################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tru9I TseI TspRI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcsI 320 GAATTT [GA]^AATT[CT] AfaI 25 GTAC GT^AC AluI 399 AGCT AG^CT Alw44I 97 GTGCAC G^TGCAC AlwI 83 GATCC GGATC(4/5) ApaLI 97 GTGCAC G^TGCAC ApoI 320 GAATTT [GA]^AATT[CT] AspHI 97 GTGCAC G[AT]GC[AT]^C AspI 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 123 GGACC G^G[AT]CC BbsI 8 GAAGAC GAAGAC(2/6) BfaI 175 CTAG C^TAG BmyI 97 GTGCAC G[AGT]GC[ACT]^C BpuAI 8 GAAGAC GAAGAC(2/6) BsaI 314 GGTCTC GGTCTC(1/5) BsiHKAI 97 GTGCAC G[AT]GC[AT]^C BsmBI 284 GAGACG CGTCTC(1/5) Bsp1286I 97 GTGCAC G[AGT]GC[ACT]^C BssKI 274 CCAGG ^CC[ACGT]GG BstBI 5 TTCGAA TT^CGAA BstNI 274 CCAGG CC^[AT]GG BstYI 82 AGATCC [GA]^GATC[CT] Cfr13I 123 GGACC G^G[ACGT]CC DraII 122 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 271 CGGCCA [CT]^GGCC[GA] EcoO109I 122 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRII 274 CCAGG ^CC[AT]GG HapII 270 CCGG C^CGG HpaII 270 CCGG C^CGG MaeI 175 CTAG C^TAG MaeII 287 ACGT A^CGT MboII 8 GAAGA GAAGA(8/7) MflI 82 AGATCC [GA]^GATC[CT] MspI 270 CCGG C^CGG MvaI 274 CCAGG CC^[AT]GG NlaIV 123 GGACCC GG[ACGT]^[ACGT]CC NspV 5 TTCGAA TT^CGAA PflFI 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 49 GAGTC GAGTC(4/5) PpuMI 122 AGGACCC [GA]G^G[AT]CC[CT] PshAI 44 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 25 GTAC GT^AC Sau96I 123 GGACC G^G[ACGT]CC ScrFI 274 CCAGG CC^[ACGT]GG SfuI 5 TTCGAA TT^CGAA SmlI 132 CTTGAG C^T[CT][GA]AG StuI 276 AGGCCT AGG^CCT TfiI 90 GAATC G^A[AT]TC Tsp509I 321 AATT ^AATT Tth111I 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 82 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### DdeI 280 CTCAG C^T[ACGT]AG 418 CTGAG DpnII 83 GATC ^GATC 197 GATC HaeIII 272 GGCC GG^CC 277 GGCC HinfI 49 GAGTC G^A[ACGT]TC 90 GAATC HphI 226 GGTGA GGTGA(8/7) 349 GGTGA MaeIII 160 GTCAC ^GT[ACGT]AC 422 GTGAC MboI 83 GATC ^GATC 197 GATC MnlI 279 CCTC CCTC(7/6) 375 GAGG NdeII 83 GATC ^GATC 197 GATC Sau3AI 83 GATC ^GATC 197 GATC SspI 165 AATATT AAT^ATT 406 AATATT TaqI 6 TCGA T^CGA 318 TCGA Tsp45I 160 GTCAC ^GT[GC]AC 422 GTGAC TthHB8I 6 TCGA T^CGA 318 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### BsmAI BsrI NlaIII