(Circular) (Six-base) MAPSORT of: pFN21ASDA0679.seq Check: 8376 from: 1 to: 7477 pFN21ASDA0679 7477 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5287 278 Size: 53 83 186 936 3751 2468 Fragments arranged by size: 3751 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4436 4820 1937 Size: 2499 384 4594 Fragments arranged by size: 4594 2499 384 Acc65I G'GTAC_C Cuts at: 4798 4798 Size: 7477 AclI AA'CG_TT Cuts at: 1563 5604 5977 1563 Size: 4041 373 3063 Fragments arranged by size: 4041 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4008 4646 5540 6924 7192 1632 Size: 2376 638 894 1384 268 1917 Fragments arranged by size: 2376 1917 1384 894 638 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7280 AflIII A'CryG_T Cuts at: 1203 3939 6376 7353 1203 Size: 2736 2437 977 1327 Fragments arranged by size: 2736 2437 1327 977 AgeI A'CCGG_T Cuts at: 6308 6308 Size: 7477 AhdI GACnn_n'nnGTC Cuts at: 6206 6206 Size: 7477 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7477 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3720 1184 Size: 2536 4941 AlwNI CAG_nnn'CTG Cuts at: 5220 6792 5220 Size: 1572 5905 ApaI G_GGCC'C Cuts at: 2906 2906 Size: 7477 ApaLI G'TGCA_C Cuts at: 5534 6690 5534 Size: 1156 6321 ApoI r'AATT_y Cuts at: 1493 2796 2972 3764 4404 4680 4688 4786 5012 Size: 1303 176 792 640 276 8 98 226 Cuts at: 5012 5164 7273 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1303 792 640 276 226 176 152 98 8 AseI AT'TA_AT Cuts at: 160 1032 6031 160 Size: 872 4999 1606 Fragments arranged by size: 4999 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7477 AvaI C'yCGr_G Cuts at: 1870 1943 4641 4802 1870 Size: 73 2698 161 4545 Fragments arranged by size: 4545 2698 161 73 AvrII C'CTAG_G Cuts at: 2638 2638 Size: 7477 BamHI G'GATC_C Cuts at: 4413 4807 5174 4413 Size: 394 367 6716 Fragments arranged by size: 6716 394 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 4798 6253 618 Size: 325 217 627 341 2670 1455 1842 Fragments arranged by size: 2670 1842 1455 627 341 325 217 BanII G_rGCy'C Cuts at: 1391 2073 2194 2845 2906 3330 4796 1391 Size: 682 121 651 61 424 1466 4072 Fragments arranged by size: 4072 1466 682 651 424 121 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2145 2239 4611 928 Size: 971 246 94 2372 3794 Fragments arranged by size: 3794 2372 971 246 94 BbvCI CC'TCA_GC Cuts at: 4307 4307 Size: 7477 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5668 5702 1903 Size: 34 3731 34 3678 Fragments arranged by size: 3731 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2485 5369 6579 2485 Size: 2884 1210 3383 Fragments arranged by size: 3383 2884 1210 BclI T'GATC_A Cuts at: 1548 2881 1548 Size: 1333 6144 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6088 136 Size: 107 122 71 631 605 4416 1525 Fragments arranged by size: 4416 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7472 7472 Size: 7477 BlpI GC'TnA_GC Cuts at: 2072 2370 4894 2072 Size: 298 2524 4655 Fragments arranged by size: 4655 2524 298 Bme1580I G_kGCm'C Cuts at: 1790 2906 4370 5538 6694 1790 Size: 1116 1464 1168 1156 2573 Fragments arranged by size: 2573 1464 1168 1156 1116 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7477 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5208 6166 442 Size: 416 53 83 399 24 3791 958 1753 Fragments arranged by size: 3791 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3170 1056 Size: 2114 5363 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2092 2092 Size: 7477 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2261 2829 3707 6137 1239 Size: 890 132 568 878 2430 2579 Fragments arranged by size: 2579 2430 890 878 