Restriction map for pFN21AA0546
 (Linear) (Six-base) MAPSORT of: KIAA0546.seq  Check: 7031  from: 1  to: 5730



KIAA0546.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1164    5730 
   Size:       1164    4566
 
AccI GT'mk_AC

Cuts at:      0    3925    5730 
   Size:       3925    1805
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1120    1400    1490    1838    2333    2416    2612    2659 
   Size:       1120     280      90     348     495      83     196      47

Cuts at:   2659    3098    3701    3888    5730 
   Size:        439     603     187    1842

  Fragments arranged by size: 

                1842    1120     603     495     439     348     280     196
                 187      90      83      47
 
AflII C'TTAA_G

Cuts at:      0    3172    5730 
   Size:       3172    2558
 
AflIII A'CryG_T

Cuts at:      0     272    3577    4721    5730 
   Size:        272    3305    1144    1009

  Fragments arranged by size: 

                3305    1144    1009     272
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3654    5730 
   Size:       3654    2076
 
AlwNI CAG_nnn'CTG

Cuts at:      0     246    1513    4914    5461    5667    5730 
   Size:        246    1267    3401     547     206      63

  Fragments arranged by size: 

                3401    1267     547     246     206      63
 
ApaLI G'TGCA_C

Cuts at:      0    4487    5730 
   Size:       4487    1243
 
ApoI r'AATT_y

Cuts at:      0     252    1035    1421    2544    2639    2684    2936    3016 
   Size:        252     783     386    1123      95      45     252      80

Cuts at:   3016    3101    4205    4600    4621    5032    5083    5199    5413 
   Size:         85    1104     395      21     411      51     116     214

Cuts at:   5413    5419    5512    5730 
   Size:          6      93     218

  Fragments arranged by size: 

                1123    1104     783     411     395     386     252     252
                 218     214     116      95      93      85      80      51
                  45      21       6
 
AvaI C'yCGr_G

Cuts at:      0     182    2753    5730 
   Size:        182    2571    2977

  Fragments arranged by size: 

                2977    2571     182
 
AvrII C'CTAG_G

Cuts at:      0    2041    5730 
   Size:       2041    3689
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3977    4010    5730 
   Size:       3977      33    1720

  Fragments arranged by size: 

                3977    1720      33
 
BamHI G'GATC_C

Cuts at:      0     541    5392    5730 
   Size:        541    4851     338

  Fragments arranged by size: 

                4851     541     338
 
BanI G'GyrC_C

Cuts at:      0    4211    5730 
   Size:       4211    1519
 
BanII G_rGCy'C

Cuts at:      0     367     578     598    3514    5730 
   Size:        367     211      20    2916    2216

  Fragments arranged by size: 

                2916    2216     367     211      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2071    2404    5730 
   Size:       2071     333    3326

  Fragments arranged by size: 

                3326    2071     333
 
BbvCI CC'TCA_GC

Cuts at:      0     247    5730 
   Size:        247    5483
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1593    2039    3978    5730 
   Size:       1593     446    1939    1752

  Fragments arranged by size: 

                1939    1752    1593     446
 
BfrBI ATG'CAT

Cuts at:      0     722    3203    4510    5730 
   Size:        722    2481    1307    1220

  Fragments arranged by size: 

                2481    1307    1220     722
 
BglII A'GATC_T

Cuts at:      0    1428    3133    5730 
   Size:       1428    1705    2597

  Fragments arranged by size: 

                2597    1705    1428
 
BlpI GC'TnA_GC

Cuts at:      0    2234    5730 
   Size:       2234    3496
 
Bme1580I G_kGCm'C

Cuts at:      0    2964    4491    5730 
   Size:       2964    1527    1239

  Fragments arranged by size: 

                2964    1527    1239
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     489    1607    1667    1952    2831    2978    3983 
   Size:         15     474    1118      60     285     879     147    1005

Cuts at:   3983    4277    4864    5730 
   Size:        294     587     866

  Fragments arranged by size: 

                1118    1005     879     866     587     474     294     285
                 147      60      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     247    3508    5730 
   Size:        247    3261    2222

  Fragments arranged by size: 

                3261    2222     247
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4078    5730 
   Size:       4078    1652
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     338     620    5730 
   Size:        338     282    5110

  Fragments arranged by size: 

                5110     338     282
 
BsaAI yAC'GTr

Cuts at:      0    4356    5730 
   Size:       4356    1374
 
BsaWI w'CCGG_w

Cuts at:      0     329    2298    3540    5730 
   Size:        329    1969    1242    2190

