Restriction map for pFN21AB9000
 (Circular) (Six-base) MAPSORT of: pFN21AB9000.seq  Check: 2272  from: 1  to: 5959



pFN21AB9000  5959 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2386    2400    2386 
   Size:         14    5945
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2255    3769     278 
   Size:         53      83     186     936     719    1514    2468

  Fragments arranged by size: 

                2468    1514     936     719     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3302    1937 
   Size:       1365    4594
 
Acc65I G'GTAC_C

Cuts at:   2702    3280    2702 
   Size:        578    5381
 
AclI AA'CG_TT

Cuts at:   1563    4086    4459    1563 
   Size:       2523     373    3063

  Fragments arranged by size: 

                3063    2523     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    4022    5406    5674    1632 
   Size:       2390    1384     268    1917

  Fragments arranged by size: 

                2390    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5762
 
AflIII A'CryG_T

Cuts at:   1203    3048    4858    5835    1203 
   Size:       1845    1810     977    1327

  Fragments arranged by size: 

                1845    1810    1327     977
 
AgeI A'CCGG_T

Cuts at:   4790    4790 
   Size:       5959
 
AhdI GACnn_n'nnGTC

Cuts at:   4688    4688 
   Size:       5959
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5959
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2237    1184 
   Size:       1053    4906
 
AlwNI CAG_nnn'CTG

Cuts at:   2320    3702    5274    2320 
   Size:       1382    1572    3005

  Fragments arranged by size: 

                3005    1572    1382
 
ApaI G_GGCC'C

Cuts at:   2037    2037 
   Size:       5959
 
ApaLI G'TGCA_C

Cuts at:   4016    5172    4016 
   Size:       1156    4803
 
ApoI r'AATT_y

Cuts at:   1493    3119    3239    3268    3494    3646    5755    1493 
   Size:       1626     120      29     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1626     226     152     120      29
 
AseI AT'TA_AT

Cuts at:    160    1032    4513     160 
   Size:        872    3481    1606

  Fragments arranged by size: 

                3481    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5959
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2038    2108    2853    3284    1870 
   Size:         73      95      70     745     431    4545

  Fragments arranged by size: 

                4545     745     431      95      73      70
 
BamHI G'GATC_C

Cuts at:   2953    3289    3656    2953 
   Size:        336     367    5256

  Fragments arranged by size: 

                5256     367     336
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2683    2702    2917    3280    4735 
   Size:        325     217     627     896      19     215     363    1455

Cuts at:   4735     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1455     896     627     363     325     217     215
                  19
 
BanII G_rGCy'C

Cuts at:   1391    2037    3278    1391 
   Size:        646    1241    4072

  Fragments arranged by size: 

                4072    1241     646
 
BbeI G_GCGC'C

Cuts at:   2687    2921    2687 
   Size:        234    5725
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2560    2759    3160     928 
   Size:        971     661     199     401    3727

  Fragments arranged by size: 

                3727     971     661     401     199
 
BbvCI CC'TCA_GC

Cuts at:   2282    2282 
   Size:       5959
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2119    2153    4150    4184    1903 
   Size:         34      50      34      98      34    1997      34    3678

  Fragments arranged by size: 

                3678    1997      98      50      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3851    5061    3851 
   Size:       1210    4749
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5959
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2177    2297    4570 
   Size:        107     122      71     631     605     505     120    2273

Cuts at:   4570     136 
   Size:       1525

  Fragments arranged by size: 

                2273    1525     631     605     505     122     120     107
                  71
 
BglII A'GATC_T

Cuts at:   5954    5954 
   Size:       5959
 
BlpI GC'TnA_GC

Cuts at:   2534    2546    3376    2534 
   Size:         12     830    5117

  Fragments arranged by size: 

                5117     830      12
 
Bme1580I G_kGCm'C

Cuts at:   1790    2037    4020    5176    1790 
   Size:        247    1983    1156    2573

  Fragments arranged by size: 

                2573    1983    1156     247
 
BmgBI CAC'GTC

Cuts at:   1318    2137    1318 
   Size:        819    5140
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3020    3690    4648 
   Size:        416      53      83     399      24    1603     670     958

