Restriction map for pF1KA0015
 (Linear) (Six-base) MAPSORT of: KIAA0015.seq  Check: 4821  from: 1  to: 1362



KIAA0015.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     417    1362 
   Size:        417     945
 
AatII G_ACGT'C

Cuts at:      0    1094    1362 
   Size:       1094     268
 
AflIII A'CryG_T

Cuts at:      0     518    1007    1362 
   Size:        518     489     355

  Fragments arranged by size: 

                 518     489     355
 
AleI CACnn'nnGTG

Cuts at:      0     769    1362 
   Size:        769     593
 
AlwNI CAG_nnn'CTG

Cuts at:      0     465     679    1336    1362 
   Size:        465     214     657      26

  Fragments arranged by size: 

                 657     465     214      26
 
ApaLI G'TGCA_C

Cuts at:      0     637    1362 
   Size:        637     725
 
ApoI r'AATT_y

Cuts at:      0     280    1362 
   Size:        280    1082
 
AvaI C'yCGr_G

Cuts at:      0     443     814    1034    1190    1362 
   Size:        443     371     220     156     172

  Fragments arranged by size: 

                 443     371     220     172     156
 
BanI G'GyrC_C

Cuts at:      0     429     760    1362 
   Size:        429     331     602

  Fragments arranged by size: 

                 602     429     331
 
BanII G_rGCy'C

Cuts at:      0      15     690    1051    1160    1204    1362 
   Size:         15     675     361     109      44     158

  Fragments arranged by size: 

                 675     361     158     109      44      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     984    1224    1362 
   Size:        984     240     138

  Fragments arranged by size: 

                 984     240     138
 
BbvCI CC'TCA_GC

Cuts at:      0      47    1362 
   Size:         47    1315
 
BlpI GC'TnA_GC

Cuts at:      0     145     176    1362 
   Size:        145      31    1186

  Fragments arranged by size: 

                1186     145      31
 
Bme1580I G_kGCm'C

Cuts at:      0     641    1362 
   Size:        641     721
 
BmgBI CAC'GTC

Cuts at:      0     804    1362 
   Size:        804     558
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1218    1362 
   Size:       1218     144
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     957     966    1279    1305    1362 
   Size:         29     928       9     313      26      57

  Fragments arranged by size: 

                 928     313      57      29      26       9
 
Bpu10I CC'TnA_GC

Cuts at:      0      47    1362 
   Size:         47    1315
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      46     474    1333    1362 
   Size:         46     428     859      29

  Fragments arranged by size: 

                 859     428      46      29
 
BsaAI yAC'GTr

Cuts at:      0     519     636    1008    1362 
   Size:        519     117     372     354

  Fragments arranged by size: 

                 519     372     354     117
 
BsaHI Gr'CG_yC

Cuts at:      0    1091    1362 
   Size:       1091     271
 
BsaWI w'CCGG_w

Cuts at:      0     485    1362 
   Size:        485     877
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1303    1332    1333    1362 
   Size:       1303      29       1      29

  Fragments arranged by size: 

                1303      29      29       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64     169     328     331     346    1186    1317    1362 
   Size:         64     105     159       3      15     840     131      45

  Fragments arranged by size: 

                 840     159     131     105      64      45      15       3
            
 
BseYI C'CCAG_C

Cuts at:      0      90    1362 
   Size:         90    1272
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     784    1362 
   Size:        784     578
 
BsiHKAI G_wGCw'C

Cuts at:      0     146     641    1362 
   Size:        146     495     721

  Fragments arranged by size: 

                 721     495     146
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     975    1362 
   Size:        975     387
 
Bsp1286I G_dGCh'C

Cuts at:      0      15     146     641     690    1051    1160    1204    1362 
   Size:         15     131     495      49     361     109      44     158

  Fragments arranged by size: 

                 495     361     158     131     109      49      44      15
            
 
BspEI T'CCGG_A

Cuts at:      0     485    1362 
   Size:        485     877
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     417    1070    1362 
   Size:        417     653     292

  Fragments arranged by size: 

                 653     417     292
 
BsrBI CCG'CTC

Cuts at:      0     747     758    1197    1362 
   Size:        747      11     439     165

  Fragments arranged by size: 

                 747     439     165      11
 
BsrDI GCAATG_nn'

Cuts at:      0     782    1362 
   Size:        782     580
 
BsrFI r'CCGG_y

Cuts at:      0     409     512    1362 
   Size:        409     103     850

  Fragments arranged by size: 

                 850     409     103
 
BssHII G'CGCG_C

Cuts at:      0     901    1362 
   Size:        901     461
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1314    1362 
   Size:       1314      48
 
Bsu36I CC'TnA_GG

Cuts at:      0     680    1237    1362 
   Size:        680     557     125

  Fragments arranged by size: 

                 680     557     125
 
BtgI C'CryG_G

Cuts at:      0     121     185    1362 
   Size:        121      64    1177

  Fragments arranged by size: 

                1177     121      64
 
BtsI GCAGTG_nn'

