Restriction map for
pFN21AA1204
(Circular) (Six-base) MAPSORT of: pFN21AA1204.seq Check: 8338 from: 1 to: 9037
pFN21AA1204 9037 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2599 4360 6090 2599
Size: 1761 1730 5546
Fragments arranged by size:
5546 1761 1730
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6847 278
Size: 53 83 186 936 5311 2468
Fragments arranged by size:
5311 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6380 1937
Size: 4443 4594
Acc65I G'GTAC_C
Cuts at: 6358 6358
Size: 9037
AclI AA'CG_TT
Cuts at: 1563 7164 7537 1563
Size: 5601 373 3063
Fragments arranged by size:
5601 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2394 2464 3320 3717 4055 4490 4644 4790
Size: 762 70 856 397 338 435 154 146
Cuts at: 4790 4949 5008 7100 8484 8752 1632
Size: 159 59 2092 1384 268 1917
Fragments arranged by size:
2092 1917 1384 856 762 435 397 338
268 159 154 146 70 59
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8840
AflIII A'CryG_T
Cuts at: 1203 7936 8913 1203
Size: 6733 977 1327
Fragments arranged by size:
6733 1327 977
AgeI A'CCGG_T
Cuts at: 3113 7868 3113
Size: 4755 4282
AhdI GACnn_n'nnGTC
Cuts at: 7766 7766
Size: 9037
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9037
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2088 2120 2088
Size: 32 9005
AlwNI CAG_nnn'CTG
Cuts at: 2032 3065 3653 3695 3982 4237 4926 5497 5543
Size: 1033 588 42 287 255 689 571 46
Cuts at: 5543 5650 5689 6780 8352 2032
Size: 107 39 1091 1572 2717
Fragments arranged by size:
2717 1572 1091 1033 689 588 571 287
255 107 46 42 39
ApaI G_GGCC'C
Cuts at: 4080 5295 4080
Size: 1215 7822
ApaLI G'TGCA_C
Cuts at: 2661 7094 8250 2661
Size: 4433 1156 3448
Fragments arranged by size:
4433 3448 1156
ApoI r'AATT_y
Cuts at: 1493 2132 2345 3091 4546 4951 6346 6572 6724
Size: 639 213 746 1455 405 1395 226 152
Cuts at: 6724 8833 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1455 1395 746 639 405 226
213 152
AseI AT'TA_AT
Cuts at: 160 1032 7591 160
Size: 872 6559 1606
Fragments arranged by size:
6559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9037
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 3218 4061 4904 6362 1870
Size: 73 149 1126 843 843 1458 4545
Fragments arranged by size:
4545 1458 1126 843 843 149 73
BamHI G'GATC_C
Cuts at: 3322 4165 6367 6734 3322
Size: 843 2202 367 5625
Fragments arranged by size:
5625 2202 843 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2527 2846 3155 3746 4975
Size: 325 217 627 740 319 309 591 1229
Cuts at: 4975 6358 7813 618
Size: 1383 1455 1842
Fragments arranged by size:
1842 1455 1383 1229 740 627 591 325
319 309 217
BanII G_rGCy'C
Cuts at: 1391 2282 2337 3429 3471 3791 3893 4011 4080
Size: 891 55 1092 42 320 102 118 69
Cuts at: 4080 5295 5666 6356 1391
Size: 1215 371 690 4072
Fragments arranged by size:
4072 1215 1092 891 690 371 320 118
102 69 55 42
BbeI G_GCGC'C
Cuts at: 2531 3750 2531
Size: 1219 7818
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3391 4043 4431 6037 928
Size: 971 1492 652 388 1606 3928
Fragments arranged by size:
3928 1606 1492 971 652 388
BbvCI CC'TCA_GC
Cuts at: 3225 6288 3225
Size: 3063 5974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3455 3489 3532 3566 7228 7262 1903
Size: 34 1518 34 43 34 3662 34 3678
Fragments arranged by size:
3678 3662 1518 43 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6929 8139 6929
Size: 1210 7827
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9037
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4259 7648 136
Size: 107 122 71 631 605 2587 3389 1525
Fragments arranged by size:
3389 2587 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2236 4450 4491 9032 2236
Size: 2214 41 4541 2241
Fragments arranged by size:
4541 2241 2214 41
BlpI GC'TnA_GC
Cuts at: 2022 6454 2022
Size: 4432 4605
Bme1580I G_kGCm'C
Cuts at: 1790 2665 2849 4080 5160 5295 7098 8254 1790
Size: 875 184 1231 1080 135 1803 1156 2573
Fragments arranged by size:
2573 1803 1231 1156 1080 875 184 135
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9037
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4732 5600 6768
Size: 416 53 83 399 24 3315 868 1168
Cuts at: 6768 7726 442
Size: 958 1753
Fragments arranged by size:
3315 1753 1168 958 868 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 4027 4633 4881 4930 2236
Size: 1791 606 248 49 6343
Fragments arranged by size:
6343 1791 606 248 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2696 2881 3248 3776 4116 4169 4374
Size: 1004 453 185 367 528 340 53 205
Cuts at: 4374 4397 4730 4903 5268 5488 5711 5856 7697
Size: 23 333 173 365 220 223 145 1841
Cuts at: 7697 1239
Size: 2579
Fragments arranged by size:
2579 1841 1004 528 453 367 365 340
333 223 220 205 185 173 145 53
23
Bpu10I CC'TnA_GC
Cuts at: 2204 3225 3273 4227 5665 6288 2204
Size: 1021 48 954 1438 623 4953
Fragments arranged by size:
4953 1438 1021 954 623 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2748 2774 2888 3695 3805 7162 8027 8325 2748
Size: 26 114 807 110 3357 865 298 3460
Fragments arranged by size:
3460 3357 865 807 298 114 110 26
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2676 5410 5412 6254 7700 882
Size: 617 1177 2734 2 842 1446 2219
Fragments arranged by size:
2734 2219 1446 1177 842 617 2
BsaAI yAC'GTr
Cuts at: 493 1204 6828 493
Size: 711 5624 2702
Fragments arranged by size:
5624 2702 711
BsaBI GATnn'nnATC
Cuts at: 9031 9031
Size: 9037
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2528 3747 6844 7226
Size: 53 83 186 936 995 1219 3097 382
Cuts at: 7226 7861 275
Size: 635 1451
Fragments arranged by size:
3097 1451 1219 995 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3113 3289 3325 3826 7470 7868 8142 8289 8700
Size: 176 36 501 3644 398 274 147 411
Cuts at: 8700 3113
Size: 3450
Fragments arranged by size:
3644 3450 501 411 398 274 176 147
36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2345 2375 3539 3569 5854
Size: 30 165 30 942 30 1164 30 2285
Cuts at: 5854 5882 5884 5912 1178
Size: 28 2 28 4303
Fragments arranged by size:
4303 2285 1164 942 165 30 30 30
30 28 28 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2528 3773 3857 4016 4019 4254 4329 4347
Size: 1342 1245 84 159 3 235 75 18
Cuts at: 4347 4362 4394 4529 4643 4906 5049 5710 5752
Size: 15 32 135 114 263 143 661 42
Cuts at: 5752 1186
Size: 4471
Fragments arranged by size:
4471 1342 1245 661 263 235 159 143
135 114 84 75 42 32 18 15
3
BseYI C'CCAG_C
Cuts at: 3628 4698 4844 5098 5222 5711 8240 8736 3628
Size: 1070 146 254 124 489 2529 496 3929
Fragments arranged by size:
3929 2529 1070 496 489 254 146 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2148 3550 4223 5249 5823 1163
Size: 573 412 1402 673 1026 574 4377
Fragments arranged by size:
4377 1402 1026 673 574 573 412
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6487 7248 8276 1540
Size: 275 186 4486 761 1028 2301
Fragments arranged by size:
4486 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 2665 3791 4011 4184 5256 5282 5666 7098
Size: 328 1126 220 173 1072 26 384 1432
Cuts at: 7098 7183 8254 2337
Size: 85 1071 3120
Fragments arranged by size:
3120 1432 1126 1072 1071 384 328 220
173 85 26
BsmI GAATG_Cn'
Cuts at: 3722 4561 5403 6548 6641 3722
Size: 839 842 1145 93 6118
Fragments arranged by size:
6118 1145 842 839 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4621 4621
Size: 9037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2282 2337 2665 2849 3429 3471 3791
Size: 399 492 55 328 184 580 42 320
Cuts at: 3791 3893 4011 4080 4184 5160 5256 5282 5295
Size: 102 118 69 104 976 96 26 13
Cuts at: 5295 5666 6356 7098 7183 8254 1391
Size: 371 690 742 85 1071 2174
Fragments arranged by size:
2174 1071 976 742 690 580 492 399
371 328 320 184 118 104 102 96
85 69 55 42 26 13
BspEI T'CCGG_A
Cuts at: 3325 3826 8700 3325
Size: 501 4874 3662
Fragments arranged by size:
4874 3662 501
BspHI T'CATG_A
Cuts at: 2700 6926 8656 2700
Size: 4226 1730 3081
Fragments arranged by size:
4226 3081 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2599 3659 4360 5392 5749 5901 6090 6392
Size: 1755 1060 701 1032 357 152 189 302
Cuts at: 6392 844
Size: 3489
Fragments arranged by size:
3489 1755 1060 1032 701 357 302 189
152
BsrBI CCG'CTC
Cuts at: 1394 1823 3490 3600 6924 1394
Size: 429 1667 110 3324 3507
Fragments arranged by size:
3507 3324 1667 429 110
BsrDI GCAATG_nn'
Cuts at: 65 1248 2365 2578 4692 5346 5927 7532 7706
Size: 1183 1117 213 2114 654 581 1605 174
Cuts at: 7706 65
Size: 1396
Fragments arranged by size:
2114 1605 1396 1183 1117 654 581 213
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2691 3113 3152 3158 7681 7868 1700
Size: 110 881 422 39 6 4523 187 2869
Fragments arranged by size:
4523 2869 881 422 187 110 39 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9037
BssSI C'ACGA_G
Cuts at: 7097 8109 7097
Size: 1012 8025
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3528 4237 794
Size: 2734 709 5594
Fragments arranged by size:
5594 2734 709
BstBI TT'CG_AA
Cuts at: 3374 7841 3374
Size: 4467 4570
BstEII G'GTnAC_C
Cuts at: 1184 4811 1184
Size: 3627 5410
BstXI CCAn_nnnn'nTGG
Cuts at: 2743 4147 2743
Size: 1404 7633
BstYI r'GATC_y
Cuts at: 1972 2236 3322 4165 4450 4491 5637 5800 6367
Size: 264 1086 843 285 41 1146 163 567
Cuts at: 6367 6734 7119 7136 8588 8674 8686 9032 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1146 1086 843 567 385 367
346 285 264 163 86 41 17 12
Bsu36I CC'TnA_GG
Cuts at: 2540 5015 2540
Size: 2475 6562
BtgI C'CryG_G
Cuts at: 513 1065 2841 4881 513
Size: 552 1776 2040 4669
Fragments arranged by size:
4669 2040 1776 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8298
Fragments arranged by size:
8298 683 56
BtsI GCAGTG_nn'
Cuts at: 2364 3253 5835 6553 7347 7367 2364
Size: 889 2582 718 794 20 4034
Fragments arranged by size:
4034 2582 889 794 718 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9002
DraI TTT'AAA
Cuts at: 3801 6341 3801
Size: 2540 6497
DraIII CAC_nnn'GTG
Cuts at: 2275 3528 5286 2275
Size: 1253 1758 6026
Fragments arranged by size:
6026 1758 1253
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5426 8044 809
Size: 507 4110 2618 1802
Fragments arranged by size:
4110 2618 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2778 3608 3945
Size: 54 1425 50 275 18 948 830 337
Cuts at: 3945 6247 6484 7373 9002 8
Size: 2302 237 889 1629 43
Fragments arranged by size:
2302 1629 1425 948 889 830 337 275
237 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6484 1537
Size: 275 4672 4090
Fragments arranged by size:
4672 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2699 2858 3419 3567 3612 3809 4620
Size: 761 585 159 561 148 45 197 811
Cuts at: 4620 4820 4968 5363 6967 1353
Size: 200 148 395 1604 3423
Fragments arranged by size:
3423 1604 811 761 585 561 395 200
197 159 148 148 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7624 7998 8144 387
Size: 1064 138 6035 374 146 1280
Fragments arranged by size:
6035 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 3789 4009 5664 2335
Size: 1454 220 1655 5708
Fragments arranged by size:
5708 1655 1454 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2394 2464 2696 2881 3248 3320
Size: 393 611 151 70 232 185 367 72
Cuts at: 3320 3717 3776 4055 4116 4169 4374 4397 4490
Size: 397 59 279 61 53 205 23 93
Cuts at: 4490 4644 4730 4790 4903 4949 5008 5268 5488
Size: 154 86 60 113 46 59 260 220
Cuts at: 5488 5711 5856 7100 7697 8484 8752 1239
Size: 223 145 1244 597 787 268 1524
Fragments arranged by size:
1524 1244 787 611 597 397 393 367
279 268 260 232 223 220 205 185
154 151 145 113 93 86 72 70
61 60 59 59 53 46 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2376 3069 3734 4505 5126 2376
Size: 693 665 771 621 6287
Fragments arranged by size:
6287 771 693 665 621
EcoO109I rG'GnC_Cy
Cuts at: 2436 4076 4077 4234 4801 5019 5292 5485 5494
Size: 1640 1 157 567 218 273 193 9
Cuts at: 5494 5708 5858 6115 2436
Size: 214 150 257 5358
Fragments arranged by size:
5358 1640 567 273 257 218 214 193
157 150 9 1
EcoRI G'AATT_C
Cuts at: 4951 6346 6724 4951
Size: 1395 378 7264
Fragments arranged by size:
7264 1395 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 3932 2314
Size: 1618 7419
FspI TGC'GCA
Cuts at: 7543 7543
Size: 9037
HaeII r_GCGC'y
Cuts at: 1678 2531 3273 3455 3750 8184 1678
Size: 853 742 182 295 4434 2531
Fragments arranged by size:
4434 2531 853 742 295 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2345 2377 2821
Size: 32 135 32 493 32 248 32 444
Cuts at: 2821 2853 2942 2974 2988 3020 3235 3267 3539
Size: 32 89 32 14 32 215 32 272
Cuts at: 3539 3571 3808 3840 3854 3886 3998 4030 4150
Size: 32 237 32 14 32 112 32 120
Cuts at: 4150 4182 4277 4309 4435 4467 5744 5776 5975
Size: 32 95 32 126 32 1277 32 199
Cuts at: 5975 6007 7678 7710 7752 7784 1373
Size: 32 1671 32 42 32 2626
Fragments arranged by size:
2626 1671 1277 493 444 272 248 237
215 199 135 126 120 112 95 89
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4386 6381 6627 669
Size: 988 281 2448 1995 246 3079
Fragments arranged by size:
3079 2448 1995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9037
HpaI GTT'AAC
Cuts at: 6627 6627
Size: 9037
KasI G'GCGC_C
Cuts at: 2527 3746 2527
Size: 1219 7818
KpnI G_GTAC'C
Cuts at: 6362 6362
Size: 9037
MfeI C'AATT_G
Cuts at: 6636 6636
Size: 9037
MluI A'CGCG_T
Cuts at: 8913 8913
Size: 9037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3052 4391 4800 8151 8335 1305
Size: 1747 1339 409 3351 184 2007
Fragments arranged by size:
3351 2007 1747 1339 409 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2780 3610 3947 6249 10
Size: 54 1425 343 948 830 337 2302 2798
Fragments arranged by size:
2798 2302 1425 948 830 343 337 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3801 5435 6995 7354 7513 518
Size: 648 2635 1634 1560 359 159 2042
Fragments arranged by size:
2635 2042 1634 1560 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3298 3628 4468 4926 5558 6273 6292 6453
Size: 1692 330 840 458 632 715 19 161
Cuts at: 6453 7130 8278 8523 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1692 1148 840 715 677 632 458
330 245 161 19
NarI GG'CG_CC
Cuts at: 2528 3747 2528
Size: 1219 7818
NcoI C'CATG_G
Cuts at: 513 1065 2841 513
Size: 552 1776 6709
Fragments arranged by size:
6709 1776 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9037
NotI GC'GGCC_GC
Cuts at: 6484 6484
Size: 9037
NspI r_CATG'y
Cuts at: 1132 1229 2500 5851 5938 6395 7940 1132
Size: 97 1271 3351 87 457 1545 2229
Fragments arranged by size:
3351 2229 1545 1271 457 97 87
PasI CC'CwG_GG
Cuts at: 1348 3894 4725 1348
Size: 2546 831 5660
Fragments arranged by size:
5660 2546 831
PciI A'CATG_T
Cuts at: 7936 7936
Size: 9037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 2611 3928 5497 1108
Size: 1167 336 1317 1569 4648
Fragments arranged by size:
4648 1569 1317 1167 336
PfoI T'CCnGG_A
Cuts at: 2259 2409 4433 5803 2259
Size: 150 2024 1370 5493
Fragments arranged by size:
5493 2024 1370 150
PmeI GTTT'AAAC
Cuts at: 6341 6341
Size: 9037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7106 7138 8619 8651 7106
Size: 32 1481 32 7492
Fragments arranged by size:
7492 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2436 5019 5485 5494 5708 5858 6115 2436
Size: 2583 466 9 214 150 257 5358
Fragments arranged by size:
5358 2583 466 257 214 150 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9037
PsiI TTA'TAA
Cuts at: 6607 6607
Size: 9037
PspOMI G'GGCC_C
Cuts at: 4076 5291 4076
Size: 1215 7822
PstI C_TGCA'G
Cuts at: 830 3555 3649 3885 6389 830
Size: 2725 94 236 2504 3478
Fragments arranged by size:
3478 2725 2504 236 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9037
PvuII CAG'CTG
Cuts at: 3298 4468 4926 5558 6273 3298
Size: 1170 458 632 715 6062
Fragments arranged by size:
6062 1170 715 632 458
SacI G_AGCT'C
Cuts at: 2337 3791 4011 5666 2337
Size: 1454 220 1655 5708
Fragments arranged by size:
5708 1655 1454 220
SalI G'TCGA_C
Cuts at: 1936 6379 1936
Size: 4443 4594
SanDI GG'GwC_CC
Cuts at: 5708 5708
Size: 9037
SapI GCTCTTCn'nnn_
Cuts at: 2114 2699 2114
Size: 585 8452
SbfI CC_TGCA'GG
Cuts at: 3885 6389 3885
Size: 2504 6533
ScaI AGT'ACT
Cuts at: 7285 8997 7285
Size: 1712 7325
SfcI C'TryA_G
Cuts at: 826 1045 2431 3551 3645 3881 6385 7520 8201
Size: 219 1386 1120 94 236 2504 1135 681
Cuts at: 8201 8392 826
Size: 191 1471
Fragments arranged by size:
2504 1471 1386 1135 1120 681 236 219
191 94
SfoI GGC'GCC
Cuts at: 2529 3748 2529
Size: 1219 7818
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9037
SgrAI Cr'CCGG_yG
Cuts at: 1700 3113 1700
Size: 1413 7624
SmaI CCC'GGG
Cuts at: 1872 3220 6364 1872
Size: 1348 3144 4545
Fragments arranged by size:
4545 3144 1348
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2753 2763 2867 3674 3784 4061
Size: 197 926 810 10 104 807 110 277
Cuts at: 4061 4904 7141 8042 8304 820
Size: 843 2237 901 262 1553
Fragments arranged by size:
2237 1553 926 901 843 810 807 277
262 197 110 104 10
SnaBI TAC'GTA
Cuts at: 493 6828 493
Size: 6335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9037
SphI G_CATG'C
Cuts at: 1132 6395 1132
Size: 5263 3774
SspI AAT'ATT
Cuts at: 5 52 2925 6961 5
Size: 47 2873 4036 2081
Fragments arranged by size:
4036 2873 2081 47
StuI AGG'CCT
Cuts at: 1517 3729 5314 1517
Size: 2212 1585 5240
Fragments arranged by size:
5240 2212 1585
StyI C'CwwG_G
Cuts at: 513 1065 2439 2841 3084 3684 6111 6476 513
Size: 552 1374 402 243 600 2427 365 3074
Fragments arranged by size:
3074 2427 1374 600 552 402 365 243
SwaI ATTT'AAAT
Cuts at: 3801 3801
Size: 9037
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4257 4732 4993 5236 5648 5918 6158
Size: 1255 2677 475 261 243 412 270 240
Cuts at: 6158 7064 7081 7234 7419 7864 325
Size: 906 17 153 185 445 1498
Fragments arranged by size:
2677 1498 1255 906 475 445 412 270
261 243 240 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4648 5628 7283
Size: 274 80 33 51 1443 2671 980 1655
Cuts at: 7283 8995 96
Size: 1712 138
Fragments arranged by size:
2671 1712 1655 1443 980 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2773 2805 4564 4596 4724 4756 8997 9029 2773
Size: 32 1759 32 128 32 4241 32 2781
Fragments arranged by size:
4241 2781 1759 128 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9037
XbaI T'CTAG_A
Cuts at: 4867 6373 4867
Size: 1506 7531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5430 5430
Size: 9037
XhoI C'TCGA_G
Cuts at: 1943 4061 4904 1943
Size: 2118 843 6076
Fragments arranged by size:
6076 2118 843
XmaI C'CCGG_G
Cuts at: 1870 3218 6362 1870
Size: 1348 3144 4545
Fragments arranged by size:
4545 3144 1348
XmnI GAAnn'nnTTC
Cuts at: 3305 4275 7166 3305
Size: 970 2891 5176
Fragments arranged by size:
5176 2891 970
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6845 276
Size: 53 83 186 936 5311 2468
Fragments arranged by size:
5311 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SexAI SfiI SrfI