Cuts at:   4648     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1603     958     670     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5959
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2032    2183    4619    1239 
   Size:        793     151    2436    2579

  Fragments arranged by size: 

                2579    2436     793     151
 
Bpu10I CC'TnA_GC

Cuts at:   2282    2282 
   Size:       5959
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2459    4084    4949    5247    2459 
   Size:       1625     865     298    3171

  Fragments arranged by size: 

                3171    1625     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4622     882 
   Size:        617    3123    2219

  Fragments arranged by size: 

                3123    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2653    3750     493 
   Size:        711    1449    1097    2702

  Fragments arranged by size: 

                2702    1449    1097     711
 
BsaBI GATnn'nnATC

Cuts at:   5953    5953 
   Size:       5959
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2240    2252    2684    2759 
   Size:         53      83     186     936     707      12     432      75

Cuts at:   2759    2918    3766    4148    4783     275 
   Size:        159     848     382     635    1451

  Fragments arranged by size: 

                1451     936     848     707     635     432     382     186
                 159      83      75      53      12
 
BsaWI w'CCGG_w

Cuts at:   2235    4392    4790    5064    5211    5622    2235 
   Size:       2157     398     274     147     411    2572

  Fragments arranged by size: 

                2572    2157     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5734

  Fragments arranged by size: 

                5734     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2162    1186 
   Size:        976    4983
 
BseYI C'CCAG_C

Cuts at:   2203    2334    2613    2913    5162    5658    2203 
   Size:        131     279     300    2249     496    2504

  Fragments arranged by size: 

                2504    2249     496     300     279     131
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2216    2799    1163 
   Size:        573     480     583    4323

  Fragments arranged by size: 

                4323     583     573     480
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2449    2523    3409    4170    5198    1540 
   Size:        275     186     448      74     886     761    1028    2301

  Fragments arranged by size: 

                2301    1028     886     761     448     275     186      74
            
 
BsiHKAI G_wGCw'C

Cuts at:   2552    4020    4105    5176    2552 
   Size:       1468      85    1071    3335

  Fragments arranged by size: 

                3335    1468    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2306    3470    3563    2306 
   Size:       1164      93    4702

  Fragments arranged by size: 

                4702    1164      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2015    2015 
   Size:       5959
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2037    2552    3278    4020    4105    5176    1391 
   Size:        399     247     515     726     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     726     515     399     247      85
            
 
BspEI T'CCGG_A

Cuts at:   5622    5622 
   Size:       5959
 
BspHI T'CATG_A

Cuts at:   2436    2511    3848    5578    2436 
   Size:         75    1337    1730    2817

  Fragments arranged by size: 

                2817    1730    1337      75
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2386    2400    2714    3314     844 
   Size:       1542      14     314     600    3489

  Fragments arranged by size: 

                3489    1542     600     314      14
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2473    2502    3846    1394 
   Size:        429     650      29    1344    3507

  Fragments arranged by size: 

                3507    1344     650     429      29
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4454    4628      65 
   Size:       1183    3206     174    1396

  Fragments arranged by size: 

                3206    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2018    2072    4603    4790    1700 
   Size:        110     208      54    2531     187    2869

  Fragments arranged by size: 

                2869    2531     208     187     110      54
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5959
 
BssHII G'CGCG_C

Cuts at:   1925    2495    1925 
   Size:        570    5389
 
BssSI C'ACGA_G

Cuts at:   2643    4019    5031    2643 
   Size:       1376    1012    3571

  Fragments arranged by size: 

                3571    1376    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5959
 
BstBI TT'CG_AA

Cuts at:   4763    4763 
   Size:       5959
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5959
 
BstYI r'GATC_y

Cuts at:   1972    2862    2953    3289    3656    4041    4058    5510    5596 
   Size:        890      91     336     367     385      17    1452      86

Cuts at:   5596    5608    5954    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     890     385     367     346     336      91
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2054    2301    2356    2688    2746     513 
   Size:        552     989     247      55     332      58    3726

  Fragments arranged by size: 

