Restriction map for
pFN21AB7552
(Linear) (Six-base) MAPSORT of: KIBB7552.seq Check: 2132 from: 1 to: 804
KIBB7552.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 804
Size: 541 263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 470 804
Size: 470 334
AlwNI CAG_nnn'CTG
Cuts at: 0 590 804
Size: 590 214
BanI G'GyrC_C
Cuts at: 0 514 804
Size: 514 290
BanII G_rGCy'C
Cuts at: 0 690 804
Size: 690 114
BbeI G_GCGC'C
Cuts at: 0 518 804
Size: 518 286
BbvCI CC'TCA_GC
Cuts at: 0 142 804
Size: 142 662
BciVI GTATCCnnnnn_n'
Cuts at: 0 226 804
Size: 226 578
BfrBI ATG'CAT
Cuts at: 0 528 804
Size: 528 276
BglII A'GATC_T
Cuts at: 0 563 804
Size: 563 241
BmrI ACTGGGnnnn_n'
Cuts at: 0 453 804
Size: 453 351
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 281 804
Size: 126 155 523
Fragments arranged by size:
523 155 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 804
Size: 735 69
Bpu10I CC'TnA_GC
Cuts at: 0 142 147 804
Size: 142 5 657
Fragments arranged by size:
657 142 5
BsaHI Gr'CG_yC
Cuts at: 0 515 804
Size: 515 289
BsaWI w'CCGG_w
Cuts at: 0 229 804
Size: 229 575
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 171 201 268 298 804
Size: 171 30 67 30 506
Fragments arranged by size:
506 171 67 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 212 228 257 273 804
Size: 212 16 29 16 531
Fragments arranged by size:
531 212 29 16 16
BseYI C'CCAG_C
Cuts at: 0 200 804
Size: 200 604
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 751 804
Size: 430 321 53
Fragments arranged by size:
430 321 53
Bsp1286I G_dGCh'C
Cuts at: 0 690 804
Size: 690 114
BspEI T'CCGG_A
Cuts at: 0 229 804
Size: 229 575
BstAPI GCAn_nnn'nTGC
Cuts at: 0 395 566 734 804
Size: 395 171 168 70
Fragments arranged by size:
395 171 168 70
BstYI r'GATC_y
Cuts at: 0 137 563 804
Size: 137 426 241
Fragments arranged by size:
426 241 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 467 804
Size: 467 337
BtsI GCAGTG_nn'
Cuts at: 0 447 648 804
Size: 447 201 156
Fragments arranged by size:
447 201 156
DraIII CAC_nnn'GTG
Cuts at: 0 173 804
Size: 173 631
EaeI y'GGCC_r
Cuts at: 0 428 804
Size: 428 376
EarI CTCTTCn'nnn_
Cuts at: 0 320 804
Size: 320 484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 735 804
Size: 541 194 69
Fragments arranged by size:
541 194 69
HaeII r_GCGC'y
Cuts at: 0 518 804
Size: 518 286
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 445 477 675 707 804
Size: 268 32 145 32 198 32 97
Fragments arranged by size:
268 198 145 97 32 32 32
HindIII A'AGCT_T
Cuts at: 0 346 804
Size: 346 458
KasI G'GCGC_C
Cuts at: 0 514 804
Size: 514 290
MscI TGG'CCA
Cuts at: 0 430 804
Size: 430 374
MslI CAynn'nnrTG
Cuts at: 0 169 452 707 773 804
Size: 169 283 255 66 31
Fragments arranged by size:
283 255 169 66 31
MspA1I CmG'CkG
Cuts at: 0 184 444 479 804
Size: 184 260 35 325
Fragments arranged by size:
325 260 184 35
NarI GG'CG_CC
Cuts at: 0 515 804
Size: 515 289
NsiI A_TGCA'T
Cuts at: 0 530 804
Size: 530 274
PflMI CCAn_nnn'nTGG
Cuts at: 0 590 804
Size: 590 214
PshAI GACnn'nnGTC
Cuts at: 0 339 804
Size: 339 465
PstI C_TGCA'G
Cuts at: 0 698 804
Size: 698 106
PvuII CAG'CTG
Cuts at: 0 444 479 804
Size: 444 35 325
Fragments arranged by size:
444 325 35
SfcI C'TryA_G
Cuts at: 0 54 337 694 804
Size: 54 283 357 110
Fragments arranged by size:
357 283 110 54
SfoI GGC'GCC
Cuts at: 0 516 804
Size: 516 288
StyI C'CwwG_G
Cuts at: 0 287 804
Size: 287 517
XbaI T'CTAG_A
Cuts at: 0 463 804
Size: 463 341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 208 804
Size: 208 596
XmnI GAAnn'nnTTC
Cuts at: 0 28 380 804
Size: 28 352 424
Fragments arranged by size:
424 352 28
Enzymes that do cut:
AcuI AlfI AlwNI BanI BanII BbeI BbvCI BciVI
BfrBI BglII BmrI BplI BpmI Bpu10I BsaHI BsaWI
BsaXI BseRI BseYI BsgI Bsp1286I BspEI BstAPI BstYI
BtgZI BtsI DraIII EaeI EarI Eco57MI HaeII Hin4I
HindIII KasI MscI MslI MspA1I NarI NsiI PflMI
PshAI PstI PvuII SfcI SfoI StyI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbsI BcgI
BclI BglI BlpI Bme1580I BmgBI BmtI BpuEI BsaI
BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KpnI MfeI
MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XhoI XmaI ZraI