  Fragments arranged by size: 

                2190    1969    1242     329
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1605    1635    1793    1823    5730 
   Size:       1605      30     158      30    3907

  Fragments arranged by size: 

                3907    1605     158      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     161     497     512     545     548     877    1500    1571 
   Size:        161     336      15      33       3     329     623      71

Cuts at:   1571    1778    1879    3818    5730 
   Size:        207     101    1939    1912

  Fragments arranged by size: 

                1939    1912     623     336     329     207     161     101
                  71      33      15       3
 
BseYI C'CCAG_C

Cuts at:      0     301    2415    5561    5730 
   Size:        301    2114    3146     169

  Fragments arranged by size: 

                3146    2114     301     169
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     534     790    3704    4279    4444    4816    5730 
   Size:        534     256    2914     575     165     372     914

  Fragments arranged by size: 

                2914     914     575     534     372     256     165
 
BsiEI CG_ry'CG

Cuts at:      0       8     391    5730 
   Size:          8     383    5339

  Fragments arranged by size: 

                5339     383       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     367     598    3514    4138    4491    5730 
   Size:        367     231    2916     624     353    1239

  Fragments arranged by size: 

                2916    1239     624     367     353     231
 
BsmI GAATG_Cn'

Cuts at:      0     724    2868    3205    4512    5010    5565    5730 
   Size:        724    2144     337    1307     498     555     165

  Fragments arranged by size: 

                2144    1307     724     555     498     337     165
 
Bsp1286I G_dGCh'C

Cuts at:      0     367     578     598    2964    3514    4138    4491    5730 
   Size:        367     211      20    2366     550     624     353    1239

  Fragments arranged by size: 

                2366    1239     624     550     367     353     211      20
            
 
BspHI T'CATG_A

Cuts at:      0    1463    3042    5730 
   Size:       1463    1579    2688

  Fragments arranged by size: 

                2688    1579    1463
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1164    2463    5730 
   Size:       1164    1299    3267

  Fragments arranged by size: 

                3267    1299    1164
 
BsrBI CCG'CTC

Cuts at:      0     282     405    5730 
   Size:        282     123    5325

  Fragments arranged by size: 

                5325     282     123
 
BsrDI GCAATG_nn'

Cuts at:      0    2465    4868    5661    5730 
   Size:       2465    2403     793      69

  Fragments arranged by size: 

                2465    2403     793      69
 
BsrGI T'GTAC_A

Cuts at:      0    1218    4080    5730 
   Size:       1218    2862    1650

  Fragments arranged by size: 

                2862    1650    1218
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3875    4208    5577    5730 
   Size:       3875     333    1369     153

  Fragments arranged by size: 

                3875    1369     333     153
 
BstYI r'GATC_y

Cuts at:      0     193     541     777    1428    1509    1821    3133    5133 
   Size:        193     348     236     651      81     312    1312    2000

Cuts at:   5133    5392    5730 
   Size:        259     338

  Fragments arranged by size: 

                2000    1312     651     348     338     312     259     236
                 193      81
 
Bsu36I CC'TnA_GG

Cuts at:      0     292     860    1519    1715    5730 
   Size:        292     568     659     196    4015

  Fragments arranged by size: 

                4015     659     568     292     196
 
BtgI C'CryG_G

Cuts at:      0     157    4684    5730 
   Size:        157    4527    1046

  Fragments arranged by size: 

                4527    1046     157
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    4023    5730 
   Size:       4023    1707
 
BtsI GCAGTG_nn'

Cuts at:      0    2771    4754    4976    5493    5730 
   Size:       2771    1983     222     517     237

  Fragments arranged by size: 

                2771    1983     517     237     222
 
ClaI AT'CG_AT

Cuts at:      0    1458    4563    5730 
   Size:       1458    3105    1167

  Fragments arranged by size: 

                3105    1458    1167
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2747    2782    5730 
   Size:       2747      35    2948

  Fragments arranged by size: 

                2948    2747      35
 
DraI TTT'AAA

Cuts at:      0     689    2540    3317    4160    5103    5730 
   Size:        689    1851     777     843     943     627

  Fragments arranged by size: 

                1851     943     843     777     689     627
 
EaeI y'GGCC_r

Cuts at:      0     388    1256    2768    5714    5730 
   Size:        388     868    1512    2946      16

  Fragments arranged by size: 

                2946    1512     868     388      16
 
EagI C'GGCC_G

Cuts at:      0     388    5730 
   Size:        388    5342
 
EarI CTCTTCn'nnn_

Cuts at:      0     219     237     586     755    1091    1139    1352    1382 
   Size:        219      18     349     169     336      48     213      30

Cuts at:   1382    1856    2342    2738    2999    3452    5273    5730 
   Size:        474     486     396     261     453    1821     457

  Fragments arranged by size: 

                1821     486     474     457     453     396     349     336
                 261     219     213     169      48      30      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     215     329    5730 
   Size:        215     114    5401

  Fragments arranged by size: 

                5401     215     114
 
EcoICRI GAG'CTC

Cuts at:      0     365     596    3512    5730 
   Size:        365     231    2916    2218

  Fragments arranged by size: 

                2916    2218     365     231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     489    1120    1400    1490    1607    1667    1838 
   Size:         15     474     631     280      90     117      60     171

Cuts at:   1838    1952    2333    2416    2612    2659    2831    2978    3098 
   Size:        114     381      83     196      47     172     147     120

Cuts at:   3098    3701    3888    3983    4277    4864    5730 
   Size:        603     187      95     294     587     866

  Fragments arranged by size: 

                 866     631     603     587     474     381     294     280
                 196     187     172     171     147     120     117     114
                  95      90      83      60      47      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     527    5730 
   Size:        527    5203
 
EcoO109I rG'GnC_Cy

Cuts at:      0     298    2071    5730 
   Size:        298    1773    3659

  Fragments arranged by size: 

                3659    1773     298
 
EcoRI G'AATT_C

Cuts at:      0    4600    5413    5730 
   Size:       4600     813     317

  Fragments arranged by size: 

                4600     813     317
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      79     700    1520    1912    3152    5730 
   Size:         79     621     820     392    1240    2578

  Fragments arranged by size: 

                2578    1240     820     621     392      79
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      96     128    1054    1086    1090    1122    1924    1956 
   Size:         96      32     926      32       4      32     802      32

Cuts at:   1956    2037    2069    2137    2169    2473    2505    3640    3672 
   Size:         81      32      68      32     304      32    1135      32

Cuts at:   3672    3751    3783    3907    3939    5164    5196    5346    5378 
   Size:         79      32     124      32    1225      32     150      32

Cuts at:   5378    5485    5517    5730 
   Size:        107      32     213

  Fragments arranged by size: 

                1225    1135     926     802     304     213     150     124
                 107      96      81      79      68      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32       4
 
HincII GTy'rAC

Cuts at:      0    1202    2177    2652    4662    5730 
   Size:       1202     975     475    2010    1068

  Fragments arranged by size: 

                2010    1202    1068     975     475
 
HindIII A'AGCT_T

Cuts at:      0    2240    2671    2720    4096    4305    4754    5730 
   Size:       2240     431      49    1376     209     449     976

  Fragments arranged by size: 

                2240    1376     976     449     431     209      49
 
HpaI GTT'AAC

Cuts at:      0    2652    4662    5730 
   Size:       2652    2010    1068

  Fragments arranged by size: 

                2652    2010    1068
 
MfeI C'AATT_G

Cuts at:      0    5168    5730 
   Size:       5168     562
 
MluI A'CGCG_T

Cuts at:      0     272    5730 
   Size:        272    5458
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     689    1016    4352    5528    5730 
   Size:         94     595     327    3336    1176     202

  Fragments arranged by size: 

                3336    1176     595     327     202      94
 
MscI TGG'CCA

Cuts at:      0    2770    5716    5730 
   Size:       2770    2946      14

  Fragments arranged by size: 

                2946    2770      14
 
MslI CAynn'nnrTG

Cuts at:      0    4819    5730 
   Size:       4819     911
 
MspA1I CmG'CkG

Cuts at:      0     134     159     246    1368    2394    3309    4279    4458 
   Size:        134      25      87    1122    1026     915     970     179

Cuts at:   4458    5730 
   Size:       1272

  Fragments arranged by size: 

                1272    1122    1026     970     915     179     134      87
                  25
 
NcoI C'CATG_G

Cuts at:      0    4684    5730 
   Size:       4684    1046
 
NdeI CA'TA_TG

Cuts at:      0    5024    5301    5730 
   Size:       5024     277     429

  Fragments arranged by size: 

                5024     429     277
 
NsiI A_TGCA'T

Cuts at:      0     724    3205    4512    5730 
   Size:        724    2481    1307    1218

  Fragments arranged by size: 

                2481    1307    1218     724
 
NspI r_CATG'y

Cuts at:      0    3581    4725    5711    5730 
   Size:       3581    1144     986      19

  Fragments arranged by size: 

                3581    1144     986      19
 
PciI A'CATG_T

Cuts at:      0    3577    4721    5730 
   Size:       3577    1144    1009

  Fragments arranged by size: 

                3577    1144    1009
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4842    4914    5730 
   Size:       4842      72     816

  Fragments arranged by size: 

                4842     816      72
 
PfoI T'CCnGG_A

Cuts at:      0    3644    4841    5160    5730 
   Size:       3644    1197     319     570

  Fragments arranged by size: 

                3644    1197     570     319
 
PpuMI rG'GwC_Cy

Cuts at:      0     298    2071    5730 
   Size:        298    1773    3659

  Fragments arranged by size: 

                3659    1773     298
 
PvuII CAG'CTG

Cuts at:      0     246    2394    3309    4279    4458    5730 
   Size:        246    2148     915     970     179    1272

  Fragments arranged by size: 

                2148    1272     970     915     246     179
 
SacI G_AGCT'C

Cuts at:      0     367     598    3514    5730 
   Size:        367     231    2916    2216

  Fragments arranged by size: 

                2916    2216     367     231
 
SacII CC_GC'GG

Cuts at:      0     160    5730 
   Size:        160    5570
 
SanDI GG'GwC_CC

Cuts at:      0     298    5730 
   Size:        298    5432
 
SapI GCTCTTCn'nnn_

Cuts at:      0     219     586    1139    2738    5730 
   Size:        219     367     553    1599    2992

  Fragments arranged by size: 

                2992    1599     553     367     219
 
ScaI AGT'ACT

Cuts at:      0    3696    4111    5730 
   Size:       3696     415    1619

  Fragments arranged by size: 

                3696    1619     415
 
SfcI C'TryA_G

Cuts at:      0    1274    3227    3368    3465    3926    5730 
   Size:       1274    1953     141      97     461    1804

  Fragments arranged by size: 

                1953    1804    1274     461     141      97
 
SmaI CCC'GGG

Cuts at:      0     184    5730 
   Size:        184    5546
 
SmlI C'TyrA_G

Cuts at:      0    3172    4093    5730 
   Size:       3172     921    1637

  Fragments arranged by size: 

                3172    1637     921
 
SnaBI TAC'GTA

Cuts at:      0    4356    5730 
   Size:       4356    1374
 
SspI AAT'ATT

Cuts at:      0    4413    5157    5730 
   Size:       4413     744     573

  Fragments arranged by size: 

                4413     744     573
 
StuI AGG'CCT

Cuts at:      0     168     447    4798    5730 
   Size:        168     279    4351     932

  Fragments arranged by size: 

                4351     932     279     168
 
StyI C'CwwG_G

Cuts at:      0     419    2041    3491    4145    4247    4684    5576    5730 
   Size:        419    1622    1450     654     102     437     892     154

  Fragments arranged by size: 

                1622    1450     892     654     437     419     154     102
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     478    5730 
   Size:        478    5252
 
TatI w'GTAC_w

Cuts at:      0    1218    1240    1451    3694    4080    4109    5340    5730 
   Size:       1218      22     211    2243     386      29    1231     390

  Fragments arranged by size: 

                2243    1231    1218     390     386     211      29      22
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1747    1779    5730 
   Size:       1747      32    3951

  Fragments arranged by size: 

                3951    1747      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2075    5730 
   Size:       2075    3655
 
XmaI C'CCGG_G

Cuts at:      0     182    5730 
   Size:        182    5548
 
XmnI GAAnn'nnTTC

Cuts at:      0    2621    2639    2684    2868    3245    3500    5730 
   Size:       2621      18      45     184     377     255    2230

  Fragments arranged by size: 

                2621    2230     377     255     184      45      18
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AhdI    AlwNI    ApaLI
     ApoI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbsI
    BbvCI    BciVI    BfrBI    BglII     BlpI Bme1580I     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrGI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     HpaI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NsiI     NspI     PciI    PflMI     PfoI    PpuMI    PvuII     SacI
    SacII    SanDI     SapI     ScaI     SfcI     SmaI     SmlI    SnaBI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AleI     AlfI     AloI
     ApaI     AscI     AseI    AsiSI     BbeI     BcgI     BclI     BglI
    BmgBI     BmrI     BmtI     BplI    BsaBI    BsaHI    BsiWI    BsmBI
    BspEI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   DraIII     DrdI    EcoRV     FseI     FspI    FspAI    HaeII     KasI
     KpnI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SalI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SpeI     SphI     SrfI     SwaI     XbaI
     XcmI     XhoI     ZraI
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