################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI StuI SwaI TaqI Tsp509I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AciI 901 GCGG CCGC(-3/-1) AflIII 467 ACATGT A^C[GA][CT]GT AhdI 910 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 967 CCTCAGG CC^T[ACGT]AGG AspEI 910 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 362 TGATCA T^GATCA BcnI 452 CCCGG CC^[GC]GG BsaI 237 GGTCTC GGTCTC(1/5) BseRI 853 CTCCTC GAGGAG(10/8) BsiEI 773 CGACCG CG[GA][CT]^CG BsmBI 777 CGTCTC CGTCTC(1/5) Bsp1407I 1070 TGTACA T^GTACA BspLU11I 467 ACATGT A^CATGT BspMI 806 ACCTGC ACCTGC(4/8) BsrGI 1070 TGTACA T^GTACA BsrI 341 CCAGT ACTGG(1/-1) BstYI 1099 AGATCC [GA]^GATC[CT] Bsu36I 967 CCTCAGG CC^T[ACGT]AGG Cac8I 784 GCATGC GC[ACGT]^[ACGT]GC CfoI 210 GCGC GCG^C Eam1105I 910 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 967 CCTCAGG CC^T[ACGT]AGG EcoRV 1060 GATATC GAT^ATC FbaI 362 TGATCA T^GATCA HapII 453 CCGG C^CGG HgaI 270 GACGC GACGC(5/10) HhaI 210 GCGC GCG^C HinP1I 210 GCGC G^CGC HincII 529 GTCAAC GT[CT]^[GA]AC HindII 529 GTCAAC GT[CT]^[GA]AC HindIII 740 AAGCTT A^AGCTT HpaII 453 CCGG C^CGG MflI 1099 AGATCC [GA]^GATC[CT] MseI 197 TTAA T^TAA MspA1I 400 CAGCTG C[AC]G^C[GT]G MspI 453 CCGG C^CGG MwoI 449 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 452 CCCGG CC^[GC]GG PflFI 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 400 CAGCTG CAG^CTG SacI 456 GAGCTC GAGCT^C SbfI 514 CCTGCAGG CCTGCA^GG SmlI 762 CTCAAG C^T[CT][GA]AG SphI 784 GCATGC GCATG^C Sse8387I 514 CCTGCAGG CCTGCA^GG SspBI 1070 TGTACA T^GTACA TfiI 993 GAATC G^A[AT]TC Tru9I 197 TTAA T^TAA Tth111I 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 341 CCAGTGACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1099 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 471 GTAC GT^AC 1071 GTAC AlwI 769 GATCC GGATC(4/5) 1100 GATCC Asp700I 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 665 GAAACCCTTC AspHI 456 GAGCTC G[AT]GC[AT]^C 879 GTGCTC BalI 58 TGGCCA TGG^CCA 735 TGGCCA BsgI 178 CTGCAC GTGCAG(16/14) 808 CTGCAC BsiHKAI 456 GAGCTC G[AT]GC[AT]^C 879 GTGCTC BstXI 410 CCACGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 616 CCATACGAGTGG DsaI 410 CCACGG C^C[GA][CT]GG 573 CCACGG EaeI 58 TGGCCA [CT]^GGCC[GA] 735 TGGCCA EcoT14I 242 CCAAGG C^C[AT][AT]GG 524 CCAAGG MaeII 504 ACGT A^CGT 731 ACGT MluNI 58 TGGCCA TGG^CCA 735 TGGCCA MscI 58 TGGCCA TGG^CCA 735 TGGCCA MslI 226 CACCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 617 CATACGAGTG NspI 467 ACATGT [GA]CATG^[CT] 784 GCATGC RsaI 471 GTAC GT^AC 1071 GTAC SfaNI 296 GATGC GCATC(5/9) 590 GATGC StyI 242 CCAAGG C^C[AT][AT]GG 524 CCAAGG XmnI 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 665 GAAACCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AluI ApaI AvaII BanII BbvI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV PleI PpuMI PstI Sau3AI Sau96I ScrFI SfcI TseI Tsp45I TspRI