(Circular) (Six-base) MAPSORT of: pFN21AB3452.seq Check: 6170 from: 1 to: 5356 pFN21AB3452 5356 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3166 278 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2699 1937 Size: 762 4594 Acc65I G'GTAC_C Cuts at: 2677 2677 Size: 5356 AclI AA'CG_TT Cuts at: 1563 3483 3856 1563 Size: 1920 373 3063 Fragments arranged by size: 3063 1920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3419 4803 5071 1632 Size: 1787 1384 268 1917 Fragments arranged by size: 1917 1787 1384 268 AfeI AGC'GCT Cuts at: 2147 2147 Size: 5356 AflII C'TTAA_G Cuts at: 820 1017 2168 820 Size: 197 1151 4008 Fragments arranged by size: 4008 1151 197 AflIII A'CryG_T Cuts at: 1203 4255 5232 1203 Size: 3052 977 1327 Fragments arranged by size: 3052 1327 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5356 AlwNI CAG_nnn'CTG Cuts at: 3099 4671 3099 Size: 1572 3784 ApaLI G'TGCA_C Cuts at: 3413 4569 3413 Size: 1156 4200 ApoI r'AATT_y Cuts at: 1493 2665 2891 3043 5152 1493 Size: 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 226 152 AseI AT'TA_AT Cuts at: 160 1032 3910 160 Size: 872 2878 1606 Fragments arranged by size: 2878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2152 2681 1870 Size: 73 209 529 4545 Fragments arranged by size: 4545 529 209 73 BamHI G'GATC_C Cuts at: 2686 3053 2686 Size: 367 4989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2677 4132 618 Size: 325 217 627 369 521 1455 1842 Fragments arranged by size: 1842 1455 627 521 369 325 217 BanII G_rGCy'C Cuts at: 1391 2675 1391 Size: 1284 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3547 3581 1903 Size: 34 1610 34 3678 Fragments arranged by size: 3678 1610 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3248 4458 3248 Size: 1210 4146 BclI T'GATC_A Cuts at: 1548 2352 1548 Size: 804 4552 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3967 136 Size: 107 122 71 631 605 2295 1525 Fragments arranged by size: 2295 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5351 5351 Size: 5356 BlpI GC'TnA_GC Cuts at: 2773 2773 Size: 5356 Bme1580I G_kGCm'C Cuts at: 1790 2159 3417 4573 1790 Size: 369 1258 1156 2573 Fragments arranged by size: 2573 1258 1156 369 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3087 4045 442 Size: 416 53 83 399 24 1670 958 1753 Fragments arranged by size: 1753 1670 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 4016 1239 Size: 872 1905 2579 Fragments arranged by size: 2579 1905 872 Bpu10I CC'TnA_GC Cuts at: 2235 2235 Size: 5356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2570 3481 4346 4644 2570 Size: 911 865 298 3282 Fragments arranged by size: 3282 911 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 882 Size: 617 2520 2219 Fragments arranged by size: 2520 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2518 3147 493 Size: 711 1314 629 2702 Fragments arranged by size: 2702 1314 711 629 BsaBI GATnn'nnATC Cuts at: 5350 5350 Size: 5356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3163 3545 4180 275 Size: 53 83 186 936 1630 382 635 1451 Fragments arranged by size: 1630 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3789 4187 4461 4608 5019 3789 Size: 398 274 147 411 4126 Fragments arranged by size: 4126 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5131 Fragments arranged by size: 5131 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5356 BseYI C'CCAG_C Cuts at: 2643 4559 5055 2643 Size: 1916 496 2944 Fragments arranged by size: 2944 1916 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 1163 Size: 573 306 4477 Fragments arranged by size: 4477 573 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2806 3567 4595 1540 Size: 275 186 805 761 1028 2301 Fragments arranged by size: 2301 1028 805 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2630 3417 3502 4573 2630 Size: 787 85 1071 3413 Fragments arranged by size: 3413 1071 787 85 BsmI GAATG_Cn' Cuts at: 2867 2960 2867 Size: 93 5263 BsmBI CGTCTCn'nnnn_ Cuts at: 2087 2087 Size: 5356 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2630 2675 3417 3502 4573 1391 Size: 399 369 471 45 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 399 369 85 45 BspEI T'CCGG_A Cuts at: 5019 5019 Size: 5356 BspHI T'CATG_A Cuts at: 3245 4975 3245 Size: 1730 3626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2711 844 Size: 1867 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3243 1394 Size: 429 1420 3507 Fragments arranged by size: 3507 1420 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2579 3851 4025 65 Size: 1183 1331 1272 174 1396 Fragments arranged by size: 1396 1331 1272 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4000 4187 1700 Size: 110 2190 187 2869 Fragments arranged by size: 2869 2190 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5356 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5356 BssSI C'ACGA_G Cuts at: 3416 4428 3416 Size: 1012 4344 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5356 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5356 BstYI r'GATC_y Cuts at: 1972 2686 3053 3438 3455 4907 4993 5005 5351 Size: 714 367 385 17 1452 86 12 346 Cuts at: 5351 1972 