568 132 Bpu10I CC'TnA_GC Cuts at: 4307 4307 Size: 7477 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3373 5602 6467 6765 3373 Size: 2229 865 298 4085 Fragments arranged by size: 4085 2229 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4038 4657 6140 882 Size: 617 2539 619 1483 2219 Fragments arranged by size: 2539 2219 1483 619 617 BsaAI yAC'GTr Cuts at: 493 1204 4774 5268 493 Size: 711 3570 494 2702 Fragments arranged by size: 3570 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2880 7471 2880 Size: 4591 2886 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5284 5666 6301 275 Size: 53 83 186 936 3751 382 635 1451 Fragments arranged by size: 3751 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5910 6308 6582 6729 7140 5910 Size: 398 274 147 411 6247 Fragments arranged by size: 6247 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2154 2184 3929 3959 1178 Size: 30 165 30 751 30 1745 30 4696 Fragments arranged by size: 4696 1745 751 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 1186 Size: 883 6594 BseYI C'CCAG_C Cuts at: 2156 6680 7176 2156 Size: 4524 496 2457 Fragments arranged by size: 4524 2457 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 2382 2539 2586 1163 Size: 573 312 334 157 47 6054 Fragments arranged by size: 6054 573 334 312 157 47 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4927 5688 6716 1540 Size: 275 186 2926 761 1028 2301 Fragments arranged by size: 2926 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 2845 3751 5538 5623 6694 2073 Size: 772 906 1787 85 1071 2856 Fragments arranged by size: 2856 1787 1071 906 772 85 BsmI GAATG_Cn' Cuts at: 4988 5081 4988 Size: 93 7384 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2194 2845 2906 3330 3751 4370 Size: 399 283 121 651 61 424 421 619 Cuts at: 4370 4796 5538 5623 6694 1391 Size: 426 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 619 426 424 421 399 283 121 85 61 BspEI T'CCGG_A Cuts at: 7140 7140 Size: 7477 BspHI T'CATG_A Cuts at: 5366 7096 5366 Size: 1730 5747 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4832 844 Size: 3988 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2059 2165 5364 1394 Size: 429 236 106 3199 3507 Fragments arranged by size: 3507 3199 429 236 106 BsrDI GCAATG_nn' Cuts at: 65 1248 3591 5972 6146 65 Size: 1183 2343 2381 174 1396 Fragments arranged by size: 2381 2343 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2116 6121 6308 1700 Size: 110 306 4005 187 2869 Fragments arranged by size: 4005 2869 306 187 110 BsrGI T'GTAC_A Cuts at: 96 3663 3994 4206 96 Size: 3567 331 212 3367 Fragments arranged by size: 3567 3367 331 212 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7477 BssSI C'ACGA_G Cuts at: 2400 3849 5537 6549 2400 Size: 1449 1688 1012 3328 Fragments arranged by size: 3328 1688 1449 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3637 794 Size: 2843 4634 BstBI TT'CG_AA Cuts at: 2007 6281 2007 Size: 4274 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7477 BstXI CCAn_nnnn'nTGG Cuts at: 2853 3421 2853 Size: 568 6909 BstYI r'GATC_y Cuts at: 1972 2918 4413 4807 5174 5559 5576 7028 7114 Size: 946 1495 394 367 385 17 1452 86 Cuts at: 7114 7126 7472 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1495 1452 946 394 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4437 4437 Size: 7477 Bsu36I CC'TnA_GG Cuts at: 4188 4188 Size: 7477 BtgI C'CryG_G Cuts at: 513 1065 2022 2936 3414 513 Size: 552 957 914 478 4576 Fragments arranged by size: 4576 957 914 552 478 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2088 487 Size: 683 56 862 5876 Fragments arranged by size: 5876 862 683 56 