Restriction map for
pFN21AB7552
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbrPI BbsI BcgI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FokI FseI FspI
HgaI HincII HindII HpaI HphI
KpnI KspI MaeII MamI MfeI
MluI MseI MunI NaeI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 5 CGCG CG^CG
AccIII 229 TCCGGA T^CCGGA
AcyI 514 GGCGCC G[GA]^CG[CT]C
AlwI 138 GATCC GGATC(4/5)
AlwNI 585 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 428 TGGCCA TGG^CCA
BanI 514 GGCGCC G^G[CT][GA]CC
BanII 686 GGGCTC G[GA]GC[CT]^C
BbeI 514 GGCGCC GGCGC^C
BbvCI 141 CCTCAGC CCTCAGC(-5/-2)
BciVI 232 GGATAC GTATCC(6/5)
BfaI 464 CTAG C^TAG
BglII 563 AGATCT A^GATCT
BmyI 686 GGGCTC G[AGT]GC[ACT]^C
BpmI 750 CTCCAG CTGGAG(16/14)
BsaHI 514 GGCGCC G[GA]^CG[CT]C
BsaWI 229 TCCGGA [AT]^CCGG[AT]
BseAI 229 TCCGGA T^CCGGA
BsmAI 759 GTCTC GTCTC(1/5)
BsmFI 260 GTCCC GGGAC(10/14)
Bsp1286I 686 GGGCTC G[AGT]GC[ACT]^C
BspEI 229 TCCGGA T^CCGGA
BstUI 5 CGCG CG^CG
Cac8I 426 GCTGGC GC[ACGT]^[ACGT]GC
DraIII 168 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 428 TGGCCA [CT]^GGCC[GA]
EarI 325 GAAGAG CTCTTC(1/4)
EcoT14I 287 CCAAGG C^C[AT][AT]GG
EcoT22I 526 ATGCAT ATGCA^T
HaeII 514 GGCGCC [GA]GCGC^[CT]
HapII 230 CCGG C^CGG
Hin1I 514 GGCGCC G[GA]^CG[CT]C
HindIII 346 AAGCTT A^AGCTT
HpaII 230 CCGG C^CGG
KasI 514 GGCGCC G^GCGCC
Ksp632I 325 GAAGAG CTCTTC(1/4)
MaeI 464 CTAG C^TAG
MluNI 428 TGGCCA TGG^CCA
MroI 229 TCCGGA T^CCGGA
MscI 428 TGGCCA TGG^CCA
MspI 230 CCGG C^CGG
MvnI 5 CGCG CG^CG
NarI 514 GGCGCC GG^CGCC
NsiI 526 ATGCAT ATGCA^T
PflMI 584 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 258 GAGTC GAGTC(4/5)
PshAI 335 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 694 CTGCAG CTGCA^G
SfoI 514 GGCGCC GGC^GCC
StyI 287 CCAAGG C^C[AT][AT]GG
TfiI 781 GAATC G^A[AT]TC
Tsp45I 491 GTGAC ^GT[GC]AC
Tsp509I 391 AATT ^AATT
Van91I 584 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 463 TCTAGA T^CTAGA
XcmI 201 CCAGCTACCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Asp700I 24 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
376 GAAAAGTTTC
AvaII 291 GGACC G^G[AT]CC
536 GGACC
BsgI 445 CTGCAC GTGCAG(16/14)
766 CTGCAC
BsiYI 223 CCTCTTTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
584 CCAGACCCTGG
BslI 223 CCTCTTTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
584 CCAGACCCTGG
BstYI 137 AGATCC [GA]^GATC[CT]
563 AGATCT
CfoI 6 GCGC GCG^C
515 GCGC
Cfr13I 291 GGACC G^G[ACGT]CC
536 GGACC
DpnII 138 GATC ^GATC
564 GATC
HaeIII 214 GGCC GG^CC
429 GGCC
HhaI 6 GCGC GCG^C
515 GCGC
HinP1I 6 GCGC G^CGC
515 GCGC
HinfI 258 GAGTC G^A[ACGT]TC
781 GAATC
MaeIII 107 GTTAC ^GT[ACGT]AC
491 GTGAC
MboI 138 GATC ^GATC
564 GATC
MboII 325 GAAGA GAAGA(8/7)
522 GAAGA
MflI 137 AGATCC [GA]^GATC[CT]
563 AGATCT
NdeII 138 GATC ^GATC
564 GATC
NlaIV 514 GGCGCC GG[ACGT]^[ACGT]CC
536 GGACCC
PvuII 442 CAGCTG CAG^CTG
477 CAGCTG
Sau3AI 138 GATC ^GATC
564 GATC
Sau96I 291 GGACC G^G[ACGT]CC
536 GGACC
SfaNI 454 GATGC GCATC(5/9)
525 GATGC
XhoII 137 AGATCC [GA]^GATC[CT]
563 AGATCT
XmnI 24 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
376 GAAAAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BbvI BsaJI BseRI
BsrI BssKI BstAPI BstNI DdeI
EcoRII Fnu4HI ItaI MnlI MslI
MspA1I MvaI MwoI NlaIII ScrFI
SfcI TseI TspRI