Restriction map for pF1KA0090
 (Circular) (Six-base) MAPSORT of: pF1KA0090.seq  Check: 2253  from: 1  to: 6089



pF1KA0090  6089 bp

 With 182 enzymes: SgfI * 

                        September 25, 2009 11:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2693    2693 
   Size:       6089
 
AccI GT'mk_AC

Cuts at:    173    2945    3127     173 
   Size:       2772     182    3135

  Fragments arranged by size: 

                3135    2772     182
 
Acc65I G'GTAC_C

Cuts at:   3105    3105 
   Size:       6089
 
AclI AA'CG_TT

Cuts at:   5929    5929 
   Size:       6089
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    565     672    1169    1981    2553    2992    3914    4346    5093 
   Size:        107     497     812     572     439     922     432     747

Cuts at:   5093    5361     565 
   Size:        268    1293

  Fragments arranged by size: 

                1293     922     812     747     572     497     439     432
                 268     107
 
AflIII A'CryG_T

Cuts at:   2990    3441    4545    5522    2990 
   Size:        451    1104     977    3557

  Fragments arranged by size: 

                3557    1104     977     451
 
AgeI A'CCGG_T

Cuts at:   4477    4477 
   Size:       6089
 
AhdI GACnn_n'nnGTC

Cuts at:   1887    1887 
   Size:       6089
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    719     751     719 
   Size:         32    6057
 
AlwNI CAG_nnn'CTG

Cuts at:    774    3394    4961     774 
   Size:       2620    1567    1902

  Fragments arranged by size: 

                2620    1902    1567
 
ApaI G_GGCC'C

Cuts at:    672     975     672 
   Size:        303    5786
 
ApaLI G'TGCA_C

Cuts at:   1863    4859    1863 
   Size:       2996    3093
 
ApoI r'AATT_y

Cuts at:    244     306     907    1519    1690    3093    5442    5698     244 
   Size:         62     601     612     171    1403    2349     256     635

  Fragments arranged by size: 

                2349    1403     635     612     601     256     171      62
            
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6089
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6089
 
AvaI C'yCGr_G

Cuts at:    825     929     968    2752    2883    2939    3109     825 
   Size:        104      39    1784     131      56     170    3805

  Fragments arranged by size: 

                3805    1784     170     131     104      56      39
 
BamHI G'GATC_C

Cuts at:    256    2739    3114     256 
   Size:       2483     375    3231

  Fragments arranged by size: 

                3231    2483     375
 
BanI G'GyrC_C

Cuts at:    332     584    1762    3105    3519    3767    3802     332 
   Size:        252    1178    1343     414     248      35    2619

  Fragments arranged by size: 

                2619    1343    1178     414     252     248      35
 
BanII G_rGCy'C

Cuts at:    672     832     975    2534    3103    4133     672 
   Size:        160     143    1559     569    1030    2628

  Fragments arranged by size: 

                2628    1559    1030     569     160     143
 
BbeI G_GCGC'C

Cuts at:    336    3523    3771     336 
   Size:       3187     248    2654

  Fragments arranged by size: 

                3187    2654     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    185     556    1102    1905     185 
   Size:        371     546     803    4369

  Fragments arranged by size: 

                4369     803     546     371
 
BciVI GTATCCnnnnn_n'

Cuts at:   3987    4748    6089    3987 
   Size:        761    1341    3987

  Fragments arranged by size: 

                3987    1341     761
 
BclI T'GATC_A

Cuts at:    398    3035    3609     398 
   Size:       2637     574    2878

  Fragments arranged by size: 

                2878    2637     574
 
BglI GCCn_nnn'nGGC

Cuts at:    112    1314    2647     112 
   Size:       1202    1333    3554

  Fragments arranged by size: 

                3554    1333    1202
 
BglII A'GATC_T

Cuts at:    323    3604    5641     323 
   Size:       3281    2037     771

  Fragments arranged by size: 

                3281    2037     771
 
BlpI GC'TnA_GC

Cuts at:   3201    3201 
   Size:       6089
 
Bme1580I G_kGCm'C

Cuts at:    352     587     672     975    1500    1867    2545    3714    3807 
   Size:        235      85     303     525     367     678    1169      93

Cuts at:   3807    4863     352 
   Size:       1056    1578

  Fragments arranged by size: 

                1578    1169    1056     678     525     367     303     235
                  93      85
 
BmrI ACTGGGnnnn_n'

Cuts at:    326     471     949     969    1097    1502    1948    1987    2411 
   Size:        145     478      20     128     405     446      39     424

Cuts at:   2411    2481    3301    3382    3717    5861     326 
   Size:         70     820      81     335    2144     554

  Fragments arranged by size: 

                2144     820     554     478     446     424     405     335
                 145     128      81      70      39      20
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1319    1773    4456    1319 
   Size:        454    2683    2952

  Fragments arranged by size: 

                2952    2683     454
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    561     800     877    1684    2548    2590    2726    2786    2911 
   Size:        239      77     807     864      42     136      60     125

Cuts at:   2911     561 
   Size:       3739

  Fragments arranged by size: 

                3739     864     807     239     136     125      77      60
                  42
 
Bpu10I CC'TnA_GC

Cuts at:   1310    1310 
   Size:       6089
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    574    3265    4144    4636    4934     574 
   Size:       2691     879     492     298    1729

  Fragments arranged by size: 

                2691    1729     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   5793    5793 
   Size:       6089
 
BsaAI yAC'GTr

Cuts at:   2991    3442    2991 
   Size:        451    5638
 
BsaBI GATnn'nnATC

Cuts at:      6    3603    5640       6 
   Size:       3597    2037     455

  Fragments arranged by size: 

                3597    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    333    2396    3520    3768    4470    5966     333 
   Size:       2063    1124     248     702    1496     456

  Fragments arranged by size: 

                2063    1496    1124     702     456     248
 
BsaWI w'CCGG_w

Cuts at:   3278    3799    4477    4751    4898    5309    3278 
   Size:        521     678     274     147     411    4058

  Fragments arranged by size: 

                4058     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    313     343     548     578     719     749     824     854     313 
   Size:         30     205      30     141      30      75      30    5548

  Fragments arranged by size: 

                5548     205     141      75      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1456    1797    2409    2794    1456 
   Size:        341     612     385    4751

  Fragments arranged by size: 

                4751     612     385     341
 
BseYI C'CCAG_C

Cuts at:    944    1545    2588    3514    4272    4849    5345     944 
   Size:        601    1043     926     758     577     496    1688

  Fragments arranged by size: 

                1688    1043     926     758     601     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    367     970    1393    2446     367 
   Size:        603     423    1053    4010

  Fragments arranged by size: 

                4010    1053     603     423
 
BsiEI CG_ry'CG

Cuts at:    104     167    3631    3677    4885     104 
   Size:         63    3464      46    1208    1308

  Fragments arranged by size: 

                3464    1308    1208      63      46
 
BsiHKAI G_wGCw'C

Cuts at:    832    1867    2258    2300    2347    2534    3103    3881    4071 
   Size:       1035     391      42      47     187     569     778     190

Cuts at:   4071    4863     832 
   Size:        792    2058

  Fragments arranged by size: 

                2058    1035     792     778     569     391     190     187
                  47      42
 
BsmI GAATG_Cn'

Cuts at:   2460    2864    2460 
   Size:        404    5685
 
BsmBI CGTCTCn'nnnn_

Cuts at:    853    1577     853 
   Size:        724    5365
 
Bsp1286I G_dGCh'C

Cuts at:    352     587     672     832     975    1500    1867    2258    2300 
   Size:        235      85     160     143     525     367     391      42

Cuts at:   2300    2347    2534    2545    3103    3714    3807    3881    4071 
   Size:         47     187      11     558     611      93      74     190