BtsI GCAGTG_nn' Cuts at: 3118 3666 4993 5787 5807 3118 Size: 548 1327 794 20 4788 Fragments arranged by size: 4788 1327 794 548 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7477 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4728 4763 529 Size: 35 4164 35 3243 Fragments arranged by size: 4164 3243 35 35 DraI TTT'AAA Cuts at: 3291 4781 3291 Size: 1490 5987 DraIII CAC_nnn'GTG Cuts at: 3236 3236 Size: 7477 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6484 809 Size: 507 5168 1802 Fragments arranged by size: 5168 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 4590 4924 Size: 54 1425 50 275 18 342 2418 334 Cuts at: 4924 5813 7442 8 Size: 889 1629 43 Fragments arranged by size: 2418 1629 1425 889 342 334 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4924 1537 Size: 275 3112 4090 Fragments arranged by size: 4090 3112 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2666 2811 2882 3861 5407 1353 Size: 1313 145 71 979 1546 3423 Fragments arranged by size: 3423 1546 1313 979 145 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6064 6438 6584 387 Size: 1064 138 4475 374 146 1280 Fragments arranged by size: 4475 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2071 2843 2071 Size: 772 6705 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2261 2829 3707 4008 4646 5540 Size: 393 497 132 568 878 301 638 894 Cuts at: 5540 6137 6924 7192 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 894 878 787 638 597 568 497 393 301 268 132 EcoO109I rG'GnC_Cy Cuts at: 2774 2774 Size: 7477 EcoRI G'AATT_C Cuts at: 4680 4786 5164 4680 Size: 106 378 6993 Fragments arranged by size: 6993 378 106 EcoRV GAT'ATC Cuts at: 2449 3961 2449 Size: 1512 5965 FspI TGC'GCA Cuts at: 5983 5983 Size: 7477 HaeII r_GCGC'y Cuts at: 1678 6624 1678 Size: 4946 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2433 2465 3929 3961 4184 Size: 32 135 32 861 32 1464 32 223 Cuts at: 4184 4216 6118 6150 6192 6224 1373 Size: 32 1902 32 42 32 2626 Fragments arranged by size: 2626 1902 1464 861 223 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4821 5067 669 Size: 988 281 2883 246 3079 Fragments arranged by size: 3079 2883 988 281 246 HindIII A'AGCT_T Cuts at: 748 2741 748 Size: 1993 5484 HpaI GTT'AAC Cuts at: 5067 5067 Size: 7477 KpnI G_GTAC'C Cuts at: 4802 4802 Size: 7477 MfeI C'AATT_G Cuts at: 3805 3910 4104 5076 3805 Size: 105 194 972 6206 Fragments arranged by size: 6206 972 194 105 MluI A'CGCG_T Cuts at: 7353 7353 Size: 7477 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2031 2103 4742 6591 6775 1305 Size: 726 72 2639 1849 184 2007 Fragments arranged by size: 2639 2007 1849 726 184 72 MscI TGG'CCA Cuts at: 10 64 1489 1832 4592 10 Size: 54 1425 343 2760 2895 Fragments arranged by size: 2895 2760 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2871 3419 3425 3456 5435 5794 5953 Size: 648 1705 548 6 31 1979 359 159 Cuts at: 5953 518 Size: 2042 Fragments arranged by size: 2042 1979 1705 648 548 359 159 31 6 MspA1I CmG'CkG Cuts at: 1606 4893 5570 6718 6963 1606 Size: 3287 677 1148 245 2120 Fragments arranged by size: 3287 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2118 2118 Size: 7477 NcoI C'CATG_G Cuts at: 513 1065 2022 3414 513 Size: 552 957 1392 4576 Fragments arranged by size: 4576 1392 957 552 NdeI CA'TA_TG Cuts at: 387 4349 387 Size: 3962 3515 NgoMIV G'CCGG_C Cuts at: 2116 2116 Size: 7477 NheI G'CTAG_C Cuts at: 1052 3166 1052 Size: 2114 5363 NotI GC'GGCC_GC Cuts at: 4924 4924 Size: 7477 NspI r_CATG'y Cuts at: 1132 1229 3943 4835 6380 1132 Size: 97 2714 892 1545 2229 Fragments arranged by size: 2714 2229 1545 892 97 PasI CC'CwG_GG Cuts at: 1348 