Restriction map for
pFN21AB0475
(Linear) (Six-base) MAPSORT of: KIBB0475.seq Check: 5014 from: 1 to: 1551
KIBB0475.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 520 811 1551
Size: 520 291 740
Fragments arranged by size:
740 520 291
AccI GT'mk_AC
Cuts at: 0 362 1551
Size: 362 1189
Acc65I G'GTAC_C
Cuts at: 0 454 773 1551
Size: 454 319 778
Fragments arranged by size:
778 454 319
AflIII A'CryG_T
Cuts at: 0 1181 1551
Size: 1181 370
AleI CACnn'nnGTG
Cuts at: 0 136 1551
Size: 136 1415
AlwNI CAG_nnn'CTG
Cuts at: 0 832 1245 1551
Size: 832 413 306
Fragments arranged by size:
832 413 306
ApoI r'AATT_y
Cuts at: 0 292 1003 1551
Size: 292 711 548
Fragments arranged by size:
711 548 292
AscI GG'CGCG_CC
Cuts at: 0 1041 1551
Size: 1041 510
BanI G'GyrC_C
Cuts at: 0 22 454 639 773 1053 1139 1551
Size: 22 432 185 134 280 86 412
Fragments arranged by size:
432 412 280 185 134 86 22
BbeI G_GCGC'C
Cuts at: 0 26 1143 1551
Size: 26 1117 408
Fragments arranged by size:
1117 408 26
BbvCI CC'TCA_GC
Cuts at: 0 519 1551
Size: 519 1032
BciVI GTATCCnnnnn_n'
Cuts at: 0 558 1551
Size: 558 993
Bme1580I G_kGCm'C
Cuts at: 0 644 1058 1551
Size: 644 414 493
Fragments arranged by size:
644 493 414
BmgBI CAC'GTC
Cuts at: 0 138 792 1551
Size: 138 654 759
Fragments arranged by size:
759 654 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 533 1300 1551
Size: 533 767 251
Fragments arranged by size:
767 533 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 367 1551
Size: 361 6 1184
Fragments arranged by size:
1184 361 6
Bpu10I CC'TnA_GC
Cuts at: 0 519 975 1475 1551
Size: 519 456 500 76
Fragments arranged by size:
519 500 456 76
BsaAI yAC'GTr
Cuts at: 0 1182 1548 1551
Size: 1182 366 3
Fragments arranged by size:
1182 366 3
BsaHI Gr'CG_yC
Cuts at: 0 23 80 1140 1388 1551
Size: 23 57 1060 248 163
Fragments arranged by size:
1060 248 163 57 23
BsaWI w'CCGG_w
Cuts at: 0 593 1448 1551
Size: 593 855 103
Fragments arranged by size:
855 593 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1005 1035 1551
Size: 1005 30 516
Fragments arranged by size:
1005 516 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1013 1324 1327 1330 1333 1336 1339 1342
Size: 1013 311 3 3 3 3 3 3
Cuts at: 1342 1551
Size: 209
Fragments arranged by size:
1013 311 209 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 172 643 825 1217 1551
Size: 172 471 182 392 334
Fragments arranged by size:
471 392 334 182 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 498 748 1551
Size: 130 368 250 803
Fragments arranged by size:
803 368 250 130
BsmI GAATG_Cn'
Cuts at: 0 64 1551
Size: 64 1487
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1025 1551
Size: 1025 526
Bsp1286I G_dGCh'C
Cuts at: 0 644 1058 1551
Size: 644 414 493
Fragments arranged by size:
644 493 414
BspEI T'CCGG_A
Cuts at: 0 593 1551
Size: 593 958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 520 785 811 1253 1551
Size: 520 265 26 442 298
Fragments arranged by size:
520 442 298 265 26
BsrFI r'CCGG_y
Cuts at: 0 1050 1142 1282 1551
Size: 1050 92 140 269
Fragments arranged by size:
1050 269 140 92
BssHII G'CGCG_C
Cuts at: 0 1041 1551
Size: 1041 510
BstEII G'GTnAC_C
Cuts at: 0 744 1551
Size: 744 807
BstXI CCAn_nnnn'nTGG
Cuts at: 0 876 1551
Size: 876 675
BstYI r'GATC_y
Cuts at: 0 590 1551
Size: 590 961
Bsu36I CC'TnA_GG
Cuts at: 0 740 1551
Size: 740 811
BtgI C'CryG_G
Cuts at: 0 251 1058 1409 1551
Size: 251 807 351 142
Fragments arranged by size:
807 351 251 142
BtsI GCAGTG_nn'
Cuts at: 0 147 470 1551
Size: 147 323 1081
Fragments arranged by size:
1081 323 147
DraIII CAC_nnn'GTG
Cuts at: 0 1294 1551
Size: 1294 257
EaeI y'GGCC_r
Cuts at: 0 233 428 506 1061 1551
Size: 233 195 78 555 490
Fragments arranged by size:
555 490 233 195 78
EarI CTCTTCn'nnn_
Cuts at: 0 904 1551
Size: 904 647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 316 1551
Size: 316 1235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 367 1551
Size: 361 6 1184
Fragments arranged by size:
1184 361 6
EcoO109I rG'GnC_Cy
Cuts at: 0 498 910 935 1551
Size: 498 412 25 616
Fragments arranged by size:
616 498 412 25
EcoRI G'AATT_C
Cuts at: 0 292 1003 1551
Size: 292 711 548
Fragments arranged by size:
711 548 292
FseI GG_CCGG'CC
Cuts at: 0 1286 1551
Size: 1286 265
HaeII r_GCGC'y
Cuts at: 0 26 1143 1551
Size: 26 1117 408
Fragments arranged by size:
1117 408 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 1551
Size: 35 32 1484
Fragments arranged by size:
1484 35 32
HincII GTy'rAC
Cuts at: 0 684 1551
Size: 684 867
KasI G'GCGC_C
Cuts at: 0 22 1139 1551
Size: 22 1117 412
Fragments arranged by size:
1117 412 22
KpnI G_GTAC'C
Cuts at: 0 458 777 1551
Size: 458 319 774
Fragments arranged by size:
774 458 319
MfeI C'AATT_G
Cuts at: 0 441 1551
Size: 441 1110
MscI TGG'CCA
Cuts at: 0 235 430 508 1551
Size: 235 195 78 1043
Fragments arranged by size:
1043 235 195 78
MslI CAynn'nnrTG
Cuts at: 0 136 256 424 1184 1551
Size: 136 120 168 760 367
Fragments arranged by size:
760 367 168 136 120
MspA1I CmG'CkG
Cuts at: 0 28 783 1482 1551
Size: 28 755 699 69
Fragments arranged by size:
755 699 69 28
NaeI GCC'GGC
Cuts at: 0 1144 1284 1551
Size: 1144 140 267
Fragments arranged by size:
1144 267 140
NarI GG'CG_CC
Cuts at: 0 23 1140 1551
Size: 23 1117 411
Fragments arranged by size:
1117 411 23
NcoI C'CATG_G
Cuts at: 0 251 1551
Size: 251 1300
NgoMIV G'CCGG_C
Cuts at: 0 1142 1282 1551
Size: 1142 140 269
Fragments arranged by size:
1142 269 140
PflMI CCAn_nnn'nTGG
Cuts at: 0 443 1294 1551
Size: 443 851 257
Fragments arranged by size:
851 443 257
PmlI CAC'GTG
Cuts at: 0 1182 1551
Size: 1182 369
PpuMI rG'GwC_Cy
Cuts at: 0 498 910 935 1551
Size: 498 412 25 616
Fragments arranged by size:
616 498 412 25
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1234 1266 1551
Size: 1234 32 285
Fragments arranged by size:
1234 285 32
PstI C_TGCA'G
Cuts at: 0 154 782 1250 1551
Size: 154 628 468 301
Fragments arranged by size:
628 468 301 154
PvuII CAG'CTG
Cuts at: 0 1482 1551
Size: 1482 69
SanDI GG'GwC_CC
Cuts at: 0 910 1551
Size: 910 641
SfcI C'TryA_G
Cuts at: 0 150 778 921 1164 1197 1246 1551
Size: 150 628 143 243 33 49 305
Fragments arranged by size:
628 305 243 150 143 49 33
SfoI GGC'GCC
Cuts at: 0 24 1141 1551
Size: 24 1117 410
Fragments arranged by size:
1117 410 24
StyI C'CwwG_G
Cuts at: 0 251 668 869 1551
Size: 251 417 201 682
Fragments arranged by size:
682 417 251 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 1241 1282 1551
Size: 202 1039 41 269
Fragments arranged by size:
1039 269 202 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 649 681 1005 1037 1551
Size: 649 32 324 32 514
Fragments arranged by size:
649 514 324 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 496 1551
Size: 496 1055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 228 1551
Size: 228 1323
Enzymes that do cut:
AarI AccI Acc65I AflIII AleI AlwNI ApoI AscI
BanI BbeI BbvCI BciVI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsmI BsmBI Bsp1286I BspEI BspMI BsrFI BssHII BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoO109I EcoRI FseI HaeII Hin4I HincII
KasI KpnI MfeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PflMI PmlI PpuMI PsrI PstI PvuII
SanDI SfcI SfoI StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbsI BcgI BclI BfrBI BglI BglII
BlpI BmtI BplI BpuEI BsaI BsaBI BsiEI BsiHKAI
BsiWI BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoNI EcoRV FalI FspI FspAI HindIII HpaI MluI
MmeI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI XbaI
XhoI XmaI XmnI ZraI