Restriction map for
pFN21AB6125
(Circular) (Six-base) MAPSORT of: pFN21AB6125.seq Check: 506 from: 1 to: 6463
pFN21AB6125 6463 bp
With 182 enzymes: SgfI *
May 6, 2010 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2182 2182
Size: 6463
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4273 278
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3806 1937
Size: 1869 4594
Acc65I G'GTAC_C
Cuts at: 3784 3784
Size: 6463
AclI AA'CG_TT
Cuts at: 1563 4590 4963 1563
Size: 3027 373 3063
Fragments arranged by size:
3063 3027 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2522 3494 3678 4526 5910 6178 1632
Size: 890 972 184 848 1384 268 1917
Fragments arranged by size:
1917 1384 972 890 848 268 184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6266
AflIII A'CryG_T
Cuts at: 1203 5362 6339 1203
Size: 4159 977 1327
Fragments arranged by size:
4159 1327 977
AgeI A'CCGG_T
Cuts at: 5294 5294
Size: 6463
AhdI GACnn_n'nnGTC
Cuts at: 5192 5192
Size: 6463
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2781 1184
Size: 1597 4866
AlwNI CAG_nnn'CTG
Cuts at: 3320 4206 5778 3320
Size: 886 1572 4005
Fragments arranged by size:
4005 1572 886
ApaLI G'TGCA_C
Cuts at: 4520 5676 4520
Size: 1156 5307
ApoI r'AATT_y
Cuts at: 1493 2431 3359 3601 3772 3998 4150 6259 1493
Size: 938 928 242 171 226 152 2109 1697
Fragments arranged by size:
2109 1697 938 928 242 226 171 152
AseI AT'TA_AT
Cuts at: 160 1032 5017 160
Size: 872 3985 1606
Fragments arranged by size:
3985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
AvaI C'yCGr_G
Cuts at: 1870 1943 3207 3788 1870
Size: 73 1264 581 4545
Fragments arranged by size:
4545 1264 581 73
BamHI G'GATC_C
Cuts at: 3793 4160 3793
Size: 367 6096
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2393 3784 5239 618
Size: 325 217 627 606 1391 1455 1842
Fragments arranged by size:
1842 1455 1391 627 606 325 217
BanII G_rGCy'C
Cuts at: 1391 2075 2121 2229 3782 1391
Size: 684 46 108 1553 4072
Fragments arranged by size:
4072 1553 684 108 46
BbeI G_GCGC'C
Cuts at: 2397 2397
Size: 6463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5492
BbvCI CC'TCA_GC
Cuts at: 3313 3313
Size: 6463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2005 2039 2415 2449 4654 4688 1903
Size: 34 68 34 376 34 2205 34 3678
Fragments arranged by size:
3678 2205 376 68 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3214 4355 5565 3214
Size: 1141 1210 4112
Fragments arranged by size:
4112 1210 1141
BclI T'GATC_A
Cuts at: 1548 3417 1548
Size: 1869 4594
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 5074 136
Size: 107 122 71 631 605 377 3025 1525
Fragments arranged by size:
3025 1525 631 605 377 122 107 71
BglII A'GATC_T
Cuts at: 6458 6458
Size: 6463
BlpI GC'TnA_GC
Cuts at: 2069 3880 2069
Size: 1811 4652
Bme1580I G_kGCm'C
Cuts at: 1790 3572 4524 5680 1790
Size: 1782 952 1156 2573
Fragments arranged by size:
2573 1782 1156 952
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6463
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2512 4194 5152
Size: 416 53 83 399 24 1095 1682 958
Cuts at: 5152 442
Size: 1753
Fragments arranged by size:
1753 1682 1095 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2183 2816 3708 5123 1239
Size: 839 105 633 892 1415 2579
Fragments arranged by size:
2579 1415 892 839 633 105
Bpu10I CC'TnA_GC
Cuts at: 2533 3313 2533
Size: 780 5683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2222 2840 3202 3445 4588 5453 5751 2222
Size: 618 362 243 1143 865 298 2934
Fragments arranged by size:
2934 1143 865 618 362 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2246 2651 5126 882
Size: 617 747 405 2475 2219
Fragments arranged by size:
2475 2219 747 617 405
BsaAI yAC'GTr
Cuts at: 493 1204 3233 4254 493
Size: 711 2029 1021 2702
Fragments arranged by size:
2702 2029 1021 711
BsaBI GATnn'nnATC
Cuts at: 6457 6457
Size: 6463
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2394 2704 4270 4652
Size: 53 83 186 936 861 310 1566 382
Cuts at: 4652 5287 275
Size: 635 1451
Fragments arranged by size:
1566 1451 936 861 635 382 310 186
83 53
BsaWI w'CCGG_w
Cuts at: 4896 5294 5568 5715 6126 4896
Size: 398 274 147 411 5233
Fragments arranged by size:
5233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3603 3633 3681 3711 1178
Size: 30 165 30 2200 30 48 30 3930
Fragments arranged by size:
3930 2200 165 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2222 2348 2660 2804 3629 3680 3683
Size: 934 102 126 312 144 825 51 3
Cuts at: 3683 3686 1186
Size: 3 3963
Fragments arranged by size:
3963 934 825 312 144 126 102 51
3 3
BseYI C'CCAG_C
