Restriction map for pF1KA0144
 (Linear) (Six-base) MAPSORT of: KIAA0144.seq  Check: 4555  from: 1  to: 2949



KIAA0144.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1326    2949 
   Size:       1326    1623
 
AccI GT'mk_AC

Cuts at:      0    1648    1732    2949 
   Size:       1648      84    1217

  Fragments arranged by size: 

                1648    1217      84
 
Acc65I G'GTAC_C

Cuts at:      0    1802    2949 
   Size:       1802    1147
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     758     779     968    1288    1394    1845    2333    2461 
   Size:        758      21     189     320     106     451     488     128

Cuts at:   2461    2949 
   Size:        488

  Fragments arranged by size: 

                 758     488     488     451     320     189     128     106
                  21
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1419    2949 
   Size:       1419    1530
 
AleI CACnn'nnGTG

Cuts at:      0    2162    2164    2949 
   Size:       2162       2     785

  Fragments arranged by size: 

                2162     785       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     939    1452    1579    1921    2230    2949 
   Size:        939     513     127     342     309     719

  Fragments arranged by size: 

                 939     719     513     342     309     127
 
ApaI G_GGCC'C

Cuts at:      0    1768    2949 
   Size:       1768    1181
 
ApaLI G'TGCA_C

Cuts at:      0    1240    2949 
   Size:       1240    1709
 
AvaI C'yCGr_G

Cuts at:      0      29    2751    2949 
   Size:         29    2722     198

  Fragments arranged by size: 

                2722     198      29
 
AvrII C'CTAG_G

Cuts at:      0    2295    2949 
   Size:       2295     654
 
BamHI G'GATC_C

Cuts at:      0     906    2949 
   Size:        906    2043
 
BanI G'GyrC_C

Cuts at:      0     427     475    1802    2949 
   Size:        427      48    1327    1147

  Fragments arranged by size: 

                1327    1147     427      48
 
BanII G_rGCy'C

Cuts at:      0      30    1180    1768    2949 
   Size:         30    1150     588    1181

  Fragments arranged by size: 

                1181    1150     588      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     785     874    1349    2949 
   Size:        785      89     475    1600

  Fragments arranged by size: 

                1600     785     475      89
 
BbvCI CC'TCA_GC

Cuts at:      0    1433    2290    2949 
   Size:       1433     857     659

  Fragments arranged by size: 

                1433     857     659
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2884    2949 
   Size:       2884      65
 
BfrBI ATG'CAT

Cuts at:      0    2845    2949 
   Size:       2845     104
 
BglII A'GATC_T

Cuts at:      0     753     770    2949 
   Size:        753      17    2179

  Fragments arranged by size: 

                2179     753      17
 
BlpI GC'TnA_GC

Cuts at:      0    1130    1442    1521    2949 
   Size:       1130     312      79    1428

  Fragments arranged by size: 

                1428    1130     312      79
 
Bme1580I G_kGCm'C

Cuts at:      0      19    1244    1768    2949 
   Size:         19    1225     524    1181

  Fragments arranged by size: 

                1225    1181     524      19
 
BmgBI CAC'GTC

Cuts at:      0     400    2949 
   Size:        400    2549
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      48    1074    2236    2549    2949 
   Size:         48    1026    1162     313     400

  Fragments arranged by size: 

                1162    1026     400     313      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2181    2244    2402    2949 
   Size:       2181      63     158     547

  Fragments arranged by size: 

                2181     547     158      63
 
Bpu10I CC'TnA_GC

Cuts at:      0    1433    1760    2290    2949 
   Size:       1433     327     530     659

  Fragments arranged by size: 

                1433     659     530     327
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     563    2055    2949 
   Size:        563    1492     894

  Fragments arranged by size: 

                1492     894     563
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     365     757    2888    2949 
   Size:        365     392    2131      61

  Fragments arranged by size: 

                2131     392     365      61
 
BsaAI yAC'GTr

Cuts at:      0     662    2949 
   Size:        662    2287
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1371    1401    1795    1825    2535    2565    2949 
   Size:       1371      30     394      30     710      30     384

  Fragments arranged by size: 

                1371     710     394     384      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     166     575     739    1152    1352    2062    2247    2351 
   Size:        166     409     164     413     200     710     185     104

Cuts at:   2351    2601    2949 
   Size:        250     348

  Fragments arranged by size: 

                 710     413     409     348     250     200     185     166
                 164     104
 
BseYI C'CCAG_C

Cuts at:      0     600     861     932    1807    2616    2761    2949 
   Size:        600     261      71     875     809     145     188

  Fragments arranged by size: 

                 875     809     600     261     188     145      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1225    1325    1891    2216    2949 
   Size:       1225     100     566     325     733

  Fragments arranged by size: 

                1225     733     566     325     100
 
BsiEI CG_ry'CG

Cuts at:      0    2598    2949 
   Size:       2598     351
 
BsiHKAI G_wGCw'C

Cuts at:      0    1244    2949 
   Size:       1244    1705
 
BsmI GAATG_Cn'

Cuts at:      0     279    1134    2013    2847    2949 
   Size:        279     855     879     834     102

  Fragments arranged by size: 

                 879     855     834     279     102
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     917    2949 
   Size:        917    2032
 
Bsp1286I G_dGCh'C

Cuts at:      0      19      30    1180    1244    1768    2949 
   Size:         19      11    1150      64     524    1181

  Fragments arranged by size: 

                1181    1150     524      64      19      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1326    1553    2949 
   Size:       1326     227    1396

  Fragments arranged by size: 

                1396    1326     227
 
BsrBI CCG'CTC

Cuts at:      0    1718    2949 
   Size:       1718    1231
 
BsrDI GCAATG_nn'

Cuts at:      0     231    1558    2949 
   Size:        231    1327    1391

  Fragments arranged by size: 

                1391    1327     231
 
BsrFI r'CCGG_y

Cuts at:      0    1437    2949 
   Size:       1437    1512
 
BssSI C'ACGA_G

Cuts at:      0    1611    2949 
   Size:       1611    1338
 
BstBI TT'CG_AA

Cuts at:      0     955    2949 
   Size:        955    1994
 
BstEII G'GTnAC_C

Cuts at:      0    2578    2949 
   Size:       2578     371
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     328    2634    2949 
   Size:        328    2306     315

  Fragments arranged by size: 

                2306     328     315
 
BstYI r'GATC_y

Cuts at:      0     753     770     906    1405    1783    2949 
   Size:        753      17     136     499     378    1166

  Fragments arranged by size: 

                1166     753     499     378     136      17
 
BstZ17I GTA'TAC

Cuts at:      0    1649    2949 
   Size:       1649    1300
 
BtgI C'CryG_G

Cuts at:      0     435     528    2598    2949 
   Size:        435      93    2070     351

  Fragments arranged by size: 

                2070     435     351      93
 
BtsI GCAGTG_nn'

Cuts at:      0      92     776     827    1244    1328    1931    2949 
   Size:         92     684      51     417      84     603    1018

  Fragments arranged by size: 

                1018     684     603     417      92      84      51
 
EaeI y'GGCC_r

Cuts at:      0      82     333     531     675    1658    2558    2595    2624 
   Size:         82     251     198     144     983     900      37      29

Cuts at:   2624    2849    2949 
   Size:        225     100

  Fragments arranged by size: 

                 983     900     251     225     198     144     100      82
                  37      29
 
EagI C'GGCC_G

Cuts at:      0    2595    2949 
   Size:       2595     354
 
EarI CTCTTCn'nnn_

Cuts at:      0     495    1626    1824    2814    2949 
   Size:        495    1131     198     990     135

  Fragments arranged by size: 

                1131     990     495     198     135
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     758     779     968    1288    1394    1845    2181    2244 
   Size:        758      21     189     320     106     451     336      63

Cuts at:   2244    2333    2402    2461    2949 
   Size:         89      69      59     488

  Fragments arranged by size: 

                 758     488     451     336     320     189     106      89
                  69      63      59      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     983    1843    2949 
   Size:        983     860    1106

  Fragments arranged by size: 

                1106     983     860
 
EcoO109I rG'GnC_Cy

Cuts at:      0     490    1764    1765    1836    2382    2748    2949 
   Size:        490    1274       1      71     546     366     201

  Fragments arranged by size: 

                1274     546     490     366     201      71       1
 
EcoRV GAT'ATC

Cuts at:      0    1530    2949 
   Size:       1530    1419
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1875    2949 
   Size:       1875    1074
 
HaeII r_GCGC'y

Cuts at:      0    1263    1619    2704    2949 
   Size:       1263     356    1085     245

  Fragments arranged by size: 

                1263    1085     356     245
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1162    1194    1405    1437    1482    1514    1582    1614 
   Size:       1162      32     211      32      45      32      68      32

Cuts at:   1614    1992    2024    2133    2165    2596    2628    2649    2681 
   Size:        378      32     109      32     431      32      21      32

Cuts at:   2681    2949 
   Size:        268

  Fragments arranged by size: 

                1162     431     378     268     211     109      68      45
                  32      32      32      32      32      32      32      32
                  21
 
HincII GTy'rAC

Cuts at:      0     237    1733    2949 
   Size:        237    1496    1216

  Fragments arranged by size: 

                1496    1216     237
 
KpnI G_GTAC'C

Cuts at:      0    1806    2949 
   Size:       1806    1143
 
MfeI C'AATT_G

Cuts at:      0     166    2949 
   Size:        166    2783
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2886    2949 
   Size:       2886      63
 
