(Circular) (Six-base) MAPSORT of: pFN21AB9431.seq Check: 918 from: 1 to: 6277 pFN21AB9431 6277 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2126 2357 2126 Size: 231 6046 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3264 3620 1937 Size: 1327 356 4594 Fragments arranged by size: 4594 1327 356 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2444 3000 3164 3564 4340 5724 5992 1632 Size: 812 556 164 400 776 1384 268 1917 Fragments arranged by size: 1917 1384 812 776 556 400 268 164 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 5176 6153 1203 Size: 3973 977 1327 Fragments arranged by size: 3973 1327 977 AgeI A'CCGG_T Cuts at: 2844 5108 2844 Size: 2264 4013 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 3431 1166 Size: 2265 4012 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2628 1184 Size: 1444 4833 AlwNI CAG_nnn'CTG Cuts at: 2593 2641 3489 4020 5592 2593 Size: 48 848 531 1572 3278 Fragments arranged by size: 3278 1572 848 531 48 ApaI G_GGCC'C Cuts at: 3416 3416 Size: 6277 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 3586 3812 3964 6073 1493 Size: 2093 226 152 2109 1697 Fragments arranged by size: 2109 2093 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4831 160 Size: 872 3799 1606 Fragments arranged by size: 3799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 2064 3308 3602 1870 Size: 73 121 1244 294 4545 Fragments arranged by size: 4545 1244 294 121 73 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3332 3598 5053 618 Size: 325 217 627 1545 266 1455 1842 Fragments arranged by size: 1842 1545 1455 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 2904 3246 3317 3344 3416 3483 3576 3596 Size: 1513 342 71 27 72 67 93 20 Cuts at: 3596 1391 Size: 4072 Fragments arranged by size: 4072 1513 342 93 72 71 67 27 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2520 3057 3564 928 Size: 971 621 537 507 3641 Fragments arranged by size: 3641 971 621 537 507 BbvCI CC'TCA_GC Cuts at: 3045 3045 Size: 6277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3103 3137 4468 4502 1903 Size: 34 50 34 1082 34 1331 34 3678 Fragments arranged by size: 3678 1331 1082 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4169 5379 4169 Size: 1210 5067 BclI T'GATC_A Cuts at: 1548 2149 1548 Size: 601 5676 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3107 4888 136 Size: 107 122 71 631 605 1435 1781 1525 Fragments arranged by size: 1781 1525 1435 631 605 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 2928 3416 4338 5494 1790 Size: 1138 488 922 1156 2573 Fragments arranged by size: 2573 1156 1138 922 488 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4008 4966 Size: 416 53 83 399 24 2060 531 958 Cuts at: 4966 442 Size: 1753 Fragments arranged by size: 2060 1753 958 531 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3194 4937 1239 Size: 1955 1743 2579 Fragments arranged by size: 2579 1955 1743 Bpu10I CC'TnA_GC Cuts at: 2763 3045 2763 Size: 282 5995 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4402 5267 5565 4402 Size: 865 298 5114 Fragments arranged by size: 5114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2695 3449 4940 882 Size: 617 1196 754 1491 2219 Fragments arranged by size: 2219 1491 1196 754 617 BsaAI yAC'GTr Cuts at: 493 1204 4068 493 Size: 711 2864 2702 Fragments arranged by size: 2864 2702 711 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2331 2796 3515 Size: 53 83 186 936 537 261 465 719 Cuts at: 3515 4084 4466 5101 275 Size: 569 382 635 1451 Fragments arranged by size: 1451 936 719 635 569 537 465 382 261 186 83 53 BsaWI w'CCGG_w Cuts at: 2844 4710 5108 5382 5529 5940 2844 Size: 1866 398 274 147 411 3181 Fragments arranged by size: 3181 1866 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 1178 Size: 30 165 30 960 30 5062 Fragments arranged by size: 5062 960 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2477 2762 2924 2957 2960 2963 2966 2969 Size: 1291 285 162 33 3 3 3 3 Cuts at: 2969 2972 2975 2978 3002 3005 3008 3011 3140 Size: 3 3 3 24 3 3 3 129 Cuts at: 3140 3170 1186 Size: 30 4293 Fragments arranged by size: 4293 1291 285 162 129 33 30 24 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2040 2495 5480 5976 2040 Size: 455 2985 496 2341 Fragments arranged by size: 2985 2341 496 455 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2218 3032 1163 Size: 573 482 814 4408 Fragments arranged by size: 4408 814 573 482 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2815 3140 3727 4488 5516 1540 Size: 275 186 814 325 587 761 1028 2301 Fragments arranged by size: 2301 1028 814 761 587 325 275 186 BsiHKAI G_wGCw'C Cuts at: 3246 3576 4338 4423 5494 3246 Size: 330 762 85 1071 4029 Fragments arranged by size: 4029 1071 762 330 85 BsmI GAATG_Cn' Cuts at: 3788 3881 3788 Size: 93 6184 BsmBI CGTCTCn'nnnn_ Cuts at: 2077 2077 Size: 6277 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2904 2928 3246 3317 3344 3416 3483 Size: 399 1114 24 318 71 27 72 67 Cuts at: 3483 3576 3596 4338 4423 5494 1391 Size: 93 20 742 85 1071 2174 Fragments arranged by size: 2174 1114 1071 742 399 318 93 85 72 71 67 27 24 20 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 4166 5896 4166 Size: 1730 4547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2126 2357 3632 844 Size: 1282 231 1275 3489 Fragments arranged by size: 3489 1282 1275 231 BsrBI CCG'CTC Cuts at: 1394 1823 3136 3423 4164 1394 Size: 429 1313 287 741 3507 Fragments arranged by size: 3507 1313 741 429 287 BsrDI GCAATG_nn' Cuts at: 65 1248 4772 4946 65 Size: 1183 3524 174 1396 Fragments arranged by size: 3524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2844 3031 4921 5108 1700 Size: 110 1034 187 1890 187 2869 Fragments arranged by size: 2869 1890 1034 187 187 110 BsrGI T'GTAC_A Cuts at: 96 3225 96 Size: 3129 3148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 2882 3402 794 Size: 2088 520 3669 Fragments arranged by size: 3669 2088 520 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 2401 3463 1184 Size: 1217 1062 3998 Fragments arranged by size: 3998 1217 1062 BstYI r'GATC_y Cuts at: 1972 2430 3607 3974 4359 4376 5828 5914 5926 Size: 458 1177 367 385 17 1452 86 12 Cuts at: 5926 6272 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1177 458 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2046 3459 513 Size: 552 981 1413 3331 Fragments arranged by size: 3331 1413 981 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2319 487 Size: 683 56 1093 4445 Fragments arranged by size: 4445 1093 683 56 BtsI GCAGTG_nn' Cuts at: 2230 2855 3793 4587 4607 2230 Size: 625 938 794 20 3900 Fragments arranged by size: 3900 938 794 625 20 ClaI AT'CG_AT Cuts at: 1554 3093 1554 Size: 1539 4738 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3310 3345 529 Size: 35 2746 35 3461 Fragments arranged by size: 3461 2746 35 35 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2687 5284 809 Size: 507 1371 2597 1802 Fragments arranged by size: 2597 1802 1371 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2394 3033 Size: 54 1425 50 275 18 303 261 639 Cuts at: 3033 3724 4613 6242 8 Size: 691 889 1629 43 Fragments arranged by size: 1629 1425 889 691 639 303 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2595 2967 2979 4207 1353 Size: 1242 372 12 1228 3423 Fragments arranged by size: 3423 1242 1228 372 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3244 3574 3244 Size: 330 5947 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2444 3000 3164 3194 3564 4340 4937 Size: 393 812 556 164 30 370 776 597 Cuts at: 4937 5724 5992 1239 Size: 787 268 1524 Fragments arranged by size: 1524 812 787 776 597 556 393 370 268 164 30 EcoNI CCTnn'n_nnAGG Cuts at: 2438 2438 Size: 6277 EcoO109I rG'GnC_Cy Cuts at: 2061 2767 3412 3413 2061 Size: 706 645 1 4925 Fragments arranged by size: 4925 706 645 1 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 5424 1678 Size: 3746 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 2786 2818 4918 Size: 32 135 32 791 32 391 32 2100 Cuts at: 4918 4950 4992 5024 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2100 791 391 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2158 2281 3621 3867 669 Size: 988 281 220 123 1340 246 3079 Fragments arranged by size: 3079 1340 988 281 246 220 123 HindIII A'AGCT_T Cuts at: 748 3284 748 Size: 2536 3741 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2295 5391 5575 1305 Size: 990 3096 184 2007 Fragments arranged by size: 3096 2007 990 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4455 Fragments arranged by size: 4455 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3431 4235 4594 4753 518 Size: 648 2265 804 359 159 2042 Fragments arranged by size: 2265 2042 804 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2328 2608 3693 4370 5518 5763 1606 Size: 411 311 280 1085 677 1148 245 2120 Fragments arranged by size: 2120 1148 1085 677 411 311 280 245 NaeI GCC'GGC Cuts at: 3033 3033 Size: 6277 NcoI C'CATG_G Cuts at: 513 1065 3459 513 Size: 552 2394 3331 Fragments arranged by size: 3331 2394 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NgoMIV G'CCGG_C Cuts at: 3031 3031 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 3635 5180 1132 Size: 97 2406 1545 2229 Fragments arranged by size: 2406 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6277 PciI A'CATG_T Cuts at: 5176 5176 Size: 6277 PflMI CCAn_nnn'nTGG Cuts at: 1108 2040 1108 Size: 932 5345 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PspOMI G'GGCC_C Cuts at: 3412 3412 Size: 6277 PstI C_TGCA'G Cuts at: 830 2388 2709 3629 830 Size: 1558 321 920 3478 Fragments arranged by size: 3478 1558 920 321 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2608 2608 Size: 6277 RsrII CG'GwC_CG Cuts at: 2847 2847 Size: 6277 SacI G_AGCT'C Cuts at: 3246 3576 3246 Size: 330 5947 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SapI GCTCTTCn'nnn_ Cuts at: 2595 2595 Size: 6277 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SexAI A'CCwGG_T Cuts at: 2517 2517 Size: 6277 SfcI C'TryA_G Cuts at: 826 1045 2384 2489 2705 3625 4760 5441 5632 Size: 219 1339 105 216 920 1135 681 191 Cuts at: 5632 826 Size: 1471 Fragments arranged by size: 1471 1339 1135 920 681 219 216 191 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 2066 3604 1872 Size: 194 1538 4545 Fragments arranged by size: 4545 1538 194 SmlI C'TyrA_G Cuts at: 820 1017 1943 4381 5282 5544 820 Size: 197 926 2438 901 262 1553 Fragments arranged by size: 2438 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 3086 1517 Size: 1569 4708 StyI C'CwwG_G Cuts at: 513 1065 2619 2904 3328 3459 3716 513 Size: 552 1554 285 424 131 257 3074 Fragments arranged by size: 3074 1554 552 424 285 257 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3565 4304 4321 4474 4659 5104 325 Size: 1255 1985 739 17 153 185 445 1498 Fragments arranged by size: 1985 1498 1255 739 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2319 3225 4523 Size: 274 80 33 51 1443 342 906 1298 Cuts at: 4523 6235 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1298 906 342 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2854 2886 6237 6269 2854 Size: 32 3351 32 2862 Fragments arranged by size: 3351 2862 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2816 1316 Size: 1500 4777 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3169 3169 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 2064 3602 1870 Size: 194 1538 4545 Fragments arranged by size: 4545 1538 194 XmnI GAAnn'nnTTC Cuts at: 4406 4406 Size: 6277 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacII SanDI SfiI SfoI SrfI SwaI