Size: 1977 Fragments arranged by size: 1977 1452 714 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2241 2631 513 Size: 552 1176 390 3238 Fragments arranged by size: 3238 1176 552 390 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4617 Fragments arranged by size: 4617 683 56 BtsI GCAGTG_nn' Cuts at: 2872 3666 3686 2872 Size: 794 20 4542 Fragments arranged by size: 4542 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2388 2660 2388 Size: 272 5084 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4363 809 Size: 507 3047 1802 Fragments arranged by size: 3047 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2313 2439 2803 Size: 54 1425 50 275 18 483 126 364 Cuts at: 2803 3692 5321 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 483 364 275 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2803 1537 Size: 275 991 4090 Fragments arranged by size: 4090 991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3286 1353 Size: 1933 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3943 4317 4463 387 Size: 1064 138 2354 374 146 1280 Fragments arranged by size: 2354 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 3419 4016 4803 5071 1239 Size: 393 479 1308 597 787 268 1524 Fragments arranged by size: 1524 1308 787 597 479 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2036 Size: 5356 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 EcoRV GAT'ATC Cuts at: 2248 2248 Size: 5356 FspI TGC'GCA Cuts at: 3862 3862 Size: 5356 HaeII r_GCGC'y Cuts at: 1678 2080 2149 2273 2650 4503 1678 Size: 402 69 124 377 1853 2531 Fragments arranged by size: 2531 1853 402 377 124 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 3997 4029 4071 Size: 32 135 32 872 32 1521 32 42 Cuts at: 4071 4103 1373 Size: 32 2626 Fragments arranged by size: 2626 1521 872 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2566 2700 2946 669 Size: 988 281 628 134 246 3079 Fragments arranged by size: 3079 988 628 281 246 134 HindIII A'AGCT_T Cuts at: 748 748 Size: 5356 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KpnI G_GTAC'C Cuts at: 2681 2681 Size: 5356 MfeI C'AATT_G Cuts at: 2955 2955 Size: 5356 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4470 4654 1305 Size: 3165 184 2007 Fragments arranged by size: 3165 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2315 2441 10 Size: 54 1425 343 483 126 2925 Fragments arranged by size: 2925 1425 483 343 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 3314 3673 3832 518 Size: 648 2148 359 159 2042 Fragments arranged by size: 2148 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2772 3449 4597 4842 1606 Size: 1166 677 1148 245 2120 Fragments arranged by size: 2120 1166 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2631 513 Size: 552 1566 3238 Fragments arranged by size: 3238 1566 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NruI TCG'CGA Cuts at: 2025 2025 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2714 4259 1132 Size: 97 1485 1545 2229 Fragments arranged by size: 2229 1545 1485 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5356 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5356 PfoI T'CCnGG_A Cuts at: 2088 2088 Size: 5356 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PmlI CAC'GTG Cuts at: 2518 2518 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 4938 4970 3425 Size: 32 1481 32 3811 Fragments arranged by size: 3811 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5356 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PstI C_TGCA'G Cuts at: 830 2708 830 Size: 1878 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SbfI CC_TGCA'GG Cuts at: 2708 2708 Size: 5356 ScaI AGT'ACT Cuts at: 3604 5316 3604 Size: 1712 3644 SfcI C'TryA_G Cuts at: 826 1045 2502 2704 3839 4520 4711 826 Size: 219 1457 202 1135 681 191 1471 Fragments arranged by size: 1471 1457 1135 681 219 202 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5356 SmaI CCC'GGG Cuts at: 1872 2683 1872 Size: 811 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 2585 3460 4361 4623 820 Size: 197 926 225 417 875 901 262 1553 Fragments arranged by size: 1553 926 901 875 417 262 225 197 SnaBI TAC'GTA Cuts at: 493 3147 493 Size: 2654 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2714 1132 Size: 1582 3774 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5356 StyI C'CwwG_G Cuts at: 513 1065 2631 2795 513 Size: 552 1566 164 3074 Fragments arranged by size: 3074 1566 552 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3383 3400 3553 3738 4183 325 Size: 1255 1803 17 153 185 445 1498 Fragments arranged by size: 1803 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3602 5314 96 Size: 274 80 33 51 1443 1625 1712 138 Fragments arranged by size: 1712 1625 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5316 5348 5316 Size: 32 5324 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5356 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5356 XmaI C'CCGG_G Cuts at: 1870 2681 1870 Size: 811 4545 XmnI GAAnn'nnTTC Cuts at: 3485 3485 Size: 5356 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3164 276 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI