Restriction map for pF1KA0015
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AhdI        AlwI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BsaBI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MboI        MfeI        MflI        MluI        
MunI        NarI        NdeI        NdeII       NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII        1090  GACGTC               GACGT^C
AccIII        485  TCCGGA               T^CCGGA
AcsI          280  GAATTC               [GA]^AATT[CT]
AcyI         1090  GACGTC               G[GA]^CG[CT]C
Alw44I        637  GTGCAC               G^TGCAC
ApaLI         637  GTGCAC               G^TGCAC
ApoI          280  GAATTC               [GA]^AATT[CT]
BbvCI          46  GCTGAGG              CCTCAGC(-5/-2)
BsaHI        1090  GACGTC               G[GA]^CG[CT]C
BsaWI         485  TCCGGA               [AT]^CCGG[AT]
BseAI         485  TCCGGA               T^CCGGA
BsgI          799  CTGCAC               GTGCAG(16/14)
BsmBI         969  CGTCTC               CGTCTC(1/5)
BspEI         485  TCCGGA               T^CCGGA
BsrDI         783  CATTGC               GCAATG(2/0)
BssHII        901  GCGCGC               G^CGCGC
BstAPI       1308  GCAGGACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoRI         280  GAATTC               G^AATTC
HaeII        1038  GGCGCT               [GA]GCGC^[CT]
Hin1I        1090  GACGTC               G[GA]^CG[CT]C
HincII        952  GTCAAC               GT[CT]^[GA]AC
HindII        952  GTCAAC               GT[CT]^[GA]AC
HphI          855  GGTGA                GGTGA(8/7)
MroI          485  TCCGGA               T^CCGGA
MseI          389  TTAA                 T^TAA
NaeI          409  GCCGGC               GCC^GGC
NgoMIV        409  GCCGGC               G^CCGGC
PflMI         366  CCAAAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          950  GAGTC                GAGTC(4/5)
PstI          751  CTGCAG               CTGCA^G
PvuII         256  CAGCTG               CAG^CTG
TfiI          820  GATTC                G^A[AT]TC
Tru9I         389  TTAA                 T^TAA
Tsp509I       281  AATT                 ^AATT
Van91I        366  CCAAAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccII         571  CGCG                 CG^CG
              902  CGCG                
AflIII        518  ACGTGT               A^C[GA][CT]GT
             1007  ACGTGT              
AocI          679  CCTGAGG              CC^T[ACGT]AGG
             1236  CCTCAGG             
AspHI         142  GTGCTC               G[AT]GC[AT]^C
              637  GTGCAC              
BalI           35  TGGCCA               TGG^CCA
              182  TGGCCA              
BanI          429  GGTGCC               G^G[CT][GA]CC
              760  GGCACC              
BbrPI         517  CACGTG               CAC^GTG
              634  CACGTG              
BbsI          991  GTCTTC               GAAGAC(2/6)
             1231  GTCTTC              
BlpI          144  GCTCAGC              GC^T[ACGT]AGC
              175  GCTGAGC             
Bpu1102I      144  GCTCAGC              GC^T[ACGT]AGC
              175  GCTGAGC             
BpuAI         991  GTCTTC               GAAGAC(2/6)
             1231  GTCTTC              
BsiHKAI       142  GTGCTC               G[AT]GC[AT]^C
              637  GTGCAC              
BspMI        1061  ACCTGC               ACCTGC(4/8)
              426  GCAGGT              
BsrFI         409  GCCGGC               [GA]^CCGG[CT]
              512  ACCGGC              
BsrI           38  CCAGT                ACTGG(1/-1)
              414  CCAGT               
BstUI         571  CGCG                 CG^CG
              902  CGCG                
Bsu36I        679  CCTGAGG              CC^T[ACGT]AGG
             1236  CCTCAGG             
CelII         144  GCTCAGC              GC^T[ACGT]AGC
              175  GCTGAGC             
Cfr10I        409  GCCGGC               [GA]^CCGG[CT]
              512  ACCGGC              
DsaI          121  CCATGG               C^C[GA][CT]GG
              185  CCATGG              
Eco81I        679  CCTGAGG              CC^T[ACGT]AGG
             1236  CCTCAGG             
EcoT14I       121  CCATGG               C^C[AT][AT]GG
              185  CCATGG              
HinfI         820  GATTC                G^A[ACGT]TC
              950  GAGTC               
MaeIII        349  GTGAC                ^GT[ACGT]AC
              596  GTCAC               
MluNI          35  TGGCCA               TGG^CCA
              182  TGGCCA              
MscI           35  TGGCCA               TGG^CCA
              182  TGGCCA              
MslI          765  CACAGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1218  CACGGTCATG          
MvnI          571  CGCG                 CG^CG
              902  CGCG                
NcoI          121  CCATGG               C^CATGG
              185  CCATGG              
PmaCI         517  CACGTG               CAC^GTG
              634  CACGTG              
PmlI          517  CACGTG               CAC^GTG
              634  CACGTG              
SapI           21  GAAGAGC              GCTCTTC(1/4)
              543  GCTCTTC             
SfcI          262  CTACAG               C^T[GA][CT]AG
              751  CTGCAG              
StyI          121  CCATGG               C^C[AT][AT]GG
              185  CCATGG              
Tsp45I        349  GTGAC                ^GT[GC]AC
              596  GTCAC               
XcmI          185  CCATGGGCTTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1104  CCAGGAAGTTGTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaAI       BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DraII       
EaeI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        Ksp632I     MaeII       
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PpuMI       
RsaI        Sau96I      ScrFI       SfaNI       SmaI        
TseI        TspRI       XmaCI       XmaI        
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