Cuts at:   4071    4133    4863     352 
   Size:         62     730    1578

  Fragments arranged by size: 

                1578     730     611     558     525     391     367     235
                 190     187     160     143      93      85      74      62
                  47      42      11
 
BspEI T'CCGG_A

Cuts at:   5309    5309 
   Size:       6089
 
BspHI T'CATG_A

Cuts at:   5265    5265 
   Size:       6089
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    732    2330    2693    3139    3353    3655    4036     732 
   Size:       1598     363     446     214     302     381    2785

  Fragments arranged by size: 

                2785    1598     446     381     363     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1298    1314    4381    4435    5919    6084      50 
   Size:       1248      16    3067      54    1484     165      55

  Fragments arranged by size: 

                3067    1484    1248     165      55      54      16
 
BsrDI GCAATG_nn'

Cuts at:   4001    4001 
   Size:       6089
 
BsrFI r'CCGG_y

Cuts at:   4087    4268    4477    4087 
   Size:        181     209    5699

  Fragments arranged by size: 

                5699     209     181
 
BsrGI T'GTAC_A

Cuts at:   2808    2808 
   Size:       6089
 
BssSI C'ACGA_G

Cuts at:   1240    4360    4718    1240 
   Size:       3120     358    2611

  Fragments arranged by size: 

                3120    2611     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1983    3466    1983 
   Size:       1483    4606
 
BstBI TT'CG_AA

Cuts at:   2163    4450    2163 
   Size:       2287    3802
 
BstXI CCAn_nnnn'nTGG

Cuts at:    267     499     957    2325    2904     267 
   Size:        232     458    1368     579    3452

  Fragments arranged by size: 

                3452    1368     579     458     232
 
BstYI r'GATC_y

Cuts at:      1     256     323     881    2169    2739    2756    3114    3581 
   Size:        255      67     558    1288     570      17     358     467

Cuts at:   3581    3604    3939    4185    5197    5283    5295    5641       1 
   Size:         23     335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1288    1012     570     558     467     449     358     346
                 335     255     246      86      67      23      17      12
            
 
Bsu36I CC'TnA_GG

Cuts at:   1728    2726    1728 
   Size:        998    5091
 
BtgI C'CryG_G

Cuts at:    107     167     760    2330    2642     107 
   Size:         60     593    1570     312    3554

  Fragments arranged by size: 

                3554    1570     593     312      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    519    4039    4220    4383     519 
   Size:       3520     181     163    2225

  Fragments arranged by size: 

                3520    2225     181     163
 
BtsI GCAGTG_nn'

Cuts at:    886    2256    2298    3332     886 
   Size:       1370      42    1034    3643

  Fragments arranged by size: 

                3643    1370    1034      42
 
DraI TTT'AAA

Cuts at:   1815    3088    1815 
   Size:       1273    4816
 
DrdI GACnn_nn'nnGTC

Cuts at:   3795    4653    3795 
   Size:        858    5231
 
EaeI y'GGCC_r

Cuts at:    164    1679    2327    3674    3848    4239    4266    5611     164 
   Size:       1515     648    1347     174     391      27    1345     642

  Fragments arranged by size: 

                1515    1347    1345     648     642     391     174      27
            
 
EagI C'GGCC_G

Cuts at:    164    3674     164 
   Size:       3510    2579
 
EarI CTCTTCn'nnn_

Cuts at:   4112    4322    4112 
   Size:        210    5879
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4297    4607    4753    5899    4297 
   Size:        310     146    1146    4487

  Fragments arranged by size: 

                4487    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    830    2532    3101     830 
   Size:       1702     569    3818

  Fragments arranged by size: 

                3818    1702     569
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    561     565     672     800     877    1169    1684    1981    2548 
   Size:          4     107     128      77     292     515     297     567

Cuts at:   2548    2553    2590    2726    2786    2911    2992    3914    4346 
   Size:          5      37     136      60     125      81     922     432

Cuts at:   4346    5093    5361     561 
   Size:        747     268    1289

  Fragments arranged by size: 

                1289     922     747     567     515     432     297     292
                 268     136     128     125     107      81      77      60
                  37       5       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2725    2725 
   Size:       6089
 
EcoO109I rG'GnC_Cy

Cuts at:    453     669     933    2443    2601    3228     453 
   Size:        216     264    1510     158     627    3314

  Fragments arranged by size: 

                3314    1510     627     264     216     158
 
EcoRI G'AATT_C

Cuts at:   1690    3093    1690 
   Size:       1403    4686
 
EcoRV GAT'ATC

Cuts at:   2155    2155 
   Size:       6089
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4108    4108 
   Size:       6089
 
FspI TGC'GCA

Cuts at:   3870    3870 
   Size:       6089
 
HaeII r_GCGC'y

Cuts at:    336    1968    3523    3771    4793    5772     336 
   Size:       1632    1555     248    1022     979     653

  Fragments arranged by size: 

                1632    1555    1022     979     653     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    407     439     814     846    1368    1400    1873    1905    2838 
   Size:         32     375      32     522      32     473      32     933

Cuts at:   2838    2870    3931    3961    3963    3993     407 
   Size:         32    1061      30       2      30    2503

  Fragments arranged by size: 

                2503    1061     933     522     473     375      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:    342    3128     342 
   Size:       2786    3303
 
HindIII A'AGCT_T

Cuts at:   1735    1735 
   Size:       6089
 
KasI G'GCGC_C

Cuts at:    332    3519    3767     332 
   Size:       3187     248    2654

  Fragments arranged by size: 

                3187    2654     248
 
KpnI G_GTAC'C

Cuts at:   3109    3109 
   Size:       6089
 
MluI A'CGCG_T

Cuts at:   5522    5522 
   Size:       6089
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    948    1102    4760    4944     948 
   Size:        154    3658     184    2093

  Fragments arranged by size: 

                3658    2093     184     154
 
MscI TGG'CCA

Cuts at:   1681    3850    1681 
   Size:       2169    3920
 
MslI CAynn'nnrTG

Cuts at:   4078    4205    4078 
   Size:        127    5962
 
MspA1I CmG'CkG

Cuts at:    169    2220    2592    3200    3514    3874    4887    5132     169 
   Size:       2051     372     608     314     360    1013     245    1126

  Fragments arranged by size: 

                2051    1126    1013     608     372     360     314     245
            
 
NaeI GCC'GGC

Cuts at:   4270    4270 
   Size:       6089
 
NarI GG'CG_CC

Cuts at:    333    3520    3768     333 
   Size:       3187     248    2654

  Fragments arranged by size: 

                3187    2654     248
 
NcoI C'CATG_G

Cuts at:    107    2642     107 
   Size:       2535    3554
 
NgoMIV G'CCGG_C

Cuts at:   4268    4268 
   Size:       6089
 
NotI GC'GGCC_GC

Cuts at:    164     164 
   Size:       6089
 
NspI r_CATG'y

Cuts at:   3142    3642    4549    3142 
   Size:        500     907    4682

  Fragments arranged by size: 

                4682     907     500
 
PasI CC'CwG_GG

Cuts at:   2268    2268 
   Size:       6089
 
PciI A'CATG_T

Cuts at:   4545    4545 
   Size:       6089
 
PflMI CCAn_nnn'nTGG

Cuts at:    849    1772     849 
   Size:        923    5166
 
PmeI GTTT'AAAC

Cuts at:   3088    3088 
   Size:       6089
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    719     751     819     851    1824    1856    5228    5260     719 
   Size:         32      68      32     973      32    3372      32    1548

  Fragments arranged by size: 

                3372    1548     973      68      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:    933    2601     933 
   Size:       1668    4421
 
PspOMI G'GGCC_C

Cuts at:    668     971     668 
   Size:        303    5786
 
PspXI vC'TCGA_Gb

Cuts at:   2939    2939 
   Size:       6089
 
PstI C_TGCA'G

Cuts at:    518     770     893    1019    1110    1406    3136    3821     518 
   Size:        252     123     126      91     296    1730     685    2786