                3726     989     552     332     247      58      55
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2440     487 
   Size:        683      56    1214    4006

  Fragments arranged by size: 

                4006    1214     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3000    3475    4269    4289    3000 
   Size:        475     794      20    4670

  Fragments arranged by size: 

                4670     794     475      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5959
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5924
 
DraI TTT'AAA

Cuts at:   3263    3263 
   Size:       5959
 
DraIII CAC_nnn'GTG

Cuts at:   2828    2828 
   Size:       5959
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4966     809 
   Size:        507    3650    1802

  Fragments arranged by size: 

                3650    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2067    2154    2196 
   Size:         54    1425      50     275      18     237      87      42

Cuts at:   2196    2289    2298    2520    3061    3406    4295    5924       8 
   Size:         93       9     222     541     345     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     541     345     275     237     222
                  93      87      54      50      43      42      18       9
            
 
EagI C'GGCC_G

Cuts at:   1537    1812    2520    3406    1537 
   Size:        275     708     886    4090

  Fragments arranged by size: 

                4090     886     708     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2273    3889    1353 
   Size:        920    1616    3423

  Fragments arranged by size: 

                3423    1616     920
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2005    4546    4920    5066     387 
   Size:       1064     138     416    2541     374     146    1280

  Fragments arranged by size: 

                2541    1280    1064     416     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2032    2183    4022    4619    5406    5674    1239 
   Size:        393     400     151    1839     597     787     268    1524

  Fragments arranged by size: 

                1839    1524     787     597     400     393     268     151
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2111    2111 
   Size:       5959
 
EcoO109I rG'GnC_Cy

Cuts at:   2033    2034    2033 
   Size:          1    5958
 
EcoRI G'AATT_C

Cuts at:   3268    3646    3268 
   Size:        378    5581
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3177    3177 
   Size:       5959
 
FspI TGC'GCA

Cuts at:   2141    4465    2141 
   Size:       2324    3635
 
HaeII r_GCGC'y

Cuts at:   1678    2079    2687    2921    5106    1678 
   Size:        401     608     234    2185    2531

  Fragments arranged by size: 

                2531    2185     608     401     234
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2945    2977    4600    4632    4674 
   Size:         32     135      32    1373      32    1623      32      42

Cuts at:   4674    4706    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1623    1373     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3303    3549     669 
   Size:        988     281    1365     246    3079

  Fragments arranged by size: 

                3079    1365     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3088     748 
   Size:       2340    3619
 
HpaI GTT'AAC

Cuts at:   3549    3549 
   Size:       5959
 
KasI G'GCGC_C

Cuts at:   2683    2917    2683 
   Size:        234    5725
 
KpnI G_GTAC'C

Cuts at:   2706    3284    2706 
   Size:        578    5381
 
MfeI C'AATT_G

Cuts at:   3558    3558 
   Size:       5959
 
MluI A'CGCG_T

Cuts at:   5835    5835 
   Size:       5959
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2299    5073    5257    1305 
   Size:        994    2774     184    2007

  Fragments arranged by size: 

                2774    2007     994     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2069    2156    3063      10 
   Size:         54    1425     343     237      87     907    2906

  Fragments arranged by size: 

                2906    1425     907     343     237      87      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2459    2678    2936    3917    4276    4435     518 
   Size:        648    1293     219     258     981     359     159    2042

  Fragments arranged by size: 

                2042    1293     981     648     359     258     219     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    2358    2748    3375    4052    5200    5445    1606 
   Size:        752     390     627     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     752     677     627     390     245
 
NarI GG'CG_CC

Cuts at:   2684    2918    2684 
   Size:        234    5725
 
NcoI C'CATG_G

Cuts at:    513    1065    2054     513 
   Size:        552     989    4418

  Fragments arranged by size: 

                4418     989     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5959
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5959
 
NotI GC'GGCC_GC

Cuts at:   2520    3406    2520 
   Size:        886    5073
 
NspI r_CATG'y

Cuts at:   1132    1229    2263    2410    2428    3052    3317    4862    1132 
   Size:         97    1034     147      18     624     265    1545    2229

  Fragments arranged by size: 

                2229    1545    1034     624     265     147      97      18
            
 
PasI CC'CwG_GG

Cuts at:   1348    2634    1348 
   Size:       1286    4673
 
PciI A'CATG_T

Cuts at:   3048    4858    3048 
   Size:       1810    4149
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2055    1108 
   Size:        947    5012
 
PmeI GTTT'AAAC

Cuts at:   3263    3263 
   Size:       5959
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4028    4060    5541    5573    4028 
   Size:         32    1481      32    4414

  Fragments arranged by size: 

                4414    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5959
 
PsiI TTA'TAA

Cuts at:   3529    3529 
   Size:       5959
 
PspOMI G'GGCC_C

Cuts at:   2033    2033 
   Size:       5959
 
PstI C_TGCA'G

Cuts at:    830    2397    3311     830 
   Size:       1567     914    3478

  Fragments arranged by size: 

                3478    1567     914
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5959
 
SacII CC_GC'GG

Cuts at:   2359    2749    2359 
   Size:        390    5569
 
SalI G'TCGA_C

Cuts at:   1936    3301    1936 
   Size:       1365    4594
 
SbfI CC_TGCA'GG

Cuts at:   2397    3311    2397 
   Size:        914    5045
 
ScaI AGT'ACT

Cuts at:   2561    4207    5919    2561 
   Size:       1646    1712    2601

  Fragments arranged by size: 

                2601    1712    1646
 
SfcI C'TryA_G

Cuts at:    826    1045    2393    3307    4442    5123    5314     826 
   Size:        219    1348     914    1135     681     191    1471

  Fragments arranged by size: 

                1471    1348    1135     914     681     219     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2297    2297 
   Size:       5959
 
SfoI GGC'GCC

Cuts at:   2685    2919    2685 
   Size:        234    5725
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5959
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    2072    1700 
   Size:        372    5587
 
SmaI CCC'GGG

Cuts at:   1872    2855    3286    1872 
   Size:        983     431    4545

  Fragments arranged by size: 

                4545     983     431
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2474    4063    4964    5226     820 
   Size:        197     926     531    1589     901     262    1553

  Fragments arranged by size: 

                1589    1553     926     901     531     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3750     493 
   Size:       3257    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5959
 
SphI G_CATG'C

Cuts at:   1132    2263    3317    1132 
   Size:       1131    1054    3774

  Fragments arranged by size: 

                3774    1131    1054
 
SspI AAT'ATT

Cuts at:      5      52    3883       5 
   Size:         47    3831    2081

  Fragments arranged by size: 

                3831    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5959
 
StyI C'CwwG_G

Cuts at:    513    1065    2054    2598    3398     513 
   Size:        552     989     544     800    3074

  Fragments arranged by size: 

                3074     989     800     552     544
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2518    2987    3986    4003    4156    4341    4786 
   Size:       1255     938     469     999      17     153     185     445

Cuts at:   4786     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255     999     938     469     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2559    4205    5917 
   Size:        274      80      33      51    1443     582    1646    1712

Cuts at:   5917      96 
   Size:        138

  Fragments arranged by size: 

                1712    1646    1443     582     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5919    5951    5919 
   Size:         32    5927
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2758    1316 
   Size:       1442    4517
 
XbaI T'CTAG_A

Cuts at:   3295    3295 
   Size:       5959
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5959
 
XmaI C'CCGG_G

Cuts at:   1870    2853    3284    1870 
   Size:        983     431    4545

  Fragments arranged by size: 

                4545     983     431
 
XmnI GAAnn'nnTTC

Cuts at:   4088    4088 
   Size:       5959
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2253    3767     276 
   Size:         53      83     186     936     719    1514    2468

  Fragments arranged by size: 

                2468    1514     936     719     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI     PstI     PvuI
    SacII     SalI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BaeI    BfrBI     BplI    BsiWI
    BstXI  BstZ17I   Bsu36I  EcoICRI    EcoRV     FseI    FspAI     NaeI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI    PspXI
     PsrI    PvuII    RsrII     SacI    SanDI     SapI    SexAI     SrfI
     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com