578    4691    6489    6673    2019 
   Size:        559    2113    1798     184    3076

  Fragments arranged by size: 

                3076    2113    1798     559     184
 
MscI TGG'CCA

Cuts at:   4037    5491    4037 
   Size:       1454    6276
 
MslI CAynn'nnrTG

Cuts at:    650    2585    3781    4564    5719    5846     650 
   Size:       1935    1196     783    1155     127    2534

  Fragments arranged by size: 

                2534    1935    1196    1155     783     127
 
MspA1I CmG'CkG

Cuts at:    130    2191    4841    5155    5515    6299    6544     130 
   Size:       2061    2650     314     360     784     245    1316

  Fragments arranged by size: 

                2650    2061    1316     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   5911    5911 
   Size:       7730
 
NarI GG'CG_CC

Cuts at:   5161    5409    5161 
   Size:        248    7482
 
NcoI C'CATG_G

Cuts at:    107    4472    4653     107 
   Size:       4365     181    3184

  Fragments arranged by size: 

                4365    3184     181
 
NdeI CA'TA_TG

Cuts at:    213    3851     213 
   Size:       3638    4092
 
NgoMIV G'CCGG_C

Cuts at:   5909    5909 
   Size:       7730
 
NheI G'CTAG_C

Cuts at:   4179    4179 
   Size:       7730
 
NsiI A_TGCA'T

Cuts at:    213     213 
   Size:       7730
 
NspI r_CATG'y

Cuts at:    840    1204    2423    3937    4217    4783    5283    6886     840 
   Size:        364    1219    1514     280     566     500    1603    1684

  Fragments arranged by size: 

                1684    1603    1514    1219     566     500     364     280
            
 
PasI CC'CwG_GG

Cuts at:   3659    3659 
   Size:       7730
 
PciI A'CATG_T

Cuts at:    836    1200    4213    6882     836 
   Size:        364    3013    2669    1684

  Fragments arranged by size: 

                3013    2669    1684     364
 
PmeI GTTT'AAAC

Cuts at:   4729    4729 
   Size:       7730
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6176    6208    6176 
   Size:         32    7698
 
PpuMI rG'GwC_Cy

Cuts at:   2097    4661    2097 
   Size:       2564    5166
 
PshAI GACnn'nnGTC

Cuts at:   2804    2804 
   Size:       7730
 
PsiI TTA'TAA

Cuts at:   1120    1120 
   Size:       7730
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2775    2807    2775 
   Size:         32    7698
 
PstI C_TGCA'G

Cuts at:   1021    4777    5462    1021 
   Size:       3756     685    3289

  Fragments arranged by size: 

                3756    3289     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7730
 
PvuII CAG'CTG

Cuts at:    130    2191    5155    5515     130 
   Size:       2061    2964     360    2345

  Fragments arranged by size: 

                2964    2345    2061     360
 
RsrII CG'GwC_CG

Cuts at:   5925    5925 
   Size:       7730
 
SacI G_AGCT'C

Cuts at:   4086    4744    4086 
   Size:        658    7072
 
SalI G'TCGA_C

Cuts at:   4767    4767 
   Size:       7730
 
SapI GCTCTTCn'nnn_

Cuts at:   5753    5963    5753 
   Size:        210    7520
 
SbfI CC_TGCA'GG

Cuts at:   4777    4777 
   Size:       7730
 
ScaI AGT'ACT

Cuts at:   3914    7247    3914 
   Size:       3333    4397
 
SexAI A'CCwGG_T

Cuts at:   2676    2676 
   Size:       7730
 
SfcI C'TryA_G

Cuts at:     33    1017    1532    2177    2186    2864    3215    4773    5458 
   Size:        984     515     645       9     678     351    1558     685

Cuts at:   5458    6426    6617      33 
   Size:        968     191    1146

  Fragments arranged by size: 

                1558    1146     984     968     685     678     645     515
                 351     191       9
 
SfoI GGC'GCC

Cuts at:   5162    5410    5162 
   Size:        248    7482
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7730
 
SmaI CCC'GGG

Cuts at:   4752    4752 
   Size:       7730
 
SmlI C'TyrA_G

Cuts at:    361    1159    1547    2569    3649    4885    5800    6514    6776 
   Size:        798     388    1022    1080    1236     915     714     262

Cuts at:   6776     361 
   Size:       1315

  Fragments arranged by size: 

                1315    1236    1080    1022     915     798     714     388
                 262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7730
 
SphI G_CATG'C

Cuts at:   4783    5283    4783 
   Size:        500    7230
 
SspI AAT'ATT

Cuts at:   1659    3349    3516    1659 
   Size:       1690     167    5873

  Fragments arranged by size: 

                5873    1690     167
 
StyI C'CwwG_G

Cuts at:    107    4472    4653    4864    5218     107 
   Size:       4365     181     211     354    2619

  Fragments arranged by size: 

                4365    2619     354     211     181
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   3688    5313    5446    5905    6114    3688 
   Size:       1625     133     459     209    5304

  Fragments arranged by size: 

                5304    1625     459     209     133
 
TatI w'GTAC_w

Cuts at:    201    2593    3893    3912    3961    7245     201 
   Size:       2392    1300      19      49    3284     686

  Fragments arranged by size: 

                3284    2392    1300     686      49      19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4023    4055    7247    7279    4023 
   Size:         32    3192      32    4474

  Fragments arranged by size: 

                4474    3192      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   5527    5527 
   Size:       7730
 
XbaI T'CTAG_A

Cuts at:   2475    4761    2475 
   Size:       2286    5444
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   5225    5225 
   Size:       7730
 
XmaI C'CCGG_G

Cuts at:   4750    4750 
   Size:       7730
 
XmnI GAAnn'nnTTC

Cuts at:   3272    3272 
   Size:       7730
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NsiI
     NspI     PasI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AgeI     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BglI    BmgBI    BsaXI    BsiWI     BsmI    BsmBI
    BspEI   BssHII     ClaI    EcoNI     FseI    FspAI     NotI     NruI
     PacI    PflMI     PfoI     PmlI   PspOMI    PspXI    SacII    SanDI
     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI     ZraI
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