  Fragments arranged by size: 

                2786    1730     685     296     252     126     123      91
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6089
 
PvuII CAG'CTG

Cuts at:   2592    3514    3874    2592 
   Size:        922     360    4807

  Fragments arranged by size: 

                4807     922     360
 
RsrII CG'GwC_CG

Cuts at:   4284    4284 
   Size:       6089
 
SacI G_AGCT'C

Cuts at:    832    2534    3103     832 
   Size:       1702     569    3818

  Fragments arranged by size: 

                3818    1702     569
 
SacII CC_GC'GG

Cuts at:    170     170 
   Size:       6089
 
SalI G'TCGA_C

Cuts at:   3126    3126 
   Size:       6089
 
SapI GCTCTTCn'nnn_

Cuts at:   4112    4322    4112 
   Size:        210    5879
 
SbfI CC_TGCA'GG

Cuts at:    518    3136     518 
   Size:       2618    3471
 
ScaI AGT'ACT

Cuts at:   2488    5606    2488 
   Size:       3118    2971
 
SfcI C'TryA_G

Cuts at:     33     514     766     889    1015    1106    1402    3132    3817 
   Size:        481     252     123     126      91     296    1730     685

Cuts at:   3817    4810    5001      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1730    1121     993     685     481     296     252     191
                 126     123      91
 
SfoI GGC'GCC

Cuts at:    334    3521    3769     334 
   Size:       3187     248    2654

  Fragments arranged by size: 

                3187    2654     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6089
 
SmaI CCC'GGG

Cuts at:    931     970    3111     931 
   Size:         39    2141    3909

  Fragments arranged by size: 

                3909    2141      39
 
SmlI C'TyrA_G

Cuts at:    589    2939    3244    4159    4651    4913     589 
   Size:       2350     305     915     492     262    1765

  Fragments arranged by size: 

                2350    1765     915     492     305     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6089
 
SphI G_CATG'C

Cuts at:   3142    3642    3142 
   Size:        500    5589
 
StyI C'CwwG_G

Cuts at:    107     239    2015    2498    2642    3223    3577     107 
   Size:        132    1776     483     144     581     354    2619

  Fragments arranged by size: 

                2619    1776     581     483     354     144     132
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    585    2463    3672    3805    4264    4473     585 
   Size:       1878    1209     133     459     209    2201

  Fragments arranged by size: 

                2201    1878    1209     459     209     133
 
TatI w'GTAC_w

Cuts at:   2214    2486    2808    5604    2214 
   Size:        272     322    2796    2699

  Fragments arranged by size: 

                2796    2699     322     272
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    313     345     380     412     548     580     824     856    2043 
   Size:         32      35      32     136      32     244      32    1187

Cuts at:   2043    2075    2248    2280    5606    5638     313 
   Size:         32     173      32    3326      32     764

  Fragments arranged by size: 

                3326    1187     764     244     173     136      35      32
                  32      32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3886    3886 
   Size:       6089
 
XbaI T'CTAG_A

Cuts at:   3120    3120 
   Size:       6089
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2640    3584    2640 
   Size:        944    5145
 
XhoI C'TCGA_G

Cuts at:   2939    2939 
   Size:       6089
 
XmaI C'CCGG_G

Cuts at:    929     968    3109     929 
   Size:         39    2141    3909

  Fragments arranged by size: 

                3909    2141      39
 
XmnI GAAnn'nnTTC

Cuts at:   1899    1899 
   Size:       6089
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AlfI     AscI    AvrII     BaeI
    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BsiWI   BssHII   BstEII
  BstZ17I     ClaI    CspCI   DraIII     FseI    FspAI     HpaI     MfeI
     NdeI     NheI     NruI     NsiI     PacI     PfoI     PmlI    PshAI
     PsiI     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     ZraI
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