Cuts at:      0     466    1362 
   Size:        466     896
 
EaeI y'GGCC_r

Cuts at:      0      35     182     411     965    1362 
   Size:         35     147     229     554     397

  Fragments arranged by size: 

                 554     397     229     147      35
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     305     550    1362 
   Size:         16     289     245     812

  Fragments arranged by size: 

                 812     289     245      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     957     966    1279    1305    1362 
   Size:         29     928       9     313      26      57

  Fragments arranged by size: 

                 928     313      57      29      26       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     111     129     210     342     959    1242    1284    1362 
   Size:        111      18      81     132     617     283      42      78

  Fragments arranged by size: 

                 617     283     132     111      81      78      42      18
            
 
EcoRI G'AATT_C

Cuts at:      0     280    1362 
   Size:        280    1082
 
HaeII r_GCGC'y

Cuts at:      0    1042    1362 
   Size:       1042     320
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1303    1332    1335    1362 
   Size:       1303      29       3      27

  Fragments arranged by size: 

                1303      29      27       3
 
HincII GTy'rAC

Cuts at:      0     954    1362 
   Size:        954     408
 
MscI TGG'CCA

Cuts at:      0      37     184    1362 
   Size:         37     147    1178

  Fragments arranged by size: 

                1178     147      37
 
MslI CAynn'nnrTG

Cuts at:      0     769    1222    1362 
   Size:        769     453     140

  Fragments arranged by size: 

                 769     453     140
 
MspA1I CmG'CkG

Cuts at:      0     258     456     628    1154    1362 
   Size:        258     198     172     526     208

  Fragments arranged by size: 

                 526     258     208     198     172
 
NaeI GCC'GGC

Cuts at:      0     411    1362 
   Size:        411     951
 
NcoI C'CATG_G

Cuts at:      0     121     185    1362 
   Size:        121      64    1177

  Fragments arranged by size: 

                1177     121      64
 
NgoMIV G'CCGG_C

Cuts at:      0     409    1362 
   Size:        409     953
 
PasI CC'CwG_GG

Cuts at:      0     133     296    1362 
   Size:        133     163    1066

  Fragments arranged by size: 

                1066     163     133
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     372    1362 
   Size:        372     990
 
PfoI T'CCnGG_A

Cuts at:      0      65    1362 
   Size:         65    1297
 
PmlI CAC'GTG

Cuts at:      0     519     636    1362 
   Size:        519     117     726

  Fragments arranged by size: 

                 726     519     117
 
PpuMI rG'GwC_Cy

Cuts at:      0     111     959    1242    1284    1362 
   Size:        111     848     283      42      78

  Fragments arranged by size: 

                 848     283     111      78      42
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     559     591    1362 
   Size:        559      32     771

  Fragments arranged by size: 

                 771     559      32
 
PstI C_TGCA'G

Cuts at:      0     755    1362 
   Size:        755     607
 
PvuII CAG'CTG

Cuts at:      0     258    1362 
   Size:        258    1104
 
SanDI GG'GwC_CC

Cuts at:      0     959    1242    1284    1362 
   Size:        959     283      42      78

  Fragments arranged by size: 

                 959     283      78      42
 
SapI GCTCTTCn'nnn_

Cuts at:      0      16     550    1362 
   Size:         16     534     812

  Fragments arranged by size: 

                 812     534      16
 
SfcI C'TryA_G

Cuts at:      0     262     751    1362 
   Size:        262     489     611

  Fragments arranged by size: 

                 611     489     262
 
SmaI CCC'GGG

Cuts at:      0     445    1036    1192    1362 
   Size:        445     591     156     170

  Fragments arranged by size: 

                 591     445     170     156
 
SrfI GCCC'GGGC

Cuts at:      0     445    1362 
   Size:        445     917
 
StyI C'CwwG_G

Cuts at:      0     121     185    1362 
   Size:        121      64    1177

  Fragments arranged by size: 

                1177     121      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     907    1362 
   Size:        907     455
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     839     871    1362 
   Size:        839      32     491

  Fragments arranged by size: 

                 839     491      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     192    1111    1362 
   Size:        192     919     251

  Fragments arranged by size: 

                 919     251     192
 
XmaI C'CCGG_G

Cuts at:      0     443    1034    1190    1362 
   Size:        443     591     156     172

  Fragments arranged by size: 

                 591     443     172     156
 
ZraI GAC'GTC

Cuts at:      0    1092    1362 
   Size:       1092     270
 

 Enzymes that do cut:

     AarI    AatII   AflIII     AleI    AlwNI    ApaLI     ApoI     AvaI
     BanI    BanII     BbsI    BbvCI     BlpI Bme1580I    BmgBI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII   BstAPI   Bsu36I     BtgI     BtsI     EaeI
     EarI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I   HincII     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     PasI    PflMI     PfoI
     PmlI    PpuMI     PsrI     PstI    PvuII    SanDI     SapI     SfcI
     SmaI     SrfI     StyI    TaqII     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BmrI     BmtI    BpuEI    BsaBI    BsiEI    BsiWI     BsmI    BspHI
    BsrGI    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NarI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII
     SalI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmnI
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