(Circular) (Six-base) MAPSORT of: pFN21AB9000.seq Check: 2272 from: 1 to: 5959 pFN21AB9000 5959 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2386 2400 2386 Size: 14 5945 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2255 3769 278 Size: 53 83 186 936 719 1514 2468 Fragments arranged by size: 2468 1514 936 719 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 2702 3280 2702 Size: 578 5381 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4022 5406 5674 1632 Size: 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 3048 4858 5835 1203 Size: 1845 1810 977 1327 Fragments arranged by size: 1845 1810 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2237 1184 Size: 1053 4906 AlwNI CAG_nnn'CTG Cuts at: 2320 3702 5274 2320 Size: 1382 1572 3005 Fragments arranged by size: 3005 1572 1382 ApaI G_GGCC'C Cuts at: 2037 2037 Size: 5959 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 3119 3239 3268 3494 3646 5755 1493 Size: 1626 120 29 226 152 2109 1697 Fragments arranged by size: 2109 1697 1626 226 152 120 29 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2108 2853 3284 1870 Size: 73 95 70 745 431 4545 Fragments arranged by size: 4545 745 431 95 73 70 BamHI G'GATC_C Cuts at: 2953 3289 3656 2953 Size: 336 367 5256 Fragments arranged by size: 5256 367 336 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2683 2702 2917 3280 4735 Size: 325 217 627 896 19 215 363 1455 Cuts at: 4735 618 Size: 1842 Fragments arranged by size: 1842 1455 896 627 363 325 217 215 19 BanII G_rGCy'C Cuts at: 1391 2037 3278 1391 Size: 646 1241 4072 Fragments arranged by size: 4072 1241 646 BbeI G_GCGC'C Cuts at: 2687 2921 2687 Size: 234 5725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2560 2759 3160 928 Size: 971 661 199 401 3727 Fragments arranged by size: 3727 971 661 401 199 BbvCI CC'TCA_GC Cuts at: 2282 2282 Size: 5959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2119 2153 4150 4184 1903 Size: 34 50 34 98 34 1997 34 3678 Fragments arranged by size: 3678 1997 98 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2177 2297 4570 Size: 107 122 71 631 605 505 120 2273 Cuts at: 4570 136 Size: 1525 Fragments arranged by size: 2273 1525 631 605 505 122 120 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 2534 2546 3376 2534 Size: 12 830 5117 Fragments arranged by size: 5117 830 12 Bme1580I G_kGCm'C Cuts at: 1790 2037 4020 5176 1790 Size: 247 1983 1156 2573 Fragments arranged by size: 2573 1983 1156 247 BmgBI CAC'GTC Cuts at: 1318 2137 1318 Size: 819 5140 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3020 3690 4648 Size: 416 53 83 399 24 1603 670 958 Cuts at: 4648 442 Size: 1753 Fragments arranged by size: 1753 1603 958 670 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 2183 4619 1239 Size: 793 151 2436 2579 Fragments arranged by size: 2579 2436 793 151 Bpu10I CC'TnA_GC Cuts at: 2282 2282 Size: 5959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2459 4084 4949 5247 2459 Size: 1625 865 298 3171 Fragments arranged by size: 3171 1625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2653 3750 493 Size: 711 1449 1097 2702 Fragments arranged by size: 2702 1449 1097 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2240 2252 2684 2759 Size: 53 83 186 936 707 12 432 75 Cuts at: 2759 2918 3766 4148 4783 275 Size: 159 848 382 635 1451 Fragments arranged by size: 1451 936 848 707 635 432 382 186 159 83 75 53 12 BsaWI w'CCGG_w Cuts at: 2235 4392 4790 5064 5211 5622 2235 Size: 2157 398 274 147 411 2572 Fragments arranged by size: 2572 2157 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 1186 Size: 976 4983 BseYI C'CCAG_C Cuts at: 2203 2334 2613 2913 5162 5658 2203 Size: 131 279 300 2249 496 2504 Fragments arranged by size: 2504 2249 496 300 279 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 2799 1163 Size: 573 480 583 4323 Fragments arranged by size: 4323 583 573 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2449 2523 3409 4170 5198 1540 Size: 275 186 448 74 886 761 1028 2301 Fragments arranged by size: 2301 1028 886 761 448 275 186 74 BsiHKAI G_wGCw'C Cuts at: 2552 4020 4105 5176 2552 Size: 1468 85 1071 3335 Fragments arranged by size: 3335 1468 1071 85 BsmI GAATG_Cn' Cuts at: 2306 3470 3563 2306 Size: 1164 93 4702 Fragments arranged by size: 4702 1164 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2015 2015 Size: 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2552 3278 4020 4105 5176 1391 Size: 399 247 515 726 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 726 515 399 247 85 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2436 2511 3848 5578 2436 Size: 75 1337 1730 2817 Fragments arranged by size: 2817 1730 1337 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2386 2400 2714 3314 844 Size: 1542 14 314 600 3489 Fragments arranged by size: 3489 1542 600 314 14 BsrBI CCG'CTC Cuts at: 1394 1823 2473 2502 3846 1394 Size: 429 650 29 1344 3507 Fragments arranged by size: 3507 1344 650 429 29 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 2072 4603 4790 1700 Size: 110 208 54 2531 187 2869 Fragments arranged by size: 2869 2531 208 187 110 54 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 2495 1925 Size: 570 5389 BssSI C'ACGA_G Cuts at: 2643 4019 5031 2643 Size: 1376 1012 3571 Fragments arranged by size: 3571 1376 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2862 2953 3289 3656 4041 4058 5510 5596 Size: 890 91 336 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 890 385 367 346 336 