4056 1348 Size: 2708 4769 PciI A'CATG_T Cuts at: 3939 6376 3939 Size: 2437 5040 PflMI CCAn_nnn'nTGG Cuts at: 1108 4109 1108 Size: 3001 4476 PfoI T'CCnGG_A Cuts at: 4409 4409 Size: 7477 PmeI GTTT'AAAC Cuts at: 4781 4781 Size: 7477 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2402 2434 5546 5578 7059 7091 2402 Size: 32 3112 32 1481 32 2788 Fragments arranged by size: 3112 2788 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2774 2774 Size: 7477 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7477 PsiI TTA'TAA Cuts at: 5047 5047 Size: 7477 PspOMI G'GGCC_C Cuts at: 2902 2902 Size: 7477 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4494 4526 4494 Size: 32 7445 PstI C_TGCA'G Cuts at: 830 2185 4829 830 Size: 1355 2644 3478 Fragments arranged by size: 3478 2644 1355 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7477 SacI G_AGCT'C Cuts at: 2073 2845 2073 Size: 772 6705 SalI G'TCGA_C Cuts at: 1936 4819 1936 Size: 2883 4594 SbfI CC_TGCA'GG Cuts at: 4829 4829 Size: 7477 ScaI AGT'ACT Cuts at: 3076 4512 5725 7437 3076 Size: 1436 1213 1712 3116 Fragments arranged by size: 3116 1712 1436 1213 SexAI A'CCwGG_T Cuts at: 2499 3011 2499 Size: 512 6965 SfcI C'TryA_G Cuts at: 826 1045 2181 3142 4371 4825 5960 6641 6832 Size: 219 1136 961 1229 454 1135 681 191 Cuts at: 6832 826 Size: 1471 Fragments arranged by size: 1471 1229 1136 1135 961 681 454 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7477 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7477 SmaI CCC'GGG Cuts at: 1872 4643 4804 1872 Size: 2771 161 4545 Fragments arranged by size: 4545 2771 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 3352 5581 6482 6744 820 Size: 197 926 1409 2229 901 262 1553 Fragments arranged by size: 2229 1553 1409 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4774 5268 493 Size: 4281 494 2702 Fragments arranged by size: 4281 2702 494 SpeI A'CTAG_T Cuts at: 152 3880 152 Size: 3728 3749 SphI G_CATG'C Cuts at: 1132 4835 1132 Size: 3703 3774 SspI AAT'ATT Cuts at: 5 52 5401 5 Size: 47 5349 2081 Fragments arranged by size: 5349 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7477 StyI C'CwwG_G Cuts at: 513 1065 2022 2237 2466 2638 3378 3414 4362 Size: 552 957 215 229 172 740 36 948 Cuts at: 4362 4916 513 Size: 554 3074 Fragments arranged by size: 3074 957 948 740 554 552 229 215 172 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2505 2723 5504 5521 5674 5859 6304 Size: 1255 925 218 2781 17 153 185 445 Cuts at: 6304 325 Size: 1498 Fragments arranged by size: 2781 1498 1255 925 445 218 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3074 3136 3663 Size: 274 80 33 51 1443 1097 62 527 Cuts at: 3663 3942 3994 4206 4510 5723 7435 96 Size: 279 52 212 304 1213 1712 138 Fragments arranged by size: 1712 1443 1213 1097 527 304 279 274 212 138 80 62 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2154 2186 2719 2745 2751 2777 7437 7469 2154 Size: 32 533 26 6 26 4660 32 2162 Fragments arranged by size: 4660 2162 533 32 32 26 26 6 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7477 XbaI T'CTAG_A Cuts at: 4813 4813 Size: 7477 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3421 4600 3421 Size: 1179 6298 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7477 XmaI C'CCGG_G Cuts at: 1870 4641 4802 1870 Size: 2771 161 4545 Fragments arranged by size: 4545 2771 161 XmnI GAAnn'nnTTC Cuts at: 2742 4734 5606 2742 Size: 1992 872 4613 Fragments arranged by size: 4613 1992 872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5285 276 Size: 53 83 186 936 3751 2468 Fragments arranged by size: 3751 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI