Restriction map for pF1KA0090
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AspI        AviII       AvrII       BbrPI       
BbvCI       BcgI        BciVI       BfrI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaI        BsaWI       
BseAI       BsiWI       BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BssHII      Bst1107I    
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        EarI        
Eco47III    EcoO65I     EcoT22I     FseI        FspI        
HpaI        KpnI        Ksp632I     MamI        MfeI        
MluI        MroI        MslI        MunI        NaeI        
NdeI        NgoMIV      NheI        NruI        NsiI        
NspI        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SexAI       SfiI        SgrAI       SnaBI       SpeI        
SphI        SspI        StuI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AflIII       2882  ACGTGT               A^C[GA][CT]GT
AhdI         1774  GACAAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1755  GTGCAC               G^TGCAC
AlwNI         661  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1755  GTGCAC               G^TGCAC
Asp700I      1787  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1774  GACAAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1571  TGGCCA               TGG^CCA
BbeI          224  GGCGCC               GGCGC^C
BglII         215  AGATCT               A^GATCT
BsaAI        2881  TACGTG               [CT]AC^GT[GA]
BsiEI          56  CGGCCG               CG[GA][CT]^CG
Bsp1407I     2700  TGTACA               T^GTACA
BsrGI        2700  TGTACA               T^GTACA
BssSI        1132  CTCGTG               CACGAG(-5/-1)
BstAPI       1869  GCAGTCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2054  TTCGAA               TT^CGAA
DraI         1705  TTTAAA               TTT^AAA
EagI           56  CGGCCG               C^GGCCG
Eam1105I     1774  GACAAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          56  CGGCCG               C^GGCCG
Eco52I         56  CGGCCG               C^GGCCG
EcoNI        2613  CCTTCCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1582  GAATTC               G^AATTC
EcoRV        2045  GATATC               GAT^ATC
FauI         2636  CCCGC                CCCGC(4/6)
HincII        232  GTTGAC               GT[CT]^[GA]AC
HindII        232  GTTGAC               GT[CT]^[GA]AC
HindIII      1627  AAGCTT               A^AGCTT
KasI          224  GGCGCC               G^GCGCC
KspI           59  CCGCGG               CCGC^GG
MluNI        1571  TGGCCA               TGG^CCA
MscI         1571  TGGCCA               TGG^CCA
NarI          224  GGCGCC               GG^CGCC
NcoI         2534  CCATGG               C^CATGG
NotI           55  GCGGCCGC             GC^GGCCGC
NspV         2054  TTCGAA               TT^CGAA
PaeR7I       2831  CTCGAG               C^TCGAG
PvuII        2482  CAGCTG               CAG^CTG
SacII          59  CCGCGG               CCGC^GG
SbfI          405  CCTGCAGG             CCTGCA^GG
ScaI         2378  AGTACT               AGT^ACT
SfoI          224  GGCGCC               GGC^GCC
SfuI         2054  TTCGAA               TT^CGAA
Sse8387I      405  CCTGCAGG             CCTGCA^GG
SspBI        2700  TGTACA               T^GTACA
XcmI         2525  CCATCAGTGCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2831  CTCGAG               C^TCGAG
XmnI         1787  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI           64  GTCTAC               GT^[AC][GT]AC
             2836  GTCTAC              
AccII          60  CGCG                 CG^CG
             2244  CGCG                
AcyI          224  GGCGCC               G[GA]^CG[CT]C
             2287  GGCGTC              
AocI         1619  CCTCAGG              CC^T[ACGT]AGG
             2617  CCTAAGG             
ApaI          560  GGGCCC               GGGCC^C
              863  GGGCCC              
BamHI         148  GGATCC               G^GATCC
             2631  GGATCC              
BclI          290  TGATCA               T^GATCA
             2927  TGATCA              
BglI         1200  GCCTGAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2533  GCCATGGAGGC         
BsaHI         224  GGCGCC               G[GA]^CG[CT]C
             2287  GGCGTC              
BsmBI        1463  CGTCTC               CGTCTC(1/5)
              751  GAGACG              
BsmI         2750  GAATGC               GAATGC(1/-1)
             2352  GCATTC              
BsrBI        1188  GAGCGG               CCGCTC(-3/-3)
             1204  GAGCGG              
BstUI          60  CGCG                 CG^CG
             2244  CGCG                
Bsu36I       1619  CCTCAGG              CC^T[ACGT]AGG
             2617  CCTAAGG             
Eco81I       1619  CCTCAGG              CC^T[ACGT]AGG
             2617  CCTAAGG             
FbaI          290  TGATCA               T^GATCA
             2927  TGATCA              
HaeII         224  GGCGCC               [GA]GCGC^[CT]
             1856  AGCGCC              
Hin1I         224  GGCGCC               G[GA]^CG[CT]C
             2287  GGCGTC              
MvnI           60  CGCG                 CG^CG
             2244  CGCG                
PflMI         735  CCAAACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1658  CCATCCTGTGG         
PpuMI         824  GGGTCCT              [GA]G^G[AT]CC[CT]
             2492  AGGTCCT             
SacI          720  GAGCTC               GAGCT^C
             2422  GAGCTC              
SmaI          821  CCCGGG               CCC^GGG
              860  CCCGGG              
SmlI          481  CTCAAG               C^T[CT][GA]AG
             2831  CTCGAG              
Van91I        735  CCAAACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1658  CCATCCTGTGG         
XmaCI         821  CCCGGG               C^CCGGG
              860  CCCGGG              
XmaI          821  CCCGGG               C^CCGGG
              860  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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