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2054 2301 2356 2688 2746 513 Size: 552 989 247 55 332 58 3726 Fragments arranged by size: 3726 989 552 332 247 58 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2440 487 Size: 683 56 1214 4006 Fragments arranged by size: 4006 1214 683 56 BtsI GCAGTG_nn' Cuts at: 3000 3475 4269 4289 3000 Size: 475 794 20 4670 Fragments arranged by size: 4670 794 475 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DraIII CAC_nnn'GTG Cuts at: 2828 2828 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2154 2196 Size: 54 1425 50 275 18 237 87 42 Cuts at: 2196 2289 2298 2520 3061 3406 4295 5924 8 Size: 93 9 222 541 345 889 1629 43 Fragments arranged by size: 1629 1425 889 541 345 275 237 222 93 87 54 50 43 42 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2520 3406 1537 Size: 275 708 886 4090 Fragments arranged by size: 4090 886 708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2273 3889 1353 Size: 920 1616 3423 Fragments arranged by size: 3423 1616 920 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2005 4546 4920 5066 387 Size: 1064 138 416 2541 374 146 1280 Fragments arranged by size: 2541 1280 1064 416 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2183 4022 4619 5406 5674 1239 Size: 393 400 151 1839 597 787 268 1524 Fragments arranged by size: 1839 1524 787 597 400 393 268 151 EcoNI CCTnn'n_nnAGG Cuts at: 2111 2111 Size: 5959 EcoO109I rG'GnC_Cy Cuts at: 2033 2034 2033 Size: 1 5958 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3177 3177 Size: 5959 FspI TGC'GCA Cuts at: 2141 4465 2141 Size: 2324 3635 HaeII r_GCGC'y Cuts at: 1678 2079 2687 2921 5106 1678 Size: 401 608 234 2185 2531 Fragments arranged by size: 2531 2185 608 401 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2945 2977 4600 4632 4674 Size: 32 135 32 1373 32 1623 32 42 Cuts at: 4674 4706 1373 Size: 32 2626 Fragments arranged by size: 2626 1623 1373 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 3088 748 Size: 2340 3619 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2683 2917 2683 Size: 234 5725 KpnI G_GTAC'C Cuts at: 2706 3284 2706 Size: 578 5381 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2299 5073 5257 1305 Size: 994 2774 184 2007 Fragments arranged by size: 2774 2007 994 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2069 2156 3063 10 Size: 54 1425 343 237 87 907 2906 Fragments arranged by size: 2906 1425 907 343 237 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 2678 2936 3917 4276 4435 518 Size: 648 1293 219 258 981 359 159 2042 Fragments arranged by size: 2042 1293 981 648 359 258 219 159 MspA1I CmG'CkG Cuts at: 1606 2358 2748 3375 4052 5200 5445 1606 Size: 752 390 627 677 1148 245 2120 Fragments arranged by size: 2120 1148 752 677 627 390 245 NarI GG'CG_CC Cuts at: 2684 2918 2684 Size: 234 5725 NcoI C'CATG_G Cuts at: 513 1065 2054 513 Size: 552 989 4418 Fragments arranged by size: 4418 989 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 2520 3406 2520 Size: 886 5073 NspI r_CATG'y Cuts at: 1132 1229 2263 2410 2428 3052 3317 4862 1132 Size: 97 1034 147 18 624 265 1545 2229 Fragments arranged by size: 2229 1545 1034 624 265 147 97 18 PasI CC'CwG_GG Cuts at: 1348 2634 1348 Size: 1286 4673 PciI A'CATG_T Cuts at: 3048 4858 3048 Size: 1810 4149 PflMI CCAn_nnn'nTGG Cuts at: 1108 2055 1108 Size: 947 5012 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2033 2033 Size: 5959 PstI C_TGCA'G Cuts at: 830 2397 3311 830 Size: 1567 914 3478 Fragments arranged by size: 3478 1567 914 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SacII CC_GC'GG Cuts at: 2359 2749 2359 Size: 390 5569 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 2397 3311 2397 Size: 914 5045 ScaI AGT'ACT Cuts at: 2561 4207 5919 2561 Size: 1646 1712 2601 Fragments arranged by size: 2601 1712 1646 SfcI C'TryA_G Cuts at: 826 1045 2393 3307 4442 5123 5314 826 Size: 219 1348 914 1135 681 191 1471 Fragments arranged by size: 1471 1348 1135 914 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2297 2297 Size: 5959 SfoI GGC'GCC Cuts at: 2685 2919 2685 Size: 234 5725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 2072 1700 Size: 372 5587 SmaI CCC'GGG Cuts at: 1872 2855 3286 1872 Size: 983 431 4545 Fragments arranged by size: 4545 983 431 SmlI C'TyrA_G Cuts at: 820 1017 1943 2474 4063 4964 5226 820 Size: 197 926 531 1589 901 262 1553 Fragments arranged by size: 1589 1553 926 901 531 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 2263 3317 1132 Size: 1131 1054 3774 Fragments arranged by size: 3774 1131 1054 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2054 2598 3398 513 Size: 552 989 544 800 3074 Fragments arranged by size: 3074 989 800 552 544 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2518 2987 3986 4003 4156 4341 4786 Size: 1255 938 469 999 17 153 185 445 Cuts at: 4786 325 Size: 1498 Fragments arranged by size: 1498 1255 999 938 469 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2559 4205 5917 Size: 274 80 33 51 1443 582 1646 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1712 1646 1443 582 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 2758 1316 Size: 1442 4517 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 2853 3284 1870 Size: 983 431 4545 Fragments arranged by size: 4545 983 431 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2253 3767 276 Size: 53 83 186 936 719 1514 2468 Fragments arranged by size: 2468 1514 936 719 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI