Restriction map for
pFN21AA0672
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BciVI BclI BfrI BlnI
BsaBI BsiWI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
MamI MluI MseI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SapI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 1931 TCCGGA T^CCGGA
AcsI 1147 AAATTT [GA]^AATT[CT]
AflIII 1383 ACACGT A^C[GA][CT]GT
Aor51HI 191 AGCGCT AGC^GCT
ApaI 1747 GGGCCC GGGCC^C
ApoI 1147 AAATTT [GA]^AATT[CT]
BamHI 1040 GGATCC G^GATCC
BbrPI 1384 CACGTG CAC^GTG
BglI 1648 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2316 AGATCT A^GATCT
BlpI 1870 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1870 GCTCAGC GC^T[ACGT]AGC
BsaAI 1384 CACGTG [CT]AC^GT[GA]
BsaI 2202 GAGACC GGTCTC(1/5)
BsaWI 1931 TCCGGA [AT]^CCGG[AT]
BseAI 1931 TCCGGA T^CCGGA
BsmI 1092 GAATGC GAATGC(1/-1)
Bsp1407I 602 TGTACA T^GTACA
BspEI 1931 TCCGGA T^CCGGA
BsrGI 602 TGTACA T^GTACA
CelII 1870 GCTCAGC GC^T[ACGT]AGC
Eco47III 191 AGCGCT AGC^GCT
EcoNI 84 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 2361 GTCGAC GT[CT]^[GA]AC
HindII 2361 GTCGAC GT[CT]^[GA]AC
HindIII 1075 AAGCTT A^AGCTT
KspI 1703 CCGCGG CCGC^GG
MroI 1931 TCCGGA T^CCGGA
MslI 1542 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1466 GCCGGC GCC^GGC
NgoMIV 1466 GCCGGC G^CCGGC
PmaCI 1384 CACGTG CAC^GTG
PmlI 1384 CACGTG CAC^GTG
PshAI 1912 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1703 CCGCGG CCGC^GG
SalI 2361 GTCGAC G^TCGAC
ScaI 944 AGTACT AGT^ACT
SexAI 460 ACCAGGT A^CC[AT]GGT
SmlI 838 CTTGAG C^T[CT][GA]AG
SspBI 602 TGTACA T^GTACA
SspI 400 AATATT AAT^ATT
StuI 734 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2310 GTCTAC GT^[AC][GT]AC
2361 GTCGAC
AcyI 1445 GGCGCC G[GA]^CG[CT]C
1879 GGCGCC
Asp700I 6 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
79 GAAGACCTTC
BbeI 1445 GGCGCC GGCGC^C
1879 GGCGCC
BbsI 79 GAAGAC GAAGAC(2/6)
483 GTCTTC
BbvCI 1473 CCTCAGC CCTCAGC(-5/-2)
2221 CCTCAGC
BcgI 1650 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
9 GCAGTTCAATCG
BfaI 2252 CTAG C^TAG
2281 CTAG
BpuAI 79 GAAGAC GAAGAC(2/6)
483 GTCTTC
BsaHI 1445 GGCGCC G[GA]^CG[CT]C
1879 GGCGCC
BsmBI 298 GAGACG CGTCTC(1/5)
353 GAGACG
BstYI 1040 GGATCC [GA]^GATC[CT]
2316 AGATCT
DrdI 474 GACTTCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1269 GACCACAGTGTC
EagI 792 CGGCCG C^GGCCG
1592 CGGCCG
EclXI 792 CGGCCG C^GGCCG
1592 CGGCCG
Eco52I 792 CGGCCG C^GGCCG
1592 CGGCCG
Hin1I 1445 GGCGCC G[GA]^CG[CT]C
1879 GGCGCC
KasI 1445 GGCGCC G^GCGCC
1879 GGCGCC
MaeI 2252 CTAG C^TAG
2281 CTAG
MaeII 356 ACGT A^CGT
1385 ACGT
MfeI 965 CAATTG C^AATTG
1208 CAATTG
MflI 1040 GGATCC [GA]^GATC[CT]
2316 AGATCT
MunI 965 CAATTG C^AATTG
1208 CAATTG
NarI 1445 GGCGCC GG^CGCC
1879 GGCGCC
PflMI 39 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1536 CCAAAGCATGG
PpuMI 1218 AGGACCC [GA]G^G[AT]CC[CT]
1970 AGGTCCC
SacI 1868 GAGCTC GAGCT^C
2353 GAGCTC
SfoI 1445 GGCGCC GGC^GCC
1879 GGCGCC
SphI 20 GCATGC GCATG^C
1317 GCATGC
TfiI 448 GATTC G^A[AT]TC
1959 GATTC
Tsp45I 826 GTGAC ^GT[GC]AC
2061 GTCAC
Van91I 39 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1536 CCAAAGCATGG
XcmI 35 CCAACCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
428 CCAAGTTGGTGCTGG
XhoII 1040 GGATCC [GA]^GATC[CT]
2316 AGATCT
XmnI 6 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
79 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp509I TspRI TthHB8I