Cuts at: 2323 2919 3408 5666 6162 2323
Size: 596 489 2258 496 2624
Fragments arranged by size:
2624 2258 596 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 1163
Size: 573 706 5184
Fragments arranged by size:
5184 706 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2221 3913 4674 5702 1540
Size: 275 186 220 1692 761 1028 2301
Fragments arranged by size:
2301 1692 1028 761 275 220 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2229 4524 4609 5680 2121
Size: 108 2295 85 1071 2904
Fragments arranged by size:
2904 2295 1071 108 85
BsmI GAATG_Cn'
Cuts at: 2830 3974 4067 2830
Size: 1144 93 5226
Fragments arranged by size:
5226 1144 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2159 2159
Size: 6463
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2121 2229 3572 3782 4524 4609
Size: 399 285 46 108 1343 210 742 85
Cuts at: 4609 5680 1391
Size: 1071 2174
Fragments arranged by size:
2174 1343 1071 742 399 285 210 108
85 46
BspEI T'CCGG_A
Cuts at: 6126 6126
Size: 6463
BspHI T'CATG_A
Cuts at: 2743 4352 6082 2743
Size: 1609 1730 3124
Fragments arranged by size:
3124 1730 1609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2182 3818 844
Size: 1338 1636 3489
Fragments arranged by size:
3489 1636 1338
BsrBI CCG'CTC
Cuts at: 1394 1823 4350 1394
Size: 429 2527 3507
Fragments arranged by size:
3507 2527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4958 5132 65
Size: 1183 3710 174 1396
Fragments arranged by size:
3710 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2127 2231 5107 5294 1700
Size: 110 317 104 2876 187 2869
Fragments arranged by size:
2876 2869 317 187 110 104
BsrGI T'GTAC_A
Cuts at: 96 2139 96
Size: 2043 4420
BssHII G'CGCG_C
Cuts at: 1925 2178 2277 2302 1925
Size: 253 99 25 6086
Fragments arranged by size:
6086 253 99 25
BssSI C'ACGA_G
Cuts at: 3562 4523 5535 3562
Size: 961 1012 4490
Fragments arranged by size:
4490 1012 961
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2450 794
Size: 1656 4807
BstBI TT'CG_AA
Cuts at: 2435 5267 2435
Size: 2832 3631
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6463
BstYI r'GATC_y
Cuts at: 1972 2497 3793 4160 4545 4562 6014 6100 6112
Size: 525 1296 367 385 17 1452 86 12
Cuts at: 6112 6458 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1296 525 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2030 513
Size: 552 939 26 4946
Fragments arranged by size:
4946 939 552 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5724
Fragments arranged by size:
5724 683 56
BtsI GCAGTG_nn'
Cuts at: 3979 4773 4793 3979
Size: 794 20 5649
Fragments arranged by size:
5649 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6428
DraI TTT'AAA
Cuts at: 3643 3767 3643
Size: 124 6339
DraIII CAC_nnn'GTG
Cuts at: 2465 2465
Size: 6463
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3440 5470 809
Size: 507 2124 2030 1802
Fragments arranged by size:
2124 2030 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2218 2262 3910
Size: 54 1425 50 275 18 388 44 1648
Cuts at: 3910 4799 6428 8
Size: 889 1629 43
Fragments arranged by size:
1648 1629 1425 889 388 275 54 50
44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2218 3910 1537
Size: 275 406 1692 4090
Fragments arranged by size:
4090 1692 406 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2637 3153 3248 3657 4393 1353
Size: 1284 516 95 409 736 3423
Fragments arranged by size:
3423 1284 736 516 409 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2354 3059 3298 5050 5424 5570
Size: 1064 138 765 705 239 1752 374 146
Cuts at: 5570 387
Size: 1280
Fragments arranged by size:
1752 1280 1064 765 705 374 239 146
138
EcoICRI GAG'CTC
Cuts at: 2119 2227 2119
Size: 108 6355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2183 2522 2816 3494 3678 3708
Size: 393 446 105 339 294 678 184 30
Cuts at: 3708 4526 5123 5910 6178 1239
Size: 818 597 787 268 1524
Fragments arranged by size:
1524 818 787 678 597 446 393 339
294 268 184 105 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2609 2609
Size: 6463
EcoRI G'AATT_C
Cuts at: 2431 3772 4150 2431
Size: 1341 378 4744
Fragments arranged by size:
4744 1341 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3204 3204
Size: 6463
FspI TGC'GCA
Cuts at: 2156 4969 2156
Size: 2813 3650
HaeII r_GCGC'y
Cuts at: 1678 2246 2397 5610 1678
Size: 568 151 3213 2531
Fragments arranged by size:
3213 2531 568 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2265 2297 5104 5136 5178
Size: 32 135 32 693 32 2807 32 42
Cuts at: 5178 5210 1373
Size: 32 2626
Fragments arranged by size:
2807 2626 693 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2863 3807 4053 669
Size: 988 281 925 944 246 3079
Fragments arranged by size:
3079 988 944 925 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 748
Size: 1657 4806
HpaI GTT'AAC
Cuts at: 4053 4053
Size: 6463
KasI G'GCGC_C
Cuts at: 2393 2393
Size: 6463
KpnI G_GTAC'C
Cuts at: 3788 3788
Size: 6463
MfeI C'AATT_G
Cuts at: 4062 4062
Size: 6463
MluI A'CGCG_T
Cuts at: 6339 6339
Size: 6463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2502 5577 5761 1305
Size: 1197 3075 184 2007
Fragments arranged by size:
3075 2007 1197 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4641
Fragments arranged by size:
4641 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4421 4780 4939 518
Size: 648 3255 359 159 2042
Fragments arranged by size:
3255 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2068 2188 2353 3073 3686 3879 4556
Size: 426 36 120 165 720 613 193 677
Cuts at: 4556 5704 5949 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 720 677 613 426 245 193
165 120 36
NarI GG'CG_CC
Cuts at: 2394 2394
Size: 6463
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4972
Fragments arranged by size:
4972 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6463
NotI GC'GGCC_GC
Cuts at: 3910 3910
Size: 6463
NspI r_CATG'y
Cuts at: 1132 1229 2638 2955 3019 3821 5366 1132
Size: 97 1409 317 64 802 1545 2229
Fragments arranged by size:
2229 1545 1409 802 317 97 64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6463
PciI A'CATG_T
Cuts at: 5362 5362
Size: 6463
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6463
PfoI T'CCnGG_A
Cuts at: 2094 2094
Size: 6463
PmeI GTTT'AAAC
Cuts at: 3767 3767
Size: 6463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 4532 4564 6045 6077 3545
Size: 32 955 32 1481 32 3931
Fragments arranged by size:
3931 1481 955 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6463
PsiI TTA'TAA
Cuts at: 3450 4033 3450
Size: 583 5880
PstI C_TGCA'G
Cuts at: 830 2187 2193 2316 2542 3815 830
Size: 1357 6 123 226 1273 3478
Fragments arranged by size:
3478 1357 1273 226 123 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6463
PvuII CAG'CTG
Cuts at: 2188 2353 3073 3686 2188
Size: 165 720 613 4965
Fragments arranged by size:
4965 720 613 165
SacI G_AGCT'C
Cuts at: 2121 2229 2121
Size: 108 6355
SacII CC_GC'GG
Cuts at: 2033 2033
Size: 6463
SalI G'TCGA_C
Cuts at: 1936 3805 1936
Size: 1869 4594
SbfI CC_TGCA'GG
Cuts at: 3815 3815
Size: 6463
ScaI AGT'ACT
Cuts at: 4711 6423 4711
Size: 1712 4751
SfcI C'TryA_G
Cuts at: 826 1045 2183 2189 2312 2538 2933 3811 4946
Size: 219 1138 6 123 226 395 878 1135
Cuts at: 4946 5627 5818 826
Size: 681 191 1471
Fragments arranged by size:
1471 1138 1135 878 681 395 226 219
191 123 6
SfoI GGC'GCC
Cuts at: 2395 2395
Size: 6463
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6463
SmaI CCC'GGG
Cuts at: 1872 3790 1872
Size: 1918 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2237 2819 3217 3460 4567 5468
Size: 197 926 294 582 398 243 1107 901
Cuts at: 5468 5730 820
Size: 262 1553
Fragments arranged by size:
1553 1107 926 901 582 398 294 262
243 197
SnaBI TAC'GTA
Cuts at: 493 3233 4254 493
Size: 2740 1021 2702
Fragments arranged by size:
2740 2702 1021
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6463
SphI G_CATG'C
Cuts at: 1132 3019 3821 1132
Size: 1887 802 3774
Fragments arranged by size:
3774 1887 802
SspI AAT'ATT
Cuts at: 5 52 4387 5
Size: 47 4335 2081
Fragments arranged by size:
4335 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6463
StyI C'CwwG_G
Cuts at: 513 1065 2004 3902 513
Size: 552 939 1898 3074
Fragments arranged by size:
3074 1898 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 4490 4507 4660 4845 5290 325
Size: 1255 690 2220 17 153 185 445 1498
Fragments arranged by size:
2220 1498 1255 690 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 2721 3632
Size: 274 80 33 51 1443 162 582 911
Cuts at: 3632 4709 6421 96
Size: 1077 1712 138
Fragments arranged by size:
1712 1443 1077 911 582 274 162 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6423 6455 6423
Size: 32 6431
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6463
XbaI T'CTAG_A
Cuts at: 2945 3799 2945
Size: 854 5609
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3405 3405
Size: 6463
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6463
XmaI C'CCGG_G
Cuts at: 1870 3788 1870
Size: 1918 4545
XmnI GAAnn'nnTTC
Cuts at: 2964 3090 4592 2964
Size: 126 1502 4835
Fragments arranged by size:
4835 1502 126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4271 276
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI
SwaI