MscI TGG'CCA

Cuts at:      0     335     677    2560    2949 
   Size:        335     342    1883     389

  Fragments arranged by size: 

                1883     389     342     335
 
MslI CAynn'nnrTG

Cuts at:      0     137    2162    2164    2949 
   Size:        137    2025       2     785

  Fragments arranged by size: 

                2025     785     137       2
 
MspA1I CmG'CkG

Cuts at:      0      81     604     653    1339    2535    2949 
   Size:         81     523      49     686    1196     414

  Fragments arranged by size: 

                1196     686     523     414      81      49
 
NaeI GCC'GGC

Cuts at:      0    1439    2949 
   Size:       1439    1510
 
NgoMIV G'CCGG_C

Cuts at:      0    1437    2949 
   Size:       1437    1512
 
NsiI A_TGCA'T

Cuts at:      0    2847    2949 
   Size:       2847     102
 
NspI r_CATG'y

Cuts at:      0    1023    2434    2949 
   Size:       1023    1411     515

  Fragments arranged by size: 

                1411    1023     515
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     694     939    1086    1113    2949 
   Size:        694     245     147      27    1836

  Fragments arranged by size: 

                1836     694     245     147      27
 
PpuMI rG'GwC_Cy

Cuts at:      0    1836    2382    2748    2949 
   Size:       1836     546     366     201

  Fragments arranged by size: 

                1836     546     366     201
 
PshAI GACnn'nnGTC

Cuts at:      0     389    2949 
   Size:        389    2560
 
PspOMI G'GGCC_C

Cuts at:      0    1764    2949 
   Size:       1764    1185
 
PstI C_TGCA'G

Cuts at:      0      99    1323    1550    1926    1986    2949 
   Size:         99    1224     227     376      60     963

  Fragments arranged by size: 

                1224     963     376     227      99      60
 
PvuII CAG'CTG

Cuts at:      0     604    1339    2949 
   Size:        604     735    1610

  Fragments arranged by size: 

                1610     735     604
 
SalI G'TCGA_C

Cuts at:      0    1731    2949 
   Size:       1731    1218
 
SanDI GG'GwC_CC

Cuts at:      0    2382    2748    2949 
   Size:       2382     366     201

  Fragments arranged by size: 

                2382     366     201
 
ScaI AGT'ACT

Cuts at:      0    1068    2949 
   Size:       1068    1881
 
SfcI C'TryA_G

Cuts at:      0      95     387    1070    1319    1546    1913    1922    1982 
   Size:         95     292     683     249     227     367       9      60

Cuts at:   1982    2502    2715    2949 
   Size:        520     213     234

  Fragments arranged by size: 

                 683     520     367     292     249     234     227     213
                  95      60       9
 
SmaI CCC'GGG

Cuts at:      0      31    2753    2949 
   Size:         31    2722     196

  Fragments arranged by size: 

                2722     196      31
 
SmlI C'TyrA_G

Cuts at:      0     578    2034    2949 
   Size:        578    1456     915

  Fragments arranged by size: 

                1456     915     578
 
StyI C'CwwG_G

Cuts at:      0    2024    2295    2633    2949 
   Size:       2024     271     338     316

  Fragments arranged by size: 

                2024     338     316     271
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2017    2538    2949 
   Size:       2017     521     411

  Fragments arranged by size: 

                2017     521     411
 
TatI w'GTAC_w

Cuts at:      0    1066    2949 
   Size:       1066    1883
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1371    1403    2949 
   Size:       1371      32    1546

  Fragments arranged by size: 

                1546    1371      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     328    2869    2949 
   Size:        328    2541      80

  Fragments arranged by size: 

                2541     328      80
 
XmaI C'CCGG_G

Cuts at:      0      29    2751    2949 
   Size:         29    2722     198

  Fragments arranged by size: 

                2722     198      29
 
XmnI GAAnn'nnTTC

Cuts at:      0     570    1858    2949 
   Size:        570    1288    1091

  Fragments arranged by size: 

                1288    1091     570
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AhdI     AleI    AlwNI     ApaI
    ApaLI     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI
    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
     BtsI     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI    HaeII    Hin4I   HincII     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI    PflMI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SalI    SanDI     ScaI     SfcI
     SmaI     SmlI     StyI    TaqII     TatI     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI     BaeI     BbeI     BcgI     BclI
     BglI     BmtI     BplI    BsaBI    BsaHI    BsaWI    BsiWI    BspEI
    BspHI    BsrGI   BssHII   BstAPI   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EciI  EcoICRI    EcoRI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     MluI     NarI     NcoI     NdeI
     NheI     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XhoI     ZraI
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