################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AhdI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstYI BstZ17I CfoI ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI StuI SwaI Tsp509I Van91I XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflIII 467 ACATGT A^C[GA][CT]GT AgeI 712 ACCGGT A^CCGGT AocI 892 CCTCAGG CC^T[ACGT]AGG AvaI 633 CTCGAG C^[CT]CG[GA]G BalI 58 TGGCCA TGG^CCA BlpI 1015 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1015 GCTGAGC GC^T[ACGT]AGC BsaI 237 GGTCTC GGTCTC(1/5) BsaWI 712 ACCGGT [AT]^CCGG[AT] BsgI 178 CTGCAC GTGCAG(16/14) BsoBI 633 CTCGAG C^[CT]CG[GA]G Bsp1407I 995 TGTACA T^GTACA BspLU11I 467 ACATGT A^CATGT BspMI 728 ACCTGC ACCTGC(4/8) BsrDI 141 GCAATG GCAATG(2/0) BsrFI 712 ACCGGT [GA]^CCGG[CT] BsrGI 995 TGTACA T^GTACA BsrI 341 CCAGT ACTGG(1/-1) BstEII 715 GGTAACC G^GT[ACGT]ACC BstPI 715 GGTAACC G^GT[ACGT]ACC Bsu36I 892 CCTCAGG CC^T[ACGT]AGG Cac8I 843 GCCTGC GC[ACGT]^[ACGT]GC CelII 1015 GCTGAGC GC^T[ACGT]AGC Cfr10I 712 ACCGGT [GA]^CCGG[CT] EaeI 58 TGGCCA [CT]^GGCC[GA] Eco81I 892 CCTCAGG CC^T[ACGT]AGG EcoO65I 715 GGTAACC G^GT[ACGT]ACC EcoRV 985 GATATC GAT^ATC HincII 529 GTCAAC GT[CT]^[GA]AC HindII 529 GTCAAC GT[CT]^[GA]AC MaeII 504 ACGT A^CGT MluNI 58 TGGCCA TGG^CCA MscI 58 TGGCCA TGG^CCA MseI 197 TTAA T^TAA NdeI 905 CATATG CA^TATG PaeR7I 633 CTCGAG C^TCGAG PinAI 712 ACCGGT A^CCGGT SacI 456 GAGCTC GAGCT^C SbfI 844 CCTGCAGG CCTGCA^GG SmlI 633 CTCGAG C^T[CT][GA]AG Sse8387I 844 CCTGCAGG CCTGCA^GG SspBI 995 TGTACA T^GTACA TaqI 634 TCGA T^CGA TfiI 918 GAATC G^A[AT]TC Tru9I 197 TTAA T^TAA TthHB8I 634 TCGA T^CGA XcmI 341 CCAGTGACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 633 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 471 GTAC GT^AC 996 GTAC Asp700I 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 665 GAAACCCTTC AspHI 223 GAGCAC G[AT]GC[AT]^C 456 GAGCTC AspI 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 435 GACCTTGTC BcnI 403 CCGGG CC^[GC]GG 452 CCCGG BseRI 775 CTCCTC GAGGAG(10/8) 778 CTCCTC BsiHKAI 223 GAGCAC G[AT]GC[AT]^C 456 GAGCTC BsmAI 127 GAGAC GTCTC(1/5) 238 GTCTC BstXI 410 CCACGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 616 CCATACGAGTGG DsaI 410 CCACGG C^C[GA][CT]GG 573 CCACGG EcoT14I 242 CCAAGG C^C[AT][AT]GG 524 CCAAGG MwoI 449 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 800 GCTGGTGCTGC NciI 403 CCGGG CC^[GC]GG 452 CCCGG NspI 467 ACATGT [GA]CATG^[CT] 732 GCATGT PflFI 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 435 GACCTTGTC RsaI 471 GTAC GT^AC 996 GTAC StyI 242 CCAAGG C^C[AT][AT]GG 524 CCAAGG Tth111I 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 435 GACCTTGTC XmnI 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 665 GAAACCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AluI ApaI AvaII BanII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboII MnlI MslI MspI MvaI NlaIII NlaIV PleI PpuMI PstI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI