Restriction map for
pFN21AB5440
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AlwNI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BbeI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI HphI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tsp509I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AhdI 43 GACGGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 969 GTGCAC G^TGCAC
AlwI 369 GATCC GGATC(4/5)
AocI 532 CCTGAGG CC^T[ACGT]AGG
Aor51HI 426 AGCGCT AGC^GCT
ApaLI 969 GTGCAC G^TGCAC
AspEI 43 GACGGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 550 CACGTG CAC^GTG
BlpI 150 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 150 GCTCAGC GC^T[ACGT]AGC
BsaI 199 GAGACC GGTCTC(1/5)
BsgI 918 CTGCAC GTGCAG(16/14)
BsmAI 199 GAGAC GTCTC(1/5)
BstYI 368 AGATCC [GA]^GATC[CT]
Bsu36I 532 CCTGAGG CC^T[ACGT]AGG
CelII 150 GCTCAGC GC^T[ACGT]AGC
DpnII 369 GATC ^GATC
Eam1105I 43 GACGGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 792 GAAGAG CTCTTC(1/4)
Eco47III 426 AGCGCT AGC^GCT
Eco81I 532 CCTGAGG CC^T[ACGT]AGG
EcoNI 8 CCTCGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 426 AGCGCT [GA]GCGC^[CT]
HgaI 259 GACGC GACGC(5/10)
HincII 490 GTTGAC GT[CT]^[GA]AC
HindII 490 GTTGAC GT[CT]^[GA]AC
HinfI 740 GAATC G^A[ACGT]TC
Ksp632I 792 GAAGAG CTCTTC(1/4)
MaeIII 947 GTGAC ^GT[ACGT]AC
MboI 369 GATC ^GATC
MflI 368 AGATCC [GA]^GATC[CT]
MseI 704 TTAA T^TAA
NdeII 369 GATC ^GATC
NspI 341 ACATGC [GA]CATG^[CT]
PmaCI 550 CACGTG CAC^GTG
PmlI 550 CACGTG CAC^GTG
PshAI 591 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 145 CTGCAG CTGCA^G
Sau3AI 369 GATC ^GATC
SexAI 395 ACCAGGT A^CC[AT]GGT
SfcI 145 CTGCAG C^T[GA][CT]AG
SmaI 173 CCCGGG CCC^GGG
StuI 855 AGGCCT AGG^CCT
TaqI 314 TCGA T^CGA
TfiI 740 GAATC G^A[AT]TC
Tru9I 704 TTAA T^TAA
Tsp45I 947 GTGAC ^GT[GC]AC
TthHB8I 314 TCGA T^CGA
XhoII 368 AGATCC [GA]^GATC[CT]
XmaCI 173 CCCGGG C^CCGGG
XmaI 173 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 753 GTAC GT^AC
873 GTAC
BanI 294 GGTGCC G^G[CT][GA]CC
374 GGCACC
BpmI 718 CTCCAG CTGGAG(16/14)
448 CTGGAG
BsaAI 385 TACGTG [CT]AC^GT[GA]
550 CACGTG
BseRI 316 GAGGAG GAGGAG(10/8)
511 GAGGAG
BsrDI 474 CATTGC GCAATG(2/0)
641 GCAATG
BsrFI 632 ACCGGC [GA]^CCGG[CT]
695 ACCGGC
BsrI 248 ACTGG ACTGG(1/-1)
748 ACTGG
Cac8I 109 GCCTGC GC[ACGT]^[ACGT]GC
220 GCCTGC
Cfr10I 632 ACCGGC [GA]^CCGG[CT]
695 ACCGGC
FauI 887 CCCGC CCCGC(4/6)
908 GCGGG
MspA1I 28 CAGCTG C[AC]G^C[GT]G
153 CAGCTG
PflMI 276 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
959 CCACACCCTGG
PvuII 28 CAGCTG CAG^CTG
153 CAGCTG
RsaI 753 GTAC GT^AC
873 GTAC
SacI 319 GAGCTC GAGCT^C
565 GAGCTC
Van91I 276 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
959 CCACACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BssKI BstF5I BstNI BstUI
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeII
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI TspRI